##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551075_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	323096
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.814268824126575	31.0	31.0	34.0	30.0	34.0
2	31.943060266917573	33.0	31.0	34.0	30.0	34.0
3	31.797388392304455	33.0	31.0	34.0	30.0	34.0
4	35.58015883824003	37.0	35.0	37.0	33.0	37.0
5	31.219510609849703	37.0	35.0	37.0	0.0	37.0
6	33.38211243717038	37.0	35.0	37.0	17.0	37.0
7	29.41113167603437	35.0	32.0	37.0	0.0	37.0
8	32.42365736499369	35.0	33.0	37.0	17.0	37.0
9	36.28966622923218	39.0	35.0	39.0	32.0	39.0
10	36.70525478495556	38.0	35.0	39.0	33.0	39.0
11	36.86257335281155	39.0	37.0	39.0	33.0	39.0
12	36.92426709089558	39.0	37.0	39.0	33.0	39.0
13	36.75592084086463	39.0	35.0	39.0	33.0	39.0
14	38.02050164656944	40.0	37.0	41.0	33.0	41.0
15	38.081771362071954	40.0	37.0	41.0	33.0	41.0
16	38.13382090771783	40.0	37.0	41.0	33.0	41.0
17	38.06627441998663	40.0	37.0	41.0	33.0	41.0
18	37.97225901899126	40.0	37.0	41.0	33.0	41.0
19	37.86004159754376	40.0	37.0	41.0	33.0	41.0
20	37.81336506796742	40.0	36.0	41.0	33.0	41.0
21	37.729814049075195	40.0	36.0	41.0	33.0	41.0
22	37.69770904003764	39.0	36.0	41.0	33.0	41.0
23	37.64726892316835	39.0	36.0	41.0	33.0	41.0
24	37.596482160101026	39.0	36.0	41.0	33.0	41.0
25	37.474865055587195	39.0	36.0	41.0	33.0	41.0
26	37.31621870899052	39.0	35.0	41.0	32.0	41.0
27	37.24552145492361	39.0	35.0	41.0	32.0	41.0
28	37.075216034862706	39.0	35.0	41.0	31.0	41.0
29	37.026896030901035	39.0	35.0	41.0	31.0	41.0
30	36.90788805803848	39.0	35.0	41.0	31.0	41.0
31	36.64141926857652	39.0	35.0	41.0	30.0	41.0
32	36.53320684873846	39.0	35.0	40.0	30.0	41.0
33	36.39660967638101	39.0	35.0	40.0	30.0	41.0
34	36.239102310149306	39.0	35.0	40.0	30.0	41.0
35	36.08518830316686	39.0	35.0	40.0	29.0	41.0
36	35.83807908485404	38.0	35.0	40.0	27.0	41.0
37	35.734404016143806	38.0	35.0	40.0	27.0	41.0
38	35.530108698343525	38.0	35.0	40.0	26.0	41.0
39	35.614693465719164	38.0	35.0	40.0	27.0	41.0
40	35.51552170252804	38.0	35.0	40.0	26.0	41.0
41	35.43262374031248	38.0	34.0	40.0	26.0	41.0
42	35.32512008814717	38.0	34.0	40.0	26.0	41.0
43	35.15212506499616	38.0	34.0	40.0	25.0	41.0
44	34.96962203184193	38.0	34.0	40.0	24.0	41.0
45	34.85217087181519	38.0	34.0	40.0	24.0	41.0
46	34.71722336395375	38.0	34.0	40.0	23.0	41.0
47	34.50857020823533	38.0	33.0	40.0	23.0	41.0
48	34.35478928863248	37.0	33.0	40.0	23.0	41.0
49	34.174938717904276	37.0	33.0	40.0	22.0	41.0
50	33.98725456211157	37.0	33.0	40.0	21.0	41.0
51	31.829425310124545	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	13.0
10	13.0
11	12.0
12	6.0
13	9.0
14	16.0
15	28.0
16	36.0
17	76.0
18	153.0
19	322.0
20	542.0
21	842.0
22	1165.0
23	1733.0
24	2535.0
25	3345.0
26	4107.0
27	4869.0
28	5457.0
29	6476.0
30	7696.0
31	9679.0
32	12308.0
33	16781.0
34	22970.0
35	28489.0
36	32859.0
37	48977.0
38	58164.0
39	53405.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.36821254364028	20.300467972367343	23.93251541337559	13.398804070616782
2	31.814073835640183	25.90808923663555	25.23924777775027	17.038589149974
3	30.68654517542774	26.155384158268753	26.99012058335603	16.16795008294748
4	27.611298190011635	25.29960135687226	28.94186248050115	18.14723797261495
5	22.81303389704608	38.51208309604576	21.90370663827469	16.771176368633473
6	24.766632827394954	36.24216950999084	24.963787852526803	14.027409810087404
7	67.09120509074702	19.792878896674672	9.175910565280908	3.9400054472973975
8	79.88554485354197	6.676962884096367	9.217074801297446	4.220417461064204
9	73.80221358357888	8.059214598757025	10.661537128283854	7.477034689380247
10	39.32484462822195	27.935350484066657	18.346559536484513	14.39324535122688
11	29.016762819719215	25.670388986555082	26.885198207343947	18.427649986381756
12	26.83722484958031	23.32712258895189	30.040607126055413	19.795045435412383
