##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551076_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	826895
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.952099117783998	33.0	31.0	34.0	30.0	34.0
2	32.084406121696226	33.0	31.0	34.0	30.0	34.0
3	31.945262699617242	33.0	31.0	34.0	30.0	34.0
4	35.71369520918617	37.0	35.0	37.0	35.0	37.0
5	31.20523041014881	37.0	35.0	37.0	0.0	37.0
6	33.434442099661986	37.0	35.0	37.0	17.0	37.0
7	29.543102812328048	35.0	32.0	37.0	0.0	37.0
8	32.5924041141862	35.0	33.0	37.0	17.0	37.0
9	36.46810538218274	39.0	35.0	39.0	32.0	39.0
10	36.86587414363371	38.0	37.0	39.0	33.0	39.0
11	37.01032537383828	39.0	37.0	39.0	34.0	39.0
12	37.00134478984635	39.0	37.0	39.0	33.0	39.0
13	36.83187587299476	39.0	37.0	39.0	33.0	39.0
14	38.108539778327355	40.0	37.0	41.0	33.0	41.0
15	38.18395322259779	40.0	37.0	41.0	33.0	41.0
16	38.21719444427648	40.0	37.0	41.0	33.0	41.0
17	38.1632226582577	40.0	37.0	41.0	33.0	41.0
18	38.06852986171158	40.0	37.0	41.0	33.0	41.0
19	37.968168872710564	40.0	37.0	41.0	33.0	41.0
20	37.8730987610277	40.0	36.0	41.0	33.0	41.0
21	37.75599441283355	40.0	36.0	41.0	33.0	41.0
22	37.72529281226758	40.0	36.0	41.0	33.0	41.0
23	37.65386899183088	40.0	36.0	41.0	33.0	41.0
24	37.57208714528447	40.0	35.0	41.0	33.0	41.0
25	37.4140561981872	39.0	35.0	41.0	32.0	41.0
26	37.232779252504855	39.0	35.0	41.0	32.0	41.0
27	37.19299669244584	39.0	35.0	41.0	32.0	41.0
28	37.066406254724	39.0	35.0	41.0	32.0	41.0
29	37.028100302940516	39.0	35.0	41.0	31.0	41.0
30	36.87707508208418	39.0	35.0	41.0	31.0	41.0
31	36.765230168280134	39.0	35.0	41.0	31.0	41.0
32	36.647938371860995	39.0	35.0	41.0	31.0	41.0
33	36.44858778925982	39.0	35.0	41.0	30.0	41.0
34	36.25044050332872	39.0	35.0	41.0	30.0	41.0
35	36.12054734881696	39.0	35.0	40.0	29.0	41.0
36	35.98647591290309	39.0	35.0	40.0	29.0	41.0
37	35.85044171267211	39.0	35.0	40.0	28.0	41.0
38	35.717780371147484	38.0	35.0	40.0	27.0	41.0
39	35.71286801830946	38.0	35.0	40.0	27.0	41.0
40	35.546168497814115	38.0	35.0	40.0	27.0	41.0
41	35.44812702942937	38.0	34.0	40.0	26.0	41.0
42	35.38055375833691	38.0	34.0	40.0	26.0	41.0
43	35.2173323094226	38.0	34.0	40.0	26.0	41.0
44	35.05873418027682	38.0	34.0	40.0	25.0	41.0
45	34.92859794774427	38.0	34.0	40.0	24.0	41.0
46	34.8301501399815	38.0	34.0	40.0	24.0	41.0
47	34.69691073231789	37.0	34.0	40.0	24.0	41.0
48	34.564886714758224	37.0	33.0	40.0	23.0	41.0
49	34.41729844780776	37.0	33.0	40.0	23.0	41.0
50	34.24297401725733	37.0	33.0	40.0	23.0	41.0
51	32.35408244093869	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	18.0
9	37.0
10	52.0
11	51.0
12	42.0
13	31.0
14	42.0
15	78.0
16	145.0
17	274.0
18	525.0
19	949.0
20	1525.0
21	2188.0
22	3195.0
23	4567.0
24	6233.0
25	7999.0
26	10026.0
27	11583.0
28	13171.0
29	14940.0
30	18164.0
31	22740.0
32	29687.0
33	41809.0
34	60559.0
35	72478.0
36	82780.0
37	125595.0
38	152401.0
39	142991.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.863725140435	19.960212602567438	24.38882808579082	12.787234171206743
2	32.89220517719904	24.376371848904636	25.025910182066646	17.705512791829676
3	32.35561951638358	24.58316956808301	26.632643806045508	16.428567109487904
4	29.032706691901634	26.025190622751378	26.0735643582317	18.868538327115292
5	22.252522992641147	39.62220112589869	21.30210002479154	16.82317585666862
6	26.28822280942562	35.254415615041815	24.476747350026304	13.98061422550626
7	69.02944146475672	20.039303660077763	7.079858990561075	3.8513958846044543
8	83.06350866796872	5.7981968690099706	7.7262530309168636	3.4120414321044388
9	77.04170420670097	8.207087961591254	9.500722582673738	5.250485249034037
10	42.31976248495879	26.997381771567124	16.500160237998777	14.182695505475301
11	32.97262651243507	26.29862316255389	22.85598534275815	17.872764982252885
