##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551043_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	626667
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.011516483235916	33.0	31.0	34.0	30.0	34.0
2	32.11734940566521	33.0	31.0	34.0	30.0	34.0
3	32.05743082051552	33.0	31.0	34.0	30.0	34.0
4	35.71139855776673	37.0	35.0	37.0	33.0	37.0
5	31.93832928812272	37.0	35.0	37.0	0.0	37.0
6	33.76565863528796	37.0	35.0	37.0	17.0	37.0
7	35.41758222469031	37.0	35.0	37.0	32.0	37.0
8	35.64536827374028	37.0	35.0	37.0	33.0	37.0
9	37.56704278348788	39.0	37.0	39.0	35.0	39.0
10	37.2765392146068	39.0	37.0	39.0	34.0	39.0
11	37.2353051939866	39.0	37.0	39.0	34.0	39.0
12	37.1151632366153	39.0	37.0	39.0	34.0	39.0
13	36.986865432518385	39.0	37.0	39.0	33.0	39.0
14	38.18463873157514	40.0	37.0	41.0	33.0	41.0
15	38.3071535600247	40.0	37.0	41.0	34.0	41.0
16	38.318346107262705	40.0	37.0	41.0	34.0	41.0
17	38.26065358475873	40.0	37.0	41.0	34.0	41.0
18	38.166445656145925	40.0	37.0	41.0	34.0	41.0
19	38.026963283530165	40.0	37.0	41.0	34.0	41.0
20	37.98746702794307	40.0	36.0	41.0	34.0	41.0
21	37.94209683931019	40.0	36.0	41.0	34.0	41.0
22	38.001488828995306	40.0	36.0	41.0	34.0	41.0
23	38.00192765854912	40.0	36.0	41.0	34.0	41.0
24	37.98755958108533	40.0	36.0	41.0	34.0	41.0
25	37.73324588657134	40.0	35.0	41.0	33.0	41.0
26	37.75359162042999	40.0	35.0	41.0	33.0	41.0
27	37.71156451512526	40.0	35.0	41.0	33.0	41.0
28	37.59146564283743	40.0	35.0	41.0	33.0	41.0
29	37.53151354706726	40.0	35.0	41.0	33.0	41.0
30	37.363574274694535	40.0	35.0	41.0	33.0	41.0
31	37.34959875021343	40.0	35.0	41.0	33.0	41.0
32	37.23275200385532	40.0	35.0	41.0	33.0	41.0
33	37.08927229294027	40.0	35.0	41.0	32.0	41.0
34	36.959919702170374	40.0	35.0	41.0	32.0	41.0
35	36.89286495060375	40.0	35.0	41.0	31.0	41.0
36	36.766657570926824	40.0	35.0	41.0	31.0	41.0
37	36.715754938428226	40.0	35.0	41.0	31.0	41.0
38	36.56101246754656	39.0	35.0	41.0	31.0	41.0
39	36.49313750365027	39.0	35.0	41.0	30.0	41.0
40	36.3997529788548	39.0	35.0	41.0	30.0	41.0
41	36.29115303662073	39.0	35.0	41.0	30.0	41.0
42	36.29679399106703	39.0	35.0	41.0	30.0	41.0
43	36.20434616790097	39.0	35.0	41.0	30.0	41.0
44	36.187807878825595	39.0	35.0	41.0	30.0	41.0
45	36.1959780872457	39.0	35.0	41.0	30.0	41.0
46	36.052685078359	39.0	35.0	41.0	30.0	41.0
47	35.97323778019267	39.0	35.0	41.0	30.0	41.0
48	35.94289630697005	39.0	35.0	41.0	30.0	41.0
49	35.890249526463016	39.0	35.0	41.0	29.0	41.0
50	35.7621256584438	38.0	35.0	41.0	29.0	41.0
51	34.808111804195846	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	11.0
9	20.0
10	36.0
11	25.0
12	14.0
13	13.0
14	16.0
15	26.0
16	51.0
17	62.0
18	184.0
19	347.0
20	647.0
21	1262.0
22	1845.0
23	2867.0
24	3776.0
25	5133.0
26	6553.0
27	7562.0
28	7970.0
29	8443.0
30	9965.0
31	12363.0
32	16017.0
33	23117.0
34	39052.0
35	54026.0
36	46727.0
37	70463.0
38	113145.0
39	194763.0
40	162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.87981655328907	18.85483039636681	21.882116020151052	14.383237030193069
2	32.74115279725915	26.228283921125573	23.556370448739123	17.474192832876152
3	30.263760497999737	26.08179463734328	27.140091946759604	16.514352917897384
