FastQCFastQC Report
Sat 18 Jun 2016
SRR3551063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654003
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42970.6570306252417802No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14030.21452500982411396No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8540.13058044076250414No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGG700.045.0000042
CGACGGT207.0310547E-445.028
CACGCGA207.0310547E-445.01
CGTTCAA207.0310547E-445.09
CCGTACA207.0310547E-445.028
GTCAACG207.0310547E-445.01
CGTACAT207.0310547E-445.029
ACCGTAC207.0310547E-445.027
CGCACGA207.0310547E-445.039
TTTGACG207.0310547E-445.01
TATCGAG207.0310547E-445.01
TGCCGTA207.0310547E-445.045
CGTTTTA2350.042.127661
CGTTTTT18550.041.482481
TACGGGT603.6379788E-1241.2499964
CGGTTTT5350.039.953271
GCTACGA800.039.37510
CGTTATT3900.039.2307661
AGGCGAT1150.039.1304327
CGTTGAT356.2441595E-638.5714325