FastQCFastQC Report
Sat 18 Jun 2016
SRR3551060_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551060_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543250
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32880.6052462034054302No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCG21340.3928209848136217No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGC20410.3757017947537966No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTC17810.32784169351127473No Hit
GCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC13970.2571560055223194No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7580.13953060285319835No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGT207.029999E-445.044
CGTTCGG253.8880808E-545.02
AATAGCG207.029999E-445.01
CAATCGA406.8030204E-945.041
TACCGAA207.029999E-445.016
ACGCGTG207.029999E-445.045
CGTTATT1650.042.2727281
CATGCGG700.041.7857132
CGATGAA4100.041.15853519
CGAAACC556.002665E-1140.90909213
CGGTCTA556.002665E-1140.90909231
CGTTTTT14100.040.3723371
GCGAAGT403.4537334E-739.3751
GATCGGT356.2422077E-638.5714269
TAAGTAG356.2422077E-638.5714261
ACTTTCG356.2422077E-638.57142614
TACGGCT3450.038.478267
GCGATTG1000.038.259
ACGGCTG3650.037.602748
CGAAAGG900.037.5000042