FastQCFastQC Report
Sat 18 Jun 2016
SRR3551065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347879
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG10770.30959040355985845No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC9930.28544407681981376No Hit
GAATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTC6420.18454692579891283No Hit
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC5440.15637621126886073TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5240.15062708585456439No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4180.12015672115879372No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3600.1034842574573343No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3500.10060969475018612No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAGC253.8851773E-545.00000427
CAAACGG253.8851773E-545.0000042
ATTAGGT207.0264953E-445.0000044
CAACGTT253.8851773E-545.00000439
TTTCGTC207.0264953E-445.00000415
CGGTTGC207.0264953E-445.00000445
TTATGAG253.8851773E-545.0000041
TAAACGG207.0264953E-445.0000042
CAATTGC207.0264953E-445.00000436
TTCCGAG207.0264953E-445.00000415
CGATTAC302.1611213E-645.00000410
TCGATTG207.0264953E-445.0000041
TCGATGG302.1611213E-645.0000042
TGACGAA207.0264953E-445.0000041
TCTAGGC207.0264953E-445.00000430
AACGGCT253.8851773E-545.0000047
CGGGCAC406.7921064E-945.0000046
GATATGT207.0264953E-445.0000049
CGAATGT207.0264953E-445.00000444
CGAATAT502.1827873E-1145.00000414