##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551064_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	904856
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85183388296038	31.0	31.0	34.0	30.0	34.0
2	31.970738990513407	33.0	31.0	34.0	30.0	34.0
3	31.67768131061738	33.0	31.0	34.0	30.0	34.0
4	35.53823481305313	37.0	35.0	37.0	33.0	37.0
5	31.000179034012042	37.0	35.0	37.0	0.0	37.0
6	33.31245634664521	37.0	35.0	37.0	17.0	37.0
7	29.71377324126712	35.0	32.0	37.0	0.0	37.0
8	32.737954989523196	35.0	35.0	37.0	17.0	37.0
9	36.580379640517386	39.0	35.0	39.0	32.0	39.0
10	36.86839231877779	38.0	37.0	39.0	33.0	39.0
11	36.977614117605455	39.0	37.0	39.0	33.0	39.0
12	37.00342485434147	39.0	37.0	39.0	33.0	39.0
13	36.85313574756646	39.0	37.0	39.0	33.0	39.0
14	38.021529392522126	40.0	37.0	41.0	33.0	41.0
15	38.13506790030679	40.0	37.0	41.0	33.0	41.0
16	38.200088190828154	40.0	37.0	41.0	33.0	41.0
17	38.19077400160909	40.0	37.0	41.0	33.0	41.0
18	38.08849032332216	40.0	37.0	41.0	33.0	41.0
19	37.96278965934911	40.0	37.0	41.0	33.0	41.0
20	37.80415005260505	40.0	36.0	41.0	33.0	41.0
21	37.69929800984908	40.0	36.0	41.0	33.0	41.0
22	37.707628617150135	39.0	36.0	41.0	33.0	41.0
23	37.630712511161995	39.0	36.0	41.0	33.0	41.0
24	37.573153076290595	39.0	35.0	41.0	33.0	41.0
25	37.443351207263916	39.0	35.0	41.0	33.0	41.0
26	37.2908142290044	39.0	35.0	41.0	32.0	41.0
27	37.25502842441228	39.0	35.0	41.0	32.0	41.0
28	37.13112031085609	39.0	35.0	41.0	32.0	41.0
29	37.07803783143395	39.0	35.0	41.0	32.0	41.0
30	36.92890913029255	39.0	35.0	41.0	31.0	41.0
31	36.795130937961396	39.0	35.0	41.0	31.0	41.0
32	36.671734508032216	39.0	35.0	41.0	31.0	41.0
33	36.43286556092903	39.0	35.0	41.0	30.0	41.0
34	36.17828914213975	39.0	35.0	40.0	29.0	41.0
35	36.00095705836067	39.0	35.0	40.0	29.0	41.0
36	35.837011635000486	39.0	35.0	40.0	27.0	41.0
37	35.68386461492215	39.0	35.0	40.0	27.0	41.0
38	35.513834245449	39.0	35.0	40.0	26.0	41.0
39	35.469024905620344	39.0	35.0	40.0	25.0	41.0
40	35.34138028592395	38.0	35.0	40.0	25.0	41.0
41	35.21003452483047	38.0	34.0	40.0	24.0	41.0
42	35.14611164649403	38.0	34.0	40.0	24.0	41.0
43	35.01705464736931	38.0	34.0	40.0	23.0	41.0
44	34.8606397039971	38.0	34.0	40.0	23.0	41.0
45	34.74092562794522	38.0	34.0	40.0	23.0	41.0
46	34.67574177548693	38.0	34.0	40.0	23.0	41.0
47	34.51139628847021	38.0	34.0	40.0	23.0	41.0
48	34.43511895815467	38.0	33.0	40.0	22.0	41.0
49	34.2993050827977	38.0	33.0	40.0	22.0	41.0
50	34.11034573456992	37.0	33.0	40.0	21.0	41.0
51	32.24478480553812	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	33.0
10	42.0
11	49.0
12	27.0
13	20.0
14	42.0
15	72.0
16	143.0
17	265.0
18	492.0
19	835.0
20	1427.0
21	2409.0
22	3549.0
23	5339.0
24	8086.0
25	11408.0
26	13945.0
27	15601.0
28	16236.0
29	17444.0
30	19947.0
31	24361.0
32	31085.0
33	43200.0
34	60944.0
35	73283.0
36	87292.0
37	135276.0
38	170028.0
39	161948.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.096373345593115	20.683291042994686	24.854120434632694	13.366215176779509
