##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551065_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347879
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76046268961334	31.0	31.0	34.0	30.0	34.0
2	31.898159992411156	31.0	31.0	34.0	30.0	34.0
3	31.840194435421513	33.0	31.0	34.0	30.0	34.0
4	35.59483038642747	37.0	35.0	37.0	33.0	37.0
5	31.011877693105937	37.0	35.0	37.0	0.0	37.0
6	33.271350095866666	37.0	35.0	37.0	17.0	37.0
7	29.62786486105801	35.0	32.0	37.0	0.0	37.0
8	32.589992497391336	35.0	33.0	37.0	17.0	37.0
9	36.32970659338448	39.0	35.0	39.0	32.0	39.0
10	36.70002788325826	38.0	35.0	39.0	33.0	39.0
11	36.844135460893014	39.0	37.0	39.0	33.0	39.0
12	36.825706639377486	39.0	35.0	39.0	33.0	39.0
13	36.50374124336335	39.0	35.0	39.0	32.0	39.0
14	37.707973174580815	40.0	36.0	41.0	33.0	41.0
15	37.83062214160671	40.0	36.0	41.0	33.0	41.0
16	37.915740817928075	40.0	36.0	41.0	33.0	41.0
17	37.86956384260044	40.0	36.0	41.0	33.0	41.0
18	37.79766815473196	40.0	36.0	41.0	33.0	41.0
19	37.72343544738257	39.0	36.0	41.0	33.0	41.0
20	37.652948295240584	39.0	36.0	41.0	33.0	41.0
21	37.55607553200969	39.0	36.0	41.0	33.0	41.0
22	37.526878598593186	39.0	36.0	41.0	33.0	41.0
23	37.48832496356492	39.0	35.0	41.0	33.0	41.0
24	37.42373066497259	39.0	35.0	41.0	32.0	41.0
25	37.32422480230195	39.0	35.0	41.0	32.0	41.0
26	37.14293188148753	39.0	35.0	41.0	32.0	41.0
27	37.12364931484798	39.0	35.0	41.0	32.0	41.0
28	36.992764725666106	39.0	35.0	41.0	31.0	41.0
29	36.97548572923344	39.0	35.0	41.0	31.0	41.0
30	36.888903900494135	39.0	35.0	40.0	31.0	41.0
31	36.74458073065635	39.0	35.0	40.0	31.0	41.0
32	36.698280149132316	39.0	35.0	40.0	31.0	41.0
33	36.541699843911246	39.0	35.0	40.0	30.0	41.0
34	36.39035986650531	39.0	35.0	40.0	30.0	41.0
35	36.32991931102481	39.0	35.0	40.0	30.0	41.0
36	36.172399023798505	38.0	35.0	40.0	30.0	41.0
37	36.03117463255902	38.0	35.0	40.0	29.0	41.0
38	35.933356138197475	38.0	35.0	40.0	29.0	41.0
39	35.8811253338086	38.0	35.0	40.0	29.0	41.0
40	35.67918730363144	38.0	35.0	40.0	27.0	41.0
41	35.54963938610839	38.0	35.0	40.0	27.0	41.0
42	35.46453220803785	38.0	34.0	40.0	27.0	41.0
43	35.25290977610031	38.0	34.0	40.0	26.0	41.0
44	34.99500401001497	38.0	34.0	40.0	24.0	41.0
45	34.88790642723475	38.0	34.0	40.0	24.0	41.0
46	34.683746360084974	38.0	34.0	40.0	23.0	41.0
47	34.46935284969774	37.0	33.0	40.0	23.0	41.0
48	34.3807875726905	37.0	33.0	40.0	23.0	41.0
49	34.17326714173606	37.0	33.0	40.0	22.0	41.0
50	33.99270723441197	37.0	33.0	40.0	22.0	41.0
51	31.83516682524671	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	10.0
10	17.0
11	8.0
12	2.0
13	5.0
14	10.0
15	22.0
16	24.0
17	100.0
18	173.0
19	305.0
20	521.0
21	801.0
22	1133.0
23	1692.0
24	2465.0
25	3233.0
26	4000.0
27	4822.0
28	5817.0
29	6934.0
30	8680.0
31	10918.0
32	14012.0
33	19421.0
34	26955.0
35	32335.0
36	34608.0
37	51080.0
38	61894.0
39	55871.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.87295295203217	20.562321956772326	26.024278556624576	10.540446534570929
2	30.963064743775853	27.14363327478807	25.331796400472577	16.561505580963495
