##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551073_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	407885
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.940257670666977	31.0	31.0	34.0	30.0	34.0
2	32.08440614388859	33.0	31.0	34.0	30.0	34.0
3	32.02663495838288	33.0	31.0	34.0	30.0	34.0
4	35.744202409993015	37.0	35.0	37.0	35.0	37.0
5	31.12760704610368	37.0	35.0	37.0	0.0	37.0
6	33.389646591563796	37.0	35.0	37.0	17.0	37.0
7	29.618397342388175	35.0	32.0	37.0	0.0	37.0
8	32.665810216114835	35.0	35.0	37.0	17.0	37.0
9	36.51620922563958	39.0	35.0	39.0	32.0	39.0
10	36.824043541684546	38.0	37.0	39.0	33.0	39.0
11	36.93146107358692	39.0	37.0	39.0	33.0	39.0
12	36.89059416257033	39.0	35.0	39.0	33.0	39.0
13	36.64741532539809	39.0	35.0	39.0	33.0	39.0
14	37.850864827095876	40.0	37.0	41.0	33.0	41.0
15	38.0056486509678	40.0	37.0	41.0	33.0	41.0
16	38.05816345293404	40.0	37.0	41.0	33.0	41.0
17	38.00280226044106	40.0	36.0	41.0	33.0	41.0
18	37.93811000649693	40.0	36.0	41.0	33.0	41.0
19	37.85965652083308	40.0	36.0	41.0	33.0	41.0
20	37.783311472596445	40.0	36.0	41.0	33.0	41.0
21	37.68216041286147	40.0	36.0	41.0	33.0	41.0
22	37.669637275212374	39.0	36.0	41.0	33.0	41.0
23	37.598291185015384	39.0	35.0	41.0	33.0	41.0
24	37.537313213283156	39.0	35.0	41.0	33.0	41.0
25	37.4074015960381	39.0	35.0	41.0	32.0	41.0
26	37.25236034666634	39.0	35.0	41.0	32.0	41.0
27	37.189592654792406	39.0	35.0	41.0	32.0	41.0
28	37.08836314157177	39.0	35.0	41.0	32.0	41.0
29	37.049131495396985	39.0	35.0	41.0	32.0	41.0
30	36.93874253772509	39.0	35.0	41.0	31.0	41.0
31	36.837025141890486	39.0	35.0	41.0	31.0	41.0
32	36.71159027667112	39.0	35.0	41.0	31.0	41.0
33	36.5588413400836	39.0	35.0	40.0	30.0	41.0
34	36.45021758583915	39.0	35.0	40.0	30.0	41.0
35	36.334814960099045	39.0	35.0	40.0	30.0	41.0
36	36.23666229451929	39.0	35.0	40.0	30.0	41.0
37	36.17061671794746	39.0	35.0	40.0	30.0	41.0
38	36.05382154283683	38.0	35.0	40.0	29.0	41.0
39	36.05112960760999	38.0	35.0	40.0	29.0	41.0
40	35.87896588499209	38.0	35.0	40.0	29.0	41.0
41	35.831893793593785	38.0	35.0	40.0	29.0	41.0
42	35.79559434644569	38.0	35.0	40.0	29.0	41.0
43	35.613113990463	38.0	35.0	40.0	28.0	41.0
44	35.38770241612219	38.0	34.0	40.0	27.0	41.0
45	35.26504774630104	38.0	34.0	40.0	26.0	41.0
46	35.17433590350221	38.0	34.0	40.0	26.0	41.0
47	35.05976194270444	38.0	34.0	40.0	26.0	41.0
48	35.00368241048335	38.0	34.0	40.0	26.0	41.0
49	34.88783848388639	37.0	34.0	40.0	26.0	41.0
50	34.698326734251076	37.0	34.0	40.0	24.0	41.0
51	32.717994042438434	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	16.0
9	21.0
10	34.0
11	21.0
12	15.0
13	13.0
14	15.0
15	36.0
16	52.0
17	102.0
18	238.0
19	400.0
20	588.0
21	896.0
22	1298.0
23	1910.0
24	2528.0
25	3333.0
26	4208.0
27	4911.0
28	5828.0
29	7188.0
30	9001.0
31	11223.0
32	14871.0
33	21871.0
34	31767.0
35	37417.0
36	40244.0
37	61823.0
38	75201.0
39	70808.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1707834316045	20.347401841205244	24.864361278301484	10.61745344888878
2	32.81218971033502	24.25389509297964	25.477279135050317	17.45663606163502
3	34.11991124949434	24.33872292435368	25.373083099403015	16.168282726748963
4	29.316596589725048	26.138985253196367	24.951885948245216	19.592532208833372
5	21.308211873444723	40.98704291650833	20.864214178015864	16.840531032031087
6	25.36450224940853	36.835627689177095	23.814555573262073	13.985314488152298
7	70.27078710911164	19.773465560145628	5.887934099072043	4.067813231670692
8	84.56574769849344	4.785662625494931	7.043161675472254	3.605428000539368
9	78.8923348492835	7.395957193816885	8.57962415877024	5.132083798129375
