FastQCFastQC Report
Sat 18 Jun 2016
SRR3551076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826895
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33390.40379975692197917No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.12190181341040882No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8410.1017057788473748No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGTC207.032136E-445.00000438
CGTTACG207.032136E-445.00000441
CCGTAAC207.032136E-445.00000439
GTCAACG207.032136E-445.0000041
CTCGATG207.032136E-445.0000041
TCGCGTA207.032136E-445.00000430
ATAACGC453.8380676E-1045.011
CTTACGT453.8380676E-1045.022
TATAGCG750.042.01
ACCGCTA650.041.53846422
TCGTTCA451.9272193E-840.016
TCGATGG850.039.7058832
TCGACGG403.4566438E-739.3750042
AATACGG1150.039.1304322
TCCGCAT356.246164E-638.57142624
CGCTAGA700.038.57142624
TTAACGG950.037.8947372
CGTTATT2500.037.81
TAACGGG2800.037.7678573
TACGGGA3650.037.6027374