##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551031_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 714220 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11663353028479 33.0 31.0 34.0 30.0 34.0 2 32.23201114502534 33.0 31.0 34.0 30.0 34.0 3 32.285129231889336 34.0 31.0 34.0 30.0 34.0 4 35.84546778303604 37.0 35.0 37.0 35.0 37.0 5 35.870733107445886 37.0 35.0 37.0 35.0 37.0 6 35.8907843521604 37.0 35.0 37.0 35.0 37.0 7 36.05410517767635 37.0 35.0 37.0 35.0 37.0 8 35.77851502338215 37.0 35.0 37.0 35.0 37.0 9 37.515407017445604 39.0 37.0 39.0 35.0 39.0 10 37.23163591050377 39.0 37.0 39.0 34.0 39.0 11 37.166731539301615 39.0 37.0 39.0 34.0 39.0 12 36.9994917532413 39.0 37.0 39.0 34.0 39.0 13 36.93164851166307 39.0 37.0 39.0 33.0 39.0 14 37.93281761922097 40.0 37.0 41.0 33.0 41.0 15 38.083460278345605 40.0 37.0 41.0 33.0 41.0 16 38.23968665117191 40.0 37.0 41.0 34.0 41.0 17 38.23327826160007 40.0 37.0 41.0 34.0 41.0 18 38.20073366749741 40.0 37.0 41.0 34.0 41.0 19 38.20972249446949 40.0 37.0 41.0 34.0 41.0 20 38.095474783680096 40.0 37.0 41.0 34.0 41.0 21 38.040183696900115 40.0 36.0 41.0 33.0 41.0 22 38.07129595922825 40.0 36.0 41.0 34.0 41.0 23 38.113458038139505 40.0 36.0 41.0 34.0 41.0 24 38.03836912995995 40.0 36.0 41.0 34.0 41.0 25 37.96629889949875 40.0 36.0 41.0 34.0 41.0 26 37.84362941390608 40.0 36.0 41.0 34.0 41.0 27 37.79821063537845 40.0 36.0 41.0 33.0 41.0 28 37.79451009492873 40.0 36.0 41.0 33.0 41.0 29 37.776948279241694 40.0 36.0 41.0 33.0 41.0 30 37.65688443336787 40.0 35.0 41.0 33.0 41.0 31 37.65967208983226 40.0 35.0 41.0 33.0 41.0 32 37.597066730139176 40.0 35.0 41.0 33.0 41.0 33 37.57095852818459 40.0 35.0 41.0 33.0 41.0 34 37.47263868275881 40.0 35.0 41.0 33.0 41.0 35 37.45104729635126 40.0 35.0 41.0 33.0 41.0 36 37.38950183416874 40.0 35.0 41.0 33.0 41.0 37 37.35637758673798 40.0 35.0 41.0 33.0 41.0 38 37.26890173895998 40.0 35.0 41.0 33.0 41.0 39 37.20199378342807 39.0 35.0 41.0 32.0 41.0 40 37.088338327126095 39.0 35.0 41.0 32.0 41.0 41 36.968387891686035 39.0 35.0 41.0 32.0 41.0 42 36.967633222256445 39.0 35.0 41.0 32.0 41.0 43 36.91468875136513 39.0 35.0 41.0 32.0 41.0 44 36.8359763098205 39.0 35.0 41.0 32.0 41.0 45 36.70201898574669 39.0 35.0 41.0 31.0 41.0 46 36.644624905491305 39.0 35.0 41.0 31.0 41.0 47 36.59401304920053 39.0 35.0 41.0 31.0 41.0 48 36.52893506202571 38.0 35.0 41.0 31.0 41.0 49 36.483001036095324 38.0 35.0 41.0 31.0 41.0 50 36.34654028170592 38.0 35.0 40.0 31.0 41.0 51 35.38359889109798 37.0 34.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 9.0 9 11.0 10 24.0 11 18.0 12 24.0 13 11.0 14 29.0 15 49.0 16 65.0 17 105.0 18 180.0 19 286.0 20 470.0 21 768.0 22 1097.0 23 1688.0 24 2294.0 25 3348.0 26 4393.0 27 5425.0 28 6399.0 29 7947.0 30 9759.0 31 12652.0 32 16978.0 33 24984.0 34 50816.0 35 57273.0 36 50286.0 37 76787.0 38 136987.0 39 242824.0 40 232.