##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551041_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	688027
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09475645577862	33.0	31.0	34.0	30.0	34.0
2	32.21602640594046	33.0	31.0	34.0	30.0	34.0
3	32.15208996158581	33.0	31.0	34.0	30.0	34.0
4	35.789146356174975	37.0	35.0	37.0	35.0	37.0
5	31.983763718575	37.0	35.0	37.0	0.0	37.0
6	33.835949751971945	37.0	35.0	37.0	17.0	37.0
7	35.54637390683796	37.0	35.0	37.0	32.0	37.0
8	35.80247577493325	37.0	35.0	37.0	35.0	37.0
9	37.78838330472496	39.0	38.0	39.0	35.0	39.0
10	37.36976165179564	39.0	37.0	39.0	34.0	39.0
11	37.298649616948175	39.0	37.0	39.0	34.0	39.0
12	37.270173990264915	39.0	37.0	39.0	34.0	39.0
13	37.23628578529622	39.0	37.0	39.0	34.0	39.0
14	38.46848597511435	40.0	38.0	41.0	34.0	41.0
15	38.619942240638814	40.0	38.0	41.0	35.0	41.0
16	38.55044496800271	40.0	38.0	41.0	34.0	41.0
17	38.54328100496056	40.0	38.0	41.0	34.0	41.0
18	38.44708855902457	40.0	38.0	41.0	34.0	41.0
19	38.27533803179236	40.0	37.0	41.0	34.0	41.0
20	38.310107016149075	40.0	37.0	41.0	34.0	41.0
21	38.23885835875627	40.0	37.0	41.0	34.0	41.0
22	38.29580815869144	40.0	37.0	41.0	34.0	41.0
23	38.30552289372365	40.0	37.0	41.0	34.0	41.0
24	38.27581766413237	40.0	37.0	41.0	34.0	41.0
25	38.01025541148821	40.0	36.0	41.0	34.0	41.0
26	38.05355167747777	40.0	36.0	41.0	34.0	41.0
27	38.03635177107875	40.0	36.0	41.0	34.0	41.0
28	37.972908039946105	40.0	36.0	41.0	34.0	41.0
29	37.938643396262066	40.0	36.0	41.0	34.0	41.0
30	37.76197155053508	40.0	36.0	41.0	33.0	41.0
31	37.716704431657476	40.0	36.0	41.0	33.0	41.0
32	37.60413472145715	40.0	36.0	41.0	33.0	41.0
33	37.48829624418809	40.0	36.0	41.0	33.0	41.0
34	37.33464239048758	40.0	36.0	41.0	33.0	41.0
35	37.28276215904318	40.0	36.0	41.0	33.0	41.0
36	37.15044467731644	40.0	35.0	41.0	32.0	41.0
37	37.07939368658497	40.0	35.0	41.0	32.0	41.0
38	36.97158396400141	40.0	35.0	41.0	32.0	41.0
39	36.88316301540492	40.0	35.0	41.0	31.0	41.0
40	36.815238355471514	40.0	35.0	41.0	31.0	41.0
41	36.68587569964551	39.0	35.0	41.0	31.0	41.0
42	36.67040973682719	39.0	35.0	41.0	31.0	41.0
43	36.599456125995054	39.0	35.0	41.0	31.0	41.0
44	36.529176907301604	39.0	35.0	41.0	31.0	41.0
45	36.48853460692676	39.0	35.0	41.0	31.0	41.0
46	36.36356858088418	39.0	35.0	41.0	30.0	41.0
47	36.274657825928344	39.0	35.0	41.0	30.0	41.0
48	36.21980242054454	39.0	35.0	41.0	30.0	41.0
49	36.146896851431705	39.0	35.0	41.0	30.0	41.0
50	36.02032187690309	39.0	35.0	41.0	30.0	41.0
51	35.02390603857116	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	23.0
9	26.0
10	15.0
11	22.0
12	21.0
13	13.0
14	17.0
15	17.0
16	41.0
17	77.0
18	172.0
19	323.0
20	562.0
21	1006.0
22	1547.0
23	2257.0
24	3341.0
25	4825.0
26	6453.0
27	7367.0
28	7707.0
29	8197.0
30	9480.0
31	11786.0
32	15430.0
33	22805.0
34	38979.0
35	52962.0
36	51155.0
37	80593.0
38	133821.0
39	226819.0
40	166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.9808713902216	19.636438686272488	24.01373783296295	13.368952090542956
