##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551068_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	809957
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.953146895452473	33.0	31.0	34.0	30.0	34.0
2	32.08061539069358	33.0	31.0	34.0	30.0	34.0
3	31.863169279356807	33.0	31.0	34.0	30.0	34.0
4	35.66130918061082	37.0	35.0	37.0	33.0	37.0
5	31.15782689698342	37.0	35.0	37.0	0.0	37.0
6	33.41907409899538	37.0	35.0	37.0	17.0	37.0
7	29.578466511185162	35.0	32.0	37.0	0.0	37.0
8	32.64759487232038	35.0	35.0	37.0	17.0	37.0
9	36.55744564217606	39.0	35.0	39.0	32.0	39.0
10	36.92262305282873	38.0	37.0	39.0	34.0	39.0
11	37.05546343818252	39.0	37.0	39.0	34.0	39.0
12	37.09476675922302	39.0	37.0	39.0	34.0	39.0
13	36.966220428985736	39.0	37.0	39.0	33.0	39.0
14	38.29594163640786	40.0	38.0	41.0	34.0	41.0
15	38.330401490449496	40.0	38.0	41.0	34.0	41.0
16	38.348302440746856	40.0	38.0	41.0	34.0	41.0
17	38.26698701289081	40.0	37.0	41.0	34.0	41.0
18	38.17030163329658	40.0	37.0	41.0	33.0	41.0
19	38.06367375058182	40.0	37.0	41.0	34.0	41.0
20	38.01072032218995	40.0	37.0	41.0	34.0	41.0
21	37.91653384068537	40.0	36.0	41.0	33.0	41.0
22	37.89071518611482	40.0	36.0	41.0	33.0	41.0
23	37.834531956634734	40.0	36.0	41.0	33.0	41.0
24	37.7829193401625	40.0	36.0	41.0	33.0	41.0
25	37.671139332087996	40.0	36.0	41.0	33.0	41.0
26	37.512607459408336	40.0	36.0	41.0	33.0	41.0
27	37.494622554036816	40.0	36.0	41.0	33.0	41.0
28	37.385296997248005	39.0	35.0	41.0	33.0	41.0
29	37.36293531631926	39.0	35.0	41.0	33.0	41.0
30	37.24714274955337	39.0	35.0	41.0	32.0	41.0
31	37.1453249987345	39.0	35.0	41.0	32.0	41.0
32	37.00927827032793	39.0	35.0	41.0	31.0	41.0
33	36.83460973854167	39.0	35.0	41.0	31.0	41.0
34	36.62679129879734	39.0	35.0	41.0	30.0	41.0
35	36.500247544005425	39.0	35.0	41.0	30.0	41.0
36	36.381157271311935	39.0	35.0	41.0	30.0	41.0
37	36.29668612037429	39.0	35.0	41.0	30.0	41.0
38	36.19706354781797	39.0	35.0	41.0	29.0	41.0
39	36.1722943316744	39.0	35.0	41.0	29.0	41.0
40	36.03207701149567	39.0	35.0	40.0	29.0	41.0
41	35.9443044507301	39.0	35.0	40.0	28.0	41.0
42	35.91201878618248	39.0	35.0	40.0	28.0	41.0
43	35.780721692633065	39.0	35.0	40.0	28.0	41.0
44	35.63290643824302	38.0	35.0	40.0	27.0	41.0
45	35.48427138724649	38.0	35.0	40.0	27.0	41.0
46	35.3687343896034	38.0	34.0	40.0	26.0	41.0
47	35.240851057525276	38.0	34.0	40.0	26.0	41.0
48	35.15188584085328	38.0	34.0	40.0	25.0	41.0
49	34.99814434593441	38.0	34.0	40.0	24.0	41.0
50	34.85855668881187	38.0	34.0	40.0	24.0	41.0
51	32.995998553009606	36.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	19.0
9	38.0
10	46.0
11	29.0
12	27.0
13	25.0
14	42.0
15	64.0
16	80.0
17	158.0
18	299.0
19	610.0
20	979.0
21	1550.0
22	2421.0
23	3603.0
24	5425.0
25	7519.0
26	9626.0
27	10829.0
28	11766.0
29	13517.0
30	15919.0
31	20210.0
32	26171.0
33	37433.0
34	54292.0
35	65823.0
36	77568.0
37	125212.0
38	160577.0
39	158065.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.0832920759991	20.734434050202665	25.22961095465562	12.952662919142622
