FastQCFastQC Report
Sat 18 Jun 2016
SRR3551049_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551049_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835948
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230492.757228918545173No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTC117541.4060683200390456No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCG90261.079732232148411No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGC79810.9547244565451439No Hit
GCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC34940.41796858177781393No Hit
GAACTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCT33840.4048098685564174No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27690.3312406991822458No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCT20670.247264183896606No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTC16320.1952274543392651No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCC14570.1742931378506797No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAACACCGTCGTA14420.17249876786594381No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAACACCGT14260.17058477321555887No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTT13480.16125404929493223No Hit
CTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGCT12470.14917195806437722No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCG10570.1264432715910559No Hit
GAATATGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTC10450.12500777560326717No Hit
CGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTG9800.11723217233607833No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGC8840.1057482044337686No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA207.0321804E-445.00000424
CCGGGTC253.889889E-545.0000045
TCTTGCG207.0321804E-445.0000041
GGTCGAA207.0321804E-445.0000048
CCCGTCG406.8121153E-945.00000440
TATTACG253.889889E-545.0000041
GAACGTA207.0321804E-445.0000049
CGGTCTA207.0321804E-445.00000431
CCGTTGA207.0321804E-445.00000424
GGCTACG406.8121153E-945.0000041
CGTTTTT107600.044.2472151
CGTTATT13850.043.8628161
CCGATGA16150.042.77089718
TAATACG4750.042.6315774
ATTACGG800.042.1875042
CGATGAA16750.042.04477719
GCGAACC1250.041.39999833
AATACGG4950.041.3636365
GTCGAAT1800.041.25000443
GATTACG556.002665E-1140.9090921