4	27.7108895154843	23.466849219761055	30.213175418523715	18.609085846230933
5	23.99982765966614	35.96264044540402	22.526636953916515	17.51089494101333
6	24.09716803342126	34.866683581551285	26.221422222647757	14.814726162379701
7	74.65879007511165	6.3007945208539775	14.268981771818206	4.771433632216153
8	74.67267304645051	8.344623220945095	11.477866235177535	5.504837497426863
9	68.27182538732693	8.366484911444196	13.880577723096957	9.481111978131926
10	40.24976582459265	26.202592445429552	19.69802143722264	13.849620292755164
11	31.205089784526713	24.356316831746366	26.43253913162812	18.006054252098803
12	29.011261164222784	20.421850839441046	31.070249430718388	19.496638565617786
13	25.3150397260427	23.685625699135265	32.443386998198406	18.55594757662363
14	19.462968370761505	29.39519713021429	29.317005682443785	21.824828816580418
15	17.645894869204856	24.853231461047095	38.09630952323961	19.40456414650843
16	20.60871244217423	23.873763896934097	33.12030153175451	22.397222129137166
17	20.515201853616034	24.029987218091904	30.925994188300965	24.528816739991097
18	22.3452008802123	23.682912934620777	31.28088761654914	22.690998568617783
19	23.445944975561183	25.597645958698955	28.12801695318247	22.828392112557385
20	25.655571459802417	24.472327408336486	30.169292463142305	19.702808668718795
21	23.73748737367693	27.104506859304863	29.013176056821244	20.144829710196962
22	21.19387170538739	24.96732714503875	29.035516470469965	24.803284679103893
23	21.39669074644109	25.97089044101572	29.99344149285027	22.638977319692916
24	22.119562702360266	23.582381073201557	30.64354753002791	23.65450869441027
25	20.300255159438745	26.868655920927704	28.727537910884088	24.103551008749463
26	19.57163852572419	26.319400893935697	29.83083519636426	24.27812538397586
27	22.145732901205903	25.889507505581115	29.554611939036203	22.41014765417678
28	18.78717085788784	26.553177365331187	32.16780203840317	22.491849738377798
29	20.935680353361512	23.682434211471165	31.799025638816147	23.582859796351173
30	20.65068050495718	26.325145571731078	31.211472759854914	21.812701163456826
31	21.623924668125177	24.918656958161193	29.144505774199057	24.312912599514576
32	22.89924313870046	25.55424172646717	29.474026875517616	22.072488259314756
33	22.214988183516923	25.85184795114471	29.11115472810919	22.82200913722918
34	19.70025547858751	24.984561178424904	29.709079941978757	25.606103401008827
35	20.282382828519772	24.105465901347927	29.36296310480686	26.249188165325442
36	20.22589349686516	26.79716659725181	30.052164865869752	22.924775040013277
37	19.335149289814208	26.568975229268492	32.304238136043544	21.791637344873752
38	19.70232994556918	26.368230655196463	29.074293045588806	24.85514635364556
39	21.529297058884545	24.47711463983264	30.314345577475756	23.67924272380706
40	21.5621693818248	24.201210531270995	29.619239564234274	24.617380522669936
41	17.939990457451884	24.506635900725584	29.757750128856316	27.795623512966216
42	19.79600010851058	24.23504030051048	30.305090263249863	25.66386932772908
43	21.39972265972199	24.63684859742096	30.216686054954227	23.746742687902824
