##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551059_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	894535
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11026063820868	33.0	31.0	34.0	30.0	34.0
2	32.235962818671155	34.0	31.0	34.0	30.0	34.0
3	32.14565668196325	33.0	31.0	34.0	30.0	34.0
4	35.7855858071512	37.0	35.0	37.0	35.0	37.0
5	31.981391449188685	37.0	35.0	37.0	0.0	37.0
6	33.836029892625774	37.0	35.0	37.0	17.0	37.0
7	35.55518789091539	37.0	35.0	37.0	32.0	37.0
8	35.82925095161173	37.0	35.0	37.0	35.0	37.0
9	37.815261560475555	39.0	38.0	39.0	35.0	39.0
10	37.349769433280976	39.0	37.0	39.0	34.0	39.0
11	37.32896309255647	39.0	37.0	39.0	34.0	39.0
12	37.36809068398665	39.0	37.0	39.0	35.0	39.0
13	37.37233646531438	39.0	37.0	39.0	35.0	39.0
14	38.65206392147876	40.0	38.0	41.0	35.0	41.0
15	38.77391493904654	40.0	38.0	41.0	35.0	41.0
16	38.69073652791674	40.0	38.0	41.0	35.0	41.0
17	38.68444275517447	40.0	38.0	41.0	35.0	41.0
18	38.554483614391835	40.0	38.0	41.0	35.0	41.0
19	38.370563477113805	40.0	37.0	41.0	34.0	41.0
20	38.40203010502663	40.0	37.0	41.0	34.0	41.0
21	38.326709407681086	40.0	37.0	41.0	34.0	41.0
22	38.38585745666743	40.0	37.0	41.0	34.0	41.0
23	38.394357962516835	40.0	37.0	41.0	34.0	41.0
24	38.35909718457076	40.0	37.0	41.0	34.0	41.0
25	38.10626303051306	40.0	37.0	41.0	34.0	41.0
26	38.167469132007135	40.0	37.0	41.0	34.0	41.0
27	38.15975898092305	40.0	37.0	41.0	34.0	41.0
28	38.10406971219684	40.0	37.0	41.0	34.0	41.0
29	38.08667631786347	40.0	37.0	41.0	34.0	41.0
30	37.91622574857328	40.0	36.0	41.0	34.0	41.0
31	37.87307595566411	40.0	37.0	41.0	34.0	41.0
32	37.72193262421258	40.0	37.0	41.0	33.0	41.0
33	37.564254053782136	40.0	37.0	41.0	33.0	41.0
34	37.39330601932848	40.0	36.0	41.0	33.0	41.0
35	37.29823986764073	40.0	36.0	41.0	33.0	41.0
36	37.18149988541533	40.0	36.0	41.0	32.0	41.0
37	37.13423734118844	40.0	36.0	41.0	32.0	41.0
38	37.0268273460513	40.0	36.0	41.0	31.0	41.0
39	36.96054821778913	40.0	36.0	41.0	31.0	41.0
40	36.8985763553131	40.0	36.0	41.0	31.0	41.0
41	36.78713297970454	40.0	35.0	41.0	31.0	41.0
42	36.76349388229639	40.0	35.0	41.0	31.0	41.0
43	36.70877830381148	40.0	35.0	41.0	31.0	41.0
44	36.65282968246072	40.0	35.0	41.0	31.0	41.0
45	36.634038914072676	40.0	35.0	41.0	31.0	41.0
46	36.51716478393802	40.0	35.0	41.0	30.0	41.0
47	36.451728551705635	39.0	35.0	41.0	30.0	41.0
48	36.407514518716425	39.0	35.0	41.0	30.0	41.0
49	36.36696943104518	39.0	35.0	41.0	30.0	41.0
50	36.2686848474347	39.0	35.0	41.0	30.0	41.0
51	35.32342390180373	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	17.0
9	37.0
10	40.0
11	23.0
12	21.0
13	15.0
14	25.0
15	20.0
16	74.0
17	112.0
18	241.0
19	409.0
20	730.0
21	1243.0
22	1851.0
23	2957.0
24	4596.0
25	7121.0
26	9744.0
27	10732.0
28	10631.0
29	10715.0
30	11857.0
31	14408.0
32	18324.0
33	26542.0
34	43555.0
35	60480.0
36	62226.0
37	99461.0
38	173145.0
39	322936.0
40	242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.290614676899175	20.2276042860257	24.306147886890955	14.175633150184176