2	33.30552434715498	24.067514792297395	25.7482627861988	16.878698074348826
3	32.034062616728704	23.460125838084842	28.251362228517195	16.25444931666926
4	27.9757916477115	25.254241475988586	28.44510462525453	18.32486225104538
5	22.417724885796634	37.791540157581025	23.415650839283924	16.375084117338417
6	25.609024064462588	34.60183975338177	25.357144414390714	14.431991767764929
7	80.42358802779542	5.6664927393837745	10.04335585667423	3.8665633761465754
8	82.28790439910063	5.74323391378536	8.656927707778909	3.3119339793351132
9	76.92735895538983	7.208002011548966	10.531708784684323	5.33293024837688
10	39.06154845667394	30.06538987568802	17.611954182030647	13.261107485607395
11	28.25107154225052	25.77340714826598	27.70937768430599	18.2661436251775
12	26.31393826114382	23.62581701008827	30.59908986129905	19.461154867468863
13	23.969844206695377	25.220957898454564	31.732766301322478	19.07643159352758
14	20.302400923219583	28.108925957847585	30.845737158570813	20.742935960362022
15	19.856342847010364	26.35957600501143	34.39123755317742	19.392843594800784
16	23.05258369220975	24.839286830313345	31.78465379992355	20.32347567755335
17	22.85825992293907	24.982595159782974	30.5836834891654	21.57546142811256
18	23.94281038389482	24.681298844376748	30.4081089840951	20.96778178763333
19	23.725086370156987	27.065071574226014	28.938689906064734	20.27115214955227
20	24.863849819847186	26.128480423006657	29.508725675009845	19.498944082136312
21	23.719563331090203	26.793570601153732	30.242999184625024	19.243866883131037
22	22.335460672328267	24.562698847574296	30.710132015168007	22.391708464929426
23	21.6660102001811	26.390679435545405	30.400405798028274	21.54290456624522
24	21.091032764702547	25.839974303334024	31.81590257359086	21.253090358372564
25	22.016432494655007	27.16957328709484	29.990828848286476	20.82316536996368
26	21.50715015544448	27.795856848641115	29.602471996011786	21.09452099990262
27	21.61906436811346	27.040363241558836	30.053617081887772	21.286955308439932
28	20.285831806019242	27.151114709161124	31.083809210975733	21.479244273843904
29	21.76164598191641	25.799132882866516	30.847626619304187	21.59159451591289
30	22.298252830194166	26.44561913994654	30.03937345482081	21.21675457503848
31	23.16391653234539	26.824674031687714	27.777398270707398	22.234011165259503
32	23.481491278685283	27.38133823236588	28.089449977980514	21.047720510968322
33	23.863307689959843	26.00188655387071	28.217497278449827	21.917308477719626
34	21.466454078110306	27.858935768509085	28.764423489194463	21.910186664186142
35	22.97962143927491	26.812465208487456	27.94803110924426	22.25988224299337
36	23.697471174823082	27.637578176437845	27.764026702440457	20.90092394629862
37	21.76905848171656	28.93476564146465	28.159069338848617	21.137106537970165
38	21.694788140581693	28.48914359465544	27.646298764438022	22.169769500324843
39	22.161194255457996	27.35517646836534	28.394961244253498	22.088668031923167