44	20.62068052091462	24.267912623450734	30.12923929295782	24.98216756267683
45	19.821532009823397	24.141210563185872	28.598282660487946	27.43897476650278
46	21.38424394455109	26.504985901603245	27.689506548134812	24.42126360571085
47	18.905415475842833	24.04817869777729	32.77769533101312	24.268710495366758
48	19.85807454357737	23.197328086527612	30.73945173433418	26.205145635560832
49	19.758180979690966	22.582168839271894	32.91588674686875	24.743763434168386
50	19.676000172340334	23.003923934083016	31.5478555596513	25.772220333925354
51	18.810628292218993	23.243285508890686	29.222218498819945	28.72386770007037
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	438.0
1	783.0
2	1128.0
3	4722.0
4	8316.0
5	5517.0
6	2718.0
7	2582.0
8	2446.0
9	2425.0
10	2404.0
11	2314.0
12	2224.0
13	2298.5
14	2373.0
15	2195.5
16	2018.0
17	2016.5
18	2015.0
19	2044.0
20	2073.0
21	2380.5
22	2688.0
23	2747.5
24	2807.0
25	3291.0
26	4344.0
27	4913.0
28	5694.0
29	6475.0
30	7918.0
31	9361.0
32	11008.5
33	12656.0
34	14104.5
35	15553.0
36	16425.5
37	17298.0
38	19784.0
39	22270.0
40	25447.5
41	28625.0
42	34252.0
43	39879.0
44	51903.0
45	63927.0
46	61315.0
47	58703.0
48	57722.0
49	56741.0
50	49022.0
51	41303.0
52	37873.0
53	34443.0
54	32289.5
55	30136.0
56	29428.0
57	28720.0
58	28123.5
59	27527.0
60	27432.5
61	27338.0
62	24471.0
63	21604.0
64	18330.0
65	15056.0
66	12187.0
67	9318.0
68	7905.0
69	6492.0
70	5317.0
71	4142.0
72	3331.5
73	2521.0
74	2207.5
75	1528.0
76	1162.0
77	876.0
78	590.0
79	492.0
80	394.0
81	248.5
82	103.0
83	73.5
84	44.0
85	41.5
86	39.0
87	26.0
88	13.0
89	8.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	626667.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.946317953143655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.42218808345211	22.721463772460243
2	10.553525942099073	6.531855386618958
3	3.892830043256575	3.614062687685038
4	2.1281194330241227	2.634298424665132
5	1.3098785218746565	2.0267958608963474
6	0.8934598145341051	1.6589574899357509
7	0.654262222950921	1.4172904743318833
8	0.5023893351106116	1.2437680080481137
9	0.41876066644811644	1.1663190657156373
>10	5.794438190024746	42.87355130252326
>50	0.36727596931145323	6.806208078514329
>100	0.05448887419169959	2.9977602830917034
>500	0.004715383343512464	0.9760677648446132
>1k	0.0036675203782874726	3.331601400668956
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4531	0.7230315303023774	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC	3934	0.6277656235289236	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCG	3812	0.6082975487778997	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGC	3367	0.5372869482516233	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	2607	0.41601041701573566	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1292	0.206170103101009	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	1005	0.1603722551211409	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCT	912	0.14553183748306517	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT	774	0.12351057260075926	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT	770	0.1228722750679388	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	672	0.10723398551383749	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	672	0.10723398551383749	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5957438320511532E-4	0.0	0.0	0.16659565606614038	0.0
