##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551028_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	315208
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94624501916195	33.0	31.0	34.0	30.0	34.0
2	32.05886271922032	33.0	31.0	34.0	30.0	34.0
3	32.132620364965355	33.0	31.0	34.0	30.0	34.0
4	35.73772556534098	37.0	35.0	37.0	33.0	37.0
5	35.75830245425243	37.0	35.0	37.0	35.0	37.0
6	35.761532067714015	37.0	35.0	37.0	35.0	37.0
7	35.93393885941981	37.0	35.0	37.0	35.0	37.0
8	35.46919811679906	37.0	35.0	37.0	33.0	37.0
9	37.30330448465775	39.0	37.0	39.0	34.0	39.0
10	37.05682279637573	39.0	37.0	39.0	33.0	39.0
11	37.035424227811475	39.0	37.0	39.0	33.0	39.0
12	36.92309205350118	39.0	35.0	39.0	33.0	39.0
13	36.842091571279916	39.0	35.0	39.0	33.0	39.0
14	38.00518070607345	40.0	37.0	41.0	33.0	41.0
15	38.104556356437655	40.0	37.0	41.0	33.0	41.0
16	38.290478033552446	40.0	37.0	41.0	34.0	41.0
17	38.20828151569757	40.0	37.0	41.0	34.0	41.0
18	38.20885573970204	40.0	37.0	41.0	34.0	41.0
19	38.209560036547295	40.0	37.0	41.0	34.0	41.0
20	37.97244676531052	40.0	36.0	41.0	33.0	41.0
21	38.036141214689984	40.0	37.0	41.0	34.0	41.0
22	37.992899926397804	40.0	36.0	41.0	33.0	41.0
23	37.9600898454354	40.0	36.0	41.0	34.0	41.0
24	37.88179868531255	40.0	36.0	41.0	33.0	41.0
25	37.843531890053555	40.0	36.0	41.0	33.0	41.0
26	37.672409964214104	40.0	36.0	41.0	33.0	41.0
27	37.59637128499276	40.0	36.0	41.0	33.0	41.0
28	37.608610822060356	40.0	36.0	41.0	33.0	41.0
29	37.60816032588006	40.0	36.0	41.0	33.0	41.0
30	37.52570048983528	40.0	36.0	41.0	33.0	41.0
31	37.57011560620289	40.0	36.0	41.0	33.0	41.0
32	37.412276972665666	40.0	36.0	41.0	33.0	41.0
33	37.377741047181544	40.0	36.0	41.0	33.0	41.0
34	37.33588931753001	40.0	35.0	41.0	32.0	41.0
35	37.28641722291312	40.0	35.0	41.0	32.0	41.0
36	37.22911220527398	40.0	35.0	41.0	32.0	41.0
37	37.18090594147357	40.0	35.0	41.0	32.0	41.0
38	37.11593297124438	40.0	35.0	41.0	32.0	41.0
39	37.025732214918406	39.0	35.0	41.0	32.0	41.0
40	36.987087891170276	39.0	35.0	41.0	31.0	41.0
41	36.85934049897211	39.0	35.0	41.0	31.0	41.0
42	36.88694449379457	39.0	35.0	41.0	31.0	41.0
43	36.83088627192203	39.0	35.0	41.0	31.0	41.0
44	36.806914799116775	39.0	35.0	41.0	31.0	41.0
45	36.68370409380473	39.0	35.0	41.0	31.0	41.0
46	36.63218890383493	39.0	35.0	41.0	31.0	41.0
47	36.48542866932311	39.0	35.0	41.0	31.0	41.0
48	36.46069896703129	39.0	35.0	41.0	31.0	41.0
49	36.46655541737519	39.0	35.0	41.0	31.0	41.0
50	36.331784091774324	39.0	35.0	41.0	31.0	41.0
51	35.360092383442044	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	3.0
11	7.0
12	2.0
13	3.0
14	5.0
15	4.0
16	18.0
17	34.0
18	83.0
19	142.0
20	262.0
21	467.0
22	781.0
23	1283.0
24	1604.0
25	2008.0
26	2357.0
27	2641.0
28	2879.0
29	3700.0
30	4537.0
31	5870.0
32	7818.0
33	10803.0
34	19475.0
35	25774.0
36	22092.0
37	33194.0
38	59603.0
39	107656.0
40	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.82431917971625	19.83166670896678	22.79447222151722	10.549541889799752
2	30.07982030912921	29.7549554579833	23.56602624298876	16.599197989898734
3	30.55855181340575	29.099832491561127	24.282061369000786	16.059554326032334
4	28.22168219080734	23.550163701428897	29.693408796731045	18.534745311032715
5	29.03669957615289	27.483439506611507	23.35124743026827	20.128613486967335
6	24.413720463947616	37.06917337123423	24.040316235628538	14.476789929189614
7	74.26017106164818	6.0115860003553205	14.305791731174336	5.422451206822162
8	72.391880916728	10.458490901246162	10.32714905713053	6.822479124895307
9	66.55002411106317	7.87194487449557	12.09994670186036	13.478084312580899
10	40.66870130199741	24.579008147001343	19.583576558970584	15.168713992030657
11	32.92143600416233	24.50762670998198	22.919786299840105	19.651150986015583
12	27.626836882312634	21.365257226973934	28.675350879419305	22.33255501129413
13	24.343925281084235	24.513971726605924	32.56167356158473	18.58042943072511
14	19.363087231288546	30.765082104515113	27.699487322656786	22.172343341539555
15	17.319991878378723	25.300436537143728	36.63104997335093	20.748521611126623
16	18.91290830181975	25.48602827339408	30.487487627217586	25.113575797568586
17	19.05567117585848	26.891766706428772	28.72515925991726	25.32740285779549
18	19.65622699931474	26.8768559173625	29.81555036674196	23.651366716580796
19	21.528324154209283	27.409202812111367	26.81562650694145	24.2468465267379
20	24.77760716733078	26.907311997157436	29.739727418085838	18.575353417425955
21	22.182495368137864	29.392337757924924	27.590987538387353	20.83417933554986