2	33.02237032336284	25.088615815407483	24.965646324434505	16.923367536795165
3	31.9981184186321	24.547722903808474	27.509114681396667	15.945043996162758
4	27.616157400948442	25.7616145054614	28.418916065914612	18.203312027675544
5	22.046602473958494	39.18417891320157	22.612928834493683	16.15628977834626
6	25.38258203830574	35.13544546191959	25.215906523432725	14.266065976341954
7	69.51023325929648	19.08175372272849	7.897085894683298	3.5109271232917303
8	84.55004401468226	4.8364295882374	7.87152898240277	2.741997414677569
9	78.24970955248241	7.419282752047331	9.381856073840957	4.949151621629296
10	38.27635294219323	33.14472249761407	16.22863929813558	12.350285262057122
11	28.482993541632457	26.350658121357064	28.104454927854196	17.06189340915629
12	27.876171204150346	23.497914086797202	30.02035910548338	18.60555560356908
13	24.052881819652157	24.325365420633442	31.641803206836904	19.9799495528775
14	20.750607748312564	27.410467469260713	30.30704099106496	21.531883791361764
15	20.272804605676598	27.274904717164	33.29473046099978	19.157560216159624
16	24.80440319671291	25.801369702342225	31.21573120548375	18.178495895461115
17	23.60841377999079	25.714204581230852	30.28790417269065	20.389477466087705
18	24.44895222832817	26.1189174240114	29.635647324487596	19.796483023172833
19	23.7576809633104	28.466572916833854	27.628750662072182	20.14699545778356
20	26.673638230177648	26.681046030838672	28.313354906495036	18.33196083248864
21	25.5337011717906	27.795302713600844	28.413607142107544	18.25738897250101
22	24.365244080858613	24.551920657516387	29.554284980560695	21.528550281064305
23	23.606438366481182	27.165763120758264	29.68145222524159	19.54634628751897
24	23.28247055090579	25.505057675901316	30.635330023692614	20.57714174950028
25	22.638609210118563	27.63961543637502	28.32372582742047	21.398049526085952
26	21.893137536930972	27.767153071088956	28.890299114644357	21.44941027733571
27	21.947337945100788	27.1290945074862	29.60157144144689	21.321996105966118
28	20.64405888213819	27.967781005658328	30.37877319413253	21.00938691807096
29	22.155373680331177	27.293053828783503	29.70515718735686	20.84641530352846
30	23.321731894409208	26.05113604796304	30.130241481955217	20.496890575672534
31	24.277832033058544	25.855693573856392	28.206065260254558	21.66040913283051
32	24.411666298334357	27.325771615036352	28.44509029491689	19.8174717917124
33	24.029794174258633	26.92945427967164	27.9927206012171	21.048030944852627
34	21.77078536267975	26.668823159747983	30.582981565688055	20.977409911884212
35	22.414646703466975	26.92723193947333	29.597991004460734	21.060130352598964
36	21.85906165389027	29.199574792242057	28.584480410688467	20.356883143179203
37	22.651325934586648	29.20142674240731	27.708014129145127	20.439233193860908
38	22.375879213340955	29.053641119219908	27.366144128638926	21.20433553880021
39	23.327905061626726	27.201567490619873	27.52430067275176	21.946226775001637
40	23.528656459540446	26.045209807434222	29.495516428649914	20.930617304375417
