##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551077_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	527244
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82918345206394	31.0	31.0	34.0	30.0	34.0
2	31.96770944761818	33.0	31.0	34.0	30.0	34.0
3	31.922123343271807	33.0	31.0	34.0	30.0	34.0
4	35.66672356631844	37.0	35.0	37.0	33.0	37.0
5	31.13295931295567	37.0	35.0	37.0	0.0	37.0
6	33.359264401301864	37.0	35.0	37.0	17.0	37.0
7	29.590872537193405	35.0	32.0	37.0	0.0	37.0
8	32.585719325397726	35.0	33.0	37.0	17.0	37.0
9	36.400300430161366	39.0	35.0	39.0	32.0	39.0
10	36.75155905045861	38.0	35.0	39.0	33.0	39.0
11	36.899710570437975	39.0	37.0	39.0	33.0	39.0
12	36.877906623878125	39.0	35.0	39.0	33.0	39.0
13	36.650742350790146	39.0	35.0	39.0	33.0	39.0
14	37.881809939989836	40.0	37.0	41.0	33.0	41.0
15	37.979466812329775	40.0	37.0	41.0	33.0	41.0
16	38.02645644141991	40.0	37.0	41.0	33.0	41.0
17	37.97760050375158	40.0	37.0	41.0	33.0	41.0
18	37.89196463117646	40.0	36.0	41.0	33.0	41.0
19	37.81959396408494	40.0	36.0	41.0	33.0	41.0
20	37.758874449021704	40.0	36.0	41.0	33.0	41.0
21	37.653187139161375	39.0	36.0	41.0	33.0	41.0
22	37.652637109194224	39.0	36.0	41.0	33.0	41.0
23	37.622173035634354	39.0	36.0	41.0	33.0	41.0
24	37.557172770102646	39.0	36.0	41.0	33.0	41.0
25	37.40762531199976	39.0	35.0	41.0	32.0	41.0
26	37.19090591832245	39.0	35.0	41.0	32.0	41.0
27	37.1676491339873	39.0	35.0	41.0	32.0	41.0
28	37.05712725038123	39.0	35.0	41.0	31.0	41.0
29	36.97090151808271	39.0	35.0	41.0	31.0	41.0
30	36.863983279089	39.0	35.0	41.0	31.0	41.0
31	36.74379983461168	39.0	35.0	40.0	31.0	41.0
32	36.562415124686105	39.0	35.0	40.0	30.0	41.0
33	36.4523636115347	39.0	35.0	40.0	30.0	41.0
34	36.267995463201096	39.0	35.0	40.0	30.0	41.0
35	36.076932122508744	39.0	35.0	40.0	29.0	41.0
36	35.97455637238167	38.0	35.0	40.0	29.0	41.0
37	35.90497568488214	38.0	35.0	40.0	29.0	41.0
38	35.72132257550584	38.0	35.0	40.0	27.0	41.0
39	35.68337619773767	38.0	35.0	40.0	27.0	41.0
40	35.40033077664231	38.0	34.0	40.0	26.0	41.0
41	35.17920355660757	38.0	34.0	40.0	25.0	41.0
42	35.100687727124445	38.0	34.0	40.0	24.0	41.0
43	34.912209527277696	38.0	34.0	40.0	24.0	41.0
44	34.64636866422377	38.0	34.0	40.0	23.0	41.0
45	34.50917981048622	38.0	33.0	40.0	23.0	41.0
46	34.29140208328592	38.0	33.0	40.0	22.0	41.0
47	34.1389223964616	37.0	33.0	40.0	22.0	41.0
48	33.98785382100128	37.0	33.0	40.0	21.0	41.0
49	33.81105901631882	37.0	33.0	40.0	20.0	41.0
50	33.60852849913892	37.0	33.0	40.0	20.0	41.0
51	31.42989583570415	35.0	28.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	26.0
10	28.0
11	11.0
12	14.0
13	9.0
14	14.0
15	32.0
16	60.0
17	136.0
18	258.0
19	470.0
20	797.0
21	1231.0
22	1993.0
23	2904.0
24	4137.0
25	5400.0
26	6763.0
27	8120.0
28	9663.0
29	11159.0
30	13464.0
31	16100.0
32	20599.0
33	28620.0
34	39341.0
35	46634.0
36	52655.0
37	78286.0
38	93550.0
39	84751.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.150988915947835	20.37519630379862	24.575149266753154	11.89866551350039
2	31.092245715456222	26.009779153484914	24.948979978909197	17.948995152149667
3	31.350001137993033	25.84173551524531	26.54861885578594	16.259644490975717
4	29.38848047583282	27.079302941332667	25.436230663601673	18.09598591923284
5	21.72087306825682	39.853654095636934	21.019679692893614	17.40579314321263
6	25.513045193496747	36.17641926698075	24.073673669117145	14.236861870405354
7	67.87255995326642	19.764283709250368	7.647692529455052	4.7154638080281615
8	80.85269818148713	6.4154357375332856	8.229586301598502	4.502279779381083
9	75.33684593850286	8.026644210270767	10.241747653837692	6.394762197388686
10	41.465810895903985	26.193754694221273	17.302994439007367	15.037439970867378