2	34.85799884856378	23.660337493781686	24.76068571939611	16.720977938258425
3	31.118737668173967	23.572470613223633	29.38275193256832	15.926039786034085
4	26.963394389263694	24.911602117301168	29.60029512540035	18.52470836803479
5	22.28968123103065	36.715723811812836	24.892038880535697	16.102556076620814
6	24.314084971521517	33.696836904089835	27.84586405227297	14.143214072115681
7	79.22462508454113	5.621468137076805	11.241259425287998	3.9126473530940653
8	80.97760288865165	5.097844131308445	10.455935206559833	3.468617773480076
9	75.11992264137234	7.190327935743151	12.380734124433365	5.3090152984511505
10	35.1744761244669	31.261381611675336	20.33648767236609	13.22765459149167
11	26.12374026728971	25.825820118832688	30.180261253053263	17.870178360824337
12	23.718691834304977	23.629818844427554	33.197918471608155	19.45357084965932
13	22.636900736136653	24.10805614090002	34.52464129408017	18.73040182888316
14	20.18199399688106	27.654032542046984	32.68446734895784	19.479506112114116
15	19.439932478885677	26.71387927806067	35.05072467818475	18.795463564868896
16	22.262292699558987	26.646469953663075	32.56630539889439	18.524931947883537
17	22.385820565992386	26.17192172469495	31.431861246345864	20.010396462966792
18	22.679380907398816	25.60067521114322	32.503814831169265	19.216129050288696
19	22.57195079007529	27.630333078079673	30.447550962231773	19.350165169613263
20	23.707065682170068	27.200053659163697	31.402013336537976	17.690867322128256
21	22.871435997473547	27.609763731994835	31.620003689067506	17.89879658146411
22	22.00808241153225	25.016461066364087	31.847496185168833	21.12796033693483
23	20.289647694053333	27.36024862079181	31.971918370997223	20.378185314157633
24	20.41664104814233	26.341730619819238	32.88501847328501	20.35660985875343
25	20.438551873319657	28.22427294627935	30.911926308081856	20.425248872319138
26	20.923049405557077	27.818140151028185	30.794546887489034	20.464263555925704
27	20.805669984964258	27.494061160267623	31.540521052837505	20.15974780193061
28	19.148719725891105	27.291944976999222	32.77736477611273	20.781970520996943
29	20.738596030339785	25.792842091142326	32.01506928180563	21.45349259671226
30	20.54531125109694	26.093445197784327	31.528335951080727	21.83290760003801
31	21.538676519085335	26.410369633384946	30.466443459451	21.584510388078723
32	21.528056476269793	27.10469685367258	30.24286361070277	21.12438305935486
33	20.72786419759987	26.525736835339032	29.73131291676681	23.015086050294286
34	19.708899036929804	26.157724404299444	31.862476035034966	22.270900523735797
35	20.087866880558057	26.936676597338284	30.694383115249824	22.281073406853842
36	20.522170736751498	27.653585382349487	30.36516178796805	21.459082092930963
37	19.72868585354402	27.900864695065035	31.089560497912323	21.280888953478623
38	19.435013722213217	28.20102064200953	29.682013560117827	22.68195207565942
39	20.316812645676247	26.343183888836098	30.77822555853041	22.561777906957246
40	20.885487990967373	26.462799107916403	31.045068108011424	21.6066447931048
