FastQCFastQC Report
Sat 18 Jun 2016
SRR3551036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508125
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40790.8027552275522756No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC12820.25230012300123No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGC12820.25230012300123No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCG12620.2483640836408364No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC8490.1670848708487085Illumina Single End Adapter 1 (95% over 21bp)
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC7580.14917589175891757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA650.045.00000426
GCGACAC207.029567E-445.035
ATTAGCG207.029567E-445.01
GCGATAT453.8380676E-1045.09
CATCGTT406.8030204E-945.036
CCAGTCG406.8030204E-945.028
CTAGGCG207.029567E-445.01
AGTAACG253.887723E-545.01
TACGTAG207.029567E-445.01
TATAGCG302.1631022E-644.9999961
CGTACAC750.042.029
CACGCGT700.041.78571725
TACGGGA1350.041.6666684
ACGGGTA603.6379788E-1241.2499965
CGTTTTT18850.040.9416431
CGTTATT1650.040.909091
CGCAGTA501.0786607E-940.537
CGCATCG451.9246727E-840.021
CGATGAA2250.040.019
GCATCGT451.9246727E-840.035