FastQCFastQC Report
Sat 18 Jun 2016
SRR3551068_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551068_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809957
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39950.49323606067976444No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCG16030.19791174099365766No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13290.1640827846416538No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGC12980.16025542096679204No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTC12380.15284762030576932No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.10222764912211389No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTC253.8897815E-545.018
CGTTGCT207.032051E-445.016
CGAGCAT351.2113378E-745.022
CGGTCTA453.8380676E-1045.031
TATCGCG207.032051E-445.01
TAATGCG253.8897815E-545.01
CGTTATT3500.042.428571
CGTGCGG556.002665E-1140.9090922
CGTTTTT16750.040.7014921
GTTAGCG451.9270374E-840.01
TATTACG451.9270374E-840.01
GCGAGAC2200.038.86363621
CCCGATC356.246006E-638.57142641
CACAACG3000.038.25000412
CGGTTTT4700.037.819151
CGTTTTA2150.037.674421
TCGCTAT301.1396242E-437.50000423
TCGAACG301.1396242E-437.5000041
ACGGGTC900.037.55
CGCACGG850.037.0588232