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.09730895242362 20.965108790008678 22.922768894738315 11.014813362829381 2 32.71456974041612 23.98378650835877 24.025090308308364 19.27655344291675 3 32.892386099521154 24.15726246814707 25.331130464002687 17.619220968329085 4 28.974545658200558 25.685363053400913 24.992719330178375 20.347371958220155 5 25.78407213463639 29.012909187645263 24.449889389823866 20.753129287894488 6 26.466634930413598 33.746884713393634 24.308616392708128 15.477863963484639 7 79.89821063537845 6.276497437764274 8.515163395032344 5.310128531824928 8 79.93573408753605 5.625857578897259 8.558567388199714 5.879840945366974 9 74.21914816163087 7.831760521968022 10.703704740836157 7.245386575564952 10 42.609000028002576 25.07098653076083 17.433563887877686 14.88644955335891 11 32.88020497885806 24.396824507854724 23.015737447845204 19.70723306544202 12 30.274425247122732 22.114474531656914 26.87939290414718 20.73170731707317 13 27.603679538517547 23.00411637870684 28.64985578673238 20.742348296043236 14 21.81064657948531 25.92856542801938 29.88924981098261 22.371538181512697 15 20.19391784044132 25.397916608327964 33.557867323793786 20.850298227436927 16 25.42843941642631 25.107389879868947 29.463190613536444 20.000980090168298 17 24.93783428075383 25.237181820727507 26.614208507182664 23.210775391336004 18 26.583965724846685 24.111898294643108 28.53700540449721 20.767130576012992 19 25.814034891209992 26.88261320041444 25.749208927221307 21.554142981154268 20 26.968441096580886 27.243426395228358 26.43723222536473 19.350900282826018 21 24.966676934277952 27.26302819859427 28.14524936294139 19.625045504186385 22 23.686399148721684 24.377782756013552 28.92456105961749 23.01125703564728 23 23.547786396348464 25.879280893842232 28.599311136624568 21.973621573184733 24 23.911119823023718 24.677550334630784 28.333146649491752 23.078183192853743 25 24.408025538349527 25.475903783148052 25.718546106241774 24.397524572260647 26 22.909467671025734 25.753969365181597 26.538881577105094 24.797681386687575 27 23.039679650527848 26.189829464310716 27.52457226064798 23.245918624513457 28 19.849346139844865 27.439164403125087 27.050628657836523 25.660860799193525 29 24.02130996051637 27.201002492229286 25.46162246926717 23.316065077987176 30 24.481392288090504 25.740388115706647 25.97981014253311 23.798409453669738 31 24.27851362325334 27.039987678866456 24.650107809918513 24.03139088796169 32 25.84931813726863 27.671305760129933 24.224888689759457 22.254487412841982 33 23.75570552490829 27.812578757245664 24.2591918456498 24.17252387219624 34 21.55638318725323 27.188121307160255 27.775475343731625 23.48002016185489 35 21.50653860155134 29.904091176388224 25.451821567584215 23.137548654476213 36 22.53227296911316 32.33317465206799 22.72101033295063 22.41354204586822 37 21.9373582369578 31.727058889417826 23.914480132172162 22.421102741452216 38 21.131724118618912 31.101201310520565 23.538965584833804 24.228108986026715 39 22.458626193609813 27.579597322953713 24.447929209487274 25.513847273949203 40 24.04105177676346 25.75508946823108 27.085491865251605 