##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551037_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	729219
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.969592125273753	33.0	31.0	34.0	30.0	34.0
2	32.114735079585145	33.0	31.0	34.0	30.0	34.0
3	31.983618090038796	33.0	31.0	34.0	30.0	34.0
4	35.697982361951624	37.0	35.0	37.0	33.0	37.0
5	32.144562881658324	37.0	35.0	37.0	0.0	37.0
6	33.850592208924894	37.0	35.0	37.0	17.0	37.0
7	35.40683662932535	37.0	35.0	37.0	32.0	37.0
8	35.56937490657813	37.0	35.0	37.0	33.0	37.0
9	37.47144684930041	39.0	37.0	39.0	35.0	39.0
10	37.17415207228556	39.0	37.0	39.0	34.0	39.0
11	37.139166697521595	39.0	37.0	39.0	34.0	39.0
12	37.12677672962443	39.0	37.0	39.0	34.0	39.0
13	37.04529640615508	39.0	37.0	39.0	33.0	39.0
14	38.338903676399	40.0	38.0	41.0	33.0	41.0
15	38.3874624769788	40.0	38.0	41.0	34.0	41.0
16	38.42726670588671	40.0	38.0	41.0	34.0	41.0
17	38.27711016855019	40.0	37.0	41.0	33.0	41.0
18	38.14172285691953	40.0	37.0	41.0	34.0	41.0
19	37.907521608734825	40.0	37.0	41.0	33.0	41.0
20	37.61933109257987	40.0	35.0	41.0	33.0	41.0
21	37.73633435223164	40.0	35.0	41.0	33.0	41.0
22	37.741504266893756	40.0	35.0	41.0	33.0	41.0
23	37.628918061652264	40.0	35.0	41.0	33.0	41.0
24	37.65175345129515	40.0	35.0	41.0	33.0	41.0
25	37.540051753999826	39.0	35.0	41.0	33.0	41.0
26	37.431871632527404	40.0	35.0	41.0	33.0	41.0
27	37.325025815290054	40.0	35.0	41.0	33.0	41.0
28	37.207958103121285	39.0	35.0	41.0	32.0	41.0
29	37.13443286584688	39.0	35.0	41.0	32.0	41.0
30	36.971669690449644	39.0	35.0	41.0	31.0	41.0
31	36.88225347940742	39.0	35.0	41.0	31.0	41.0
32	36.5191814804606	39.0	35.0	41.0	30.0	41.0
33	36.193213561358114	39.0	35.0	41.0	29.0	41.0
34	35.77150485656572	39.0	35.0	41.0	25.0	41.0
35	35.532122723077705	39.0	35.0	41.0	23.0	41.0
36	35.411039756232356	39.0	35.0	41.0	23.0	41.0
37	35.23629664065253	39.0	35.0	41.0	22.0	41.0
38	35.18727981580293	39.0	35.0	41.0	22.0	41.0
39	34.99338881735117	39.0	35.0	41.0	21.0	41.0
40	34.953851997822326	39.0	35.0	41.0	21.0	41.0
41	34.82647462559259	39.0	34.0	41.0	21.0	41.0
42	34.70221840078221	39.0	35.0	41.0	20.0	41.0
43	34.66588363715153	39.0	35.0	41.0	19.0	41.0
44	34.704859582649384	38.0	35.0	41.0	20.0	41.0
45	34.689138653820045	38.0	34.0	41.0	20.0	41.0
46	34.485151922810566	38.0	34.0	40.0	20.0	41.0
47	34.222354327026586	38.0	34.0	40.0	20.0	41.0
48	34.19785825657313	37.0	34.0	40.0	20.0	41.0
49	34.09762499331477	37.0	34.0	40.0	20.0	41.0
50	33.93794045410227	37.0	34.0	40.0	18.0	41.0
51	32.980998849454004	36.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	28.0
10	26.0
11	25.0
12	16.0
13	16.0
14	18.0
15	23.0
16	66.0
17	169.0
18	338.0
19	808.0
20	1958.0
21	3549.0
22	5557.0
23	7219.0
24	8837.0
25	11211.0
26	13485.0
27	13701.0
28	12741.0
29	12091.0
30	13095.0
31	15124.0
32	18937.0
33	25837.0
34	41053.0
35	53396.0
36	54713.0
37	80374.0
38	128535.0
39	206077.0
40	183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.60690135610839	16.929070690697856	19.96245298051751	18.501574972676245
2	34.8387795710205	29.606332254096507	20.849155054928627	14.705733119954361
3	27.267940083843122	29.311084872994257	29.2422440995092	14.178730943653415
4	24.003077264854593	21.410166218927372	37.814291728547936	16.772464787670096
