FastQCFastQC Report
Sat 18 Jun 2016
SRR3551064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904856
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63140.6977905876736188No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20630.22799207829754128No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12750.14090639836614888No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC207.0324907E-445.014
CCTACGC253.890146E-545.034
CAATACG253.890146E-545.01
CGGTTTA600.044.9999961
CGTTTTT26550.041.440681
AAGTACG556.002665E-1140.9090921
CGTTATT5650.040.221241
CGGTTTT5550.039.3243261
CTTACGG700.038.5714262
TACGGGA4850.038.5051574
CGTTTTA3350.037.611941
ATTACGG900.037.52
TTCACGG601.546141E-1037.4999962
GTCAACG301.1397301E-437.4999961
TTGTACG552.748493E-936.818181
CGGGATC2350.036.382986
AGGCGAT1800.036.257
TTACGGG3600.036.253
ACGGGAT5800.036.0775875
ACGCACG250.002106872436.01
Produced by FastQC (version 0.11.5)