12	29.97575266509049	22.795034436052944	27.382920443345288	19.84629245551128
13	25.36670314852551	24.342752102745816	28.668331529396113	21.622213219332565
14	22.19592572212917	26.674003349881183	28.86847785994594	22.261593068043705
15	22.175608753227436	25.376740698637672	31.348841146699403	21.09880940143549
16	25.11528065836654	23.702162910647665	30.337225403467187	20.84533102751861
17	24.151192110243745	24.250962939671904	28.276504272005514	23.321340678078837
18	24.852127537353592	25.158212348605325	28.10731713216309	21.88234298187799
19	27.074779748335644	26.020716052219445	25.800252752767882	21.10425144667703
20	29.06560083202825	25.34904673507519	26.06642923224835	19.51892320064821
21	27.009838008453315	25.955532443659713	26.168376879773124	20.866252668113848
22	25.371298653396142	24.88586821785112	25.833509695910607	23.90932343284214
23	25.34324188681755	25.64799642034357	26.458740226993754	22.55002146584512
24	24.956131068636285	24.50516691962099	28.442426184703017	22.096275827039708
25	25.18602724650651	25.342153477769248	26.4057709866428	23.066048289081444
26	22.938583496090796	26.96763192424673	26.82178511177356	23.27199946788891
27	23.325331511255964	26.37650487667721	27.301894436415747	22.99626917565108
28	22.158677945809323	27.39586041758627	28.41606249886624	22.029399137738164
29	23.98950289940077	25.868338785456434	27.348212288138157	22.793946027004637
30	23.99712176273892	25.207190755779152	28.09038632474498	22.705301156736947
31	25.054329751661335	26.280966749103573	25.973793528803533	22.690909970431555
32	26.095574407875244	26.155678774209544	26.2094945549314	21.539252262983812
33	25.44664074640674	26.49477865992659	26.262584729621054	21.79599586404562
34	23.1647307094613	26.975613590600982	27.888667847792043	21.970987852145676
35	23.93544525000151	25.677383464647868	27.678725835807448	22.70844544954317
36	23.97402330404707	28.981551466631196	25.910907672679116	21.13351755664262
37	24.440587982754764	28.304077301229295	26.80340309229104	20.4519316237249
38	24.379153338694756	28.127271298048722	26.018781102800236	21.474794260456285
39	24.817661250823868	26.66783569860744	26.308056040972556	22.20644700959614
40	24.802907261502366	26.054819535733074	27.63385919614945	21.50841400661511
41	22.366805942713402	26.41750161749678	27.9574794865128	23.25821295327702
42	23.588000894914106	26.639657997690154	26.565888051082666	23.206453056313077
43	23.780649296464485	25.65513154632692	27.364538423862765	23.19968073334583
44	23.349639313334826	26.385574952079764	27.007782124695396	23.25700360989001
45	23.5720375622056	26.06461521716784	26.748015165166073	23.615332055460488
46	22.637577927064502	27.58330864257251	26.537347547149277	23.24176588321371
47	23.988051687336363	26.59237267125814	27.60205346507114	21.817522176334357
48	23.379510094993925	25.917921864323766	27.559121774832356	23.143446265849956
49	22.965430919282376	26.028939587251106	27.74221636362537	23.263413129841155
50	21.556183070401925	26.11165867492245	28.343864698661864	23.988293556013762
51	22.631894013145562	25.558625944043683	27.368045519685086	24.441434523125668
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	673.0
1	1395.0
2	2117.0
3	5055.0
4	7993.0
5	6096.0
6	4199.0
7	3418.0
8	2637.0
9	2530.0
10	2423.0
11	2381.5
12	2340.0
13	2484.0
14	2628.0
15	2495.0
16	2362.0
17	2446.0
18	2530.0
19	2502.5
20	2475.0
21	2814.0
22	3153.0
23	3450.5
24	3748.0
25	4809.5
26	6693.5
27	7516.0
28	9091.0
29	10666.0
30	12636.0
31	14606.0
32	16566.0
33	18526.0
34	20796.5
35	23067.0
36	24737.5
37	26408.0
38	27825.5
39	29243.0
40	32403.5
41	35564.0
42	38025.0
43	40486.0
44	43930.0
45	47374.0
46	51424.0
47	55474.0
48	57210.5
49	58947.0
50	56022.0
51	53097.0
52	52544.5
53	51992.0
54	50527.5
55	49063.0
56	47543.0
57	46023.0
