##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551060_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	543250
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10850805338242	33.0	31.0	34.0	30.0	34.0
2	32.23158030372757	34.0	31.0	34.0	30.0	34.0
3	32.17564841233318	34.0	31.0	34.0	30.0	34.0
4	35.8094376438104	37.0	35.0	37.0	35.0	37.0
5	31.981496548550393	37.0	35.0	37.0	0.0	37.0
6	33.82782328578003	37.0	35.0	37.0	17.0	37.0
7	35.52312563276576	37.0	35.0	37.0	32.0	37.0
8	35.75019972388403	37.0	35.0	37.0	35.0	37.0
9	37.71684859641049	39.0	37.0	39.0	35.0	39.0
10	37.38532167510354	39.0	37.0	39.0	34.0	39.0
11	37.29536125172572	39.0	37.0	39.0	34.0	39.0
12	37.11691486424298	39.0	37.0	39.0	34.0	39.0
13	36.9849756097561	39.0	37.0	39.0	33.0	39.0
14	38.17750759318914	40.0	37.0	41.0	33.0	41.0
15	38.3276944316613	40.0	37.0	41.0	34.0	41.0
16	38.31371560055223	40.0	37.0	41.0	34.0	41.0
17	38.2651541647492	40.0	37.0	41.0	34.0	41.0
18	38.162713299585825	40.0	37.0	41.0	34.0	41.0
19	38.02870317533364	40.0	37.0	41.0	34.0	41.0
20	38.04118729866544	40.0	36.0	41.0	34.0	41.0
21	37.97709526000921	40.0	36.0	41.0	34.0	41.0
22	38.00486332259549	40.0	36.0	41.0	34.0	41.0
23	38.01158030372756	40.0	36.0	41.0	34.0	41.0
24	37.976237459733085	40.0	35.0	41.0	34.0	41.0
25	37.743803037275654	40.0	35.0	41.0	33.0	41.0
26	37.76231201104464	40.0	35.0	41.0	33.0	41.0
27	37.7399926369075	40.0	35.0	41.0	33.0	41.0
28	37.68437919926369	40.0	35.0	41.0	33.0	41.0
29	37.664458352508056	40.0	35.0	41.0	33.0	41.0
30	37.478243902439026	40.0	35.0	41.0	33.0	41.0
31	37.4766405890474	40.0	35.0	41.0	33.0	41.0
32	37.34256603773585	40.0	35.0	41.0	33.0	41.0
33	37.20534744592729	40.0	35.0	41.0	33.0	41.0
34	37.079317073170735	40.0	35.0	41.0	32.0	41.0
35	37.00252738150023	40.0	35.0	41.0	32.0	41.0
36	36.907142199723886	40.0	35.0	41.0	32.0	41.0
37	36.85435066728026	40.0	35.0	41.0	32.0	41.0
38	36.73931707317073	39.0	35.0	41.0	31.0	41.0
39	36.64326553152324	39.0	35.0	41.0	31.0	41.0
40	36.53927841693511	39.0	35.0	41.0	31.0	41.0
41	36.4611173492867	39.0	35.0	41.0	31.0	41.0
42	36.438709618039574	39.0	35.0	41.0	31.0	41.0
43	36.374365393465254	39.0	35.0	41.0	31.0	41.0
44	36.33107777266452	39.0	35.0	41.0	31.0	41.0
45	36.30200644270594	39.0	35.0	41.0	31.0	41.0
46	36.16491486424298	38.0	35.0	41.0	30.0	41.0
47	36.07410584445467	38.0	35.0	41.0	30.0	41.0
48	36.039598711458815	38.0	35.0	41.0	30.0	41.0
49	36.007374137137596	38.0	35.0	41.0	30.0	41.0
50	35.896662678324894	38.0	35.0	40.0	30.0	41.0
51	34.90928670041417	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	25.0
10	21.0
11	21.0
12	13.0
13	17.0
14	20.0
15	20.0
16	59.0
17	71.0
18	157.0
19	268.0
20	531.0
21	929.0
22	1490.0
23	2081.0
24	2858.0
25	3762.0
26	4813.0
27	5606.0
28	5973.0
29	6688.0
30	8013.0
31	10032.0
32	13354.0
33	20578.0
34	36928.0
35	50048.0
36	41150.0
37	61411.0
38	99383.0
39	166793.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.055867464335016	20.61813161527842	24.05080533824206	12.2751955821445