10	42.68678671684421	26.813930397048185	16.18667026245143	14.312612623656177
11	33.984824153866896	26.194883361731865	22.288144942814764	17.532147541586475
12	31.16025350282555	25.163710359537617	25.491008495041495	18.18502764259534
13	25.105115412432426	26.925971781261875	27.382718168111108	20.586194638194588
14	20.842884636601003	29.71450286232639	28.19569241330277	21.246920087769837
15	20.674945143851822	27.027961312624882	32.567758069063586	19.729335474459713
16	24.117336994495997	24.703531632690588	31.6093997082511	19.569731664562315
17	22.715716439682755	24.980080169655665	27.651421356509804	24.652782034151784
18	25.186020569523272	25.716807433467764	28.335437684641505	20.761734312367455
19	25.97864594187087	28.489157483114113	25.125954619561885	20.40624195545313
20	28.104490236218542	26.582492614339827	26.08014513894848	19.232872010493153
21	25.884746926216952	27.040955171187957	26.662171935717176	20.41212596687792
22	24.175686774458487	25.068095173884796	27.289064319599888	23.46715373205683
23	23.474263579195117	27.50383073660468	26.947791656962135	22.07411402723807
24	23.628964046238522	25.55499711928607	29.374210868259436	21.441827966215968
25	22.70125157826348	27.073317234024298	27.750223715017714	22.475207472694507
26	21.416820917660615	29.447515843926595	27.125783002562	22.00988023585079
27	22.325655515647792	29.06088725988943	26.9656888583792	21.64776836608358
28	19.681037547347906	29.49507826961031	28.52102921166505	22.302854971376735
29	22.219743310001594	27.596013582259705	28.086102700516076	22.098140407222626
30	23.400468269242555	27.08704659401547	28.18539539330939	21.327089743432584
31	25.830074653394952	27.311374529585546	25.854836534807607	21.003714282211895
32	25.157336013827425	27.84804540495483	25.690084214913515	21.304534366304228
33	23.6939333390539	28.789487232921047	25.189943243806468	22.32663618421859
34	22.145702832906334	27.824019024970276	28.030204591980585	22.000073550142808
35	21.72107334175074	28.59506968876031	26.956127339813918	22.727729629675032
36	23.5880211334077	29.419321622516147	25.89577944763843	21.09687779643772
37	23.543155546293686	28.593598685904116	26.53419468722802	21.32905108057418
38	22.583816516910403	29.089571815585273	25.370631427976022	22.955980239528298
39	23.508341812030352	27.05443936403643	26.155901786042634	23.28131703789058
40	23.407578116380844	26.318202434509725	28.222415631857018	22.05180381725241
41	20.478811429692193	27.762727239295387	27.271412285325518	24.4870490456869
42	22.92288267526386	26.561163072924966	25.842333010529927	24.673621241281243
43	23.56129791485345	26.227245424568203	26.190960687448666	24.020495973129684
44	22.509530872672446	27.304755016732656	26.32237027593562	23.86334383465928
45	22.283486767103472	27.56880002942006	25.961484241881905	24.186228961594566
46	22.5847971854812	28.408007158880565	26.702379347119898	22.304816308518333
47	21.64261985608689	27.938757247753653	27.596503916545107	22.822118979614352
48	22.346739889919952	26.65824926143398	28.123122939063705	22.87188790958236
49	22.670850852568737	25.601333709256284	28.107677409073634	23.62013802910134
50	20.320923789793692	27.18830062395038	27.855400419235814	24.635375167020115
51	21.026269659340254	26.826433921325865	26.622700025742553	25.52459639359133
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	251.0
1	605.0
2	959.0
3	1274.0
4	1589.0
5	1268.5
6	948.0
7	798.0
8	648.0
9	720.5
10	793.0
11	827.5
12	862.0
13	939.0
14	1016.0
15	1106.0
16	1196.0
17	1107.5
18	1019.0
19	1183.5
20	1348.0
21	1393.5
22	1439.0
23	1540.0
24	1641.0
25	2072.5
26	2923.0
27	3342.0
28	4183.0
29	5024.0
30	6335.0
31	7646.0
32	8781.0
33	9916.0
