##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551070_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	602943
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.922398966403126	31.0	31.0	34.0	30.0	34.0
2	32.055461295678036	33.0	31.0	34.0	30.0	34.0
3	31.989556226707997	33.0	31.0	34.0	30.0	34.0
4	35.72239166886422	37.0	35.0	37.0	35.0	37.0
5	31.2026941186812	37.0	35.0	37.0	0.0	37.0
6	33.41561142595569	37.0	35.0	37.0	17.0	37.0
7	29.53328921639359	35.0	32.0	37.0	0.0	37.0
8	32.550710431997715	35.0	33.0	37.0	17.0	37.0
9	36.41558820651372	39.0	35.0	39.0	32.0	39.0
10	36.75225021270668	38.0	35.0	39.0	33.0	39.0
11	36.917587566320535	39.0	37.0	39.0	33.0	39.0
12	36.95585984081414	39.0	37.0	39.0	33.0	39.0
13	36.78593167181641	39.0	37.0	39.0	33.0	39.0
14	38.061226351412984	40.0	37.0	41.0	33.0	41.0
15	38.14272161713462	40.0	37.0	41.0	33.0	41.0
16	38.20187314555439	40.0	37.0	41.0	33.0	41.0
17	38.15422850916256	40.0	37.0	41.0	33.0	41.0
18	38.05071457832664	40.0	37.0	41.0	33.0	41.0
19	37.965316787822395	40.0	37.0	41.0	33.0	41.0
20	37.92329954904527	40.0	37.0	41.0	33.0	41.0
21	37.83162089948801	40.0	36.0	41.0	33.0	41.0
22	37.81469392629154	40.0	36.0	41.0	33.0	41.0
23	37.75664034577066	40.0	36.0	41.0	33.0	41.0
24	37.70181095062054	40.0	36.0	41.0	33.0	41.0
25	37.56243459166124	39.0	36.0	41.0	33.0	41.0
26	37.386769561965224	39.0	36.0	41.0	32.0	41.0
27	37.35504517010729	39.0	36.0	41.0	32.0	41.0
28	37.22170752459188	39.0	36.0	41.0	32.0	41.0
29	37.19123864113192	39.0	36.0	41.0	32.0	41.0
30	37.077808350043036	39.0	35.0	41.0	31.0	41.0
31	36.95238355864485	39.0	35.0	41.0	31.0	41.0
32	36.845885929515724	39.0	35.0	41.0	31.0	41.0
33	36.685472756131176	39.0	35.0	41.0	30.0	41.0
34	36.57091632210673	39.0	35.0	41.0	30.0	41.0
35	36.45422701648415	39.0	35.0	41.0	30.0	41.0
36	36.314321585954225	39.0	35.0	40.0	30.0	41.0
37	36.23284953967456	39.0	35.0	40.0	30.0	41.0
38	36.0656148259454	39.0	35.0	40.0	29.0	41.0
39	36.03244585309059	39.0	35.0	40.0	29.0	41.0
40	35.87908309740722	39.0	35.0	40.0	28.0	41.0
41	35.79075136455685	38.0	35.0	40.0	28.0	41.0
42	35.75861233980658	38.0	35.0	40.0	28.0	41.0
43	35.56303166302619	38.0	35.0	40.0	27.0	41.0
44	35.37845202614509	38.0	34.0	40.0	26.0	41.0
45	35.23544182451741	38.0	34.0	40.0	26.0	41.0
46	35.1038771492496	38.0	34.0	40.0	25.0	41.0
47	34.9860965298544	38.0	34.0	40.0	24.0	41.0
48	34.88873243407752	38.0	34.0	40.0	24.0	41.0
49	34.746181645694534	38.0	34.0	40.0	24.0	41.0
50	34.5569332424458	37.0	33.0	40.0	24.0	41.0
51	32.526723089910654	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	23.0
10	38.0
11	22.0
12	22.0
13	20.0
14	26.0
15	61.0
16	96.0
17	187.0
18	303.0
19	533.0
20	866.0
21	1405.0
22	2043.0
23	2905.0
24	4038.0
25	5223.0
26	6381.0
27	7619.0
28	8899.0
29	10779.0
30	13114.0
31	16719.0
32	21091.0
33	29927.0
34	42707.0
35	51264.0
36	60367.0
37	93744.0
38	114475.0
39	108024.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.49060690645716	20.492152657879767	24.149214768228504	10.868025667434566