3	32.403220660057094	26.389635476703106	25.37031554074836	15.836828322491442
4	28.260113430244427	25.940053869305128	26.560384501507706	19.239448198942736
5	22.389394013435705	40.49856415592778	21.155919155798426	15.956122674838092
6	24.877328036472452	37.4785485757979	23.46649265980413	14.177630727925514
7	67.53440132919779	20.006668985480584	8.0237668844627	4.43516280085892
8	79.58313091620937	7.403723708530839	8.13529991750005	4.877845457759738
9	72.72931105355597	9.525438442676908	9.933913803362664	7.811336700404451
10	39.78940953607433	29.61604465920622	16.822515874772552	13.772029929946909
11	30.608056249443056	26.36893862521164	25.079984707326396	17.94302041801891
12	29.333762601364267	23.730090060049616	28.72291802609528	18.213229312490835
13	23.62430615242656	28.07844106715266	30.632202576183097	17.66505020423768
14	18.528568841464992	31.361766591257307	29.11615820443315	20.993506362844553
15	17.930372342107457	27.567056361551	35.784281316204776	18.71828998013677
16	20.44676453594497	25.925681055769388	32.86861236234438	20.75894204594126
17	19.80171266446092	25.405097749504858	29.96587894066615	24.827310645368073
18	21.130623003975522	26.595454166534914	30.45254240698634	21.821380422503225
19	23.50069995601919	28.016063056407543	27.139033974456634	21.34420301311663
20	25.520080257790784	27.643519729561138	28.17387654902998	18.662523463618097
21	23.36990735284395	28.644729920460847	27.5825790001696	20.402783726525602
22	21.0972780765726	26.73170843885374	27.254591395283995	24.916422089289668
23	20.393297669592016	29.390966399236518	27.64840648616329	22.567329445008177
24	21.787173126288163	26.342492648305875	29.859232664230955	22.011101561175007
25	19.943428605923323	29.41626255105942	27.835253062127922	22.805055780889333
26	19.600493274960545	30.24988573613239	27.416429275696437	22.733191713210626
27	21.180927851350614	29.66404985641559	27.09102877724726	22.06399351498653
28	18.348621215997515	29.574650956223287	30.63306494499524	21.443662882783958
29	18.910885681515698	28.900278545126323	28.625470350322956	23.56336542303502
30	20.213350044124535	28.46880668278338	27.77891163306782	23.53893164002426
31	22.58773884022893	29.440983790340898	25.28724067851178	22.684036690918393
32	21.679089568499393	29.468867048600234	25.12367806047505	23.728365322425326
33	21.666728948858655	27.715958709781276	25.581020987182324	25.03629135417775
34	18.85885609651632	28.8416374659005	28.79162007479612	23.507886362787062
35	20.271128754538214	26.566133626922007	27.928676350110237	25.234061268429542
36	22.356336542303502	28.26557509938801	27.038999192247882	22.339089166060614
37	20.317121757852586	29.016985791036536	27.937012581960968	22.728879869149907
38	19.94141641202832	29.044869049295873	26.36663897504592	24.647075563629883
39	22.003340241865708	26.152484053363384	28.356698737204606	23.48747696756631
40	21.726519853167336	25.513181307293625	30.543953501073652	22.216345338465384
41	19.23801091758916	26.247057166428554	30.02337019480911	24.491561721173166
