##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551040_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	992595
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.818005329464686	31.0	31.0	34.0	30.0	34.0
2	31.958053385318284	33.0	31.0	34.0	30.0	34.0
3	31.870717664304173	33.0	31.0	34.0	30.0	34.0
4	35.61014008734681	37.0	35.0	37.0	33.0	37.0
5	31.96856220311406	37.0	35.0	37.0	0.0	37.0
6	33.69644416907198	37.0	35.0	37.0	17.0	37.0
7	35.203608722590786	37.0	35.0	37.0	32.0	37.0
8	35.32197724147311	37.0	35.0	37.0	33.0	37.0
9	37.08736795974189	39.0	37.0	39.0	33.0	39.0
10	36.9432084586362	39.0	37.0	39.0	33.0	39.0
11	36.93810869488563	39.0	37.0	39.0	33.0	39.0
12	36.87689541051486	39.0	35.0	39.0	33.0	39.0
13	36.688104413179595	39.0	35.0	39.0	32.0	39.0
14	37.93776515094273	40.0	37.0	41.0	32.0	41.0
15	37.994830721492654	40.0	37.0	41.0	33.0	41.0
16	38.15035739652124	40.0	37.0	41.0	33.0	41.0
17	37.75701670872813	40.0	36.0	41.0	32.0	41.0
18	37.772330104423254	39.0	36.0	41.0	33.0	41.0
19	37.563298223343864	39.0	36.0	41.0	33.0	41.0
20	36.79884343564092	39.0	35.0	41.0	30.0	41.0
21	37.215499775840094	39.0	35.0	41.0	31.0	41.0
22	37.1261984998917	39.0	35.0	41.0	31.0	41.0
23	36.724628876832945	39.0	35.0	41.0	30.0	41.0
24	37.026592920576874	39.0	35.0	41.0	31.0	41.0
25	37.18992237518827	39.0	35.0	41.0	32.0	41.0
26	36.80549871800684	39.0	35.0	41.0	31.0	41.0
27	36.5226945531662	39.0	35.0	41.0	30.0	41.0
28	36.47133221505246	39.0	35.0	41.0	30.0	41.0
29	36.50139986600779	39.0	35.0	41.0	30.0	41.0
30	36.27889924893839	39.0	35.0	41.0	30.0	41.0
31	36.20673184934439	39.0	35.0	41.0	29.0	41.0
32	35.53177378487701	39.0	35.0	41.0	24.0	41.0
33	35.34673859932803	39.0	35.0	41.0	23.0	41.0
34	34.60933915645354	39.0	34.0	41.0	19.0	41.0
35	34.53982036983866	39.0	34.0	41.0	17.0	41.0
36	34.290739929175544	39.0	34.0	41.0	15.0	41.0
37	33.96157848870889	39.0	34.0	41.0	11.0	41.0
38	33.88083357260514	38.0	33.0	41.0	12.0	41.0
39	33.50438094086712	38.0	33.0	41.0	11.0	41.0
40	33.59131569270448	38.0	33.0	41.0	13.0	41.0
41	33.756907903021876	38.0	33.0	40.0	18.0	41.0
42	33.38101441171878	38.0	33.0	40.0	11.0	41.0
43	33.211138480447715	38.0	33.0	40.0	10.0	41.0
44	33.401091079443276	38.0	33.0	40.0	11.0	41.0
45	33.47953394889154	38.0	33.0	40.0	11.0	41.0
46	33.24966778998484	38.0	33.0	40.0	11.0	41.0
47	32.886684901697066	37.0	32.0	40.0	10.0	41.0
48	33.04914592557891	37.0	33.0	40.0	11.0	41.0
49	33.055934192696924	37.0	33.0	40.0	10.0	41.0
50	32.789762189009615	37.0	33.0	40.0	9.0	41.0
51	32.03533062326528	36.0	30.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	10.0
9	37.0
10	26.0
11	22.0
12	18.0
13	22.0
14	22.0
15	36.0
16	74.0
17	225.0
18	605.0
19	1604.0
20	4239.0
21	9215.0
22	15365.0
23	20250.0
24	22419.0
25	22750.0
26	22562.0
27	20623.0
28	17914.0
29	16490.0
30	17423.0
31	20955.0
32	26851.0
33	35587.0
34	53211.0
35	69308.0
36	71616.0
37	103966.0
38	160923.0
39	258022.0
40	200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.605342561669154	14.040973408086883	17.36649892453619	15.987185105707766
2	30.525440889788886	36.86176134274301	18.58764148519789	14.02515628227021
3	24.387892342798423	37.09972345216327	25.67562802552904	12.836756179509266
4	21.558440250051632	19.190707186717646	43.590991290506196	15.659861272724527
5	31.24839436023756	30.498843939371046	22.863101264866334	15.389660435525062