2	32.49829728485964	25.5462494247584	25.137781868384725	16.81767142199724
3	31.539990796134376	25.316520938794294	27.288173032673726	15.855315232397608
4	28.78619420156466	25.30713299585826	27.810400368154625	18.09627243442246
5	23.88439944776806	36.94505292222733	22.296732627703637	16.873815002300965
6	25.32167510354349	35.81260929590428	24.307225034514495	14.558490566037735
7	78.34569719282098	6.080809940174873	11.420340543028072	4.1531523239760695
8	79.0092959042798	7.428255867464335	9.57017947537966	3.992268752876208
9	73.01150483202944	8.200092038656235	11.352784169351128	7.435618959963184
10	42.9932811780948	26.25421076852278	17.619880349746893	13.132627703635528
11	33.93244362632306	24.660653474459274	23.856787850897376	17.550115048320293
12	31.455499309710078	21.113115508513573	27.96042337781868	19.47096180395766
13	26.480994017487347	24.105476300046018	29.389967786470315	20.02356189599632
14	20.81638288080994	27.517165209387944	29.19226875287621	22.47418315692591
15	20.086516336861482	24.74385641969627	34.52149102623102	20.64813621721123
16	23.997422917625403	24.285503911642888	30.981132075471695	20.73594109526001
17	23.360883571099862	25.11974229176254	28.714588127013347	22.804786010124253
18	24.00147261849977	25.436907501150486	29.187482742751957	21.37413713759779
19	25.636263230556832	27.271974229176255	26.2470317533364	20.84473078693051
20	27.773953060285322	25.744684767602394	27.83856419696272	18.642797975149563
21	25.90501610676484	27.02089277496549	27.987482742751958	19.086608375517716
22	24.29452369995398	24.234330418775883	28.621260929590424	22.849884951679705
23	24.438104003681545	25.59282098481362	29.084215370455592	20.884859641049243
24	24.104739990796133	24.057248044178557	30.085411872986654	21.75260009203866
25	22.406810860561436	27.63258168430741	27.53630924988495	22.424298205246203
26	21.433594109526	26.53345605154165	28.50860561435803	23.52434422457432
27	22.375333640128854	27.034330418775887	28.537137597791073	22.053198343304185
28	19.508329498389323	27.968706856879887	30.176161988034977	22.34680165669581
29	22.24537505752416	25.761619880349745	29.279337321675104	22.71366774045099
30	22.481730326737228	27.050161067648414	28.728209848136217	21.73989875747814
31	24.640589047399907	26.007179015186377	25.68375517717441	23.6684767602393
32	26.200092038656237	27.05292222733548	26.21960423377819	20.527381500230096
33	25.020524620340545	26.266728025770824	26.453198343304184	22.259549010584443
34	22.531799355729408	25.90943396226415	27.981224114127933	23.57754256787851
35	22.42650713299586	27.21325356649793	27.305108145421077	23.055131155085135
36	22.752139898757477	30.402024850437183	25.092130694891857	21.753704555913483
37	23.057892314772204	29.711550851357572	26.158306488725263	21.072250345144962
38	22.1919926369075	28.953888633225954	25.853474459272896	23.000644270593646
39	24.525908881730327	26.64132535664979	26.4108605614358	22.421905200184078
40	23.84887252646111	25.5436723423838	28.517625402669122	22.089829728485963