2	35.147470978807675	23.506944751430062	24.936453977207425	16.40913029255484
3	31.876895329201556	23.59314631278347	28.292126039944478	16.2378323180705
4	27.271411141662323	25.521519446188123	28.816629386333293	18.39044002581626
5	21.192653858735532	39.096386607371784	24.011665944636494	15.699293589256191
6	24.84605285260859	34.29904868841009	27.29837675829082	13.556521700690496
7	68.99075654026718	18.825757910650974	8.75951532619555	3.423970222886293
8	83.25490464781137	4.580397322888946	9.234729061861776	2.929968967437913
9	77.40590768033809	7.023659013146844	11.113702069721592	4.456731236793479
10	39.81793788182871	28.405845792037628	18.618432104113804	13.157784222019858
11	29.101205053621793	25.740338794239083	27.588699196336215	17.569756955802912
12	26.447744171448274	23.67183286622402	31.34996065672328	18.530462305604427
13	24.305745886638316	23.763560168689825	32.990663707816495	18.940030236855367
14	20.369871007099473	26.845044957429693	33.27170290079305	19.513381134677783
15	20.224433501021156	26.467968383919647	34.8106218005959	18.496976314463296
16	23.907229437612173	25.218708833228714	32.846110320316164	18.027951408842956
17	24.506219774196115	24.602036125085096	30.588513531434835	20.303230569283954
18	24.780959622304543	25.18743313853254	31.136556534962466	18.895050704200447
19	24.88241222912817	26.772436719212784	29.178565429195363	19.166585622463685
20	25.271203373796492	27.268316726639373	29.3528473038804	18.107632595683736
21	24.367523672274928	26.429840770244105	30.772189166010943	18.430446391470024
22	23.787873429584376	24.064823574137765	31.086824864950884	21.060478131326974
23	22.15755877178247	26.17698285694077	31.292935008443333	20.372523362833423
24	22.35272794787237	25.904342790455054	31.274147488661182	20.468781773011397
25	23.503076732651383	26.265173685094645	29.430870768387457	20.800878813866515
26	21.85198528826686	27.539188555969123	29.537849116323482	21.070977039440532
27	21.802474647899775	26.377567259320823	31.49981875569151	20.320139337087888
28	20.94697940887832	26.977994288593987	31.057648951877425	21.017377350650268
29	21.602000760341976	27.080220499173347	30.467168256606573	20.8506104838781
30	22.783735754639412	25.238601501233344	31.0375352542283	20.940127489898945
31	22.66404820214487	26.28329811594331	30.394007444278426	20.65864623763339
32	23.864791745868956	26.619705234866096	29.083522682062117	20.431980337202827
33	23.569606655644655	26.43823989673495	29.87536138346875	20.116792064151646
34	22.106390409081666	26.590750351437137	31.090582368907317	20.21227687057388
35	21.814852307991547	27.642298885126472	29.584486371312117	20.958362435569857
36	23.246350800569374	27.461828180395553	28.953667765920766	20.338153253114307
37	22.80495460051102	28.5479678534485	28.483206167611204	20.163871378429274
38	22.677420495636873	28.04612004562052	28.199183074433943	21.077276384308664
