FastQCFastQC Report
Sat 18 Jun 2016
SRR3551037_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551037_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729219
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103921.4250862909496322No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC103511.419463837338303No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG90641.2429736471485247No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC75641.0372741247828154No Hit
GAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT27890.3824639785853084No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC26050.35723150384178143TruSeq Adapter, Index 14 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT18500.2536960775843745No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTC13530.18554096917386959No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT13040.17882145144325642No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGCGTAT11750.16113129251980543No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTA11620.15934856332596928No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11370.15592023795320747No Hit
GAATATGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC9200.1261623737176349No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG9150.12547670864308252No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC7670.10518102243633257TruSeq Adapter, Index 14 (95% over 22bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC7330.1005184999293765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG302.1644064E-645.0000041
TCGGCAC207.031589E-445.026
CGGGACG453.8380676E-1045.06
GTATACG406.8084773E-945.01
CGTAACG207.031589E-445.043
CCCGGAT253.889398E-545.018
TCGAATA207.031589E-445.044
CGTTATT6650.044.661651
CGTTTTT55350.042.967481
CCGATGA20800.042.6201918
AATACGG4800.042.1875045
CCGATCG750.042.040
CGATGAA21600.041.87500419
TAATACG4900.041.7857134
TACGGCT9600.041.7187547
GATGAAT22550.041.70731720
CCCATCG3700.041.35135340
ATGGGCG2300.041.0869565
ACGGCTG9950.040.251268
ATGAATG23200.040.2478521
Produced by FastQC (version 0.11.5)