##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551067_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	526330
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.889022096403398	31.0	31.0	34.0	30.0	34.0
2	32.02204320483347	33.0	31.0	34.0	30.0	34.0
3	31.97717591625026	33.0	31.0	34.0	30.0	34.0
4	35.7054927516957	37.0	35.0	37.0	35.0	37.0
5	31.11506279330458	37.0	35.0	37.0	0.0	37.0
6	33.36807326202192	37.0	35.0	37.0	17.0	37.0
7	29.629441605076664	35.0	32.0	37.0	0.0	37.0
8	32.64489958771113	35.0	33.0	37.0	17.0	37.0
9	36.4314156517774	39.0	35.0	39.0	32.0	39.0
10	36.78831531548648	38.0	35.0	39.0	33.0	39.0
11	36.96052856572872	39.0	37.0	39.0	33.0	39.0
12	36.952159291699125	39.0	37.0	39.0	33.0	39.0
13	36.74328083141755	39.0	35.0	39.0	33.0	39.0
14	37.97752170691391	40.0	37.0	41.0	33.0	41.0
15	38.08564018771493	40.0	37.0	41.0	33.0	41.0
16	38.12975889650979	40.0	37.0	41.0	33.0	41.0
17	38.11212737256094	40.0	37.0	41.0	33.0	41.0
18	38.03176904223586	40.0	37.0	41.0	33.0	41.0
19	37.94950506336329	40.0	37.0	41.0	33.0	41.0
20	37.88688085421693	40.0	36.0	41.0	33.0	41.0
21	37.79277259513993	40.0	36.0	41.0	33.0	41.0
22	37.77056029487204	40.0	36.0	41.0	33.0	41.0
23	37.72961260045979	39.0	36.0	41.0	33.0	41.0
24	37.691543328330134	40.0	36.0	41.0	33.0	41.0
25	37.54533657591245	39.0	36.0	41.0	33.0	41.0
26	37.38384093629472	39.0	36.0	41.0	32.0	41.0
27	37.326960272072654	39.0	36.0	41.0	32.0	41.0
28	37.21886649060475	39.0	35.0	41.0	32.0	41.0
29	37.16575722455494	39.0	35.0	41.0	32.0	41.0
30	37.0056257481048	39.0	35.0	41.0	31.0	41.0
31	36.887260843957215	39.0	35.0	41.0	31.0	41.0
32	36.74760891455931	39.0	35.0	41.0	31.0	41.0
33	36.62038644956586	39.0	35.0	41.0	30.0	41.0
34	36.521655615298386	39.0	35.0	40.0	30.0	41.0
35	36.370847187125946	39.0	35.0	41.0	30.0	41.0
36	36.23448976877624	39.0	35.0	40.0	30.0	41.0
37	36.10421028632227	39.0	35.0	40.0	29.0	41.0
38	35.99584481219007	39.0	35.0	40.0	29.0	41.0
39	36.04122888682006	39.0	35.0	40.0	29.0	41.0
40	35.897645963558986	39.0	35.0	40.0	28.0	41.0
41	35.76098645336576	38.0	35.0	40.0	27.0	41.0
42	35.67084718712595	38.0	35.0	40.0	27.0	41.0
43	35.491324834229474	38.0	34.0	40.0	26.0	41.0
44	35.338795052533584	38.0	34.0	40.0	26.0	41.0
45	35.2216727148367	38.0	34.0	40.0	26.0	41.0
46	35.093141185188	38.0	34.0	40.0	25.0	41.0
47	34.91447190925845	38.0	34.0	40.0	24.0	41.0
48	34.78701575057473	38.0	34.0	40.0	23.0	41.0
49	34.6327855147911	38.0	34.0	40.0	24.0	41.0
50	34.45877491307735	37.0	33.0	40.0	23.0	41.0
51	32.394887238044575	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	26.0
10	25.0
11	15.0
12	18.0
13	11.0
14	18.0
15	30.0
16	40.0
17	104.0
18	242.0
19	425.0
20	680.0
21	1221.0
22	1742.0
23	2676.0
24	3587.0
25	4681.0
26	5754.0
27	6875.0
28	7963.0
29	9655.0
30	11563.0
31	14674.0
32	19270.0
33	26627.0
34	38314.0
35	45810.0
36	51950.0
37	79795.0
38	98822.0
39	93700.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.55119411775882	19.512093173484317	25.739745026884275	11.19696768187259
2	30.82286778256987	25.558299925902002	25.497881557197953	18.120950734330172
3	32.50869226530884	24.93777668002964	26.277620504246386	16.275910550415137
4	29.079474854178937	26.49763456386677	25.63315790473657	18.789732677217717
5	22.363726179393158	39.749776756027586	20.840537305492752	17.045959759086504
6	26.108335074952976	36.514544107309106	23.323960253073167	14.053160564664754
7	69.47200425588508	19.69866813596033	6.572492542701347	4.256835065453233
8	82.53092166511505	6.026637280793419	7.214865198639636	4.227575855451902
9	75.61320844337203	8.93906864514658	9.715197689662379	5.732525221819011
10	40.0182395075333	29.018866490604754	17.417779719947564	13.545114281914389
