FastQCFastQC Report
Sat 18 Jun 2016
SRR3551067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526330
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5650.10734710162825604No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC5570.10582714266714797TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG207.0297957E-445.0000041
GTCGATC207.0297957E-445.00000440
CGATTGT302.1632513E-645.00000410
TCGATTG207.0297957E-445.0000041
AAGCCGT207.0297957E-445.00000428
CGGTCTA302.1632513E-645.00000431
ATAACGG302.1632513E-645.0000042
AAAGCGT207.0297957E-445.00000418
TGCGTAG207.0297957E-445.0000042
TTCACGG253.887913E-545.02
TAAACCG253.887913E-545.09
ATTGTCG253.887913E-545.038
TCGTAAG351.2102464E-745.01
GACGAAC253.887913E-545.010
AGTACGG603.6379788E-1241.2500042
AGGCGAT1100.040.9090927
GCGATAT451.9248546E-840.09
TCCAATA403.4534605E-739.37500411
CGAAGCC403.4534605E-739.37500417
CGAGGTT356.2418367E-638.57142635