41	19.81331082629523	27.309272415277213	30.52367990073055	22.353736857697015
42	20.656654015773558	26.751328902725994	30.77040026382422	21.821616817676222
43	21.05976848306662	26.127652914642802	30.4638722911904	22.34870631110018
44	20.383663020451966	26.690962343563974	29.7766996260627	23.148675009921355
45	21.154678128860247	26.006584426545636	29.48146243579066	23.35727500880346
46	21.38373568390281	26.488063630824954	29.55714421459194	22.571056470680297
47	19.953942551157862	26.818402857350463	31.144113981006893	22.08354061048478
48	19.96981672041899	25.887416367162828	31.320518481669247	22.822248430748935
49	20.75044576232344	25.425612189573354	31.490998116339775	22.33294393176343
50	19.506559273812652	25.779427300217435	31.078046135701786	23.635967290268127
51	19.641601502456584	25.56859150284785	29.510304236279183	25.279502758416385
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	328.0
1	721.0
2	1114.0
3	7192.5
4	13271.0
5	8860.5
6	4450.0
7	4297.0
8	4144.0
9	4152.0
10	4160.0
11	4148.0
12	4136.0
13	4069.0
14	4002.0
15	3870.0
16	3738.0
17	3759.0
18	3780.0
19	3912.0
20	4044.0
21	4297.5
22	4551.0
23	4683.0
24	4815.0
25	6250.5
26	9083.0
27	10480.0
28	12431.0
29	14382.0
30	16874.0
31	19366.0
32	22184.0
33	25002.0
34	27619.5
35	30237.0
36	33692.5
37	37148.0
38	38792.5
39	40437.0
40	44178.5
41	47920.0
42	51352.0
43	54784.0
44	59322.0
45	63860.0
46	71400.5
47	78941.0
48	79907.5
49	80874.0
50	79022.0
51	77170.0
52	68429.0
53	59688.0
54	53811.0
55	47934.0
56	42356.5
57	36779.0
58	33758.5
59	30738.0
60	27522.5
61	24307.0
62	21571.0
63	18835.0
64	15713.5
65	12592.0
66	10094.5
67	7597.0
68	6164.5
69	4732.0
70	3590.5
71	2449.0
72	2057.0
73	1665.0
74	1386.5
75	846.5
76	585.0
77	493.5
78	402.0
79	301.5
80	201.0
81	133.5
82	66.0
83	42.5
84	19.0
85	14.5
86	10.0
87	7.0
88	4.0
89	2.5
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	894535.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.59841275508825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.1480952005823	23.845117995970718
2	9.445383301753434	6.158090070011533
3	3.936383634221589	3.8495957541218906
4	2.172580922786984	2.8329075865936244
5	1.4662941095103588	2.3899430301086624
6	1.0789784495043582	2.1103790910472933
7	0.8337919432831692	1.9026205743308315
8	0.6565031930157599	1.7120769648768888
9	0.5795427543163976	1.7002956522983965
>10	6.428013288359716	45.74284071293295
>50	0.22488837557265018	4.428628733295412
>100	0.026068965082347316	1.4916943501220319
>500	0.0020855172065874054	0.5256338845587055
>1k	0.0010427586032937027	0.49470091398648597
>5k	3.475862010979009E-4	0.8154746857445577
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7197	0.8045520857205141	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC	1724	0.19272582962097629	No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	1478	0.1652255082249437	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG	1164	0.13012347197147123	No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	916	0.10239957072669041	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC	900	0.10061093193670455	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.825294706188131E-4	0.0	0.0	0.09580396518861754	0.0