2	33.30862121295048	25.415835327717545	24.614930432893324	16.66061302643865
3	33.06912925434079	24.80997374544526	25.443366951768244	16.677530048445707
4	28.42208965026545	26.035960281485977	26.035960281485977	19.505989786762594
5	22.175064641267912	39.88702082949798	20.962843917252545	16.975070611981565
6	25.74356116581501	36.826532524633336	24.09862955536427	13.331276754187376
7	69.95885183176519	19.74780368956933	6.500282779632569	3.7930616990329105
8	83.49445967529269	6.012176938781941	6.777091698551936	3.716271687373433
9	77.42572680999696	7.964102742713656	8.649905546627128	5.960264900662252
10	39.6272947857426	27.92701797682368	17.259177069805936	15.186510167627787
11	31.127983905609653	26.74149961107435	23.4866314062855	18.643885077030497
12	28.71266438121016	24.21522432468741	27.63213106379873	19.439980230303693
13	24.14523429246214	26.26135472175645	29.940143595663272	19.65326739011814
14	21.151087250370267	28.606352507616805	28.653123097871607	21.58943714414132
15	20.83596625219963	26.812153055927347	32.94672962452504	19.405151067347994
16	23.262729644427417	24.987270770205473	31.245407940717445	20.50459164464966
17	23.871410730367547	24.792393310810475	27.879418120784223	23.45677783803776
18	24.02217125001866	26.060672401868835	28.350275233313926	21.56688111479858
19	25.144831269290798	27.47059008894705	25.998477468019367	21.38610117374279
20	26.195511018454482	26.811323790142684	27.23607372504532	19.757091466357515
21	25.58666407935742	26.87186019242283	26.791753117624715	20.749722610595033
22	24.52520387499316	25.26822601804814	26.67698273302783	23.52958737393087
23	23.503216721978696	27.83181826474476	27.007859781107	21.657105232169542
24	23.246973594518884	25.64239074008654	29.005561056351926	22.105074609042646
25	22.886242978191966	28.02470548625658	27.05861084712817	22.030440688423283
26	22.53048795657301	27.948081327754032	27.226620095100202	22.294810620572758
27	23.066193653463095	28.043612746146817	27.140708159809467	21.749485440580617
28	21.547476295437544	27.622511580696685	29.086994956405498	21.743017167460273
29	23.789810977157046	26.520749059197968	27.471585207888637	22.217854755756345
30	22.48288810053355	26.542641675912982	28.311797300905724	22.662672922647747
31	24.15037574032703	26.926591734210366	26.287061961080898	22.635970564381708
32	25.089933874346332	27.437419457560665	26.45158829275736	21.021058375335645
33	23.286612499025612	26.994923234866313	27.699467445513093	22.01899682059498
34	23.321607515138247	26.426212759746775	27.83248167737249	22.41969804774249
35	22.775784775675312	25.780380566653893	28.28111446687332	23.162720190797472
36	23.358924475447928	27.498619272468545	27.656179771553862	21.486276480529668
37	22.56962930160894	28.406997012984643	27.74773071418028	21.275642971226137
38	22.067923501889897	27.226951801414064	28.44132861646955	22.263796080226488
39	22.80746272864931	26.000965265373345	28.234675582932383	22.956896423044963