42	20.652870682047496	26.26401708640073	29.50882347022959	23.574288761322183
43	22.587451383958214	25.51979280152007	27.581716631357455	24.31103918316426
44	20.4959195582372	26.97575881269062	27.19595031605817	25.332371313014008
45	19.64131206540205	27.386246367271376	26.789487149267416	26.18295441805915
46	21.938662580954873	27.991629273396786	26.907919132801922	23.16178901284642
47	19.810911265123792	26.67996631012507	30.96565185021229	22.543470574538848
48	21.053872179694665	25.723886753727587	28.7505138280839	24.47172723849384
49	20.689952540969706	24.494436283880315	30.21682826500019	24.598782910149794
50	18.661661094805954	26.101316837176146	30.128866646161452	25.10815542185645
51	18.704204622871746	25.86416541383642	27.601838570307493	27.829791392984344
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	302.0
1	582.0
2	862.0
3	1168.0
4	1474.0
5	1165.0
6	856.0
7	771.0
8	686.0
9	692.0
10	698.0
11	770.0
12	842.0
13	879.0
14	916.0
15	964.5
16	1013.0
17	1020.0
18	1027.0
19	1232.5
20	1438.0
21	1515.5
22	1593.0
23	1879.0
24	2165.0
25	2666.0
26	3869.5
27	4572.0
28	5283.0
29	5994.0
30	6713.0
31	7432.0
32	8257.5
33	9083.0
34	10032.0
35	10981.0
36	11135.0
37	11289.0
38	13009.5
39	14730.0
40	16825.0
41	18920.0
42	21513.0
43	24106.0
44	26577.5
45	29049.0
46	31787.5
47	34526.0
48	33138.0
49	31750.0
50	30348.5
51	28947.0
52	24778.0
53	20609.0
54	18259.0
55	15909.0
56	14703.0
57	13497.0
58	12592.0
59	11687.0
60	11296.5
61	10906.0
62	9767.5
63	8629.0
64	7169.5
65	5710.0
66	4817.5
67	3925.0
68	3336.5
69	2748.0
70	2401.0
71	2054.0
72	1734.0
73	1414.0
74	1204.0
75	797.0
76	600.0
77	453.5
78	307.0
79	234.5
80	162.0
81	141.5
82	121.0
83	113.5
84	106.0
85	72.0
86	38.0
87	32.0
88	26.0
89	18.0
90	10.0
91	7.5
92	5.0
93	4.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	347879.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.853966925052305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.65577302462228	29.75328708887508
2	9.473687744329691	7.551280760415777
3	3.8927769915900794	4.654278164083068
4	2.4209211931741903	3.8593325264368934
5	1.6299057345237407	3.247910461733112
6	1.178625593073685	2.818374324202728
7	0.7972691417434915	2.2242036603786963
8	0.6301657352973122	2.009168349747228
9	0.5218067849173939	1.8716463312629124
>10	4.682880547626512	36.337971425057596
>50	0.07649612400480056	1.9856646479490445
>100	0.036083077360754987	2.599713608460518
>500	0.0028866461888603983	0.7774118689754154
>1k	7.216615472150996E-4	0.3097567824219469
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG	1077	0.30959040355985845	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC	993	0.28544407681981376	No Hit
GAATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTC	642	0.18454692579891283	No Hit
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	544	0.15637621126886073	TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	524	0.15062708585456439	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	418	0.12015672115879372	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	360	0.1034842574573343	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	350	0.10060969475018612	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.74912541429635E-4	0.0	0.0	0.14574032925241248	0.0