2	1.5957438320511532E-4	0.0	0.0	1.001807977761714	0.0
3	1.5957438320511532E-4	0.0	0.0	1.2957439916255364	0.0
4	1.5957438320511532E-4	0.0	0.0	1.774307566857677	0.0
5	1.5957438320511532E-4	0.0	0.0	3.4003705317178023	0.0
6	1.5957438320511532E-4	0.0	0.0	4.30994451598696	0.0
7	1.5957438320511532E-4	0.0	0.0	5.166061081882403	0.0
8	1.5957438320511532E-4	0.0	0.0	6.32425195518513	0.0
9	1.5957438320511532E-4	0.0	0.0	6.731166632358175	0.0
10	1.5957438320511532E-4	0.0	0.0	8.324995571810867	0.0
11	1.5957438320511532E-4	0.0	0.0	9.588505538028969	0.0
12	1.5957438320511532E-4	0.0	0.0	11.197334469502941	0.0
13	1.5957438320511532E-4	0.0	0.0	11.730632058174438	0.0
14	1.5957438320511532E-4	0.0	0.0	11.97781277775916	0.0
15	1.5957438320511532E-4	0.0	0.0	12.364142359498745	0.0
16	1.5957438320511532E-4	0.0	0.0	13.0428122112701	0.0
17	1.5957438320511532E-4	0.0	0.0	13.791694791651707	0.0
18	1.5957438320511532E-4	0.0	0.0	14.643343274817408	0.0
19	1.5957438320511532E-4	0.0	0.0	15.209672760812362	0.0
20	1.5957438320511532E-4	0.0	0.0	15.698608670952835	0.0
21	1.5957438320511532E-4	0.0	0.0	16.275470066239325	0.0
22	1.5957438320511532E-4	0.0	0.0	16.917437809873505	0.0
23	1.5957438320511532E-4	0.0	0.0	17.530043866997943	0.0
24	1.5957438320511532E-4	0.0	0.0	17.99201170637675	0.0
25	1.5957438320511532E-4	0.0	0.0	18.39318170575441	0.0
26	1.5957438320511532E-4	0.0	0.0	18.80647935825566	0.0
27	1.5957438320511532E-4	0.0	0.0	19.211638717213447	0.0
28	1.5957438320511532E-4	0.0	0.0	19.638500192287133	0.0
29	1.5957438320511532E-4	0.0	0.0	20.07334038652107	0.0
30	1.5957438320511532E-4	0.0	0.0	20.570733738971416	0.0
31	1.5957438320511532E-4	0.0	0.0	21.04004199997766	0.0
32	1.5957438320511532E-4	0.0	0.0	21.501531116206852	0.0
33	1.5957438320511532E-4	0.0	0.0	21.940201095637715	0.0
34	3.1914876641023064E-4	0.0	0.0	22.37583916178768	0.0
35	3.1914876641023064E-4	0.0	0.0	22.831743174604693	0.0
36	3.1914876641023064E-4	0.0	0.0	23.26738124075466	0.0
37	4.7872314961534595E-4	0.0	0.0	23.70190228622219	0.0
38	4.7872314961534595E-4	0.0	0.0	24.145678645915613	0.0
39	6.382975328204613E-4	0.0	0.0	24.600306063666988	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTACG	20	7.030827E-4	45.000004	18
CGGGTAC	40	6.8066583E-9	45.000004	6
ATCTCGC	30	2.1639153E-6	45.000004	25
CGCGAGG	40	6.8066583E-9	45.000004	2
CCCGTAG	20	7.030827E-4	45.000004	1
TCTACGT	20	7.030827E-4	45.000004	16
TATAGCG	20	7.030827E-4	45.000004	1
TCGAGTG	20	7.030827E-4	45.000004	1
GACGATT	20	7.030827E-4	45.000004	33
TCGAATG	40	6.8066583E-9	45.000004	44
ACGGTTT	20	7.030827E-4	45.000004	5
GCCTATC	25	3.8887687E-5	45.0	18
GCTACGA	150	0.0	42.0	10
CGATGAA	355	0.0	41.830986	19
CGAAAGG	60	3.6379788E-12	41.250004	2
ACGGGAT	215	0.0	40.813957	5
TAATACG	155	0.0	40.645164	4
CGAATAT	155	0.0	40.645164	14
CATACGA	155	0.0	40.645164	18
ACATACG	155	0.0	40.645164	17
>>END_MODULE