11	31.958637746470327	26.662228493828284	23.314632314450236	18.064501445251153
12	29.52105666446655	23.456881443885564	27.776323675565774	19.245738216082117
13	24.6377388837047	26.094180303616543	28.88909119876186	20.378989613916897
14	19.833701284414808	29.617975737988484	28.39937486249251	22.148948115104204
15	19.657312363915	27.274468746917936	33.944056262375675	19.124162626791392
16	22.70353005439607	25.071693561235403	31.426815667888114	20.797960716480414
17	21.869381159387306	25.161595011038536	28.030475453490222	24.93854837608394
18	22.950853874107622	26.901586362291464	28.3648557404162	21.782704023184714
19	24.69672485604388	28.057028624318153	26.145579655719175	21.100666863918793
20	26.434819552237677	27.58646850414609	26.53135929474778	19.447352648868453
21	24.48885146156239	27.675611291925563	27.634264211636356	20.201273034875694
22	23.277457875291134	25.692089431079346	26.303950353157173	24.726502340472344
23	22.97323440380545	27.260433499480317	27.03055890631283	22.73577319040141
24	23.73303442049601	25.05424433469134	28.116583593175076	23.096137651637573
25	21.542966823709705	26.943502439098406	27.56219131938912	23.951339417802764
26	20.754906646637988	29.149312272875555	27.274658412423847	22.821122668062603
27	22.154638080281615	28.447549900994606	26.538566583972507	22.859245434751273
28	19.87523803020992	27.803256177405526	28.38590861157263	23.93559718081192
29	21.669663381660104	27.861104156709228	27.593865458876728	22.875367002753944
30	21.676112008861175	26.461941719583344	27.504722671097255	24.357223600458234
31	23.66228918679018	27.47039321452686	24.87273444553186	23.9945831531511
32	23.61468314480582	27.63198822556539	26.788735386272773	21.964593243356017
33	22.377115718718468	27.303677234828655	26.30584700821631	24.013360038236566
34	22.228986958599815	26.64345160874282	27.726820978522277	23.40074045413509
35	21.59303851727094	26.138182700988537	27.868501111439866	24.400277670300657
36	21.459324335601732	28.01757819908809	27.151565499085812	23.371531966224364
37	22.423394102161428	27.1183740355509	28.335267921493656	22.122963940794016
38	21.257330571803568	26.743784661371205	27.04743913633915	24.951445630486074
39	23.1860391014407	25.139783477858447	28.00069796906176	23.67347945163909
40	23.542041256040847	25.01346625091988	28.84755445296675	22.59693804007253
41	20.285863850513238	26.024952393958017	29.342391757895776	24.346791997632973
42	21.551122440464	25.76700730591529	28.452670869654277	24.22919938396644
43	22.613818270098854	25.010621268331175	28.249728778326542	24.125831683243433
44	21.112615790791363	26.570051057954192	27.29817693515716	25.019156216097294
45	21.91452154979478	25.565582538634864	26.64098595716594	25.87890995440441
46	22.072133585209126	26.95867567957151	26.43121590762531	24.537974827594056
47	21.057043797558624	25.085159812155283	29.54628217675308	24.311514213533016
48	21.220535463656294	24.337688053349112	29.6126651038229	24.829111379171692
49	20.723611838162217	23.903733375818405	30.316324130762982	25.056330655256392
50	20.4671840741668	24.153522847106842	30.01323865231278	25.366054426413577
51	19.96627747304853	24.15390217811867	28.371683698629095	27.508136650203703
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	598.0
1	885.5
2	1173.0
3	1452.0
4	1731.0
5	1414.0
6	1097.0
7	1002.0
8	907.0
9	922.0
10	937.0
11	991.5
12	1046.0
13	1037.0
14	1028.0
15	1039.0
16	1050.0
17	1140.5
18	1231.0
19	1350.5
20	1470.0
21	1693.0
22	1916.0
23	2100.0
24	2284.0
25	2954.0
26	4327.0
27	5030.0
28	6444.5
29	7859.0
30	9225.5
31	10592.0
32	11646.0
33	12700.0
34	13984.5
35	15269.0
36	15762.5
37	16256.0
38	18691.5
39	21127.0
40	23139.0
41	25151.0
42	28057.0
