##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551049_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	835948
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.919015297602243	33.0	31.0	34.0	30.0	34.0
2	32.095738012412255	33.0	31.0	34.0	30.0	34.0
3	31.8843492657438	33.0	31.0	34.0	30.0	34.0
4	35.656921243905124	37.0	35.0	37.0	33.0	37.0
5	31.871910693009614	37.0	35.0	37.0	0.0	37.0
6	33.71162799599975	37.0	35.0	37.0	17.0	37.0
7	35.37693851770684	37.0	35.0	37.0	32.0	37.0
8	35.58505433352314	37.0	35.0	37.0	33.0	37.0
9	37.572511687329836	39.0	37.0	39.0	35.0	39.0
10	37.301632398187444	39.0	37.0	39.0	34.0	39.0
11	37.28213836267328	39.0	37.0	39.0	34.0	39.0
12	37.294414245862185	39.0	37.0	39.0	34.0	39.0
13	37.2228607521042	39.0	37.0	39.0	34.0	39.0
14	38.54285912520874	40.0	38.0	41.0	34.0	41.0
15	38.58991468368846	40.0	38.0	41.0	34.0	41.0
16	38.619769411494495	40.0	38.0	41.0	34.0	41.0
17	38.49777139247896	40.0	38.0	41.0	34.0	41.0
18	38.27889773048084	40.0	38.0	41.0	34.0	41.0
19	37.9683820046223	40.0	37.0	41.0	34.0	41.0
20	37.61403340877662	39.0	35.0	41.0	33.0	41.0
21	37.68286903013106	39.0	35.0	41.0	33.0	41.0
22	37.710071679099656	39.0	35.0	41.0	33.0	41.0
23	37.62767899438721	39.0	35.0	41.0	33.0	41.0
24	37.60593123017221	39.0	35.0	41.0	33.0	41.0
25	37.47829769315795	39.0	35.0	41.0	33.0	41.0
26	37.39115471297258	39.0	35.0	41.0	33.0	41.0
27	37.31828295539914	39.0	35.0	41.0	33.0	41.0
28	37.25349902147023	39.0	35.0	41.0	33.0	41.0
29	37.159585285209126	39.0	35.0	41.0	33.0	41.0
30	36.98750161493299	39.0	35.0	41.0	33.0	41.0
31	36.773749084871305	39.0	35.0	41.0	31.0	41.0
32	36.27151210362367	39.0	35.0	41.0	30.0	41.0
33	35.79950188289224	39.0	35.0	41.0	25.0	41.0
34	35.32095178168977	39.0	35.0	41.0	22.0	41.0
35	35.006130764114516	39.0	35.0	41.0	19.0	41.0
36	34.82700359352496	39.0	35.0	41.0	18.0	41.0
37	34.63194480996425	39.0	35.0	41.0	16.0	41.0
38	34.49438721068775	39.0	35.0	41.0	15.0	41.0
39	34.3290396053343	39.0	34.0	41.0	15.0	41.0
40	34.27881518946155	39.0	34.0	41.0	15.0	41.0
41	34.166639551742456	38.0	34.0	41.0	15.0	41.0
42	34.12035437610952	38.0	34.0	41.0	15.0	41.0
43	33.98441290606593	38.0	34.0	41.0	12.0	41.0
44	33.99680841392048	38.0	34.0	41.0	12.0	41.0
45	34.037990401316826	38.0	34.0	41.0	13.0	41.0
46	33.858031839301006	38.0	34.0	41.0	12.0	41.0
47	33.731418700684735	38.0	33.0	40.0	12.0	41.0
48	33.71293668984195	38.0	33.0	40.0	12.0	41.0
49	33.70903094450851	38.0	33.0	40.0	12.0	41.0
50	33.58740376195649	38.0	33.0	40.0	11.0	41.0
51	32.736111576318145	37.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	25.0
10	20.0
11	13.0
12	13.0
13	13.0
14	17.0
15	30.0
16	78.0
17	152.0
18	349.0
19	832.0
20	1829.0
21	3322.0
22	5224.0
23	7638.0
24	10682.0
25	15474.0
26	21137.0
27	21948.0
28	19738.0
29	16838.0
30	16310.0
31	17897.0
32	21558.0
33	27920.0
34	41100.0
35	57578.0
36	61021.0
37	85984.0
38	141546.0
39	239461.0
40	188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.69541167632437	17.25896826118371	20.722341581055282	22.323278481436645
2	39.27540947523052	27.09091953087991	19.60564532722131	14.028025666668261
3	25.564269547866616	27.086613042916547	34.25236976462651	13.096747644590334
4	22.568269796685918	20.577117236957324	41.434036566867796	15.420576399488963