40	23.23251849128014	26.30085737914355	28.85380951619631	21.61281461338
41	21.00760580616749	26.528755412214927	28.629981090858355	23.83365769075923
42	21.90568102705272	26.408265954679106	28.74116858786065	22.944884430407527
43	22.229214841859406	26.00188655387071	29.052202893200413	22.71669571106948
44	22.381679788729222	26.567416685682392	28.39161835218676	22.659285173401624
45	22.349704299395228	26.916966921356284	27.05533358429248	23.67799519495601
46	22.320344986461286	27.610689696770617	27.51127499356857	22.557690323199527
47	21.631854563847057	27.17916593389504	29.290565631872006	21.898413870385898
48	21.55365995811211	26.622792419483538	29.104671764334828	22.718875858069524
49	22.017159210321687	25.520074066860747	30.02832737668725	22.43443934613031
50	20.891767328898432	26.221936057741917	29.74941390381482	23.136882709544828
51	21.030424678101294	25.827765480133774	28.28493649231789	24.856873349447042
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	353.0
1	744.0
2	1135.0
3	4728.5
4	8322.0
5	5532.5
6	2743.0
7	2620.5
8	2498.0
9	2509.0
10	2520.0
11	2523.5
12	2527.0
13	2521.0
14	2515.0
15	2373.5
16	2232.0
17	2260.5
18	2289.0
19	2357.5
20	2426.0
21	2887.5
22	3349.0
23	3432.5
24	3516.0
25	4285.0
26	5807.5
27	6561.0
28	8237.5
29	9914.0
30	11849.5
31	13785.0
32	15657.5
33	17530.0
34	19788.0
35	22046.0
36	23659.5
37	25273.0
38	26649.5
39	28026.0
40	31175.5
41	34325.0
42	36990.5
43	39656.0
44	41260.5
45	42865.0
46	45057.0
47	47249.0
48	51260.5
49	55272.0
50	56344.5
51	57417.0
52	55178.0
53	52939.0
54	48627.0
55	44315.0
56	39745.5
57	35176.0
58	32788.0
59	30400.0
60	27271.5
61	24143.0
62	22307.0
63	20471.0
64	17526.5
65	14582.0
66	11550.5
67	8519.0
68	7325.5
69	6132.0
70	4959.5
71	3787.0
72	3109.5
73	2432.0
74	2154.0
75	1353.0
76	830.0
77	703.0
78	576.0
79	418.5
80	261.0
81	179.0
82	97.0
83	84.0
84	71.0
85	41.0
86	11.0
87	6.5
88	2.0
89	4.5
90	7.0
91	4.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	688027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.531960151334953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.51671774269171	23.181262143194946
2	9.13110635950463	5.758433637310024
3	3.6767761542572432	3.47807877544254
4	2.0236314558414996	2.5523626570632842
5	1.4124118083707808	2.22680564294112
6	1.098581977160079	2.078426587607183
7	0.8023166690859993	1.7709032066860066
8	0.658532764825338	1.6611863119056793
9	0.5324969387809908	1.5111605028915045
>10	6.910270472687712	48.613034565165925
>50	0.2003492974906041	3.855233780116284
>100	0.032615001914385704	1.7731342741871123
>500	0.0032615001914385704	0.7116631389644853
>1k	9.318571975538772E-4	0.8283147765238993
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4604	0.669159785880496	No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	1034	0.15028479986977256	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC	926	0.13458774146944816	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCG	894	0.12993676120268535	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGC	810	0.11772793800243304	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.3602940000901126E-4	0.0	0.0	0.08924068386851097	0.0