41	20.65862862402209	27.216935112747354	26.988495167970548	25.135941095260012
42	21.416290842153703	27.12379199263691	26.874367234238377	24.585549930971005
43	22.069581224114128	26.09682466635987	26.960147261849976	24.87344684767602
44	22.297100782328577	26.182420616658998	27.226691210308328	24.293787390704097
45	22.629728485964105	25.833594109526004	26.539162448228254	24.997514956281638
46	22.91320754716981	27.55526921306949	26.68679245283019	22.844730786930512
47	21.038748274275196	25.97809479981592	29.56852277956742	23.414634146341466
48	21.73235158766682	25.070041417395306	29.162448228255865	24.03515876668201
49	21.960239300506213	24.690289921767143	29.544592728946157	23.804878048780488
50	20.486148182236537	25.688725264611133	29.401380579843533	24.42374597330879
51	20.421537045559134	24.155729406350666	28.519466175793834	26.903267372296362
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	319.0
1	754.0
2	1189.0
3	3786.0
4	6383.0
5	4137.0
6	1891.0
7	1784.5
8	1678.0
9	1690.5
10	1703.0
11	1698.5
12	1694.0
13	1619.5
14	1545.0
15	1490.5
16	1436.0
17	1543.5
18	1651.0
19	1650.0
20	1649.0
21	1943.0
22	2237.0
23	2358.0
24	2479.0
25	2987.5
26	4383.5
27	5271.0
28	6099.0
29	6927.0
30	8776.5
31	10626.0
32	11438.0
33	12250.0
34	13711.0
35	15172.0
36	15237.5
37	15303.0
38	16794.5
39	18286.0
40	20355.5
41	22425.0
42	25602.0
43	28779.0
44	32134.5
45	35490.0
46	39344.5
47	43199.0
48	42692.0
49	42185.0
50	42044.5
51	41904.0
52	38539.5
53	35175.0
54	32803.0
55	30431.0
56	29941.5
57	29452.0
58	28413.0
59	27374.0
60	26686.5
61	25999.0
62	23753.0
63	21507.0
64	18654.0
65	15801.0
66	12626.5
67	9452.0
68	8499.0
69	7546.0
70	6084.5
71	4623.0
72	4131.5
73	3640.0
74	3011.5
75	1805.5
76	1228.0
77	986.5
78	745.0
79	573.5
80	402.0
81	290.0
82	178.0
83	107.5
84	37.0
85	50.5
86	64.0
87	51.0
88	38.0
89	20.0
90	2.0
91	3.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	543250.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.29733710657493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.1729727549064	21.437732498907152
2	9.537760853672179	5.588619899438554
3	3.6073206415139616	3.1705466665782196
4	1.9481521518412763	2.2830268130957285
5	1.314228816660541	1.9251702338439456
6	1.002142035291794	1.761605582196768
7	0.7558151640896243	1.550036015681648
8	0.6000805424662932	1.4064609554985072
9	0.5562043509526797	1.4665775733003932
>10	7.100643506902167	48.55025590208313
>50	0.35393571064315316	6.209173615423161
>100	0.046937710730454	2.5325058519434602
>500	6.342933882493784E-4	0.14085995272770346
>1k	0.003171466941246892	1.9774284392816526
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3288	0.6052462034054302	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCG	2134	0.3928209848136217	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGC	2041	0.3757017947537966	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTC	1781	0.32784169351127473	No Hit
GCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	1397	0.2571560055223194	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	758	0.13953060285319835	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8407731247123792E-4	0.0	0.0	0.08946157386102163	0.0
2	1.8407731247123792E-4	0.0	0.0	0.5564657156005522	0.0
3	1.8407731247123792E-4	0.0	0.0	0.728762080073631	0.0
4	1.8407731247123792E-4	0.0	0.0	0.9918085595950299	0.0
5	1.8407731247123792E-4	0.0	0.0	1.954348826507133	0.0
6	1.8407731247123792E-4	0.0	0.0	2.4211688909341924	0.0
7	1.8407731247123792E-4	0.0	0.0	2.920754716981132	0.0
8	1.8407731247123792E-4	0.0	0.0	3.6548550391164287	0.0
9	1.8407731247123792E-4	0.0	0.0	3.904279797514956	0.0
10	1.8407731247123792E-4	0.0	0.0	4.935849056603773	0.0
11	1.8407731247123792E-4	0.0	0.0	5.803221352968246	0.0
12	3.6815462494247585E-4	0.0	0.0	6.873262770363553	0.0
13	5.522319374137137E-4	0.0	0.0	7.170731707317073	0.0
14	5.522319374137137E-4	0.0	0.0	7.293327197422918	0.0
15	5.522319374137137E-4	0.0	0.0	7.566129774505292	0.0
16	5.522319374137137E-4	0.0	0.0	8.017671421997239	0.0
17	5.522319374137137E-4	0.0	0.0	8.548366313851817	0.0
18	5.522319374137137E-4	0.0	0.0	9.098757478140818	0.0
19	5.522319374137137E-4	0.0	0.0	9.531155085135756	0.0
20	5.522319374137137E-4	0.0	0.0	9.898941555453291	0.0
21	5.522319374137137E-4	0.0	0.0	10.361895996318454	0.0
22	5.522319374137137E-4	0.0	0.0	10.84454670961804	0.0
23	5.522319374137137E-4	0.0	0.0	11.299217671421998	0.0
24	7.363092498849517E-4	0.0	0.0	11.684491486424298	0.0
25	7.363092498849517E-4	0.0	0.0	12.003313391624483	0.0
26	7.363092498849517E-4	0.0	0.0	12.327473538886332	0.0
27	7.363092498849517E-4	0.0	0.0	12.641325356649793	0.0
28	7.363092498849517E-4	0.0	0.0	12.953888633225954	0.0
29	7.363092498849517E-4	0.0	0.0	13.293327197422917	0.0
30	9.203865623561896E-4	0.0	0.0	13.683755177174413	0.0
31	9.203865623561896E-4	0.0	0.0	14.0563276576162	0.0
32	9.203865623561896E-4	0.0	0.0	14.39539806718822	0.0
33	9.203865623561896E-4	0.0	0.0	14.723239760699494	0.0
34	9.203865623561896E-4	0.0	0.0	15.050345144960884	0.0
35	9.203865623561896E-4	0.0	0.0	15.43046479521399	0.0
36	9.203865623561896E-4	0.0	0.0	15.767694431661297	0.0
37	0.0011044638748274275	0.0	0.0	16.136033133916246	0.0
38	0.0011044638748274275	0.0	0.0	16.509526000920385	0.0
39	0.0014726184997699034	0.0	0.0	16.86700414173953	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCGT	20	7.029999E-4	45.0	44
CGTTCGG	25	3.8880808E-5	45.0	2
AATAGCG	20	7.029999E-4	45.0	1
CAATCGA	40	6.8030204E-9	45.0	41
TACCGAA	20	7.029999E-4	45.0	16
ACGCGTG	20	7.029999E-4	45.0	45
CGTTATT	165	0.0	42.272728	1
CATGCGG	70	0.0	41.785713	2
CGATGAA	410	0.0	41.158535	19
CGAAACC	55	6.002665E-11	40.909092	13
CGGTCTA	55	6.002665E-11	40.909092	31
CGTTTTT	1410	0.0	40.372337	1
GCGAAGT	40	3.4537334E-7	39.375	1
GATCGGT	35	6.2422077E-6	38.571426	9
TAAGTAG	35	6.2422077E-6	38.571426	1
ACTTTCG	35	6.2422077E-6	38.571426	14
TACGGCT	345	0.0	38.47826	7
GCGATTG	100	0.0	38.25	9
ACGGCTG	365	0.0	37.60274	8
CGAAAGG	90	0.0	37.500004	2
>>END_MODULE