##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551057_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	521730
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21992218197152	33.0	31.0	34.0	30.0	34.0
2	32.33753090679087	34.0	31.0	34.0	30.0	34.0
3	32.2728844421444	34.0	31.0	34.0	30.0	34.0
4	35.87828186993272	37.0	35.0	37.0	35.0	37.0
5	32.12177754777375	37.0	35.0	37.0	0.0	37.0
6	33.931698388055125	37.0	35.0	37.0	17.0	37.0
7	35.59382822532728	37.0	35.0	37.0	32.0	37.0
8	35.82785540413624	37.0	35.0	37.0	35.0	37.0
9	37.774367968106105	39.0	38.0	39.0	35.0	39.0
10	37.47510973108696	39.0	37.0	39.0	35.0	39.0
11	37.42173538036916	39.0	37.0	39.0	35.0	39.0
12	37.349730703620644	39.0	37.0	39.0	35.0	39.0
13	37.267278094033315	39.0	37.0	39.0	34.0	39.0
14	38.54534529354264	40.0	38.0	41.0	34.0	41.0
15	38.68605983938052	40.0	38.0	41.0	35.0	41.0
16	38.627433730090274	40.0	38.0	41.0	35.0	41.0
17	38.602599045483295	40.0	38.0	41.0	35.0	41.0
18	38.50062101086769	40.0	38.0	41.0	35.0	41.0
19	38.363138021582046	40.0	37.0	41.0	34.0	41.0
20	38.40051942575662	40.0	37.0	41.0	34.0	41.0
21	38.32998102466793	40.0	37.0	41.0	34.0	41.0
22	38.38426772468518	40.0	37.0	41.0	34.0	41.0
23	38.37676767676768	40.0	37.0	41.0	34.0	41.0
24	38.340839131351466	40.0	37.0	41.0	34.0	41.0
25	38.08404538746095	40.0	36.0	41.0	34.0	41.0
26	38.12994077396355	40.0	36.0	41.0	34.0	41.0
27	38.11470492400284	40.0	36.0	41.0	34.0	41.0
28	38.03304774500221	40.0	36.0	41.0	34.0	41.0
29	38.01406857953347	40.0	36.0	41.0	34.0	41.0
30	37.83324133172331	40.0	36.0	41.0	34.0	41.0
31	37.81412799723995	40.0	36.0	41.0	34.0	41.0
32	37.710043509094746	40.0	36.0	41.0	33.0	41.0
33	37.59858547524582	40.0	36.0	41.0	33.0	41.0
34	37.49334521687463	40.0	36.0	41.0	33.0	41.0
35	37.421219788012955	40.0	36.0	41.0	33.0	41.0
36	37.315132348149426	40.0	35.0	41.0	33.0	41.0
37	37.25903244973453	40.0	35.0	41.0	33.0	41.0
38	37.15185632415234	40.0	35.0	41.0	32.0	41.0
39	37.080811914208496	40.0	35.0	41.0	32.0	41.0
40	36.98821996051598	40.0	35.0	41.0	32.0	41.0
41	36.88458973032028	40.0	35.0	41.0	31.0	41.0
42	36.85735533705173	40.0	35.0	41.0	32.0	41.0
43	36.80922316140533	40.0	35.0	41.0	32.0	41.0
44	36.74588963640197	39.0	35.0	41.0	31.0	41.0
45	36.72620512525636	39.0	35.0	41.0	31.0	41.0
46	36.59389339313438	39.0	35.0	41.0	31.0	41.0
47	36.526944971537	39.0	35.0	41.0	31.0	41.0
48	36.48011423533245	39.0	35.0	41.0	31.0	41.0
49	36.42806815785943	39.0	35.0	41.0	31.0	41.0
50	36.34019895348169	39.0	35.0	41.0	31.0	41.0
51	35.346968738619594	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	29.0
10	20.0
11	15.0
12	14.0
13	5.0
14	11.0
15	19.0
16	36.0
17	71.0
18	125.0
19	232.0
20	438.0
21	750.0
22	1044.0
23	1631.0
24	2329.0
25	3287.0
26	4427.0
27	5047.0
28	5388.0
29	5802.0
30	6671.0
31	8229.0
32	10787.0
33	16489.0
34	29578.0
35	41362.0
36	36570.0
37	57737.0
38	98685.0
39	184740.0
40	153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.68587200276005	20.64516129032258	23.972169512966477	12.696797193950893
2	33.136871561919	24.657581507676383	25.233933260498727	16.971613669905892