2	4.3602940000901126E-4	0.0	0.0	0.3890835679413744	0.0
3	4.3602940000901126E-4	0.0	0.0	0.5310838092109758	0.0
4	4.3602940000901126E-4	0.0	0.0	0.7229367452149407	0.0
5	4.3602940000901126E-4	0.0	0.0	1.3140472684938236	0.0
6	4.3602940000901126E-4	0.0	0.0	1.8151903922375139	0.0
7	4.3602940000901126E-4	0.0	0.0	2.252382537313216	0.0
8	4.3602940000901126E-4	0.0	0.0	2.928954822993865	0.0
9	4.3602940000901126E-4	0.0	0.0	3.1912991786659535	0.0
10	4.3602940000901126E-4	0.0	0.0	3.903044502613996	0.0
11	4.3602940000901126E-4	0.0	0.0	4.7081001181639675	0.0
12	4.3602940000901126E-4	0.0	0.0	5.458942744979485	0.0
13	4.3602940000901126E-4	0.0	0.0	5.730879747451771	0.0
14	4.3602940000901126E-4	0.0	0.0	5.843520675787433	0.0
15	4.3602940000901126E-4	0.0	0.0	6.056448366125166	0.0
16	4.3602940000901126E-4	0.0	0.0	6.456723355333439	0.0
17	4.3602940000901126E-4	0.0	0.0	6.961354714277201	0.0
18	4.3602940000901126E-4	0.0	0.0	7.527320875488898	0.0
19	5.813725333453484E-4	0.0	0.0	7.893149542096459	0.0
20	5.813725333453484E-4	0.0	0.0	8.22874683697006	0.0
21	7.267156666816855E-4	0.0	0.0	8.65053260991211	0.0
22	7.267156666816855E-4	0.0	0.0	9.116648038521744	0.0
23	7.267156666816855E-4	0.0	0.0	9.602675476398455	0.0
24	7.267156666816855E-4	0.0	0.0	9.96792277047267	0.0
25	7.267156666816855E-4	0.0	0.0	10.289567124545984	0.0
26	7.267156666816855E-4	0.0	0.0	10.61789726275277	0.0
27	7.267156666816855E-4	0.0	0.0	10.951605096893	0.0
28	7.267156666816855E-4	0.0	0.0	11.285167587899894	0.0
29	8.720588000180225E-4	0.0	0.0	11.634136451040439	0.0
30	8.720588000180225E-4	0.0	0.0	12.056939625915843	0.0
31	8.720588000180225E-4	0.0	0.0	12.442098929257137	0.0
32	8.720588000180225E-4	0.0	0.0	12.812142546731451	0.0
33	8.720588000180225E-4	0.0	0.0	13.178988615272365	0.0
34	8.720588000180225E-4	0.0	0.0	13.52839350781292	0.0
35	8.720588000180225E-4	0.0	0.0	13.895675605753844	0.0
36	8.720588000180225E-4	0.0	0.0	14.27037020349492	0.0
37	8.720588000180225E-4	0.0	0.0	14.643175340502626	0.0
38	8.720588000180225E-4	0.0	0.0	15.02237557537713	0.0
39	8.720588000180225E-4	0.0	0.0	15.442271887585807	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	85	0.0	45.0	2
CGTATCG	20	7.031311E-4	45.0	2
GTCTCGA	20	7.031311E-4	45.0	10
CTCGCTA	20	7.031311E-4	45.0	42
GTATACG	20	7.031311E-4	45.0	1
TACGTCG	20	7.031311E-4	45.0	1
ACGGGTC	80	0.0	42.1875	5
ACTACGG	65	0.0	41.53846	2
CGTGTAC	115	0.0	41.08696	18
CGTTTTT	2395	0.0	41.05428	1
CTCGAGG	45	1.9263098E-8	40.0	2
GCCGTCA	45	1.9263098E-8	40.0	29
CCCTCGT	120	0.0	39.375004	14
CGAATAT	115	0.0	39.130436	14
CTACGAA	110	0.0	38.863636	11
TCGATGG	70	0.0	38.571426	2
TTTGGCG	100	0.0	38.250004	1
TTAGGCG	30	1.1394454E-4	37.500004	1
TCGCAAG	30	1.1394454E-4	37.500004	1
AGATACG	30	1.1394454E-4	37.500004	1
>>END_MODULE