##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551069_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	498749
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.836115962137267	31.0	31.0	34.0	30.0	34.0
2	31.947019442645498	33.0	31.0	34.0	30.0	34.0
3	31.782146931622922	33.0	31.0	34.0	30.0	34.0
4	35.58795907360215	37.0	35.0	37.0	33.0	37.0
5	31.117433819416178	37.0	35.0	37.0	0.0	37.0
6	33.35210697164305	37.0	35.0	37.0	17.0	37.0
7	29.616127551132934	35.0	32.0	37.0	0.0	37.0
8	32.618076427220906	35.0	33.0	37.0	17.0	37.0
9	36.40051007621068	39.0	35.0	39.0	32.0	39.0
10	36.73947817439233	38.0	35.0	39.0	33.0	39.0
11	36.90735420020892	39.0	37.0	39.0	33.0	39.0
12	36.9541913868499	39.0	37.0	39.0	33.0	39.0
13	36.76870730567881	39.0	35.0	39.0	33.0	39.0
14	38.00434086083381	40.0	37.0	41.0	33.0	41.0
15	38.10699169321643	40.0	37.0	41.0	33.0	41.0
16	38.147994281692796	40.0	37.0	41.0	33.0	41.0
17	38.12468796929919	40.0	37.0	41.0	33.0	41.0
18	38.03029379507528	40.0	37.0	41.0	33.0	41.0
19	37.923450473083655	40.0	37.0	41.0	33.0	41.0
20	37.82209488139325	40.0	36.0	41.0	33.0	41.0
21	37.72619493973923	40.0	36.0	41.0	33.0	41.0
22	37.69921343200688	39.0	36.0	41.0	33.0	41.0
23	37.67307002119303	39.0	36.0	41.0	33.0	41.0
24	37.62798321400143	39.0	36.0	41.0	33.0	41.0
25	37.51145165203339	39.0	36.0	41.0	33.0	41.0
26	37.35923881551642	39.0	35.0	41.0	32.0	41.0
27	37.30985926788826	39.0	35.0	41.0	32.0	41.0
28	37.19322545007609	39.0	35.0	41.0	32.0	41.0
29	37.15440632462421	39.0	35.0	41.0	32.0	41.0
30	37.06113896970219	39.0	35.0	41.0	31.0	41.0
31	36.95880492993469	39.0	35.0	41.0	31.0	41.0
32	36.855253845120494	39.0	35.0	41.0	31.0	41.0
33	36.689406896053924	39.0	35.0	41.0	30.0	41.0
34	36.52093137028846	39.0	35.0	41.0	30.0	41.0
35	36.396588263836115	39.0	35.0	41.0	30.0	41.0
36	36.27469729262615	39.0	35.0	40.0	30.0	41.0
37	36.165864994215525	39.0	35.0	40.0	29.0	41.0
38	36.060731951342255	39.0	35.0	40.0	29.0	41.0
39	36.043386553156	39.0	35.0	40.0	29.0	41.0
40	35.90926899101552	39.0	35.0	40.0	28.0	41.0
41	35.7929058504378	39.0	35.0	40.0	28.0	41.0
42	35.717543293319885	38.0	35.0	40.0	27.0	41.0
43	35.53567024695789	38.0	35.0	40.0	27.0	41.0
44	35.36597166109606	38.0	34.0	40.0	26.0	41.0
45	35.26464213462082	38.0	34.0	40.0	25.0	41.0
46	35.10407439413412	38.0	34.0	40.0	24.0	41.0
47	34.95019939889604	38.0	34.0	40.0	24.0	41.0
48	34.77406069987108	38.0	34.0	40.0	23.0	41.0
49	34.58832398661451	38.0	34.0	40.0	23.0	41.0
50	34.40801485316261	38.0	33.0	40.0	23.0	41.0
51	32.357993700238	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	13.0
9	21.0
10	28.0
11	26.0
12	13.0
13	13.0
14	29.0
15	33.0
16	70.0
17	134.0
18	239.0
19	439.0
20	733.0
21	1046.0
22	1736.0
23	2378.0
24	3473.0
25	4726.0
26	6006.0
27	6879.0
28	7655.0
29	9064.0
30	10876.0
31	13660.0
32	18124.0
33	24555.0
34	35822.0
35	42499.0
36	48469.0
37	75584.0
38	94710.0
39	89679.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.47888216317225	20.70199639498024	25.061102879404267	11.758018562443233