2	7.825294706188131E-4	0.0	0.0	0.4746600188924972	0.0
3	7.825294706188131E-4	0.0	0.0	0.6797945301190004	0.0
4	7.825294706188131E-4	0.0	0.0	0.9678771652310977	0.0
5	7.825294706188131E-4	0.0	0.0	1.7933339668095714	0.0
6	7.825294706188131E-4	0.0	0.0	2.453118100465605	0.0
7	7.825294706188131E-4	0.0	0.0	3.0346492870597572	0.0
8	7.825294706188131E-4	0.0	0.0	3.84568518839397	0.0
9	7.825294706188131E-4	0.0	0.0	4.20687843404674	0.0
10	7.825294706188131E-4	0.0	0.0	5.041278429575143	0.0
11	7.825294706188131E-4	0.0	0.0	5.973047449233401	0.0
12	7.825294706188131E-4	0.0	0.0	6.795709502702522	0.0
13	7.825294706188131E-4	0.0	0.0	7.1273902083205245	0.0
14	7.825294706188131E-4	0.0	0.0	7.277859446528084	0.0
15	7.825294706188131E-4	0.0	0.0	7.500656765805698	0.0
16	7.825294706188131E-4	0.0	0.0	7.938985059276607	0.0
17	7.825294706188131E-4	0.0	0.0	8.47870681415484	0.0
18	7.825294706188131E-4	0.0	0.0	9.03978044458853	0.0
19	7.825294706188131E-4	0.0	0.0	9.446695769310312	0.0
20	8.943193949929293E-4	0.0	0.0	9.800622669878765	0.0
21	8.943193949929293E-4	0.0	0.0	10.251695014728323	0.0
22	8.943193949929293E-4	0.0	0.0	10.757768002369946	0.0
23	8.943193949929293E-4	0.0	0.0	11.269206906381529	0.0
24	0.0010061093193670454	0.0	0.0	11.677016550498303	0.0
25	0.0010061093193670454	0.0	0.0	12.050953847529723	0.0
26	0.0010061093193670454	0.0	0.0	12.400520941047583	0.0
27	0.0010061093193670454	0.0	0.0	12.766856523221563	0.0
28	0.0010061093193670454	0.0	0.0	13.13945234116049	0.0
29	0.0010061093193670454	0.0	0.0	13.536082992839855	0.0
30	0.0010061093193670454	0.0	0.0	14.005936044984265	0.0
31	0.0010061093193670454	0.0	0.0	14.403013856361126	0.0
32	0.0010061093193670454	0.0	0.0	14.810264550856031	0.0
33	0.0010061093193670454	0.0	0.0	15.198622748131712	0.0
34	0.0010061093193670454	0.0	0.0	15.588881374121751	0.0
35	0.0010061093193670454	0.0	0.0	16.00116261521349	0.0
36	0.0010061093193670454	0.0	0.0	16.39063871173291	0.0
37	0.0010061093193670454	0.0	0.0	16.78570430447104	0.0
38	0.0010061093193670454	0.0	0.0	17.188930561688476	0.0
39	0.0010061093193670454	0.0	0.0	17.613173324688248	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACG	20	7.032447E-4	45.0	1
AACGGCG	20	7.032447E-4	45.0	2
ACGTCAC	20	7.032447E-4	45.0	30
CAATCGT	20	7.032447E-4	45.0	40
TTCGACA	25	3.8901093E-5	45.0	8
CGATAAT	25	3.8901093E-5	45.0	12
CGTTTTT	4315	0.0	43.01854	1
CGTTATT	650	0.0	40.153847	1
GCGTTAG	45	1.9274012E-8	40.0	1
TAGCGCG	40	3.4570803E-7	39.375	1
GCGCTAG	40	3.4570803E-7	39.375	1
AGTCGAG	40	3.4570803E-7	39.375	1
CGTTCGG	35	6.246737E-6	38.571426	2
GTCGTAA	35	6.246737E-6	38.571426	26
CGTCGTA	35	6.246737E-6	38.571426	25
GTATGCG	65	9.094947E-12	38.07692	1
GCGAGAC	125	0.0	37.8	21
CGACGGT	30	1.1397193E-4	37.500004	28
ACGGACG	30	1.1397193E-4	37.500004	1
GCGATCG	30	1.1397193E-4	37.500004	9
>>END_MODULE