3	32.0422440726046	23.808866655166465	27.80729496099515	16.341594311233777
4	28.757594924577845	25.335710041592396	27.095624173423033	18.811070860406723
5	23.16044697448872	36.84568646617982	23.263565445728634	16.73030111360282
6	25.164165372894026	35.093439135184866	25.37787744618864	14.364518045732467
7	78.56170816322619	6.264542962835183	10.677361853832442	4.4963870201061855
8	79.63716865045139	6.907020872865275	9.376114081996436	4.0796963946869065
9	72.49899373239032	8.728077741360474	12.006401778697793	6.766526747551415
10	39.67876104498495	27.784102888467217	18.840588043624095	13.696548022923736
11	31.498859563375692	25.77444271941426	25.195407586299428	17.531290130910623
12	29.829605351426984	22.41638395338585	28.435014279416553	19.31899641577061
13	25.016962796848947	24.109980257987846	30.80558909780921	20.067467847353996
14	20.176336419220668	28.022348724436014	30.070151227646484	21.731163628696837
15	19.695436336802562	26.65765817568474	34.23284074137964	19.414064746133057
16	22.355241216721293	24.295516838211334	32.665363310524604	20.683878634542772
17	22.21129703103138	25.08998907480881	29.41808981657179	23.280624077588026
18	22.351024476261667	25.8624192590037	30.08740152952677	21.699154735207866
19	24.60161386157591	27.313936327219057	27.843712264964637	20.240737546240393
20	26.984072221263872	25.311942959001783	28.756636574473387	18.947348245260958
21	25.171832173729708	26.855844977287102	28.413163897034867	19.559158951948323
22	23.27525731700305	25.462212255381132	28.54867460180553	22.713855825810285
23	23.13897993214881	26.728192743373008	29.18616909129243	20.946658233185747
24	22.979893814808424	25.207482797615626	30.265654648956357	21.546968738619594
25	21.994326567381595	26.87712034960612	28.377513273148946	22.75103980986334
26	21.408582983535545	27.757460755563223	29.13499319571426	21.698963065186973
27	22.78879880397907	27.556015563605698	29.039158185268242	20.616027447146994
28	20.090851589902822	27.65491729438598	31.427749985624747	20.826481130086442
29	21.893508136392388	26.47557165583731	30.835297951047476	20.795622256722826
30	22.696030513867328	26.971613669905892	29.461790581335173	20.87056523489161
31	23.56122898817396	27.375079063883618	27.972897859045865	21.090794088896555
32	24.968661951584153	27.461522243305925	27.432196730109442	20.13761907500048
33	23.505453012094378	27.786594598738812	27.436988480631747	21.270963908535066
34	23.149521783297875	27.093707473214113	28.70986908937573	21.04690165411228
35	23.385467579015966	26.02380541659479	28.268644701282277	22.32208230310697
36	23.017652808924154	28.922431142544998	27.68845954804209	20.371456500488758
37	23.821325206524445	27.84390393498553	28.578191785022906	19.75657907346712
38	22.25768117608725	28.959231786556266	26.79546892070611	21.987618116650374
39	23.46807735802043	27.229409848005677	27.42913000977517	21.87338278419872
40	23.56851244896786	26.661108236060798	29.117359553792195	20.653019761179152
41	20.98365054721791	26.674908477565022	29.301746113890324	23.03969486132674