2	33.5054305873295	24.45318186101626	25.14731859111497	16.89406896053927
3	32.13760829595648	23.418192317177578	27.355042315874318	17.08915707099162
4	28.149429873543607	26.785617615273416	26.68195825956543	18.382994251617546
5	21.533677260505783	39.09581773597541	21.719542294821643	17.65096270869716
6	24.74932280565976	35.036862229297704	26.408273500297746	13.805541464744792
7	68.47472375884462	19.404750686216914	7.808937962782883	4.311587592155573
8	81.34091496925308	5.966929257001016	8.788990053112888	3.9031657206330235
9	75.37198069570064	8.052948477089679	10.94558585581124	5.629484971398439
10	39.305742968908206	27.846271370970165	18.767155422868015	14.080830237253608
11	29.78191434970296	27.068525450677594	25.43965000431079	17.709910195308662
12	29.686876565166042	23.00114887448396	28.980910237414008	18.331064322935987
13	25.20406055951992	25.430627429829432	29.699508169439937	19.66580384121071
14	19.750014536369996	29.32777810080822	29.2896827863314	21.63252457649038
15	19.16715622487464	27.390731610489443	34.35335208692148	19.088760077714443
16	21.492574421201844	25.938097118991717	32.41550358998214	20.153824869824298
17	21.64295066255772	25.1523311324935	29.901814339477372	23.302903865471407
18	22.009668189810906	27.4484760871701	29.14492059131948	21.396935131699514
19	23.991226047571026	28.690583840769605	27.28145820843751	20.03673190322186
20	25.101002708777358	27.020004050133434	28.86522078239756	19.013772458691648
21	24.19232920767761	27.821409165732664	28.210181875051376	19.77607975153835
22	21.830018706804424	26.882660416361738	28.14802636195762	23.139294514876223
23	21.568364046845208	28.106522519343397	28.98111073906915	21.344002694742244
24	21.941497627062912	25.934688590854314	30.371188714162834	21.752625067919936
25	21.670218887656915	28.252487724286162	27.611082929489584	22.466210458567335
26	21.014578475345314	28.71564654766225	28.66331561567041	21.606459361322027
27	22.074630726076645	29.40156270990017	28.258703275595543	20.265103288427646
28	20.32745930317655	28.022913329149535	29.999659147186257	21.64996822048766
29	21.137285488291706	26.589125993235076	29.666224894686504	22.607363623786714
30	22.15082135503029	27.215092160585787	28.854393693019937	21.779692791363992
31	22.59633603275395	27.77549428670534	27.82421618890464	21.803953491636076
32	23.10440722688166	28.178703115194214	27.90582036254709	20.81106929537703
33	23.18220186907643	27.983615004741864	27.155142165698575	21.67904096048313
34	20.73126963663085	27.576596644805303	29.474745814026694	22.21738790453715
35	21.981597958091147	25.519048659746684	28.913140677976294	23.586212704185876
36	22.341698930724675	28.52296445707159	27.79193542242691	21.343401189776824
37	21.819592620737087	28.506122318039733	28.993942844998184	20.680342216224997
38	21.16555622166661	28.346322498892228	28.01188573811677	22.476235541324392
39	22.038740929806373	26.461205937255013	27.96697336736515	23.533079765573465