13	23.430806942828138	24.391512120236712	32.40027731695842	19.777403619976724
14	20.195545596355263	27.763884418253397	30.25726099982668	21.783308985564663
15	19.233602396810856	26.125362121474733	35.0836902963825	19.557345185331915
16	21.582130388491347	26.012083096045757	31.07033203753683	21.335454477926067
17	21.262101666377795	25.307029489687277	29.79485973209201	23.63600911184292
18	21.84490058682249	26.382561220194617	29.9570406318865	21.815497561096393
19	22.79786812588209	27.661128580979028	27.64596280981504	21.89504048332384
20	24.161240002971255	27.037165424517788	29.23805927649986	19.563535296011093
21	23.629509495629783	27.95453982717211	28.443249065293287	19.97270161190482
22	22.72203927006215	24.852056354767623	27.74345705301211	24.682447322158122
23	21.176059127937208	27.840332285141255	28.73975536682596	22.243853220095573
24	22.480624953574168	25.11699309183648	29.451927600465496	22.95045435412385
25	21.246935895213806	28.601406393146313	27.369574367989703	22.782083343650182
26	21.615556986158914	27.23432044964964	27.994466041052814	23.155656523138635
27	23.0714710179018	27.54908757768589	27.227511327902544	22.151930076509768
28	20.141691633446406	27.079567682670163	30.72306682843489	22.055673855448536
29	23.238294500705674	25.918921930324114	28.41539356723698	22.42739000173323
30	23.557394706217348	26.258759006611037	29.451927600465496	20.73191868670612
31	23.632295045435413	26.340158962042242	28.234951840938916	21.79259415158343
32	24.30546958179612	28.33151756753411	26.447247876792034	20.91576497387773
33	23.216319607794585	26.97866887859955	27.63327308292272	22.171738430683142
34	22.69820734394731	25.609416396365166	30.00006190110679	21.69231435858073
35	22.96623913635576	25.62612969519895	27.75676579097234	23.65086537747295
36	23.31226632332186	28.50979275509446	28.166860623467947	20.01108029811573
37	21.780213930225074	27.884591576497385	30.27583133186421	20.059363161413327
38	21.200819570653923	27.594894396711812	28.91957808205611	22.284707950578156
39	21.660125783049	27.7570752965063	29.333077475425263	21.249721445019436
40	22.302968777081734	25.19994057493748	31.339292346547154	21.15779830143363
41	20.464505905365588	26.776252259390397	29.394669076683087	23.36457275856092
42	21.942704335553522	26.25783049000916	28.88181840691312	22.917646767524204
43	23.3360982494367	26.40144105776611	27.87406838834278	22.388392304454406
44	21.300789858122663	26.344492039517664	29.032857107485082	23.321860994874587
45	21.003974051056034	25.92975462401268	28.61100106469904	24.455270260232254
46	23.12811053061629	26.936576125981134	27.920494218436627	22.014819124965953
47	21.112300987941666	25.862591923143587	31.29998514373437	21.72512194518038
48	21.44316240374378	26.169621412830864	28.92855374254092	23.458662440884442
49	20.847673756406763	25.159395349988856	30.945601307351378	23.047329586253003
50	20.696944561368756	25.33457548220962	29.946826949265855	24.02165300715577
51	20.40817589818506	25.169299527075545	28.423440711119913	25.999083863619482
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	303.0
1	505.5
2	708.0
3	1888.5
4	3069.0
5	2286.5
6	1504.0
7	1250.0
8	996.0
9	1007.5
10	1019.0
11	1027.0
12	1035.0
13	963.0
14	891.0
15	939.0
16	987.0
17	974.0
18	961.0
19	1074.5
20	1188.0
21	1414.0
22	1640.0
23	1687.0
24	1734.0
25	2097.0
26	3052.5
27	3645.0
28	4507.0
29	5369.0
30	5994.0
31	6619.0
32	7269.5
33	7920.0
34	9066.5
35	10213.0
36	10732.0
37	11251.0
38	11994.0
39	12737.0
40	14431.0
41	16125.0
42	19105.5
43	22086.0
44	26941.5
45	31797.0
46	30144.0
47	28491.0
48	27072.5
49	25654.0
50	23006.5
51	20359.0
52	18104.0
53	15849.0
54	15037.5
55	14226.0
56	13784.5
57	13343.0
58	13173.5
59	13004.0
60	12587.0
61	12170.0
62	10982.5
63	9795.0
64	8471.0
65	7147.0
66	6036.5
67	4926.0
68	4191.0
69	3456.0
70	3069.0
71	2682.0
72	2313.5
73	1945.0
74	1698.5
75	1205.5
76	959.0
77	777.5
78	596.0
79	479.0
80	362.0