39	22.7005180934867	26.387624108145385	28.608972035329376	22.30288576303854
40	23.502524158540144	25.68364469042588	29.794906592872234	21.018924558161743
41	21.22116668287551	26.21444738168283	29.369424527217593	23.19496140822407
42	22.211711034683972	26.511511223885346	29.385338661621297	21.891439079809384
43	22.437271786892058	25.799906283430733	29.682291989001563	22.080529940675646
44	22.17391496547517	26.850349668897593	29.003067891465605	21.972667474161632
45	22.16043215716092	26.219531063506235	28.52277047397597	23.097266305356875
46	21.73506060632852	26.311368880794294	28.92780729751474	23.025763215362442
47	21.113414731183745	26.933677844872555	30.112636706835122	21.840270717108577
48	21.854306099534067	26.43183003704457	29.447116447257905	22.266747416163458
49	22.37803584216715	26.059505600891193	29.520277259586052	22.0421812973556
50	20.594105581440584	26.601580804017434	29.969188467557267	22.835125146984716
51	20.919461218138576	26.56444782374212	28.53315886726728	23.982932090852024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	368.0
1	910.0
2	1452.0
3	7579.0
4	13706.0
5	10572.0
6	7438.0
7	5891.0
8	4344.0
9	4223.0
10	4102.0
11	3919.5
12	3737.0
13	3622.5
14	3508.0
15	3493.5
16	3479.0
17	3421.5
18	3364.0
19	3374.0
20	3384.0
21	3596.0
22	3808.0
23	4265.5
24	4723.0
25	6020.5
26	8476.0
27	9634.0
28	11472.5
29	13311.0
30	15537.5
31	17764.0
32	21446.5
33	25129.0
34	27720.5
35	30312.0
36	33078.0
37	35844.0
38	37749.5
39	39655.0
40	42530.5
41	45406.0
42	48842.5
43	52279.0
44	56069.0
45	59859.0
46	64034.0
47	68209.0
48	70227.5
49	72246.0
50	69631.5
51	67017.0
52	62311.0
53	57605.0
54	52933.5
55	48262.0
56	45206.0
57	42150.0
58	40385.0
59	38620.0
60	36492.0
61	34364.0
62	30869.0
63	27374.0
64	23024.5
65	18675.0
66	15640.0
67	12605.0
68	10561.5
69	8518.0
70	7293.5
71	6069.0
72	5011.0
73	3953.0
74	3115.5
75	1810.5
76	1343.0
77	1022.0
78	701.0
79	560.5
80	420.0
81	314.0
82	208.0
83	163.5
84	119.0
85	99.0
86	79.0
87	71.5
88	64.0
89	47.0
90	30.0
91	20.0
92	10.0
93	9.5
94	9.0
95	6.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	904856.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.508928727385246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.30255648060444	27.87009295293509
2	8.490939451271634	6.369720854125827
3	3.89406362000892	4.38186464348455
4	2.3864398068041512	3.5805120250244773
5	1.7562929885357472	3.2938334265696887
6	1.2753792082341633	2.870286469322656
7	0.961376328651806	2.5242137334117123
8	0.7617807516309426	2.285886393505519
9	0.6282147044165381	2.1207294516109787
>10	5.434142913878977	40.421570268045734
>50	0.09446475404045111	2.1949205378211443
>100	0.012854305263706347	0.81595684365357
>500	5.978746634030328E-4	0.188151103973573
>1k	5.978746634030328E-4	0.37428390051477156
>5k	2.989373317015164E-4	0.7079773960006793