40	21.919442444997383	26.941206899662955	29.766475722257084	21.37287493308257
41	20.263699776841655	26.38461430499109	30.538006091240284	22.813679826926972
42	21.898991276172985	27.001357396205307	28.99634886485988	22.10330246276183
43	22.443553771536383	27.06391391260935	28.101309476309726	22.391222839544543
44	21.44305051238198	27.42662140675971	28.183916158227884	22.94641192263042
45	21.808565029704322	26.642860436812903	26.520153423866518	25.02842110961626
46	20.8118713019976	28.597751574439243	27.353839305943474	23.236537817619684
47	21.60325133483977	26.142207803925423	29.98722804456751	22.2673128166673
48	21.157536155460964	25.74701904164219	29.819408159214355	23.27603664368249
49	20.62500375940603	25.963159825884365	29.641763692759284	23.77007272195032
50	19.292068756027582	26.184914656470486	30.283168487555866	24.239848099946066
51	19.76064112409248	25.203459054554493	28.704217953319205	26.33168186803382
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	226.0
1	555.5
2	885.0
3	2607.5
4	4330.0
5	3423.5
6	2517.0
7	2013.0
8	1509.0
9	1474.5
10	1440.0
11	1403.5
12	1367.0
13	1388.0
14	1409.0
15	1396.0
16	1383.0
17	1406.5
18	1430.0
19	1524.5
20	1619.0
21	1883.0
22	2147.0
23	2338.0
24	2529.0
25	3163.5
26	4602.0
27	5406.0
28	6455.0
29	7504.0
30	8951.0
31	10398.0
32	12057.0
33	13716.0
34	15263.5
35	16811.0
36	17651.5
37	18492.0
38	20558.5
39	22625.0
40	24504.0
41	26383.0
42	28680.5
43	30978.0
44	34334.0
45	37690.0
46	40415.5
47	43141.0
48	43382.5
49	43624.0
50	41389.0
51	39154.0
52	34564.5
53	29975.0
54	26899.5
55	23824.0
56	22276.5
57	20729.0
58	20845.0
59	20961.0
60	20371.5
61	19782.0
62	17383.0
63	14984.0
64	12035.5
65	9087.0
66	7445.5
67	5804.0
68	4788.5
69	3773.0
70	3379.0
71	2985.0
72	2361.5
73	1738.0
74	1415.0
75	850.0
76	608.0
77	492.0
78	376.0
79	297.0
80	218.0
81	183.0
82	148.0
83	111.0
84	74.0
85	54.0
86	34.0
87	27.5
88	21.0
89	16.0
90	11.0
91	7.0
92	3.0
93	5.5
94	8.0
95	5.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	498749.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.253908016468976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.84927757668615	30.53229842687547
2	8.33820609521021	6.712907623579055
3	3.7304154590466085	4.504914022550282
4	2.342976058435305	3.7725577096417524
5	1.6544860230248233	3.329976409268741
6	1.1861681929593633	2.8648743198868463
7	0.8805541912266506	2.4812023192007673
8	0.6849519550483091	2.2057594395372178
9	0.5339222433001561	1.934321118277613
>10	4.709577013876763	37.310948905098286
>50	0.06277391867727865	1.6201976731923293
>100	0.024714141211478916	1.8800500815387693
>500	9.885656484591567E-4	0.28333065045096917
>1k	9.885656484591567E-4	0.5666613009019383
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1811	0.36310849746064655	No Hit
GTGAATGATACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGC	1037	0.20792021638138622	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	837	0.1678198853531536	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	587	0.1176944715678628	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.005016551411632E-4	0.0	0.0	0.07919815378075946	0.0
2	2.005016551411632E-4	0.0	0.0	0.401805316902891	0.0
3	2.005016551411632E-4	0.0	0.0	0.5614046343952569	0.0
4	2.005016551411632E-4	0.0	0.0	0.7773449169822897	0.0
5	2.005016551411632E-4	0.0	0.0	1.4161431902620356	0.0
6	2.005016551411632E-4	0.0	0.0	1.9687257518310814	0.0
7	2.005016551411632E-4	0.0	0.0	2.561809647738642	0.0
8	2.005016551411632E-4	0.0	0.0	3.335746036583532	0.0
9	2.005016551411632E-4	0.0	0.0	3.6856214248048618	0.0
10	2.005016551411632E-4	0.0	0.0	4.5521895783249695	0.0
11	2.005016551411632E-4	0.0	0.0	5.529835648793281	0.0
12	2.005016551411632E-4	0.0	0.0	6.553797601599201	0.0
13	2.005016551411632E-4	0.0	0.0	6.899662956717708	0.0
14	2.005016551411632E-4	0.0	0.0	7.1065806648233885	0.0
15	2.005016551411632E-4	0.0	0.0	7.349388169199337	0.0
16	2.005016551411632E-4	0.0	0.0	7.968336778620108	0.0
17	2.005016551411632E-4	0.0	0.0	8.619766656173747	0.0
18	2.005016551411632E-4	0.0	0.0	9.418966253566424	0.0
19	2.005016551411632E-4	0.0	0.0	9.813754012539373	0.0
20	2.005016551411632E-4	0.0	0.0	10.240221033024628	0.0
21	2.005016551411632E-4	0.0	0.0	10.753104266875724	0.0
22	2.005016551411632E-4	0.0	0.0	11.295260742377428	0.0
23	2.005016551411632E-4	0.0	0.0	11.866690459529744	0.0
24	2.005016551411632E-4	0.0	0.0	12.273909321121446	0.0
25	2.005016551411632E-4	0.0	0.0	12.694962796917888	0.0
26	2.005016551411632E-4	0.0	0.0	13.047444706656053	0.0
27	2.005016551411632E-4	0.0	0.0	13.427395343148557	0.0
28	2.005016551411632E-4	0.0	0.0	13.827596646810319	0.0
29	2.005016551411632E-4	0.0	0.0	14.240028551435692	0.0
30	2.005016551411632E-4	0.0	0.0	14.707798912880026	0.0
31	2.005016551411632E-4	0.0	0.0	15.145093022742902	0.0
32	2.005016551411632E-4	0.0	0.0	15.579379607778662	0.0
33	4.010033102823264E-4	0.0	0.0	15.962939274063707	0.0
34	4.010033102823264E-4	0.0	0.0	16.3579275346918	0.0
35	4.010033102823264E-4	0.0	0.0	16.776173987316266	0.0
36	6.015049654234895E-4	0.0	0.0	17.191613416768757	0.0
37	8.020066205646528E-4	0.0	0.0	17.61647642401288	0.0
38	8.020066205646528E-4	0.0	0.0	18.046752975945818	0.0
39	8.020066205646528E-4	0.0	0.0	18.47843303946474	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGG	25	3.887618E-5	45.000004	2
CCTCGTG	20	7.029442E-4	45.0	15
CGTTCCT	20	7.029442E-4	45.0	34
TCATCCG	20	7.029442E-4	45.0	41
TTACGTT	20	7.029442E-4	45.0	34
GTATACG	20	7.029442E-4	45.0	1
ACCGTTC	20	7.029442E-4	45.0	32
ACGTACG	35	1.2100827E-7	45.0	1
TAGCACG	30	2.1630203E-6	44.999996	1
GCTACGA	180	0.0	43.75	10
AGGCGAT	125	0.0	43.199997	7
CGGTTTT	210	0.0	42.857147	1
CGAATAT	195	0.0	41.53846	14
TACGAAT	190	0.0	41.44737	12
AGCTACG	190	0.0	41.44737	9
GGCGATA	155	0.0	40.64516	8
GCATATC	145	0.0	40.344826	28
ATGGGAC	220	0.0	39.88636	5
TATACGG	40	3.4529694E-7	39.375	2
CGTTTTT	990	0.0	38.863636	1
>>END_MODULE