43	30963.0
44	35061.5
45	39160.0
46	42499.5
47	45839.0
48	46761.5
49	47684.0
50	45491.5
51	43299.0
52	38811.5
53	34324.0
54	31050.5
55	27777.0
56	26085.5
57	24394.0
58	24115.0
59	23836.0
60	23432.5
61	23029.0
62	20030.0
63	17031.0
64	14515.0
65	11999.0
66	9985.5
67	7972.0
68	6670.5
69	5369.0
70	4525.5
71	3682.0
72	3114.5
73	2547.0
74	2159.0
75	1354.0
76	937.0
77	800.0
78	663.0
79	566.0
80	469.0
81	311.0
82	153.0
83	125.5
84	98.0
85	86.5
86	75.0
87	60.0
88	45.0
89	36.5
90	28.0
91	19.0
92	10.0
93	11.5
94	13.0
95	6.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	527244.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.70129810006129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.52895484384116	29.985975514414058
2	8.532912272974793	6.775353876220875
3	3.710529089531974	4.419384644773737
4	2.3166325952314595	3.6789328500641116
5	1.6337608717392351	3.2431213696567687
6	1.2228195009024414	2.9128512916737592
7	0.8614441394353091	2.394031540339038
8	0.6547686836019816	2.079613335541359
9	0.5211758480032689	1.8622221933717011
>10	4.923793251447626	38.85351371203805
>50	0.06623982975004238	1.650767565061098
>100	0.024603365335392716	1.6284142036130624
>500	0.002365708205326223	0.5158179032323762
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCG	635	0.12043759625524424	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	546	0.10355736622891867	No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	538	0.10204004218160852	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14907708764822358	0.0
2	0.0	0.0	0.0	0.7239532360728619	0.0
3	0.0	0.0	0.0	0.9815189931037622	0.0
4	0.0	0.0	0.0	1.2876391196485877	0.0
5	0.0	0.0	0.0	2.331178733186153	0.0
6	0.0	0.0	0.0	2.9223661151193756	0.0
7	0.0	0.0	0.0	3.5729188004036083	0.0
8	0.0	0.0	0.0	4.6058371456100025	0.0
9	0.0	0.0	0.0	4.963546289763373	0.0
10	0.0	0.0	0.0	6.01922449567942	0.0
11	0.0	0.0	0.0	7.128957370780891	0.0
12	0.0	0.0	0.0	8.16965200172975	0.0
13	0.0	0.0	0.0	8.574967187867477	0.0
14	0.0	0.0	0.0	8.739976178012457	0.0
15	0.0	0.0	0.0	9.079477433598106	0.0
16	0.0	0.0	0.0	9.752410648580163	0.0
17	0.0	0.0	0.0	10.51790063044814	0.0
18	0.0	0.0	0.0	11.382206340897193	0.0
19	0.0	0.0	0.0	11.875146990767083	0.0
20	0.0	0.0	0.0	12.340017145761735	0.0
21	0.0	0.0	0.0	12.892892095500375	0.0
22	0.0	0.0	0.0	13.558807686763624	0.0
23	0.0	0.0	0.0	14.201204755293563	0.0
24	0.0	0.0	0.0	14.728474861733845	0.0
25	0.0	0.0	0.0	15.173999135125293	0.0
26	0.0	0.0	0.0	15.592211575665157	0.0
27	0.0	0.0	0.0	15.97268058052818	0.0
28	0.0	0.0	0.0	16.409669906153507	0.0
29	0.0	0.0	0.0	16.826934019163804	0.0
30	0.0	0.0	0.0	17.289907519099316	0.0
31	0.0	0.0	0.0	17.770140580072983	0.0
32	0.0	0.0	0.0	18.226855118313342	0.0
33	0.0	0.0	0.0	18.666689426527377	0.0
34	0.0	0.0	0.0	19.060435016804362	0.0
35	0.0	0.0	0.0	19.52663283034041	0.0
36	0.0	0.0	0.0	19.98467502712217	0.0
37	0.0	0.0	0.0	20.441389565362527	0.0
38	0.0	0.0	0.0	20.885396514706663	0.0
39	0.0	0.0	0.0	21.313281896048128	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	20	7.0298096E-4	45.0	40
CGGAATC	20	7.0298096E-4	45.0	28
CGGGTAT	45	3.8380676E-10	45.0	6
CGAACGT	20	7.0298096E-4	45.0	38
CGACGGT	40	6.8030204E-9	45.0	28
GCGAATG	40	6.8030204E-9	45.0	1
CGTTGAA	20	7.0298096E-4	45.0	32
TACGGGT	35	1.2102464E-7	45.0	4
CACTCGC	20	7.0298096E-4	45.0	22
TGCGACG	20	7.0298096E-4	45.0	1
TCGACCT	20	7.0298096E-4	45.0	23
TATAACG	25	3.8879232E-5	45.0	1
TACGTAG	20	7.0298096E-4	45.0	1
TTTAACG	30	2.1632586E-6	44.999996	1
AAACGAG	30	2.1632586E-6	44.999996	37
TTAACGG	60	3.6379788E-12	41.249996	2
CGGGTTA	55	6.002665E-11	40.909092	6
AATGGGC	145	0.0	40.344826	4
ACCGGGT	45	1.9248546E-8	40.0	4
TACGAAT	40	3.4534787E-7	39.375	12
>>END_MODULE