41	20.52417597477397	27.33848833950444	29.693922023020974	22.44341366270061
42	21.493239764580093	27.24194000422245	28.77387318092195	22.49094705027551
43	22.444524832799768	26.245837742003587	28.370888824962314	22.938748600234334
44	22.3904478879743	26.928713499605532	27.730360994472548	22.95047761794762
45	22.730095548282193	26.315717994905903	27.86493603981446	23.089250416997444
46	22.406374659395496	27.53627661715375	27.323302348149348	22.734046375301407
47	21.525834087488597	26.38448707770906	29.863066804780996	22.226612030021347
48	21.90906430835217	26.015825531478832	29.56502629151918	22.510083868649815
49	22.08771576762717	26.105212992788505	29.579224576119472	22.227846663464852
50	20.479605707463485	26.663390772596568	29.61107811896187	23.245925400978077
51	20.664800723989053	25.287391799811594	29.493417551795957	24.554389924403395
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	443.0
1	882.5
2	1322.0
3	5367.0
4	9412.0
5	7264.0
6	5116.0
7	4008.5
8	2901.0
9	2805.5
10	2710.0
11	2647.0
12	2584.0
13	2544.5
14	2505.0
15	2519.5
16	2534.0
17	2553.0
18	2572.0
19	2485.0
20	2398.0
21	2827.0
22	3256.0
23	3568.0
24	3880.0
25	5325.0
26	8341.0
27	9912.0
28	11329.5
29	12747.0
30	15215.5
31	17684.0
32	20676.5
33	23669.0
34	25334.0
35	26999.0
36	29339.0
37	31679.0
38	32953.5
39	34228.0
40	38050.5
41	41873.0
42	44936.5
43	48000.0
44	51114.5
45	54229.0
46	58396.0
47	62563.0
48	61202.0
49	59841.0
50	59548.0
51	59255.0
52	55780.0
53	52305.0
54	48091.5
55	43878.0
56	42055.5
57	40233.0
58	38403.5
59	36574.0
60	34952.0
61	33330.0
62	29368.5
63	25407.0
64	21509.5
65	17612.0
66	14249.0
67	10886.0
68	8702.0
69	6518.0
70	5547.0
71	4576.0
72	3782.0
73	2988.0
74	2449.0
75	1547.5
76	1185.0
77	936.5
78	688.0
79	503.5
80	319.0
81	260.5
82	202.0
83	165.0
84	128.0
85	92.5
86	57.0
87	48.5
88	40.0
89	27.0
90	14.0
91	14.0
92	14.0
93	10.0
94	6.0
95	4.5
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	809957.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.20981855070171
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.96790931960261	24.6200911929696
2	8.463867652224527	5.790947532395096
3	3.997936520716943	4.10306048852851
4	2.6588592560777484	3.6383637080909406
5	1.9488067351163354	3.3334162399357625
6	1.4686918758294802	3.014620954740975
7	1.1991828870126342	2.8716680281666194
8	0.9430991968871526	2.5810601920657597
9	0.7412660488751476	2.2822719326832948
>10	6.487703505876445	42.86395849642537
>50	0.0966546106831677	2.102194234743048
>100	0.023420151988055606	1.425117091149436
>500	7.434968885096878E-4	0.16977791510659254
>1k	0.0018587422212742195	1.203451992999015
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3995	0.49323606067976444	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCG	1603	0.19791174099365766	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1329	0.1640827846416538	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGC	1298	0.16025542096679204	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTC	1238	0.15284762030576932	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	828	0.10222764912211389	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2346334435037908E-4	0.0	0.0	0.03358202966330311	0.0
