FastQCFastQC Report
Sat 18 Jun 2016
SRR3551048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627538
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94821.5109841953794034No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11520.1835745405059136No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC10570.1684360150301655TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC7960.12684490819679448No Hit
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT7900.12588879079832616Illumina PCR Primer Index 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG351.2107557E-745.0000041
TAGCGCG351.2107557E-745.0000041
GTAACGT302.163919E-645.00000436
ACGTTGC207.030836E-445.044
CGATTCA207.030836E-445.010
TCTAGCG253.8887745E-545.01
TAGCACG253.8887745E-545.01
CGCACGG406.8066583E-945.02
GTAAGCG253.8887745E-545.01
CCAGTCG207.030836E-445.028
TCGACTA207.030836E-445.024
GCGTATG253.8887745E-545.01
ACCACGG551.8189894E-1244.9999962
CGTTATT7300.044.3835641
CGTTTTT50300.043.4791261
TACGAAG650.041.5384641
GACACGA1600.040.7812525
CACGACC1550.040.6451627
GTACGAG501.0804797E-940.51
ATGAGCG403.4548793E-739.3751
Produced by FastQC (version 0.11.5)