2	5.74912541429635E-4	0.0	0.0	0.691332331069136	0.0
3	5.74912541429635E-4	0.0	0.0	0.9109489218952567	0.0
4	5.74912541429635E-4	0.0	0.0	1.2533093403166042	0.0
5	5.74912541429635E-4	0.0	0.0	2.408308636048741	0.0
6	5.74912541429635E-4	0.0	0.0	2.92975431112542	0.0
7	5.74912541429635E-4	0.0	0.0	3.58371732700163	0.0
8	5.74912541429635E-4	0.0	0.0	4.682375193673662	0.0
9	5.74912541429635E-4	0.0	0.0	5.107810474331592	0.0
10	5.74912541429635E-4	0.0	0.0	6.526694626579932	0.0
11	5.74912541429635E-4	0.0	0.0	7.755282727615062	0.0
12	5.74912541429635E-4	0.0	0.0	9.152895115830503	0.0
13	5.74912541429635E-4	0.0	0.0	9.561083020245546	0.0
14	5.74912541429635E-4	0.0	0.0	9.742467927066595	0.0
15	5.74912541429635E-4	0.0	0.0	10.14548161860877	0.0
16	5.74912541429635E-4	0.0	0.0	10.832214649346469	0.0
17	5.74912541429635E-4	0.0	0.0	11.684522492015901	0.0
18	5.74912541429635E-4	0.0	0.0	12.646926086369112	0.0
19	5.74912541429635E-4	0.0	0.0	13.153711491639335	0.0
20	5.74912541429635E-4	0.0	0.0	13.694991649395336	0.0
21	5.74912541429635E-4	0.0	0.0	14.313310087702908	0.0
22	5.74912541429635E-4	0.0	0.0	14.948588445982654	0.0
23	5.74912541429635E-4	0.0	0.0	15.630722176388916	0.0
24	5.74912541429635E-4	0.0	0.0	16.182063303619937	0.0
25	5.74912541429635E-4	0.0	0.0	16.667289488586547	0.0
26	5.74912541429635E-4	0.0	0.0	17.126644609188826	0.0
27	5.74912541429635E-4	0.0	0.0	17.552079889846755	0.0
28	5.74912541429635E-4	0.0	0.0	18.01545939823904	0.0
29	5.74912541429635E-4	0.0	0.0	18.46647828699059	0.0
30	5.74912541429635E-4	0.0	0.0	19.01005809491231	0.0
31	5.74912541429635E-4	0.0	0.0	19.50333305545894	0.0
32	5.74912541429635E-4	0.0	0.0	20.02909057459634	0.0
33	5.74912541429635E-4	0.0	0.0	20.515179128375095	0.0
34	5.74912541429635E-4	0.0	0.0	20.997818206905276	0.0
35	5.74912541429635E-4	0.0	0.0	21.5201262507941	0.0
36	5.74912541429635E-4	0.0	0.0	21.98810505951782	0.0
37	5.74912541429635E-4	0.0	0.0	22.459820799760838	0.0
38	5.74912541429635E-4	0.0	0.0	22.9111271447831	0.0
39	5.74912541429635E-4	0.0	0.0	23.39980280499829	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTAGC	25	3.8851773E-5	45.000004	27
CAAACGG	25	3.8851773E-5	45.000004	2
ATTAGGT	20	7.0264953E-4	45.000004	4
CAACGTT	25	3.8851773E-5	45.000004	39
TTTCGTC	20	7.0264953E-4	45.000004	15
CGGTTGC	20	7.0264953E-4	45.000004	45
TTATGAG	25	3.8851773E-5	45.000004	1
TAAACGG	20	7.0264953E-4	45.000004	2
CAATTGC	20	7.0264953E-4	45.000004	36
TTCCGAG	20	7.0264953E-4	45.000004	15
CGATTAC	30	2.1611213E-6	45.000004	10
TCGATTG	20	7.0264953E-4	45.000004	1
TCGATGG	30	2.1611213E-6	45.000004	2
TGACGAA	20	7.0264953E-4	45.000004	1
TCTAGGC	20	7.0264953E-4	45.000004	30
AACGGCT	25	3.8851773E-5	45.000004	7
CGGGCAC	40	6.7921064E-9	45.000004	6
GATATGT	20	7.0264953E-4	45.000004	9
CGAATGT	20	7.0264953E-4	45.000004	44
CGAATAT	50	2.1827873E-11	45.000004	14
>>END_MODULE