58	45354.5
59	44686.0
60	43364.0
61	42042.0
62	38516.0
63	34990.0
64	30378.5
65	25767.0
66	22568.5
67	19370.0
68	16974.5
69	14579.0
70	12598.5
71	10618.0
72	9162.5
73	7707.0
74	6559.0
75	4423.5
76	3436.0
77	2786.0
78	2136.0
79	1678.5
80	1221.0
81	975.0
82	729.0
83	597.0
84	465.0
85	356.0
86	247.0
87	199.5
88	152.0
89	117.0
90	82.0
91	56.5
92	31.0
93	23.5
94	16.0
95	10.5
96	5.0
97	3.0
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	826895.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.20196858162808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.1197295768391	24.277189641073534
2	8.008195585984739	5.317757164827959
3	3.6754069860600986	3.6609224182759115
4	2.502316749143824	3.3232736834541976
5	1.8070874805195365	2.9999430876231545
6	1.3447876368242244	2.6789758120079865
7	1.061287433094468	2.466578240677546
8	0.8666422858639252	2.3019383957411503
9	0.6999885417271009	2.091689781293057
>10	6.763164010385153	45.88116644984682
>50	0.12403792122186158	2.5600666015404774
>100	0.025482107929356713	1.6328883634478153
>500	0.0011242106439421963	0.2667565263054021
>1k	7.494737626281307E-4	0.5408538338850666
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3339	0.40379975692197917	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1008	0.12190181341040882	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	841	0.1017057788473748	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.043657296270989666	0.0
2	0.0	0.0	0.0	0.16471256931049286	0.0
3	0.0	0.0	0.0	0.24501297020782567	0.0
4	0.0	0.0	0.0	0.35433761239335104	0.0
5	0.0	0.0	0.0	0.6727577261925638	0.0
6	0.0	0.0	0.0	0.9659025632033088	0.0
7	0.0	0.0	0.0	1.220348411829797	0.0
8	0.0	0.0	0.0	1.7194444276480085	0.0
9	0.0	0.0	0.0	1.969536640081268	0.0
10	0.0	0.0	0.0	2.4715350800282985	0.0
11	0.0	0.0	0.0	2.998204125070293	0.0
12	0.0	0.0	0.0	3.4237720629584167	0.0
13	0.0	0.0	0.0	3.622588115782536	0.0
14	0.0	0.0	0.0	3.7096608396471136	0.0
15	0.0	0.0	0.0	3.853088965346265	0.0
16	0.0	0.0	0.0	4.120232919536337	0.0
17	0.0	0.0	0.0	4.42801081152988	0.0
18	0.0	0.0	0.0	4.767715368940434	0.0
19	0.0	0.0	0.0	4.965322078377545	0.0
20	0.0	0.0	0.0	5.186631918200013	0.0
21	0.0	0.0	0.0	5.45135718561607	0.0
22	0.0	0.0	0.0	5.745469497336421	0.0
23	0.0	0.0	0.0	6.031842011379921	0.0
24	0.0	0.0	0.0	6.268752380894793	0.0
25	0.0	0.0	0.0	6.477364115153677	0.0
26	0.0	0.0	0.0	6.6767848396713	0.0
27	0.0	0.0	0.0	6.866288948415458	0.0
28	1.209343387008024E-4	0.0	0.0	7.084817298447808	0.0
29	2.418686774016048E-4	0.0	0.0	7.3208811275917745	0.0
30	2.418686774016048E-4	0.0	0.0	7.574722304524759	0.0
31	2.418686774016048E-4	0.0	0.0	7.809818658959118	0.0
32	2.418686774016048E-4	0.0	0.0	8.04527781640958	0.0
33	2.418686774016048E-4	0.0	0.0	8.296095634875044	0.0
34	2.418686774016048E-4	0.0	0.0	8.542438882808579	0.0
35	2.418686774016048E-4	0.0	0.0	8.799182483870382	0.0
36	2.418686774016048E-4	0.0	0.0	9.054112069851675	0.0
37	2.418686774016048E-4	0.0	0.0	9.304688019639737	0.0
38	2.418686774016048E-4	0.0	0.0	9.57945083716796	0.0
39	2.418686774016048E-4	0.0	0.0	9.848408806438544	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGTC	20	7.032136E-4	45.000004	38
CGTTACG	20	7.032136E-4	45.000004	41
CCGTAAC	20	7.032136E-4	45.000004	39
GTCAACG	20	7.032136E-4	45.000004	1
CTCGATG	20	7.032136E-4	45.000004	1
TCGCGTA	20	7.032136E-4	45.000004	30
ATAACGC	45	3.8380676E-10	45.0	11
CTTACGT	45	3.8380676E-10	45.0	22
TATAGCG	75	0.0	42.0	1
ACCGCTA	65	0.0	41.538464	22
TCGTTCA	45	1.9272193E-8	40.0	16
TCGATGG	85	0.0	39.705883	2
TCGACGG	40	3.4566438E-7	39.375004	2
AATACGG	115	0.0	39.130432	2
TCCGCAT	35	6.246164E-6	38.571426	24
CGCTAGA	70	0.0	38.571426	24
TTAACGG	95	0.0	37.894737	2
CGTTATT	250	0.0	37.8	1
TAACGGG	280	0.0	37.767857	3
TACGGGA	365	0.0	37.602737	4
>>END_MODULE