11	31.65865521630916	26.38040772899132	23.911804381281705	18.049132673417816
12	29.441415081792794	23.77234814660004	28.167879467254387	18.61835730435278
13	25.00237493587673	25.177170216404154	29.50525335815933	20.31520148955978
14	20.121786711758784	29.354207436399214	28.571998556038984	21.952007295803014
15	19.426975471662264	27.683582543271328	33.65702126042597	19.232420724640434
16	21.758592518002015	26.637090798548442	31.64953546254251	19.954781220907037
17	22.069994110159026	25.390154465829422	28.39359337297893	24.14625805103262
18	22.98652936370718	27.334751961697034	29.09543442327057	20.583284251325214
19	25.227708851860996	27.744000911975377	26.850835027454256	20.177455208709365
20	27.44912887352042	27.068949138373267	26.880664222066002	18.601257766040316
21	25.609218551099122	27.561795831512548	26.904983565443736	19.924002051944598
22	22.923831056561472	25.838922349096578	27.573385518591	23.663861075750955
23	22.293998062052324	28.06015237588585	28.480421028632225	21.165428533429598
24	22.99925902000646	25.92973989702278	29.12089373586913	21.95010734710163
25	22.166131514449113	27.539946421446622	27.318792392605403	22.97512967149887
26	21.418881690194365	28.615697376170846	27.815628978017592	22.1497919556172
27	22.58754013641632	28.053312560560865	28.244447399920205	21.114699903102615
28	20.518685995478123	28.454581726293387	29.818364904147586	21.2083673740809
29	22.305587749130773	27.122147702012043	28.736724108449074	21.835540440408106
30	22.47753310660612	26.54437330192085	28.669655919290175	22.30843767218285
31	23.885015104592174	27.505937339691826	26.043356829365607	22.565690726350386
32	23.381718693595275	28.497140577204416	27.061159348697583	21.059981380502727
33	22.5208519369977	28.323295271027682	26.8179659149203	22.33788687705432
34	21.928067942165562	27.493017688522407	27.87680732620219	22.702107043109837
35	22.81762392415405	26.088005623848154	27.94843539224441	23.145935059753388
36	23.236942602549732	28.75344365702126	27.116827845648167	20.89278589478084
37	22.633518894989834	28.97497767560276	27.756350578534377	20.635152850873027
38	21.77170216404157	28.60999753006669	26.20067258184029	23.417627724051453
39	22.27309862633709	27.304352782474872	27.254764121368723	23.167784469819313
40	22.784944806490227	25.17926015997568	29.268899739707027	22.766895293827066
41	20.468717344631695	27.020500446487944	28.689415385784585	23.821366823095776
42	21.843330230083787	27.37959075104972	28.337734881158212	22.439344137708282
43	22.430034389071494	26.532973609712542	28.3668040962894	22.670187904926568
44	21.599186821955808	26.93424277544506	27.280223433967283	24.18634696863185
45	21.342883742138962	27.18218608097581	26.752227689852376	24.722702487032848
46	22.31831740543005	27.29352307487698	26.664830049588662	23.723329470104307
47	22.015085592688997	26.26413086846655	28.866680599623812	22.854102939220642
48	20.971443771018183	25.953109266049818	29.446164953546255	23.629282009385747
49	20.91444530997663	25.73005528850721	29.63976972621739	23.715729675298768
50	19.39201641555678	26.39427735451143	29.55085212699257	24.66285410293922
51	20.19683468546349	25.601048771683168	27.933235802633327	26.268880740220013
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	257.0
1	640.0
2	1023.0
3	1415.5
4	1808.0
5	1492.5
6	1177.0
7	1018.0
8	859.0
9	866.0
10	873.0
11	871.5
12	870.0
13	923.5
14	977.0
15	958.0
16	939.0
17	1068.0
18	1197.0
19	1329.5
20	1462.0
21	2084.5
22	2707.0
23	2934.0
24	3161.0
25	3669.5
26	5257.5
27	6337.0
28	7370.0
29	8403.0
30	9647.5
31	10892.0
32	12544.0
33	14196.0
34	15525.5
35	16855.0
36	18260.0
37	19665.0
38	20888.5
39	22112.0
40	24574.0
41	27036.0
42	29870.5