40	23.340348921871552	25.588156757769802	29.487530330396073	21.58396398996257
41	21.19636516221268	26.217735341483355	28.990634272228057	23.595265224075906
42	22.428488265059883	25.85534619358712	28.505016228731407	23.21114931262159
43	22.94677938047212	26.354398342795253	27.961515433465518	22.737306843267106
44	22.461658896446266	26.57299280363152	27.921710675801858	23.043637624120354
45	22.21702548997169	26.12120880414898	27.451516975899874	24.21024872997945
46	22.147367164060284	27.402590294604963	27.424648764476906	23.025393776857843
47	22.178713410720416	25.80409756809516	29.290165073647096	22.72702394753733
48	21.843358327404083	25.7253173185525	29.490183980906988	22.94114037313643
49	21.684305149906375	25.818195086434372	29.513071716563587	22.984428047095662
50	20.87941977931579	26.264340078581228	29.317862550854723	23.53837759124826
51	21.272160054930566	25.635259054338473	27.936969166239596	25.155611724491372
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	266.0
1	650.5
2	1035.0
3	1645.0
4	2255.0
5	1781.0
6	1307.0
7	1150.5
8	994.0
9	1016.5
10	1039.0
11	1087.0
12	1135.0
13	1262.0
14	1389.0
15	1433.0
16	1477.0
17	1490.5
18	1504.0
19	1582.5
20	1661.0
21	1771.0
22	1881.0
23	2253.0
24	2625.0
25	3482.5
26	5347.5
27	6355.0
28	7591.5
29	8828.0
30	10277.0
31	11726.0
32	13062.0
33	14398.0
34	16992.5
35	19587.0
36	21166.0
37	22745.0
38	24262.0
39	25779.0
40	28746.5
41	31714.0
42	34405.0
43	37096.0
44	40114.0
45	43132.0
46	46421.5
47	49711.0
48	48085.0
49	46459.0
50	46491.0
51	46523.0
52	42720.0
53	38917.0
54	36735.0
55	34553.0
56	31958.0
57	29363.0
58	27693.5
59	26024.0
60	25106.0
61	24188.0
62	22034.0
63	19880.0
64	17217.5
65	14555.0
66	12390.0
67	10225.0
68	8308.0
69	6391.0
70	5243.0
71	4095.0
72	3549.0
73	3003.0
74	2462.0
75	1630.5
76	1340.0
77	1025.5
78	711.0
79	522.0
80	333.0
81	279.5
82	226.0
83	168.0
84	110.0
85	94.5
86	79.0
87	54.0
88	29.0
89	24.0
90	19.0
91	15.0
92	11.0
93	9.0
94	7.0
95	4.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	602943.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.30898672656833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.70494312314885	25.671378716911686
2	8.345614867478496	5.89350409161699
3	4.020155313397546	4.258428317984913
4	2.6208828713008265	3.7016287405860466
5	1.9003102604346944	3.3549014881025108
6	1.3906476273843655	2.946141516998898
7	1.1480850190796514	2.837640308975875
8	0.88616933300672	2.5031792973300946
9	0.7056772457546633	2.2425073653233434
>10	6.183803041001935	42.992855108286534
>50	0.07016574574875023	1.5942343303397686
>100	0.0211909970371502	1.2578621932129168
>500	0.0014127331358100132	0.39473428244381553
>1k	9.418220905400089E-4	0.3510042418866447
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	1100	0.18243847262510718	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC	1011	0.16767754165816667	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	946	0.15689708645759218	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	843	0.13981421129360488	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.317063138638312E-4	0.0	0.0	0.058546164396966215	0.0