81	288.5
82	215.0
83	153.5
84	92.0
85	74.0
86	56.0
87	40.5
88	25.0
89	19.0
90	13.0
91	16.5
92	20.0
93	10.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	323096.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.18311651236081
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.19164304586553	33.070265354724654
2	9.719169054835012	8.782846956158842
3	4.585767725907715	6.215978323749364
4	2.8118291358080376	5.081888138242614
5	1.8396535953923943	4.156064137149902
6	1.2801064917628409	3.470352045932993
7	1.0310744287027929	3.261100923149591
8	0.8510286497063477	3.07617013080312
9	0.7691038799758572	3.1275459197152125
>10	3.8692276973736317	25.25634880749231
>50	0.029467208944004154	0.9276593776030271
>100	0.019187950010049217	1.9088852012425443
>500	0.0	0.0
>1k	0.0027411357157213164	1.6648946840358736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	1545	0.4781860499665734	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1377	0.4261891202614703	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG	1246	0.38564389531284815	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGC	1209	0.37419219055636715	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	449	0.1389679847475673	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	404	0.12504023571941467	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT	375	0.11606457523460519	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCT	330	0.10213682620645256	No Hit
GAATATGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	329	0.10182732067249363	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1259687523212915	0.0
2	0.0	0.0	0.0	0.5902270532597123	0.0
3	0.0	0.0	0.0	0.808737960234729	0.0
4	0.0	0.0	0.0	1.1708594349666972	0.0
5	0.0	0.0	0.0	2.4695446554584395	0.0
6	0.0	0.0	0.0	3.0956743506573896	0.0
7	0.0	0.0	0.0	3.7180899794488327	0.0
8	0.0	0.0	0.0	4.767004234035705	0.0
9	0.0	0.0	0.0	5.215787258276178	0.0
10	0.0	0.0	0.0	6.685319533513259	0.0
11	0.0	0.0	0.0	7.691212518879838	0.0
12	0.0	0.0	0.0	9.089249015772403	0.0
13	0.0	0.0	0.0	9.50708148661698	0.0
14	0.0	0.0	0.0	9.705165028350708	0.0
15	0.0	0.0	0.0	10.007551935028598	0.0
16	0.0	0.0	0.0	10.525664198875877	0.0
17	0.0	0.0	0.0	11.109391635922451	0.0
18	0.0	0.0	0.0	11.750996607819348	0.0
19	0.0	0.0	0.0	12.136021492064279	0.0
20	0.0	0.0	0.0	12.504642583009383	0.0
21	0.0	0.0	0.0	12.957449179191324	0.0
22	0.0	0.0	0.0	13.415826874984525	0.0
23	0.0	0.0	0.0	13.914130784658429	0.0
24	0.0	0.0	0.0	14.296370119097729	0.0
25	0.0	0.0	0.0	14.630017084705475	0.0
26	0.0	0.0	0.0	14.930237452645652	0.0
27	0.0	0.0	0.0	15.252432713496917	0.0
28	0.0	0.0	0.0	15.575865996484017	0.0
29	0.0	0.0	0.0	15.932106866070765	0.0
30	0.0	0.0	0.0	16.329511971674055	0.0
31	0.0	0.0	0.0	16.701537623492708	0.0
32	0.0	0.0	0.0	17.064897120360513	0.0
33	0.0	0.0	0.0	17.39730606383242	0.0
34	3.0950553395894717E-4	0.0	0.0	17.73838116225518	0.0
35	3.0950553395894717E-4	0.0	0.0	18.09493153737589	0.0
36	3.0950553395894717E-4	0.0	0.0	18.45055339589472	0.0
37	3.0950553395894717E-4	0.0	0.0	18.800294649268327	0.0
38	3.0950553395894717E-4	0.0	0.0	19.154368980117365	0.0
39	3.0950553395894717E-4	0.0	0.0	19.494206056404288	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACC	20	7.0257485E-4	45.000004	4
ATATGCG	20	7.0257485E-4	45.000004	1
CTTTCGT	20	7.0257485E-4	45.000004	15
TGTAACG	20	7.0257485E-4	45.000004	1
TTCGAAG	20	7.0257485E-4	45.000004	1
GTCATCG	25	3.8845596E-5	45.0	13
CGTTATT	105	0.0	45.0	1
CGTTTTA	45	3.8380676E-10	45.0	1
ATTACGG	30	2.1606393E-6	44.999996	2
TAGCCGT	30	2.1606393E-6	44.999996	44
TACGAAT	30	2.1606393E-6	44.999996	12
TACGGCT	165	0.0	42.272728	7
TGGGCGA	110	0.0	40.909092	6
GGCGATT	105	0.0	40.714287	8
GCGATTA	45	1.9208528E-8	40.0	9
GGGCGAT	455	0.0	39.56044	7
AGGCGAT	80	0.0	39.375004	7
CGTTTTT	550	0.0	39.272728	1
AACATAC	115	0.0	39.130432	16
CAACGGG	70	0.0	38.57143	3
>>END_MODULE