2	1.2346334435037908E-4	0.0	0.0	0.17037941520352315	0.0
3	1.2346334435037908E-4	0.0	0.0	0.2647054102872128	0.0
4	1.2346334435037908E-4	0.0	0.0	0.3945888485438116	0.0
5	1.2346334435037908E-4	0.0	0.0	0.8757255014772389	0.0
6	1.2346334435037908E-4	0.0	0.0	1.1843838623531866	0.0
7	1.2346334435037908E-4	0.0	0.0	1.4869925193559659	0.0
8	1.2346334435037908E-4	0.0	0.0	1.9813397501348837	0.0
9	1.2346334435037908E-4	0.0	0.0	2.2241921484720795	0.0
10	1.2346334435037908E-4	0.0	0.0	2.9073148327627267	0.0
11	1.2346334435037908E-4	0.0	0.0	3.397093919800681	0.0
12	1.2346334435037908E-4	0.0	0.0	4.009595571122912	0.0
13	1.2346334435037908E-4	0.0	0.0	4.2108408224140295	0.0
14	1.2346334435037908E-4	0.0	0.0	4.311216521370888	0.0
15	1.2346334435037908E-4	0.0	0.0	4.4648049217427594	0.0
16	1.2346334435037908E-4	0.0	0.0	4.7058053699146996	0.0
17	1.2346334435037908E-4	0.0	0.0	5.009772123705332	0.0
18	1.2346334435037908E-4	0.0	0.0	5.336949986233837	0.0
19	1.2346334435037908E-4	0.0	0.0	5.541034894445014	0.0
20	1.2346334435037908E-4	0.0	0.0	5.755367260237272	0.0
21	1.2346334435037908E-4	0.0	0.0	6.01846764704793	0.0
22	1.2346334435037908E-4	0.0	0.0	6.297247878591086	0.0
23	1.2346334435037908E-4	0.0	0.0	6.578126986988198	0.0
24	1.2346334435037908E-4	0.0	0.0	6.811225781121714	0.0
25	1.2346334435037908E-4	0.0	0.0	7.017039176153796	0.0
26	1.2346334435037908E-4	0.0	0.0	7.233964272177412	0.0
27	1.2346334435037908E-4	0.0	0.0	7.434839133435479	0.0
28	1.2346334435037908E-4	0.0	0.0	7.659048566775767	0.0
29	1.2346334435037908E-4	0.0	0.0	7.8895546306779245	0.0
30	1.2346334435037908E-4	0.0	0.0	8.17463149278295	0.0
31	2.4692668870075817E-4	0.0	0.0	8.402421363109399	0.0
32	2.4692668870075817E-4	0.0	0.0	8.647743028333602	0.0
33	2.4692668870075817E-4	0.0	0.0	8.877508312169658	0.0
34	3.703900330511373E-4	0.0	0.0	9.12221266067211	0.0
35	3.703900330511373E-4	0.0	0.0	9.390498507945484	0.0
36	3.703900330511373E-4	0.0	0.0	9.662117865516317	0.0
37	3.703900330511373E-4	0.0	0.0	9.92682327580353	0.0
38	3.703900330511373E-4	0.0	0.0	10.208566627611095	0.0
39	3.703900330511373E-4	0.0	0.0	10.517595378520094	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTC	25	3.8897815E-5	45.0	18
CGTTGCT	20	7.032051E-4	45.0	16
CGAGCAT	35	1.2113378E-7	45.0	22
CGGTCTA	45	3.8380676E-10	45.0	31
TATCGCG	20	7.032051E-4	45.0	1
TAATGCG	25	3.8897815E-5	45.0	1
CGTTATT	350	0.0	42.42857	1
CGTGCGG	55	6.002665E-11	40.909092	2
CGTTTTT	1675	0.0	40.701492	1
GTTAGCG	45	1.9270374E-8	40.0	1
TATTACG	45	1.9270374E-8	40.0	1
GCGAGAC	220	0.0	38.863636	21
CCCGATC	35	6.246006E-6	38.571426	41
CACAACG	300	0.0	38.250004	12
CGGTTTT	470	0.0	37.81915	1
CGTTTTA	215	0.0	37.67442	1
TCGCTAT	30	1.1396242E-4	37.500004	23
TCGAACG	30	1.1396242E-4	37.500004	1
ACGGGTC	90	0.0	37.5	5
CGCACGG	85	0.0	37.058823	2
>>END_MODULE