FastQCFastQC Report
Sat 18 Jun 2016
SRR3551057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521730
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31300.599927165392061No Hit
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC9360.17940313955494222TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC9290.178061449408698No Hit
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC9020.17288635884461312TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG8880.17020297855212466No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8520.1633028578000115No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC7170.13742740497958714No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT5600.10733521169953807TruSeq Adapter, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC453.8380676E-1045.09
CGTTATT1400.045.01
TCGTAAT207.029742E-445.022
ACCGTTG207.029742E-445.01
CGGTCTA253.8878672E-545.031
CGCCGGT453.8380676E-1045.028
TATACGG207.029742E-445.02
TACGTAG253.8878672E-545.01
TAGTACG207.029742E-445.01
CGAAGGC207.029742E-445.022
CTAGCGC253.8878672E-545.024
TAAACGG302.1632131E-644.9999962
GCTACGA1150.043.04347610
TACGGGA2050.041.7073174
GTTAGCG603.6379788E-1241.2499961
CGGTAGT603.6379788E-1241.24999612
TGCGATG556.002665E-1140.9090921
TGACGGG1900.040.2631573
AAGGGCG1850.040.1351365
ACTACGG451.9248546E-840.02