42	21.77198934314684	27.324669848389014	28.423130738121248	22.4802100703429
43	22.724397676959345	26.657849845705634	28.116458704693997	22.50129377264102
44	22.234489103559312	27.323903168305446	27.80499492074445	22.636612807390797
45	23.11712954976712	26.331244130105606	27.212542886167174	23.339083433960095
46	22.04090238245836	28.389013474402468	27.641692062944433	21.928392080194737
47	22.181779847814003	26.899162402008702	29.627585149406784	21.291472600770515
48	21.855557472255764	25.933720506775536	29.6126348877772	22.5980871331915
49	21.671937592241196	25.63548195426753	29.744312192130028	22.94826826136124
50	20.546259559542293	26.41979567975773	29.305579514308167	23.72836524639181
51	20.156786077089684	26.26358461273072	28.2945201541027	25.285109156076896
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	272.0
1	732.5
2	1193.0
3	3601.5
4	6010.0
5	3969.5
6	1929.0
7	1913.5
8	1898.0
9	1843.0
10	1788.0
11	1810.5
12	1833.0
13	1868.5
14	1904.0
15	1822.0
16	1740.0
17	1675.0
18	1610.0
19	1814.0
20	2018.0
21	1931.5
22	1845.0
23	2246.5
24	2648.0
25	3276.0
26	4676.0
27	5448.0
28	6244.0
29	7040.0
30	8504.5
31	9969.0
32	11507.0
33	13045.0
34	14319.5
35	15594.0
36	16710.0
37	17826.0
38	19505.0
39	21184.0
40	23133.5
41	25083.0
42	27617.0
43	30151.0
44	32823.5
45	35496.0
46	38731.5
47	41967.0
48	42584.0
49	43201.0
50	41964.5
51	40728.0
52	36569.0
53	32410.0
54	29923.5
55	27437.0
56	26764.5
57	26092.0
58	25429.0
59	24766.0
60	23743.0
61	22720.0
62	20306.0
63	17892.0
64	15270.0
65	12648.0
66	10225.5
67	7803.0
68	6250.0
69	4697.0
70	4150.0
71	3603.0
72	2847.0
73	2091.0
74	1610.5
75	809.5
76	489.0
77	411.0
78	333.0
79	249.5
80	166.0
81	130.0
82	94.0
83	56.0
84	18.0
85	13.0
86	8.0
87	6.0
88	4.0
89	4.0
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	521730.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.166642410523515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.74436130671049	21.21708773614748
2	9.230974695090158	5.384730760645719
3	3.5877770433842304	3.139302302192195
4	2.082597423119404	2.4296949730080555
5	1.4856116420857932	2.1665151762813486
6	1.0435736945625194	1.826252446700033
7	0.8005786773020523	1.63451344016512
8	0.7095204386092763	1.6555463132699648
9	0.5468879267100273	1.4355796137285561
>10	7.3995119317104185	49.67432539688466
>50	0.3221144721568603	5.722231542933394
>100	0.04117752015201101	1.9874778480195203
>500	0.00464907485587221	1.1204261084741622
>1k	6.641535508388872E-4	0.6063163415498146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3130	0.599927165392061	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	936	0.17940313955494222	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	929	0.178061449408698	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	902	0.17288635884461312	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	888	0.17020297855212466	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	852	0.1633028578000115	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	717	0.13742740497958714	No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	560	0.10733521169953807	TruSeq Adapter, Index 14 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8334004178406457E-4	0.0	0.0	0.11078527207559466	0.0