23.118366889753858 41 20.186637170619697 28.711881493097362 25.674862087312032 25.426619248970905 42 21.748060821595587 28.23919800621657 26.25871580185377 23.75402537033407 43 22.066730139172805 26.416650331830528 26.544622105233685 24.971997423762986 44 22.291730839237207 25.39147601579345 25.783932121755203 26.53286102321414 45 22.305172075830974 25.092688527344514 25.3629133880317 27.239226008792812 46 22.157038447537172 26.99896390467923 25.171095740808152 25.672901906975444 47 19.973397552574838 27.980454201786564 28.361149225728767 23.68499901990983 48 20.914984178544426 27.20800313628854 26.951219512195124 24.925793172971915 49 23.096384867407803 25.637618660916807 27.52485228641035 23.741144185265046 50 20.222760493965446 26.193049760577974 27.52177200302428 26.062417742432302 51 20.21043936042116 25.179076475035707 25.9114838565148 28.69900030802834 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 369.0 1 706.0 2 1043.0 3 1670.5 4 2298.0 5 1613.5 6 929.0 7 920.0 8 911.0 9 896.5 10 882.0 11 888.5 12 895.0 13 911.0 14 927.0 15 976.5 16 1026.0 17 1110.5 18 1195.0 19 1302.0 20 1409.0 21 1386.0 22 1363.0 23 1713.5 24 2064.0 25 2479.0 26 3709.0 27 4524.0 28 5596.5 29 6669.0 30 7873.0 31 9077.0 32 9843.0 33 10609.0 34 13430.5 35 16252.0 36 17107.0 37 17962.0 38 20178.0 39 22394.0 40 26244.5 41 30095.0 42 34092.5 43 38090.0 44 46354.0 45 54618.0 46 61093.0 47 67568.0 48 70372.5 49 73177.0 50 66861.0 51 60545.0 52 55561.0 53 50577.0 54 48445.0 55 46313.0 56 43021.0 57 39729.0 58 37573.0 59 35417.0 60 33526.5 61 31636.0 62 29902.5 63 28169.0 64 23351.0 65 18533.0 66 15674.0 67 12815.0 68 10538.0 69 8261.0 70 6951.0 71 5641.0 72 4560.0 73 3479.0 74 2787.5 75 1528.5 76 961.0 77 673.5 78 386.0 79 306.5 80 227.0 81 179.0 82 131.0 83 79.5 84 28.0 85 18.5 86 9.0 87 7.5 88 6.0 89 6.0 90 6.0 91 5.0 92 4.0 93 4.0 94 4.0 95 5.5 96 7.0 97 3.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 714220.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.420424494393444 #Duplication Level Percentage of deduplicated Percentage of total 1 70.20902319648204 22.059973121701816 2 11.43289687064909 7.1845294575283365 3 4.380474146078867 4.129090714695409 4 2.157199336618849 2.7112047550235276 5 1.3527452034223695 2.1251914262142733 6 0.966718966351753 1.8224832173732002 7 0.7708450141947533 1.6954194295770126 8 0.6168039960528052 1.5504194708653856 9 0.5502035088382284 1.5558865025401705 >10 7.401354680751367 48.77874853839509 >50 0.10542063748322163 2.153884090334594 >100 0.051358772085913704 2.72901757121576 >500 0.003153608812292947 0.6442117831113089 >1k 0.0018020621784531124 0.8599399214241268 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC 1860 0.26042395900422843 No Hit GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC 1827 0.2558035339251211 No Hit CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT 1216 0.17025566352104393 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1172 0.1640950967489009 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 