FastQCFastQC Report
Sat 18 Jun 2016
SRR3551059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894535
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71970.8045520857205141No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC17240.19272582962097629No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC14780.1652255082249437No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG11640.13012347197147123No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC9160.10239957072669041No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC9000.10061093193670455No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG207.032447E-445.01
AACGGCG207.032447E-445.02
ACGTCAC207.032447E-445.030
CAATCGT207.032447E-445.040
TTCGACA253.8901093E-545.08
CGATAAT253.8901093E-545.012
CGTTTTT43150.043.018541
CGTTATT6500.040.1538471
GCGTTAG451.9274012E-840.01
TAGCGCG403.4570803E-739.3751
GCGCTAG403.4570803E-739.3751
AGTCGAG403.4570803E-739.3751
CGTTCGG356.246737E-638.5714262
GTCGTAA356.246737E-638.57142626
CGTCGTA356.246737E-638.57142625
GTATGCG659.094947E-1238.076921
GCGAGAC1250.037.821
CGACGGT301.1397193E-437.50000428
ACGGACG301.1397193E-437.5000041
GCGATCG301.1397193E-437.5000049