5	25.896335668708577	32.93880164943591	25.282391160954393	15.88247152090113
6	21.823210859837715	36.13976048347616	28.79752173215454	13.239506924531588
7	69.00848716229281	5.393441476428892	21.427719244835913	4.170352116442386
8	69.37614077526779	11.217754885706489	15.057342170184814	4.348762168840911
9	64.15274423732788	6.577722193195735	16.314166251839296	12.95536731763709
10	38.91492130621939	21.7667120576946	24.18587557373025	15.132491062355754
11	30.371671610311857	22.996795201441543	29.000341461207128	17.63119172703948
12	24.06643271774323	19.939140367982734	33.130102205236014	22.864324709038026
13	24.170379543045367	21.5951586560416	36.63330220413895	17.60115959677408
14	19.710539632126974	28.391882274049358	32.29523641046105	19.60234168336261
15	17.95948816473515	22.97553958413042	38.35610427045922	20.708867980675215
16	20.127972529514455	22.494751233854302	33.10308700129865	24.274189235332596
17	20.05488063256717	25.88262236721753	33.42726944854701	20.635227551668294
18	20.489455156818458	23.188644289301294	33.0631812939597	23.258719259920547
19	20.719564355838234	25.212179057320228	30.733839902690413	23.334416684151126
20	25.062704071067817	24.001431668675664	33.366519522941665	17.569344737314854
21	21.471738942622174	28.45482632789327	31.89892199736979	18.17451273211477
22	20.322290011642593	22.0822551250036	35.04653608860987	22.54891877474394
23	23.11171266793652	25.484799490962253	31.928954127635183	19.474533713466048
24	21.31005911804273	25.245639512958384	31.755892262818165	21.68840910618072
25	19.605770008735373	29.72248391772568	30.299676777483857	20.372069296055095
26	19.748525477257175	23.752398113598247	34.6514558726528	21.847620536491778
27	24.25252221897674	24.83012647777965	30.07957828855255	20.83777301469106
28	19.508131302119118	27.03755661879353	33.0853968423752	20.368915236712155
29	23.504873021684844	23.169034268169096	33.070586476764866	20.255506233381194
30	20.850526385077732	28.916278923067008	31.242329121978447	18.990865569876814
31	24.35372638398067	23.58537010143729	28.60114725480274	23.4597562597793
32	27.56757572142251	24.854673287448627	28.773386321530293	18.80436466959857
33	24.72494545534332	24.431755069464728	27.513682446562694	23.32961702862926
34	25.7552257963657	23.5147465987584	28.473202151891268	22.256825452984632
35	24.06300439237047	25.518808478660045	29.2186572209446	21.199529908024886
36	24.717677405553065	29.039698636486435	27.04523606762852	19.197387890331985
37	23.299310632334045	27.592808196166036	29.10771661188203	20.000164559617893
38	22.767645933526143	25.57763854205664	26.972418436711052	24.68229708770616
39	25.99863689783179	25.75755705761918	27.34199191189478	20.901814132654252
40	21.751353160024628	23.946029930651836	30.733565636660593	23.569051272662943
41	21.038810014549814	27.43469382997426	28.277513339614025	23.2489828158619
42	21.626013584396457	25.14059562353696	31.516320885769574	21.71706990629701
43	22.765863204332305	26.70953444712768	28.16314440517869	22.36145794336132
44	23.056036663882868	24.75429192053416	29.453840341516056	22.735831074066912
45	22.290148775607875	23.37569372163918	29.096060305614635	25.23809719713831