933 0.1306320181456694 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG 830 0.1162106913836073 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.200386435552071E-4 0.0 0.0 0.17641623029318698 0.0 2 4.200386435552071E-4 0.0 0.0 0.8592590518327686 0.0 3 4.200386435552071E-4 0.0 0.0 1.2326734059533477 0.0 4 4.200386435552071E-4 0.0 0.0 1.7267788636554564 0.0 5 4.200386435552071E-4 0.0 0.0 3.15379014869368 0.0 6 4.200386435552071E-4 0.0 0.0 3.9731455293887037 0.0 7 4.200386435552071E-4 0.0 0.0 4.807622301251715 0.0 8 4.200386435552071E-4 0.0 0.0 6.192769734815603 0.0 9 4.200386435552071E-4 0.0 0.0 6.74400044804122 0.0 10 4.200386435552071E-4 0.0 0.0 7.953571728599031 0.0 11 4.200386435552071E-4 0.0 0.0 9.569040351712358 0.0 12 4.200386435552071E-4 0.0 0.0 10.865559631486096 0.0 13 4.200386435552071E-4 0.0 0.0 11.40782952031587 0.0 14 4.200386435552071E-4 0.0 0.0 11.622889305816136 0.0 15 4.200386435552071E-4 0.0 0.0 12.053288902579038 0.0 16 4.200386435552071E-4 0.0 0.0 12.819579401304921 0.0 17 4.200386435552071E-4 0.0 0.0 13.688639354820644 0.0 18 4.200386435552071E-4 0.0 0.0 14.626585645879421 0.0 19 4.200386435552071E-4 0.0 0.0 15.236621769202767 0.0 20 5.600515247402761E-4 0.0 0.0 15.849878188793369 0.0 21 5.600515247402761E-4 0.0 0.0 16.58634594382683 0.0 22 5.600515247402761E-4 0.0 0.0 17.366497717790036 0.0 23 5.600515247402761E-4 0.0 0.0 18.06628209795301 0.0 24 5.600515247402761E-4 0.0 0.0 18.655316289098597 0.0 25 5.600515247402761E-4 0.0 0.0 19.187505250483046 0.0 26 5.600515247402761E-4 0.0 0.0 19.696872112234324 0.0 27 5.600515247402761E-4 0.0 0.0 20.252583237657866 0.0 28 5.600515247402761E-4 0.0 0.0 20.75494945534989 0.0 29 5.600515247402761E-4 0.0 0.0 21.28601831368486 0.0 30 5.600515247402761E-4 0.0 0.0 21.958500182016746 0.0 31 5.600515247402761E-4 0.0 0.0 22.529472711489458 0.0 32 5.600515247402761E-4 0.0 0.0 23.057321273557168 0.0 33 5.600515247402761E-4 0.0 0.0 23.5952507630702 0.0 34 5.600515247402761E-4 0.0 0.0 24.144241270196858 0.0 35 5.600515247402761E-4 0.0 0.0 24.719834224748677 0.0 36 5.600515247402761E-4 0.0 0.0 25.25160314748957 0.0 37 5.600515247402761E-4 0.0 0.0 25.793172971913418 0.0 38 5.600515247402761E-4 0.0 0.0 26.428971465374815 0.0 39 5.600515247402761E-4 0.0 0.0 27.33849514155302 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTTA 30 2.1643427E-6 45.000004 6 CGATTGA 25 3.889316E-5 45.000004 10 TCTAGCG 25 3.889316E-5 45.000004 1 TCAACGG 25 3.889316E-5 45.000004 2 TATTGCG 20 7.031492E-4 45.0 1 TATCGCG 20 7.031492E-4 45.0 1 TGCGTAT 20 7.031492E-4 45.0 36 CGTAAGG 110 0.0 42.954544 2 TAGGGAC 285 0.0 41.05263 5 CGTTGAT 45 1.9264917E-8 40.0 25 GCGTAAG 45 1.9264917E-8 40.0 1 TTTACGG 40 3.4557706E-7 39.375 2 GCTACGA 115 0.0 39.130432 10 TCAAGCG 190 0.0 39.078945 17 GGCGTAG 35 6.244967E-6 38.571426 1 TACGGTT 35 6.244967E-6 38.571426 33 ATTTGCG 35 6.244967E-6 38.571426 1 TCGTTAG 30 1.139489E-4 37.500004 1 GATCGCC 30 1.139489E-4 37.500004 10 ATACGGA 30 1.139489E-4 37.500004 4 >>END_MODULE