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6314	0.6977905876736188	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2063	0.22799207829754128	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1275	0.14090639836614888	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.315444667438797E-4	0.0	0.0	0.04453747336592784	0.0
2	3.315444667438797E-4	0.0	0.0	0.15825722545907858	0.0
3	3.315444667438797E-4	0.0	0.0	0.2540735763480598	0.0
4	3.315444667438797E-4	0.0	0.0	0.36469891341826766	0.0
5	3.315444667438797E-4	0.0	0.0	0.6859655016930871	0.0
6	3.315444667438797E-4	0.0	0.0	1.046906911154924	0.0
7	3.315444667438797E-4	0.0	0.0	1.3651895992290486	0.0
8	3.315444667438797E-4	0.0	0.0	1.9498130089207564	0.0
9	3.315444667438797E-4	0.0	0.0	2.2935141061119118	0.0
10	3.315444667438797E-4	0.0	0.0	2.8491826323746543	0.0
11	3.315444667438797E-4	0.0	0.0	3.4350217051110894	0.0
12	3.315444667438797E-4	0.0	0.0	3.891558435817412	0.0
13	3.315444667438797E-4	0.0	0.0	4.11269859513558	0.0
14	3.315444667438797E-4	0.0	0.0	4.23945909625399	0.0
15	3.315444667438797E-4	0.0	0.0	4.386775354310521	0.0
16	3.315444667438797E-4	0.0	0.0	4.674666466266456	0.0
17	3.315444667438797E-4	0.0	0.0	5.026214115837216	0.0
18	3.315444667438797E-4	0.0	0.0	5.4276039502418065	0.0
19	3.315444667438797E-4	0.0	0.0	5.674494063143749	0.0
20	3.315444667438797E-4	0.0	0.0	5.936745736338158	0.0
21	3.315444667438797E-4	0.0	0.0	6.240440467875551	0.0
22	3.315444667438797E-4	0.0	0.0	6.592982750846543	0.0
23	3.315444667438797E-4	0.0	0.0	6.9398887778828895	0.0
24	3.315444667438797E-4	0.0	0.0	7.223138267304411	0.0
25	3.315444667438797E-4	0.0	0.0	7.480748317964405	0.0
26	3.315444667438797E-4	0.0	0.0	7.729848727311307	0.0
27	3.315444667438797E-4	0.0	0.0	8.004478060597487	0.0
28	3.315444667438797E-4	0.0	0.0	8.267392822725384	0.0
29	3.315444667438797E-4	0.0	0.0	8.57097703944053	0.0
30	3.315444667438797E-4	0.0	0.0	8.934570804636317	0.0
31	3.315444667438797E-4	0.0	0.0	9.249427533220755	0.0
32	3.315444667438797E-4	0.0	0.0	9.57146772525131	0.0
33	3.315444667438797E-4	0.0	0.0	9.879030475567383	0.0
34	3.315444667438797E-4	0.0	0.0	10.207148982821575	0.0
35	3.315444667438797E-4	0.0	0.0	10.5470925760563	0.0
36	3.315444667438797E-4	0.0	0.0	10.878416013155684	0.0
37	3.315444667438797E-4	0.0	0.0	11.231289840593421	0.0
38	3.315444667438797E-4	0.0	0.0	11.603724791569045	0.0
39	3.315444667438797E-4	0.0	0.0	12.19497909059563	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATC	20	7.0324907E-4	45.0	14
CCTACGC	25	3.890146E-5	45.0	34
CAATACG	25	3.890146E-5	45.0	1
CGGTTTA	60	0.0	44.999996	1
CGTTTTT	2655	0.0	41.44068	1
AAGTACG	55	6.002665E-11	40.909092	1
CGTTATT	565	0.0	40.22124	1
CGGTTTT	555	0.0	39.324326	1
CTTACGG	70	0.0	38.571426	2
TACGGGA	485	0.0	38.505157	4
CGTTTTA	335	0.0	37.61194	1
ATTACGG	90	0.0	37.5	2
TTCACGG	60	1.546141E-10	37.499996	2
GTCAACG	30	1.1397301E-4	37.499996	1
TTGTACG	55	2.748493E-9	36.81818	1
CGGGATC	235	0.0	36.38298	6
AGGCGAT	180	0.0	36.25	7
TTACGGG	360	0.0	36.25	3
ACGGGAT	580	0.0	36.077587	5
ACGCACG	25	0.0021068724	36.0	1
>>END_MODULE