34	11012.5
35	12109.0
36	12881.0
37	13653.0
38	15013.0
39	16373.0
40	17977.5
41	19582.0
42	22305.0
43	25028.0
44	27227.5
45	29427.0
46	31141.5
47	32856.0
48	33447.5
49	34039.0
50	33523.5
51	33008.0
52	30425.5
53	27843.0
54	25271.0
55	22699.0
56	21439.0
57	20179.0
58	19366.0
59	18553.0
60	18032.5
61	17512.0
62	15813.0
63	14114.0
64	11864.0
65	9614.0
66	7812.5
67	6011.0
68	5135.0
69	4259.0
70	3623.5
71	2988.0
72	2610.5
73	2233.0
74	1911.5
75	1238.5
76	887.0
77	704.0
78	521.0
79	428.5
80	336.0
81	227.5
82	119.0
83	102.0
84	85.0
85	74.5
86	64.0
87	49.0
88	34.0
89	24.5
90	15.0
91	12.5
92	10.0
93	6.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	407885.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.894176620808295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.11718167770711	24.443524931791245
2	8.630210317713836	5.850277455666296
3	4.074682340410815	4.14324008758718
4	2.5850794987370698	3.5047656443609925
5	1.9181445823415546	3.2506965629065605
6	1.478087151137412	3.0059128176959287
7	1.147844337846086	2.7233667104104997
8	0.8786967284553474	2.382616168831358
9	0.7222141106424319	2.203096736173911
>10	6.3111025325596595	44.942975509264386
>50	0.1186672088973054	2.5297709442644676
>100	0.018089513551418506	1.0197564310471796
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	443	0.1086090442158942	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	431	0.10566703850349976	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06889196709856946	0.0
2	0.0	0.0	0.0	0.3226399597925886	0.0
3	0.0	0.0	0.0	0.4334555082927786	0.0
4	0.0	0.0	0.0	0.584723635338392	0.0
5	0.0	0.0	0.0	1.042695857901124	0.0
6	0.0	0.0	0.0	1.3528322934160364	0.0
7	0.0	0.0	0.0	1.650710371795972	0.0
8	0.0	0.0	0.0	2.2628927271167116	0.0
9	0.0	0.0	0.0	2.522769898378219	0.0
10	0.0	0.0	0.0	3.0927835051546393	0.0
11	0.0	0.0	0.0	3.8273042646824473	0.0
12	0.0	0.0	0.0	4.359316964340439	0.0
13	0.0	0.0	0.0	4.5949225884746925	0.0
14	0.0	0.0	0.0	4.690782941270211	0.0
15	0.0	0.0	0.0	4.903588021133408	0.0
16	0.0	0.0	0.0	5.330424016573299	0.0
17	0.0	0.0	0.0	5.8161001262610785	0.0
18	0.0	0.0	0.0	6.392978413033085	0.0
19	0.0	0.0	0.0	6.705321352832294	0.0
20	0.0	0.0	0.0	7.012025448349412	0.0
21	0.0	0.0	0.0	7.370704978118832	0.0
22	0.0	0.0	0.0	7.7921472964193335	0.0
23	0.0	0.0	0.0	8.183188889025093	0.0
24	0.0	0.0	0.0	8.500435171678292	0.0
25	0.0	0.0	0.0	8.792429238633439	0.0
26	0.0	0.0	0.0	9.078048959878396	0.0
27	0.0	0.0	0.0	9.369062358262745	0.0
28	0.0	0.0	0.0	9.669146940926977	0.0
29	0.0	0.0	0.0	9.964573347879917	0.0
30	0.0	0.0	0.0	10.306581511945769	0.0
31	0.0	0.0	0.0	10.635350650305845	0.0
32	0.0	0.0	0.0	10.969268298662612	0.0
33	0.0	0.0	0.0	11.284308077031517	0.0
34	0.0	0.0	0.0	11.592483175404832	0.0
35	0.0	0.0	0.0	11.9266459909043	0.0
36	0.0	0.0	0.0	12.260073304975666	0.0
37	0.0	0.0	0.0	12.58565527048065	0.0
38	0.0	0.0	0.0	12.904617723132747	0.0
39	4.903342853990708E-4	0.0	0.0	13.270652267183152	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTT	25	3.8863654E-5	45.0	23
CTATGCG	20	7.027929E-4	45.0	1
TGGACGC	20	7.027929E-4	45.0	37
TAGCGAC	20	7.027929E-4	45.0	21
ATAGCGA	20	7.027929E-4	45.0	20
ACCGACA	25	3.8863654E-5	45.0	19
TTCGTTA	20	7.027929E-4	45.0	38
ACGATAT	25	3.8863654E-5	45.0	32
GGTATAC	25	3.8863654E-5	45.0	8
GCGATCA	20	7.027929E-4	45.0	9
TTAACGG	25	3.8863654E-5	45.0	2
CGGCTTA	20	7.027929E-4	45.0	23
ACACTCG	20	7.027929E-4	45.0	34
CCACGTT	25	3.8863654E-5	45.0	39
CGATCAT	20	7.027929E-4	45.0	10
ATACCCG	20	7.027929E-4	45.0	30
CGCAGTA	75	0.0	42.000004	37
TTACGGG	125	0.0	41.399998	3
CGTATGG	55	6.002665E-11	40.909092	2
CGATTTG	55	6.002665E-11	40.909092	10
>>END_MODULE