2	3.8334004178406457E-4	0.0	0.0	0.5790351331148295	0.0
3	3.8334004178406457E-4	0.0	0.0	0.7624633431085044	0.0
4	3.8334004178406457E-4	0.0	0.0	0.9869089375730742	0.0
5	3.8334004178406457E-4	0.0	0.0	1.680179403139555	0.0
6	3.8334004178406457E-4	0.0	0.0	2.119487091024093	0.0
7	3.8334004178406457E-4	0.0	0.0	2.5758534107680218	0.0
8	3.8334004178406457E-4	0.0	0.0	3.273340616794127	0.0
9	3.8334004178406457E-4	0.0	0.0	3.532286815019263	0.0
10	3.8334004178406457E-4	0.0	0.0	4.260632894408985	0.0
11	3.8334004178406457E-4	0.0	0.0	5.062771931842141	0.0
12	3.8334004178406457E-4	0.0	0.0	5.834818775995247	0.0
13	3.8334004178406457E-4	0.0	0.0	6.094148314262166	0.0
14	3.8334004178406457E-4	0.0	0.0	6.203783566212409	0.0
15	3.8334004178406457E-4	0.0	0.0	6.430912540969467	0.0
16	3.8334004178406457E-4	0.0	0.0	6.831886224675599	0.0
17	3.8334004178406457E-4	0.0	0.0	7.279819063500278	0.0
18	3.8334004178406457E-4	0.0	0.0	7.797711459950549	0.0
19	3.8334004178406457E-4	0.0	0.0	8.15440936883062	0.0
20	3.8334004178406457E-4	0.0	0.0	8.489640235370786	0.0
21	3.8334004178406457E-4	0.0	0.0	8.864163456193817	0.0
22	3.8334004178406457E-4	0.0	0.0	9.29829605351427	0.0
23	3.8334004178406457E-4	0.0	0.0	9.70099476740843	0.0
24	3.8334004178406457E-4	0.0	0.0	10.03584229390681	0.0
25	3.8334004178406457E-4	0.0	0.0	10.332547486247677	0.0
26	3.8334004178406457E-4	0.0	0.0	10.610660686562015	0.0
27	3.8334004178406457E-4	0.0	0.0	10.890882257106165	0.0
28	3.8334004178406457E-4	0.0	0.0	11.17972897859046	0.0
29	3.8334004178406457E-4	0.0	0.0	11.491001092519118	0.0
30	3.8334004178406457E-4	0.0	0.0	11.842907250876891	0.0
31	3.8334004178406457E-4	0.0	0.0	12.173346366894753	0.0
32	3.8334004178406457E-4	0.0	0.0	12.482510110593601	0.0
33	3.8334004178406457E-4	0.0	0.0	12.798957315086348	0.0
34	3.8334004178406457E-4	0.0	0.0	13.116554539704445	0.0
35	3.8334004178406457E-4	0.0	0.0	13.44526862553428	0.0
36	3.8334004178406457E-4	0.0	0.0	13.767082590612002	0.0
37	3.8334004178406457E-4	0.0	0.0	14.099246736817895	0.0
38	3.8334004178406457E-4	0.0	0.0	14.436202633546086	0.0
39	5.750100626760969E-4	0.0	0.0	14.782167021256205	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	45	3.8380676E-10	45.0	9
CGTTATT	140	0.0	45.0	1
TCGTAAT	20	7.029742E-4	45.0	22
ACCGTTG	20	7.029742E-4	45.0	1
CGGTCTA	25	3.8878672E-5	45.0	31
CGCCGGT	45	3.8380676E-10	45.0	28
TATACGG	20	7.029742E-4	45.0	2
TACGTAG	25	3.8878672E-5	45.0	1
TAGTACG	20	7.029742E-4	45.0	1
CGAAGGC	20	7.029742E-4	45.0	22
CTAGCGC	25	3.8878672E-5	45.0	24
TAAACGG	30	2.1632131E-6	44.999996	2
GCTACGA	115	0.0	43.043476	10
TACGGGA	205	0.0	41.707317	4
GTTAGCG	60	3.6379788E-12	41.249996	1
CGGTAGT	60	3.6379788E-12	41.249996	12
TGCGATG	55	6.002665E-11	40.909092	1
TGACGGG	190	0.0	40.263157	3
AAGGGCG	185	0.0	40.135136	5
ACTACGG	45	1.9248546E-8	40.0	2
>>END_MODULE