2	3.317063138638312E-4	0.0	0.0	0.31296490713052477	0.0
3	3.317063138638312E-4	0.0	0.0	0.4176182491545635	0.0
4	3.317063138638312E-4	0.0	0.0	0.5945835676009175	0.0
5	3.317063138638312E-4	0.0	0.0	1.224659710785265	0.0
6	3.317063138638312E-4	0.0	0.0	1.5439270378792025	0.0
7	3.317063138638312E-4	0.0	0.0	1.887077219571336	0.0
8	3.317063138638312E-4	0.0	0.0	2.4516413657675766	0.0
9	3.317063138638312E-4	0.0	0.0	2.6700699734469096	0.0
10	3.317063138638312E-4	0.0	0.0	3.478935819803862	0.0
11	3.317063138638312E-4	0.0	0.0	4.0969046825321795	0.0
12	3.317063138638312E-4	0.0	0.0	4.82251224410931	0.0
13	3.317063138638312E-4	0.0	0.0	5.04757497806592	0.0
14	3.317063138638312E-4	0.0	0.0	5.15322343903155	0.0
15	4.975594707957469E-4	0.0	0.0	5.337983855853704	0.0
16	4.975594707957469E-4	0.0	0.0	5.709992486851991	0.0
17	4.975594707957469E-4	0.0	0.0	6.1355716875392865	0.0
18	4.975594707957469E-4	0.0	0.0	6.615384870543318	0.0
19	4.975594707957469E-4	0.0	0.0	6.881579187419043	0.0
20	4.975594707957469E-4	0.0	0.0	7.155900308984432	0.0
21	4.975594707957469E-4	0.0	0.0	7.493743189654744	0.0
22	4.975594707957469E-4	0.0	0.0	7.878522513736788	0.0
23	4.975594707957469E-4	0.0	0.0	8.25102870420587	0.0
24	4.975594707957469E-4	0.0	0.0	8.557857044529914	0.0
25	4.975594707957469E-4	0.0	0.0	8.834831816606213	0.0
26	4.975594707957469E-4	0.0	0.0	9.090079825124432	0.0
27	4.975594707957469E-4	0.0	0.0	9.352459519390722	0.0
28	4.975594707957469E-4	0.0	0.0	9.622966018346677	0.0
29	4.975594707957469E-4	0.0	0.0	9.895462755185813	0.0
30	4.975594707957469E-4	0.0	0.0	10.225344684323394	0.0
31	4.975594707957469E-4	0.0	0.0	10.546933955614378	0.0
32	4.975594707957469E-4	0.0	0.0	10.849781820172057	0.0
33	4.975594707957469E-4	0.0	0.0	11.13836631323359	0.0
34	4.975594707957469E-4	0.0	0.0	11.410199637444999	0.0
35	4.975594707957469E-4	0.0	0.0	11.711388970433358	0.0
36	4.975594707957469E-4	0.0	0.0	12.01539780708956	0.0
37	4.975594707957469E-4	0.0	0.0	12.317084699548714	0.0
38	4.975594707957469E-4	0.0	0.0	12.626566690383669	0.0
39	4.975594707957469E-4	0.0	0.0	12.952136437441018	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAC	20	7.030614E-4	45.000004	32
TCTACGG	25	3.8885904E-5	45.000004	2
TTAACGG	20	7.030614E-4	45.000004	2
TCGATCA	30	2.1637788E-6	44.999996	17
GTATACG	30	2.1637788E-6	44.999996	1
GCTACGA	110	0.0	42.954544	10
CGTTTTA	75	0.0	42.0	1
TACGGGA	210	0.0	41.785713	4
ATAGGCG	50	1.0804797E-9	40.500004	5
ATCACGG	45	1.925764E-8	40.0	2
TTTGACG	45	1.925764E-8	40.0	1
ATTTGCG	45	1.925764E-8	40.0	1
TATCACG	40	3.45457E-7	39.375004	1
AGGCGAT	170	0.0	38.382355	7
ACAACGA	135	0.0	38.333332	13
CGAATAT	125	0.0	37.8	14
TTACGGG	125	0.0	37.8	3
ACACGAC	90	0.0	37.5	26
TCACGGT	30	1.1392782E-4	37.499996	14
CCGGATA	30	1.1392782E-4	37.499996	21
>>END_MODULE