FastQCFastQC Report
Sat 18 Jun 2016
SRR3551051_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551051_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730303
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84451.1563693425879396No Hit
GCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC17880.24482988567758862No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10420.14268050384566405No Hit
CTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGCT10050.13761411359394662No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCC9410.12885062775313808No Hit
CCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC7520.10297095862950036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATT351.2111195E-745.00000437
CATACGG302.16441E-645.0000042
AACCGGC207.031596E-445.036
TCCGCGC207.031596E-445.013
GATCGGT207.031596E-445.023
CGTTCTA207.031596E-445.031
CGTAGCG253.889404E-545.02
TTACGAA207.031596E-445.031
CCAACGG207.031596E-445.02
TTCGACG207.031596E-445.025
AAGTCGA207.031596E-445.044
CGTTATT4500.043.51
CGTTTTT33850.043.072381
CGTAAGG850.042.352942
CGTATGG800.042.18752
ACAACGG603.6379788E-1241.2500042
TAAGGGA6300.041.071434
CCATCGA501.0804797E-940.531
ATACGGA403.4559162E-739.37534
ATACGGG2200.038.8636363