46	25.071754850051903	26.471882932287834	27.511625451339032	20.944736766321228
47	20.217931787295722	26.24835611798376	32.539470310016604	20.99424178470391
48	22.57538544662166	25.266483731224774	29.24443822774777	22.913692594405795
49	21.157292939432462	23.955080709635926	32.92591114603432	21.961715204897295
50	21.1708691079086	24.339875949474713	30.778682398566136	23.710572544050553
51	21.414005943344865	23.604431590509847	29.14364546178857	25.837917004356715
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	371.0
1	800.0
2	1229.0
3	9999.5
4	18770.0
5	12402.0
6	6034.0
7	5831.5
8	5629.0
9	5478.0
10	5327.0
11	5173.5
12	5020.0
13	4877.5
14	4735.0
15	4568.0
16	4401.0
17	4230.0
18	4059.0
19	4059.5
20	4060.0
21	4042.0
22	4024.0
23	4058.0
24	4092.0
25	4556.5
26	5790.5
27	6560.0
28	7896.0
29	9232.0
30	10389.5
31	11547.0
32	12813.0
33	14079.0
34	15923.0
35	17767.0
36	19373.0
37	20979.0
38	22626.5
39	24274.0
40	26312.0
41	28350.0
42	31177.5
43	34005.0
44	35418.5
45	36832.0
46	41139.5
47	45447.0
48	65348.0
49	85249.0
50	69293.5
51	53338.0
52	54207.5
53	55077.0
54	50017.0
55	44957.0
56	42022.0
57	39087.0
58	35565.0
59	32043.0
60	29666.0
61	27289.0
62	24807.5
63	22326.0
64	19066.5
65	15807.0
66	13380.5
67	10954.0
68	9198.0
69	7442.0
70	6214.5
71	4987.0
72	4337.0
73	3687.0
74	2921.0
75	1730.0
76	1305.0
77	1066.5
78	828.0
79	614.5
80	401.0
81	323.0
82	245.0
83	170.0
84	95.0
85	78.5
86	62.0
87	43.5
88	25.0
89	16.5
90	8.0
91	7.5
92	7.0
93	4.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	729219.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.23417808723753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.91369531808634	26.395224820105067
2	8.645479939244453	6.0923275965795725
3	3.6173980020286614	3.823681362476853
4	2.0954642362395184	2.953278403004015
5	1.4405340746019397	2.5378017062629334
6	1.0560132944670027	2.232465628784447
7	0.8787731358726458	2.167399441733189
8	0.7610721645074483	2.145260174519582
9	0.6615638364736948	2.0978692227351226
>10	5.8223938503039685	38.30847926142768
>50	0.07121392175631695	1.657163422637256
>100	0.02927683429276285	1.8733697098354336
>500	0.00237379737508888	0.6417883878326018
>1k	0.003165063166785173	1.8644482378933644
>5k	7.912657916962932E-4	2.317909928948849
>10k+	7.912657916962932E-4	2.891532695224079
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10392	1.4250862909496322	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	10351	1.419463837338303	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG	9064	1.2429736471485247	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	7564	1.0372741247828154	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	2789	0.3824639785853084	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	2605	0.35723150384178143	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	1850	0.2536960775843745	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTC	1353	0.18554096917386959	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	1304	0.17882145144325642	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGCGTAT	1175	0.16113129251980543	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTA	1162	0.15934856332596928	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1137	0.15592023795320747	No Hit
GAATATGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	920	0.1261623737176349	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG	915	0.12547670864308252	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	767	0.10518102243633257	TruSeq Adapter, Index 14 (95% over 22bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	733	0.1005184999293765	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.59931104373309E-4	0.0	0.0	0.06856650745523636	0.0
2	9.59931104373309E-4	0.0	0.0	0.5920032253685107	0.0
3	9.59931104373309E-4	0.0	0.0	0.8077134578226842	0.0
4	9.59931104373309E-4	0.0	0.0	1.3652962964486663	0.0
5	9.59931104373309E-4	0.0	0.0	3.2309909643056476	0.0
6	9.59931104373309E-4	0.0	0.0	3.9634183969424823	0.0
7	9.59931104373309E-4	0.0	0.0	4.678978468745329	0.0
8	9.59931104373309E-4	0.0	0.0	5.466396240361263	0.0
9	9.59931104373309E-4	0.0	0.0	5.791812884743815	0.0
10	9.59931104373309E-4	0.0	0.0	7.730325183518257	0.0
11	9.59931104373309E-4	0.0	0.0	8.45054777782806	0.0
12	9.59931104373309E-4	0.0	0.0	10.199679383011139	0.0
13	0.0010970641192837817	0.0	0.0	10.558007950972204	0.0
14	0.0010970641192837817	0.0	0.0	10.727230091371728	0.0
15	0.0010970641192837817	0.0	0.0	11.077467811453076	0.0
16	0.0010970641192837817	0.0	0.0	11.452663740248129	0.0
17	0.0010970641192837817	0.0	0.0	11.85062374951832	0.0
18	0.0010970641192837817	0.0	0.0	12.252972015265648	0.0
19	0.0010970641192837817	0.0	0.0	12.724983852587494	0.0
20	0.0010970641192837817	0.0	0.0	13.012414651839846	0.0
21	0.0010970641192837817	0.0	0.0	13.307524899927182	0.0
22	0.0010970641192837817	0.0	0.0	13.696982662272925	0.0
23	0.0010970641192837817	0.0	0.0	14.042009327787675	0.0
24	0.0010970641192837817	0.0	0.0	14.323543407398875	0.0
25	0.0010970641192837817	0.0	0.0	14.581764874475295	0.0
26	0.0010970641192837817	0.0	0.0	14.840809139641177	0.0
27	0.0010970641192837817	0.0	0.0	15.130571200147006	0.0
28	0.0010970641192837817	0.0	0.0	15.38125035140335	0.0
29	0.0010970641192837817	0.0	0.0	15.652225188866446	0.0
30	0.0010970641192837817	0.0	0.0	15.971333714563114	0.0
31	0.0012341971341942545	0.0	0.0	16.241348620921837	0.0
32	0.0012341971341942545	0.0	0.0	16.523019833547945	0.0
33	0.0015084631640152	0.0	0.0	16.802908316980222	0.0
34	0.0015084631640152	0.0	0.0	17.074157420473135	0.0
35	0.0015084631640152	0.0	0.0	17.348012051249352	0.0
36	0.0015084631640152	0.0	0.0	17.63503145145697	0.0
37	0.0015084631640152	0.0	0.0	17.92246225070932	0.0
38	0.0015084631640152	0.0	0.0	18.237593918973587	0.0
39	0.0015084631640152	0.0	0.0	18.53229276801619	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	30	2.1644064E-6	45.000004	1
TCGGCAC	20	7.031589E-4	45.0	26
CGGGACG	45	3.8380676E-10	45.0	6
GTATACG	40	6.8084773E-9	45.0	1
CGTAACG	20	7.031589E-4	45.0	43
CCCGGAT	25	3.889398E-5	45.0	18
TCGAATA	20	7.031589E-4	45.0	44
CGTTATT	665	0.0	44.66165	1
CGTTTTT	5535	0.0	42.96748	1
CCGATGA	2080	0.0	42.62019	18
AATACGG	480	0.0	42.187504	5
CCGATCG	75	0.0	42.0	40
CGATGAA	2160	0.0	41.875004	19
TAATACG	490	0.0	41.785713	4
TACGGCT	960	0.0	41.718754	7
GATGAAT	2255	0.0	41.707317	20
CCCATCG	370	0.0	41.351353	40
ATGGGCG	230	0.0	41.086956	5
ACGGCTG	995	0.0	40.25126	8
ATGAATG	2320	0.0	40.24785	21
>>END_MODULE