##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551051_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	730303
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.036931246345695	33.0	31.0	34.0	30.0	34.0
2	32.148141250960215	33.0	31.0	34.0	30.0	34.0
3	32.06288622667578	33.0	31.0	34.0	30.0	34.0
4	35.72388583916539	37.0	35.0	37.0	33.0	37.0
5	31.86244202748722	37.0	35.0	37.0	0.0	37.0
6	33.76181940920412	37.0	35.0	37.0	17.0	37.0
7	35.462121886395096	37.0	35.0	37.0	32.0	37.0
8	35.7313923125059	37.0	35.0	37.0	35.0	37.0
9	37.68803222771918	39.0	37.0	39.0	35.0	39.0
10	37.34211690216253	39.0	37.0	39.0	34.0	39.0
11	37.32666851977878	39.0	37.0	39.0	34.0	39.0
12	37.324306486485746	39.0	37.0	39.0	34.0	39.0
13	37.30689179696646	39.0	37.0	39.0	34.0	39.0
14	38.55885844642566	40.0	38.0	41.0	34.0	41.0
15	38.69823758083973	40.0	38.0	41.0	35.0	41.0
16	38.60075201662872	40.0	38.0	41.0	35.0	41.0
17	38.5875848791529	40.0	38.0	41.0	34.0	41.0
18	38.46485225995237	40.0	38.0	41.0	34.0	41.0
19	38.30209926564727	40.0	37.0	41.0	34.0	41.0
20	38.31742851939537	40.0	37.0	41.0	34.0	41.0
21	38.224962789417546	40.0	37.0	41.0	34.0	41.0
22	38.285358269102005	40.0	37.0	41.0	34.0	41.0
23	38.28098611124424	40.0	37.0	41.0	34.0	41.0
24	38.248870674227	40.0	37.0	41.0	34.0	41.0
25	37.966025060830916	40.0	36.0	41.0	34.0	41.0
26	38.046838093229795	40.0	36.0	41.0	34.0	41.0
27	38.03669435836906	40.0	36.0	41.0	34.0	41.0
28	37.955836139246315	40.0	36.0	41.0	34.0	41.0
29	37.88181754696338	40.0	36.0	41.0	34.0	41.0
30	37.73157716728536	40.0	36.0	41.0	33.0	41.0
31	37.685919406054744	40.0	36.0	41.0	33.0	41.0
32	37.568099816103725	40.0	36.0	41.0	33.0	41.0
33	37.40367765160488	40.0	36.0	41.0	33.0	41.0
34	37.222747270653414	40.0	36.0	41.0	32.0	41.0
35	37.181391833252775	40.0	36.0	41.0	32.0	41.0
36	37.04644784425095	40.0	35.0	41.0	32.0	41.0
37	36.9729208287519	40.0	35.0	41.0	31.0	41.0
38	36.84994173651211	40.0	35.0	41.0	31.0	41.0
39	36.77790725219532	40.0	35.0	41.0	31.0	41.0
40	36.71220849428251	40.0	35.0	41.0	31.0	41.0
41	36.5965428048358	40.0	35.0	41.0	30.0	41.0
42	36.58040566723675	40.0	35.0	41.0	30.0	41.0
43	36.49133852661156	39.0	35.0	41.0	30.0	41.0
44	36.416902299456524	39.0	35.0	41.0	30.0	41.0
45	36.42133607557411	39.0	35.0	41.0	30.0	41.0
46	36.304258643330236	39.0	35.0	41.0	30.0	41.0
47	36.26655648408948	39.0	35.0	41.0	30.0	41.0
48	36.238614657203925	39.0	35.0	41.0	30.0	41.0
49	36.16090444651056	39.0	35.0	41.0	30.0	41.0
50	36.05138689009904	39.0	35.0	41.0	29.0	41.0
51	35.11129763947293	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	13.0
9	23.0
10	28.0
11	21.0
12	23.0
13	16.0
14	13.0
15	26.0
16	48.0
17	93.0
18	185.0
19	346.0
20	594.0
21	1023.0
22	1556.0
23	2393.0
24	3689.0
25	5619.0
26	7808.0
27	9023.0
28	9373.0
29	9552.0
30	10563.0
31	12946.0
32	16300.0
33	23272.0
34	38336.0
35	53672.0
36	54631.0
37	82977.0
38	140668.0
39	245259.0
40	213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.30052868466924	20.730984262696442	23.840926300453376	14.127560752180946