5	23.05406556388675	32.2375315210994	30.253317191978446	14.455085723035404
6	19.976122916736447	33.587615497614685	33.99840659945356	12.437854986195314
7	64.54588084426304	4.896357189681655	26.515764138439234	4.041997827616072
8	65.03275323345471	9.699407140157042	20.99209520209391	4.275744424294333
9	60.141898778392914	6.6195504983563564	22.02481494064224	11.213735782608488
10	32.837329594663785	25.818471962370865	28.687430318632263	12.656768124333093
11	25.265566757740913	21.617134080110244	36.60598506127176	16.51131410087709
12	21.11315536373076	18.74351036188854	39.51250556254695	20.63082871183375
13	21.53255944149636	20.594582438142087	41.969835444309936	15.90302267605162
14	16.89016541698766	26.89150521324293	37.876279385799116	18.342049983970295
15	15.266978328795572	23.076315751697475	43.118112609875254	18.538593309631697
16	17.319498342002134	23.583643958715136	37.6241105906109	21.47274710867183
17	17.416274696512225	25.12979276222923	38.18634651916148	19.26758602209707
18	17.534224616842195	23.34403575342007	38.3234363859953	20.79830324374243
19	17.80433711187777	24.712183054448364	36.32678109164685	21.156698742027018
20	20.8218693028753	24.00400503380593	38.88914142984971	16.284984233469068
21	18.57448070932642	27.483288434208824	37.950685927832836	15.991544928631923
22	17.80338011455258	21.756975314253996	39.58679247991502	20.852852091278404
23	18.78442199754052	24.350677314856906	38.26039418719825	18.60450650040433
24	18.074808480910296	24.65117447496734	37.20578313483614	20.068233909286224
25	16.674721394153703	29.364505926205936	35.316790039571835	18.643982640068522
26	16.502461875619055	24.89915640685784	38.75731504830444	19.84106666921866
27	20.280089192150708	25.60769330149722	35.64874848674798	18.46346901960409
28	15.276787551378792	27.52001320656309	38.30728705613268	18.89591218592544
29	17.52872188222234	24.873078229746348	37.94901118251374	19.649188705517567
30	16.337499461689003	30.08656040806366	35.95522688014087	17.620713250106466
31	17.919655289563465	26.856215936876453	33.0199964591099	22.20413231445018
32	20.07588988788776	27.42311722738735	32.35811318407365	20.142879700651235
33	19.10334135616091	27.38746907702393	31.838463636494136	21.670725930321026
34	17.297726652854006	27.206118083899955	31.637972696866314	23.858182566379725
35	19.014938728246257	26.90274993181394	30.44627177767038	23.636039562269424
36	19.217224037858816	31.61141602109222	29.74766373027987	19.42369621076909
37	17.877427782589347	28.25283390832923	32.08847918770067	21.781259121380756
38	17.857211214094658	29.25839884777522	29.65160512376368	23.232784814366443
39	19.830300449310243	26.19540928383105	30.49053290396053	23.48375736289817
40	18.307478455597717	26.824635025145106	32.07663634580141	22.791250173455765
41	18.45736816165599	27.12034719862958	30.106896601223998	24.315388038490433
42	19.088866771617376	25.93020140008709	32.37354476594238	22.60738706235316
43	21.57993080909339	26.502605425217833	30.40356577203367	21.513897993655107
44	19.554326345657863	26.231177058860123	31.44764985381866	22.766846741663358
45	17.992267461612446	25.552307081301706	31.340944652059697	25.11448080502615
46	21.432912095010696	27.459483125744665	29.878413489834294	21.229191289410345
47	17.416872819840467	26.569595237981307	34.77871829348237	21.23481364869585