6	19.787022904608627	41.453966622842145	26.480286521693134	12.27872395085609
7	63.33015983356757	4.438466847002051	28.583863509286267	3.6475098101441175
8	62.778575350470234	18.64295105254409	14.40436431777311	4.174109279212569
9	58.505029745263684	5.370166079821075	14.637087633929246	21.487716540986
10	42.90561608712516	21.21580302137327	21.833174658345044	14.045406233156523
11	35.01498597111611	21.11082566404223	27.27708682796105	16.5971015368806
12	22.144782111535925	18.77109999546643	30.896689989371296	28.187427903626354
13	28.904135120567805	20.58130456026879	35.4724736675079	15.04208665165551
14	17.974601927271443	34.27641686689939	29.469521808995612	18.279459396833552
15	15.516701172179992	21.61395130944645	36.7107430523023	26.158604466071257
16	17.856729078828725	22.803661110523425	29.031478095295665	30.308131715352182
17	18.26928404837824	31.119237957072123	31.140898352298773	19.47057964225087
18	19.454863262458506	21.779174789314876	30.240128148942922	28.525833799283696
19	18.815831230260077	23.444103587062195	27.866249578126023	29.873815604551705
20	29.218160478342124	22.571844508586082	32.25414192092445	15.95585309214735
21	19.918395720308887	34.12197321163213	29.495010553146045	16.46462051491293
22	19.86288466091407	19.073741052493716	39.27613981533254	21.78723447125968
23	26.87480795289116	25.35545716027181	30.46559775134874	17.30413713548829
24	20.496476407799758	29.24798130153789	29.599081196258293	20.65646109440406
25	17.860859665825437	36.476911529878755	27.549907061792574	18.112321742503237
26	18.746316473486164	21.707745858079075	38.46150746276175	21.08443020567301
27	29.711110775291026	23.213999667538122	27.327963570237614	19.746925986933242
28	16.888559785209477	31.341483686700013	32.0685677441454	19.701388783945113
29	20.207839048151563	20.421319873664487	39.01289045381047	20.357950624373487
30	20.688901314231884	31.9137211047809	30.67353754552461	16.7238400354626
31	21.121605488643404	23.52328996216987	26.774364166654074	28.580740382532653
32	30.372407678861972	23.369753021121404	26.706159108196193	19.55168019182043
33	30.00891602315144	21.177217294062533	25.809418745812746	23.00444793697329
34	20.819770399810597	22.051692785073467	31.621759126330478	25.506777688785455
35	31.43779688594039	21.644678846861005	26.489958140026896	20.427566127171705
36	20.138828021499204	32.8099577370428	26.387297941254996	20.663916300203002
37	22.12120754184738	22.852321440265165	28.86413894891673	26.162332068970727
38	21.889088701837103	25.554732796357023	24.383056533631542	28.173121968174332
39	21.094001077982462	24.855756879694134	28.05373792936696	25.996504112956444
40	24.522992761398154	21.67067132113299	27.97696945884273	25.829366458626126
41	18.429369481006855	23.970904548179266	27.85869362630277	29.741032344511105
42	24.863111339468766	22.799329031478095	29.368775784685596	22.96878384436754
43	23.156876671754343	23.5266145809721	25.692452611588816	27.624056135684743
44	22.94188465587677	22.00393916955052	30.563220648905144	24.49095552566757
45	21.631481117676394	22.169565633516186	28.284043340939657	27.914909907867763
46	26.10651877150298	25.68681083422745	26.68661437948005	21.520056014789514
47	19.262438356026376	27.908562908336233	32.941028314670135	19.887970420967264