2	35.50663217869843	23.438490599107496	24.22090556933218	16.833971652861894
3	31.14364859517214	23.238984366762836	29.37109665440235	16.246270383662672
4	28.593474215496855	24.760270736940697	28.582519858195845	18.063735189366607
5	21.863527878154684	37.01573182637891	24.344005159502288	16.776735135964113
6	24.404527983590373	33.37422960059044	27.8054451371554	14.415797278663787
7	76.2991525435333	6.383788646630234	12.610793054389754	4.7062657554467116
8	77.03323141216728	6.264796940448006	12.013369793085884	4.688601854298832
9	70.05968755434388	9.237398723543516	14.712249573122389	5.990664148990214
10	36.07831270034493	29.1114783863684	21.38975192488597	13.420456988400705
11	26.743420196822417	26.108067473363793	28.820503270560298	18.32800905925349
12	25.504208527145583	22.77424575826746	32.699030402449395	19.022515312137564
13	23.471901388875576	23.890768626172974	33.31480221223246	19.322527772718995
14	19.52710039531537	27.292233497603053	32.849378956405765	20.331287150675813
15	19.21668129529798	26.21199693825713	35.482532592636204	19.088789173808678
16	21.52640753221608	26.145996935518546	33.04313415116739	19.284461381097984
17	21.48368553874214	25.73288073580418	31.162407932050122	21.621025793403557
18	22.125747806047627	25.49106329838437	31.92524198859925	20.45794690696875
19	22.79670219073453	27.010569585500814	30.41217138639715	19.780556837367502
20	23.41274785945012	26.270191961418753	31.434623710980237	18.88243646815089
21	22.780407584249275	27.247320632668902	31.484876825098624	18.487394957983195
22	21.946780993642363	25.03988070704899	31.197735734345883	21.815602564962763
23	20.81300501298776	26.443133877308462	31.489532426951556	21.254328682752227
24	20.75535770769119	25.084793571983138	32.87293082460294	21.286917895722734
25	20.278432376698436	27.155440960806683	30.291947315018557	22.274179347476323
26	19.47095931414769	27.596901560037406	31.175005442946286	21.757133682868616
27	20.2785693061647	26.840777047335145	31.814329120926523	21.06632452557363
28	19.14438253711131	26.67125836810201	33.02026693030153	21.16409216448515
29	19.855868043811952	25.856938832238125	32.38012167552372	21.9070714484262
30	20.674980110995026	26.015640083636516	32.42681462351928	20.882565181849177
31	21.656216666233057	27.191864198832537	29.559237741047212	21.592681393887194
32	21.463830766134055	27.369461716575177	29.684802061610043	21.481905455680725
33	21.273088019630208	26.680569571807865	30.645225337976157	21.40111707058577
34	20.146158512288732	27.238831005760623	30.65850749620363	21.95650298574701
35	20.108365979600247	26.41027080540543	30.153374695160778	23.327988519833546
36	21.301021630747783	27.8054451371554	30.160358097940172	20.733175134156646
37	21.45931209374739	27.945113192743285	30.31426681801937	20.281307895489952
38	20.176556853799042	28.515424419727154	29.274561380687192	22.033457345786612
39	22.302934535391476	26.189403576323798	29.782706629987825	21.724955258296898
40	20.989507095000295	26.344818520531888	30.66466932218545	22.001005062282367