48	18.828204625168073	26.12184011445688	31.51727140922641	23.532683851148636
49	18.621732452257795	24.150425624560377	34.57906472651409	22.648777196667737
50	18.647691004703642	24.595190131443584	33.19871570958959	23.558403154263182
51	17.95733705924292	24.225310665256693	31.406977467497978	26.410374808002413
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	460.0
1	819.0
2	1178.0
3	19978.0
4	38778.0
5	25278.5
6	11779.0
7	11330.0
8	10881.0
9	10720.0
10	10559.0
11	10209.0
12	9859.0
13	9513.0
14	9167.0
15	8613.0
16	8059.0
17	7735.0
18	7411.0
19	7065.5
20	6720.0
21	6702.0
22	6684.0
23	6729.5
24	6775.0
25	7229.0
26	8409.5
27	9136.0
28	10851.0
29	12566.0
30	13798.0
31	15030.0
32	16582.0
33	18134.0
34	20441.0
35	22748.0
36	24845.0
37	26942.0
38	28683.5
39	30425.0
40	33308.0
41	36191.0
42	40042.5
43	43894.0
44	48787.5
45	53681.0
46	75970.0
47	98259.0
48	83727.0
49	69195.0
50	66416.0
51	63637.0
52	55433.0
53	47229.0
54	41438.0
55	35647.0
56	31782.0
57	27917.0
58	25784.5
59	23652.0
60	21926.0
61	20200.0
62	17887.5
63	15575.0
64	13074.5
65	10574.0
66	8568.5
67	6563.0
68	5409.5
69	4256.0
70	3691.0
71	3126.0
72	2682.0
73	2238.0
74	1800.0
75	1166.5
76	971.0
77	675.0
78	379.0
79	308.0
80	237.0
81	172.5
82	108.0
83	68.5
84	29.0
85	28.5
86	28.0
87	18.5
88	9.0
89	12.0
90	15.0
91	8.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	835948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.63466088610912
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.752974543709	29.262068298387632
2	8.762589815658005	6.5955419239272475
3	3.3332373723522184	3.763357744841435
4	1.7903735789426205	2.6952041001182017
5	1.1688130928522742	2.1993942194369853
6	0.8820142710229083	1.9916584791993537
7	0.6674963567211538	1.758469931953976
8	0.5302533043932661	1.596472263566349
9	0.45458038070119106	1.5397180625850937
>10	4.513317415110138	34.06741622043355
>50	0.10834278497478372	2.5848815319484295
>100	0.027326804489427283	2.0813104958919677
>500	0.003536409992749111	0.8895357961746925
>1k	0.003857901810271758	2.706357003378063
>5k	6.429836350452929E-4	2.057716986608133
>10k+	6.429836350452929E-4	4.210896941548942
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23049	2.757228918545173	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTC	11754	1.4060683200390456	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCG	9026	1.079732232148411	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGC	7981	0.9547244565451439	No Hit
GCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	3494	0.41796858177781393	No Hit
GAACTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCT	3384	0.4048098685564174	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2769	0.3312406991822458	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCT	2067	0.247264183896606	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTC	1632	0.1952274543392651	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCC	1457	0.1742931378506797	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAACACCGTCGTA	1442	0.17249876786594381	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAACACCGT	1426	0.17058477321555887	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTT	1348	0.16125404929493223	No Hit
CTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGCT	1247	0.14917195806437722	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCG	1057	0.1264432715910559	No Hit
GAATATGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTC	1045	0.12500777560326717	No Hit
CGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTG	980	0.11723217233607833	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGC	884	0.1057482044337686	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.588739969471785E-4	0.0	0.0	0.15730643532851327	0.0
2	3.588739969471785E-4	0.0	0.0	0.7873695493021097	0.0
3	4.78498662596238E-4	0.0	0.0	1.0756649935163431	0.0
4	4.78498662596238E-4	0.0	0.0	1.7643441936579787	0.0
5	4.78498662596238E-4	0.0	0.0	3.8598094618325542	0.0
6	4.78498662596238E-4	0.0	0.0	4.761420566829516	0.0
7	4.78498662596238E-4	0.0	0.0	5.659203682525708	0.0
8	4.78498662596238E-4	0.0	0.0	6.729964064750439	0.0
9	4.78498662596238E-4	0.0	0.0	7.151282137166427	0.0
10	4.78498662596238E-4	0.0	0.0	9.138128208931656	0.0
11	4.78498662596238E-4	0.0	0.0	10.25446558876868	0.0
12	4.78498662596238E-4	0.0	0.0	12.191906673620847	0.0
13	4.78498662596238E-4	0.0	0.0	12.679377186140766	0.0
14	5.981233282452976E-4	0.0	0.0	12.902237938244962	0.0
15	5.981233282452976E-4	0.0	0.0	13.343533329824343	0.0
16	5.981233282452976E-4	0.0	0.0	13.872872475321431	0.0
17	5.981233282452976E-4	0.0	0.0	14.467287438931608	0.0
18	5.981233282452976E-4	0.0	0.0	15.11314100877088	0.0
19	5.981233282452976E-4	0.0	0.0	15.745118117394862	0.0
20	5.981233282452976E-4	0.0	0.0	16.174690291740635	0.0
21	5.981233282452976E-4	0.0	0.0	16.642781608425405	0.0
22	5.981233282452976E-4	0.0	0.0	17.212314641580576	0.0
23	5.981233282452976E-4	0.0	0.0	17.71031212467761	0.0
24	5.981233282452976E-4	0.0	0.0	18.10854263662333	0.0
25	5.981233282452976E-4	0.0	0.0	18.48045572212626	0.0
26	5.981233282452976E-4	0.0	0.0	18.82736725250853	0.0
27	5.981233282452976E-4	0.0	0.0	19.19114586074732	0.0
28	7.17747993894357E-4	0.0	0.0	19.54332087641815	0.0
29	7.17747993894357E-4	0.0	0.0	19.93521128108447	0.0
30	7.17747993894357E-4	0.0	0.0	20.38129165928981	0.0
31	8.373726595434166E-4	0.0	0.0	20.771148444640097	0.0
32	8.373726595434166E-4	0.0	0.0	21.1461717714499	0.0
33	9.56997325192476E-4	0.0	0.0	21.501696277758903	0.0
34	0.0010766219908415356	0.0	0.0	21.843822821515214	0.0
35	0.0010766219908415356	0.0	0.0	22.21824802499677	0.0
36	0.0010766219908415356	0.0	0.0	22.5831032552264	0.0
37	0.0010766219908415356	0.0	0.0	22.942216501504877	0.0
38	0.0011962466564905952	0.0	0.0	23.305157737084127	0.0
39	0.0013158713221396547	0.0	0.0	23.66462985735955	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	20	7.0321804E-4	45.000004	24
CCGGGTC	25	3.889889E-5	45.000004	5
TCTTGCG	20	7.0321804E-4	45.000004	1
GGTCGAA	20	7.0321804E-4	45.000004	8
CCCGTCG	40	6.8121153E-9	45.000004	40
TATTACG	25	3.889889E-5	45.000004	1
GAACGTA	20	7.0321804E-4	45.000004	9
CGGTCTA	20	7.0321804E-4	45.000004	31
CCGTTGA	20	7.0321804E-4	45.000004	24
GGCTACG	40	6.8121153E-9	45.000004	1
CGTTTTT	10760	0.0	44.247215	1
CGTTATT	1385	0.0	43.862816	1
CCGATGA	1615	0.0	42.770897	18
TAATACG	475	0.0	42.631577	4
ATTACGG	80	0.0	42.187504	2
CGATGAA	1675	0.0	42.044777	19
GCGAACC	125	0.0	41.399998	33
AATACGG	495	0.0	41.363636	5
GTCGAAT	180	0.0	41.250004	43
GATTACG	55	6.002665E-11	40.909092	1
>>END_MODULE