48	21.572746185503654	26.03609730051028	28.80500103264675	23.58615548133932
49	22.424050090923288	21.692331716359643	33.7756083800543	22.108009812662768
50	22.091588210700237	24.035079765664747	30.078531525949657	23.794800497685358
51	20.51773381892917	21.939159475919183	30.439806769125372	27.103299936026275
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	348.0
1	854.5
2	1361.0
3	11711.0
4	22061.0
5	14637.0
6	7213.0
7	7026.0
8	6839.0
9	6505.5
10	6172.0
11	6138.0
12	6104.0
13	5784.0
14	5464.0
15	5267.0
16	5070.0
17	4851.0
18	4632.0
19	4590.5
20	4549.0
21	4800.5
22	5052.0
23	5003.0
24	4954.0
25	5715.5
26	7344.5
27	8212.0
28	9849.0
29	11486.0
30	12913.0
31	14340.0
32	16054.0
33	17768.0
34	20792.0
35	23816.0
36	24986.5
37	26157.0
38	28673.0
39	31189.0
40	33452.0
41	35715.0
42	38496.5
43	41278.0
44	44680.0
45	48082.0
46	54533.0
47	60984.0
48	99987.5
49	138991.0
50	106301.0
51	73611.0
52	73230.0
53	72849.0
54	67753.0
55	62657.0
56	61608.5
57	60560.0
58	56572.5
59	52585.0
60	43909.0
61	35233.0
62	30958.5
63	26684.0
64	22958.0
65	19232.0
66	17036.0
67	14840.0
68	12348.0
69	9856.0
70	8450.0
71	7044.0
72	5858.5
73	4673.0
74	4138.0
75	3115.0
76	2627.0
77	1925.5
78	1224.0
79	902.5
80	581.0
81	415.0
82	249.0
83	184.0
84	119.0
85	73.0
86	27.0
87	23.5
88	20.0
89	12.5
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	992595.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.021592224014388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.64196427265001	22.708922064169638
2	8.280940796237319	4.972140556316835
3	3.415787709544523	3.0764215721923733
4	1.9314461649569892	2.319403566679006
5	1.280635609105694	1.9223360022061715
6	0.9600367203047661	1.7293098562241902
7	0.7472550344895245	1.5703650152950415
8	0.5822099665185746	1.3983096162862172
9	0.4927535477213683	1.3313921468964598
>10	6.452034971786606	41.511429167605286
>50	0.15420749789118177	2.899821747082007
>100	0.05219853621007862	3.055300060456679
>500	0.002388168323336763	0.4578341022541347
>1k	0.004435169743339703	3.3355314035812413
>5k	3.4116690333382333E-4	0.614747266606111
>10k+	0.0013646676133352933	7.096735856148653
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	22776	2.294591449684917	TruSeq Adapter, Index 22 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG	17731	1.7863277570408878	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC	16439	1.6561638936323473	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12342	1.2434074320342132	No Hit
GAACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT	6002	0.604677637908714	No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	4557	0.4590996327807414	TruSeq Adapter, Index 22 (95% over 24bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCCCCGCTCGAATGC	3775	0.38031624177030915	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCT	3666	0.3693349251205174	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT	3197	0.32208503971912006	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTC	2941	0.29629405749575605	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTA	2610	0.2629471234491409	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACGTGCT	2576	0.25952175862260035	No Hit
GAATATGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	2025	0.2040106992277817	TruSeq Adapter, Index 22 (95% over 21bp)
GAATAATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG	1998	0.2012905565714113	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC	1660	0.167238400354626	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1311	0.13207803787043054	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCGCTCGAATGC	1224	0.12331313375545919	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGACCCGCTCGAATGC	1026	0.10336542094207607	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.029840972400627E-4	0.0	0.0	0.06246253507220971	0.0
2	4.029840972400627E-4	0.0	0.0	0.6264387791596774	0.0
3	4.029840972400627E-4	0.0	0.0	0.7631511341483687	0.0
4	4.029840972400627E-4	0.0	0.0	1.5105858885043748	0.0
5	4.029840972400627E-4	0.0	0.0	4.222971101002926	0.0
6	4.029840972400627E-4	0.0	0.0	4.939779063968688	0.0
7	4.029840972400627E-4	0.0	0.0	5.624449045179555	0.0
8	4.029840972400627E-4	0.0	0.0	6.311839169046792	0.0
9	4.029840972400627E-4	0.0	0.0	6.487137251346219	0.0
10	4.029840972400627E-4	0.0	0.0	8.864642679038278	0.0
11	4.029840972400627E-4	0.0	0.0	9.345402707045674	0.0
12	4.029840972400627E-4	0.0	0.0	11.650975473380381	0.0
13	4.029840972400627E-4	0.0	0.0	11.943743420025287	0.0
14	4.029840972400627E-4	0.0	0.0	12.114004201109214	0.0
15	4.029840972400627E-4	0.0	0.0	12.546909867569351	0.0
16	5.037301215500784E-4	0.0	0.0	12.855897924128168	0.0
17	5.037301215500784E-4	0.0	0.0	13.173449392753339	0.0
18	5.037301215500784E-4	0.0	0.0	13.468232259884445	0.0
19	5.037301215500784E-4	0.0	0.0	14.034424916506733	0.0
20	5.037301215500784E-4	0.0	0.0	14.281857152212131	0.0
21	5.037301215500784E-4	0.0	0.0	14.525158800920819	0.0
22	5.037301215500784E-4	0.0	0.0	14.890262393020315	0.0
23	5.037301215500784E-4	0.0	0.0	15.151093849958945	0.0
24	5.037301215500784E-4	0.0	0.0	15.360444088475159	0.0
25	5.037301215500784E-4	0.0	0.0	15.56697343831069	0.0
26	5.037301215500784E-4	0.0	0.0	15.776827406948453	0.0
27	5.037301215500784E-4	0.0	0.0	16.054483449946858	0.0
28	6.04476145860094E-4	0.0	0.0	16.258191911101708	0.0
29	6.04476145860094E-4	0.0	0.0	16.46844886383671	0.0
30	6.04476145860094E-4	0.0	0.0	16.761418302530238	0.0
31	7.052221701701097E-4	0.0	0.0	16.995350570978093	0.0
32	7.052221701701097E-4	0.0	0.0	17.265450662153246	0.0
33	7.052221701701097E-4	0.0	0.0	17.49746875613921	0.0
34	7.052221701701097E-4	0.0	0.0	17.733012960976026	0.0
35	7.052221701701097E-4	0.0	0.0	17.957374357114432	0.0
36	7.052221701701097E-4	0.0	0.0	18.171157420700286	0.0
37	7.052221701701097E-4	0.0	0.0	18.38675391272372	0.0
38	7.052221701701097E-4	0.0	0.0	18.623003339730705	0.0
39	8.059681944801254E-4	0.0	0.0	18.898241478145668	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCGC	20	7.0328196E-4	45.000004	45
ACGTGCG	25	3.8904196E-5	45.0	1
AATAGCG	25	3.8904196E-5	45.0	1
CGATCGA	25	3.8904196E-5	45.0	41
CGTCTAT	30	2.1652013E-6	44.999996	42
GATGAAT	8295	0.0	43.77939	20
CCGATGA	7815	0.0	43.589252	18
CGATGAA	8020	0.0	43.513092	19
TAATACG	1345	0.0	43.494423	4
ATGAATG	8265	0.0	43.366608	21
CGTTATT	810	0.0	43.333336	1
CGTAAGC	270	0.0	43.333332	43
TGAATGA	8235	0.0	43.30601	22
CGCTCGA	1240	0.0	43.185482	41
CGTTTTT	6285	0.0	42.959427	1
CTCGAAT	1315	0.0	42.946766	43
CGGCGAT	510	0.0	42.794117	31
GCGAGCC	830	0.0	42.560238	33
ACCGATG	7975	0.0	42.545452	17
CCGCTCG	1360	0.0	42.518383	40
>>END_MODULE