FastQCFastQC Report
Sat 18 Jun 2016
SRR3551069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498749
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18110.36310849746064655No Hit
GTGAATGATACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGC10370.20792021638138622No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8370.1678198853531536No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.1176944715678628No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG253.887618E-545.0000042
CCTCGTG207.029442E-445.015
CGTTCCT207.029442E-445.034
TCATCCG207.029442E-445.041
TTACGTT207.029442E-445.034
GTATACG207.029442E-445.01
ACCGTTC207.029442E-445.032
ACGTACG351.2100827E-745.01
TAGCACG302.1630203E-644.9999961
GCTACGA1800.043.7510
AGGCGAT1250.043.1999977
CGGTTTT2100.042.8571471
CGAATAT1950.041.5384614
TACGAAT1900.041.4473712
AGCTACG1900.041.447379
GGCGATA1550.040.645168
GCATATC1450.040.34482628
ATGGGAC2200.039.886365
TATACGG403.4529694E-739.3752
CGTTTTT9900.038.8636361
Produced by FastQC (version 0.11.5)