43	32705.0
44	36086.0
45	39467.0
46	42594.0
47	45721.0
48	45407.5
49	45094.0
50	42926.0
51	40758.0
52	37093.5
53	33429.0
54	30481.0
55	27533.0
56	26154.5
57	24776.0
58	23913.5
59	23051.0
60	22080.0
61	21109.0
62	18677.5
63	16246.0
64	13148.5
65	10051.0
66	8434.0
67	6817.0
68	5678.5
69	4540.0
70	3800.5
71	3061.0
72	2546.0
73	2031.0
74	1581.5
75	927.0
76	722.0
77	619.5
78	517.0
79	404.0
80	291.0
81	216.0
82	141.0
83	107.0
84	73.0
85	51.5
86	30.0
87	26.5
88	23.0
89	14.5
90	6.0
91	6.0
92	6.0
93	7.0
94	8.0
95	5.5
96	3.0
97	2.5
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	526330.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.120406925980205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.76054751780138	26.642609914260845
2	8.921162440878376	6.444720352345954
3	3.8176159280376813	4.136815224234739
4	2.4692403254337165	3.567598614108225
5	1.67846516204937	3.0313422332152276
6	1.3185981601755328	2.8576981270433444
7	0.9948045761696278	2.5152922272151956
8	0.7750041544778352	2.2394772343251703
9	0.597786531989565	1.9433063511300215
>10	5.513846420436527	41.711345229882106
>50	0.12569488976745938	2.854619668948811
>100	0.026186435368211955	1.8429227842870524
>500	0.0010474574147284782	0.2122520390032922
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	565	0.10734710162825604	No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	557	0.10582714266714797	TruSeq Adapter, Index 16 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10335720935534741	0.0
2	0.0	0.0	0.0	0.5065263237892577	0.0
3	0.0	0.0	0.0	0.6832215530180685	0.0
4	0.0	0.0	0.0	0.9269849714057721	0.0
5	0.0	0.0	0.0	1.7129937491687723	0.0
6	0.0	0.0	0.0	2.175821252826174	0.0
7	0.0	0.0	0.0	2.661258146030057	0.0
8	0.0	0.0	0.0	3.545494271654665	0.0
9	0.0	0.0	0.0	3.875325366215112	0.0
10	0.0	0.0	0.0	4.760701461060552	0.0
11	0.0	0.0	0.0	5.78990367260084	0.0
12	0.0	0.0	0.0	6.636330819067886	0.0
13	0.0	0.0	0.0	6.97281173408318	0.0
14	0.0	0.0	0.0	7.120627743050938	0.0
15	0.0	0.0	0.0	7.396880284232326	0.0
16	0.0	0.0	0.0	7.9856743867915565	0.0
17	0.0	0.0	0.0	8.662436114224915	0.0
18	0.0	0.0	0.0	9.440845097182375	0.0
19	0.0	0.0	0.0	9.898732734216177	0.0
20	0.0	0.0	0.0	10.34598065852222	0.0
21	0.0	0.0	0.0	10.882146182053084	0.0
22	0.0	0.0	0.0	11.467140387209545	0.0
23	0.0	0.0	0.0	12.06562422814584	0.0
24	0.0	0.0	0.0	12.512492162711606	0.0
25	0.0	0.0	0.0	12.903311610586513	0.0
26	0.0	0.0	0.0	13.280641422681587	0.0
27	0.0	0.0	0.0	13.657401250166245	0.0
28	0.0	0.0	0.0	14.071210077327912	0.0
29	0.0	0.0	0.0	14.486348868580547	0.0
30	0.0	0.0	0.0	14.944426500484488	0.0
31	0.0	0.0	0.0	15.38407462998499	0.0
32	0.0	0.0	0.0	15.813843026238292	0.0
33	0.0	0.0	0.0	16.22860182775065	0.0
34	0.0	0.0	0.0	16.663310090627554	0.0
35	0.0	0.0	0.0	17.110938004673873	0.0
36	0.0	0.0	0.0	17.555905990538257	0.0
37	0.0	0.0	0.0	17.992324207246405	0.0
38	0.0	0.0	0.0	18.435962229019818	0.0
39	0.0	0.0	0.0	18.87048049702658	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.0297957E-4	45.000004	1
GTCGATC	20	7.0297957E-4	45.000004	40
CGATTGT	30	2.1632513E-6	45.000004	10
TCGATTG	20	7.0297957E-4	45.000004	1
AAGCCGT	20	7.0297957E-4	45.000004	28
CGGTCTA	30	2.1632513E-6	45.000004	31
ATAACGG	30	2.1632513E-6	45.000004	2
AAAGCGT	20	7.0297957E-4	45.000004	18
TGCGTAG	20	7.0297957E-4	45.000004	2
TTCACGG	25	3.887913E-5	45.0	2
TAAACCG	25	3.887913E-5	45.0	9
ATTGTCG	25	3.887913E-5	45.0	38
TCGTAAG	35	1.2102464E-7	45.0	1
GACGAAC	25	3.887913E-5	45.0	10
AGTACGG	60	3.6379788E-12	41.250004	2
AGGCGAT	110	0.0	40.909092	7
GCGATAT	45	1.9248546E-8	40.0	9
TCCAATA	40	3.4534605E-7	39.375004	11
CGAAGCC	40	3.4534605E-7	39.375004	17
CGAGGTT	35	6.2418367E-6	38.571426	35
>>END_MODULE