41	19.26652362101758	26.15256954989915	30.415731552519983	24.16517527656329
42	21.539689690443556	25.470934666843764	29.59456554334297	23.394810099369714
43	21.674976003111038	25.842150449881764	30.24361121342785	22.239262333579347
44	20.513129481872593	26.16297618933511	29.818992938547424	23.50490139024487
45	20.604735294802296	26.346872462525827	29.17350743458537	23.87488480808651
46	20.877361862131195	27.110254236940012	29.55937467051347	22.453009230415322
47	19.8417643087869	26.02864838293147	31.439553171765695	22.690034136515937
48	20.070847305844286	24.858723023183526	31.81076895480369	23.259660716168497
49	21.19353199973162	25.089449173836066	31.280988849833562	22.436029976598753
50	19.130689590485044	25.532142138263158	31.444756491483673	23.892411779768125
51	19.175876314351715	25.006743776213437	30.2577149484529	25.559664960981948
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	335.0
1	878.0
2	1421.0
3	7815.0
4	14209.0
5	9126.5
6	4044.0
7	3908.5
8	3773.0
9	3676.5
10	3580.0
11	3526.5
12	3473.0
13	3319.0
14	3165.0
15	3035.5
16	2906.0
17	2891.0
18	2876.0
19	3101.5
20	3327.0
21	3748.5
22	4170.0
23	4130.0
24	4090.0
25	5125.0
26	7331.5
27	8503.0
28	9488.5
29	10474.0
30	13165.5
31	15857.0
32	17491.0
33	19125.0
34	20738.5
35	22352.0
36	24747.5
37	27143.0
38	29130.0
39	31117.0
40	33983.0
41	36849.0
42	40067.0
43	43285.0
44	47724.0
45	52163.0
46	56073.5
47	59984.0
48	63650.0
49	67316.0
50	64279.0
51	61242.0
52	53952.5
53	46663.0
54	41773.0
55	36883.0
56	33658.5
57	30434.0
58	28154.0
59	25874.0
60	24857.0
61	23840.0
62	21060.5
63	18281.0
64	15279.5
65	12278.0
66	10196.0
67	8114.0
68	6780.0
69	5446.0
70	4527.5
71	3609.0
72	3119.0
73	2629.0
74	2134.5
75	1262.0
76	884.0
77	624.5
78	365.0
79	280.0
80	195.0
81	175.0
82	155.0
83	105.0
84	55.0
85	30.0
86	5.0
87	6.0
88	7.0
89	5.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	730303.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.02819827864322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.8705076050645	25.875507873342208
2	10.426930559248142	7.304731821339761
3	3.773318136169659	3.9651760752645373
4	2.0439270233518894	2.86380324164188
5	1.2124849081641171	2.1235580886517598
6	0.8608068571140483	1.8091507962363944
7	0.7021481994229883	1.7216490445266526
8	0.5769822135747581	1.6168517904277673
9	0.4466273072018017	1.4080094885989085
>10	5.911791234681145	43.60234165682774
>50	0.12638577249325322	2.82357705772998
>100	0.04491066724829749	2.7727632339949624
>500	0.0015897581326830966	0.4033084846554905
>1k	0.0011923185995123225	0.5338930060938234
>5k	3.9743953317077416E-4	1.1756783406681455
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8445	1.1563693425879396	No Hit
GCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	1788	0.24482988567758862	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1042	0.14268050384566405	No Hit
CTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGCT	1005	0.13761411359394662	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCC	941	0.12885062775313808	No Hit
CCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	752	0.10297095862950036	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3692946626263345E-4	0.0	0.0	0.14418672797455304	0.0
2	1.3692946626263345E-4	0.0	0.0	0.6697220194905402	0.0
3	1.3692946626263345E-4	0.0	0.0	0.9491950601325752	0.0
4	1.3692946626263345E-4	0.0	0.0	1.2852199703410776	0.0
5	1.3692946626263345E-4	0.0	0.0	2.1922407548647618	0.0
6	1.3692946626263345E-4	0.0	0.0	2.9265934824312647	0.0
7	1.3692946626263345E-4	0.0	0.0	3.6037096930999875	0.0
8	1.3692946626263345E-4	0.0	0.0	4.6694317290220635	0.0
9	1.3692946626263345E-4	0.0	0.0	5.116232577437036	0.0
10	2.738589325252669E-4	0.0	0.0	6.064195272373248	0.0
11	2.738589325252669E-4	0.0	0.0	7.373514828776549	0.0
12	2.738589325252669E-4	0.0	0.0	8.397062589089733	0.0
13	4.1078839878790036E-4	0.0	0.0	8.871386260223495	0.0
14	4.1078839878790036E-4	0.0	0.0	9.078834401611386	0.0
15	5.477178650505338E-4	0.0	0.0	9.412531510893423	0.0
16	5.477178650505338E-4	0.0	0.0	10.031863486799315	0.0
17	5.477178650505338E-4	0.0	0.0	10.754851068666019	0.0
18	5.477178650505338E-4	0.0	0.0	11.59149010753071	0.0
19	5.477178650505338E-4	0.0	0.0	12.106481830144475	0.0
20	9.585062638384342E-4	0.0	0.0	12.627361519807533	0.0
21	9.585062638384342E-4	0.0	0.0	13.267232915652817	0.0
22	9.585062638384342E-4	0.0	0.0	13.930382320762751	0.0
23	9.585062638384342E-4	0.0	0.0	14.609826332357938	0.0
24	0.0010954357301010676	0.0	0.0	15.15425789021817	0.0
25	0.0010954357301010676	0.0	0.0	15.626253760425467	0.0
26	0.0010954357301010676	0.0	0.0	16.079490293754784	0.0
27	0.0010954357301010676	0.0	0.0	16.529303590427535	0.0
28	0.0010954357301010676	0.0	0.0	16.992399045327762	0.0
29	0.0010954357301010676	0.0	0.0	17.478635580026374	0.0
30	0.0010954357301010676	0.0	0.0	18.021013195892664	0.0
31	0.0010954357301010676	0.0	0.0	18.534772553310063	0.0
32	0.0010954357301010676	0.0	0.0	19.039768424886656	0.0
33	0.0010954357301010676	0.0	0.0	19.532029856100824	0.0
34	0.0012323651963637011	0.0	0.0	20.02798838290408	0.0
35	0.0012323651963637011	0.0	0.0	20.533531972345724	0.0
36	0.0013692946626263345	0.0	0.0	21.040855644848783	0.0
37	0.0013692946626263345	0.0	0.0	21.504361888147795	0.0
38	0.001506224128888968	0.0	0.0	22.02866481446742	0.0
39	0.001506224128888968	0.0	0.0	22.529142013657346	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATT	35	1.2111195E-7	45.000004	37
CATACGG	30	2.16441E-6	45.000004	2
AACCGGC	20	7.031596E-4	45.0	36
TCCGCGC	20	7.031596E-4	45.0	13
GATCGGT	20	7.031596E-4	45.0	23
CGTTCTA	20	7.031596E-4	45.0	31
CGTAGCG	25	3.889404E-5	45.0	2
TTACGAA	20	7.031596E-4	45.0	31
CCAACGG	20	7.031596E-4	45.0	2
TTCGACG	20	7.031596E-4	45.0	25
AAGTCGA	20	7.031596E-4	45.0	44
CGTTATT	450	0.0	43.5	1
CGTTTTT	3385	0.0	43.07238	1
CGTAAGG	85	0.0	42.35294	2
CGTATGG	80	0.0	42.1875	2
ACAACGG	60	3.6379788E-12	41.250004	2
TAAGGGA	630	0.0	41.07143	4
CCATCGA	50	1.0804797E-9	40.5	31
ATACGGA	40	3.4559162E-7	39.375	34
ATACGGG	220	0.0	38.863636	3
>>END_MODULE