22	19.284409025151646	24.502550696682825	29.529707367833304	26.683332910332226
23	20.917933554985915	27.68870079439608	29.14995812289028	22.243407527727722
24	22.854749879444682	24.470825613563107	27.91331438288368	24.761110124108527
25	19.215248343950663	31.030621050227154	25.487931778381263	24.266198827440927
26	18.767607421131444	27.37525697317327	28.429481485241492	25.427654120453795
27	22.503553209309406	27.407299307124184	26.54183903961828	23.547308443948122
28	17.188015532600694	27.372084464861295	31.089946955661024	24.349953046876983
29	19.759016268622624	24.16912007309459	28.25245552143347	27.819408136849315
30	18.86024466384102	27.809573361082208	27.334648866780032	25.99553310829674
31	22.58984543539504	25.90828912971752	25.480000507601332	26.02186492728611
32	22.516560493388493	29.6537524428314	23.039389863201443	24.790297200578664
33	21.066089693154996	26.023768432273293	23.888670338316288	29.021471536255426
34	21.859216771147942	25.212240806070913	26.324522220248213	26.60402020253293
35	19.370384000406084	24.615809243420216	24.03524022232938	31.978566533844315
36	19.991878378721353	27.827339407629246	25.305829801274076	26.87495241237532
37	19.63021243115657	26.875904164868913	28.62776325473973	24.866120149234792
38	19.11055556965559	26.99296972158067	25.541864419684778	28.35461028907896
39	23.301439049770313	26.821337021903	25.519656861500977	24.357567066825712
40	21.64126545011548	23.041610619019824	26.985355701631935	28.33176822923276
41	20.217443719702548	26.116088424151673	25.310271312910842	28.35619654323494
42	20.17239410167255	24.474632623537474	27.89808634298622	27.454886931803763
43	21.93472246897287	25.994581355803152	25.568196238674147	26.502499936549835
44	20.417311743356766	24.723040024364863	27.41872033704728	27.440927895231088
45	20.47314788964747	23.356323443567423	26.049465749600266	30.121062917184844
46	23.758597497525443	25.24491764168422	25.670351006319635	25.3261338544707
47	18.818684804954188	23.840448211974316	32.22475317885333	25.116113804218166
48	20.517880256846276	23.98035582853227	27.56180046191721	27.939963452704248
49	20.17429760665973	22.10794142280653	31.877680769523614	25.840080201010128
50	20.561026369889092	22.267835841729905	29.36949569807873	27.801642090302277
51	19.527105911017486	21.556559478185832	26.009174894038225	32.90715971675846
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	139.0
1	385.5
2	632.0
3	841.0
4	1050.0
5	777.0
6	504.0
7	498.0
8	492.0
9	494.5
10	497.0
11	530.0
12	563.0
13	525.0
14	487.0
15	509.0
16	531.0
17	598.0
18	665.0
19	711.5
20	758.0
21	709.5
22	661.0
23	953.0
24	1245.0
25	1394.5
26	2039.0
27	2534.0
28	2742.5
29	2951.0
30	3588.0
31	4225.0
32	4841.0
33	5457.0
34	6218.5
35	6980.0
36	7305.0
37	7630.0
38	8239.5
39	8849.0
40	10503.0
41	12157.0
42	13633.5
43	15110.0
44	17231.0
45	19352.0
46	20632.0
47	21912.0
48	32052.5
49	42193.0
50	38940.0
51	35687.0
52	35231.0
53	34775.0
54	29409.5
55	24044.0
56	20285.5
57	16527.0
58	14746.0
59	12965.0
60	11482.0
61	9999.0
62	8920.0
63	7841.0
64	6479.0
65	5117.0
66	4123.0
67	3129.0
68	2709.0
69	2289.0
70	1933.0
71	1577.0
72	1232.0
73	887.0
74	713.5
75	423.5
76	307.0
77	241.5
78	176.0
79	134.0
80	92.0
81	90.5
82	89.0
83	61.5
84	34.0
85	19.0
86	4.0
87	5.5
88	7.0
89	4.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	315208.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.05534221356438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.64930317239951	22.64687590341126
2	12.113177246707167	7.765840838735196
3	4.458351363892931	4.2874193603369815
4	2.239509722340798	2.871530021609553
5	1.3397025309156319	2.147231154643945
6	0.8968007545173521	1.724835305006188
7	0.7248389558164531	1.6264472544893351
8	0.5668740522065373	1.453707338837641
9	0.48289271113400295	1.3931361997052718
>10	6.363586141545651	42.554910775611155
>50	0.10097756485406287	2.250105649442446
>100	0.051988449231794735	2.912862875342885
>500	0.0069984450888954455	1.5745291348398713
>1k	0.004998889349211032	4.790568187988276
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGC	4373	1.3873378848252582	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	3727	1.1823938478718814	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCG	3621	1.1487652597649807	No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	2195	0.6963655744778052	No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1032	0.3274028577954874	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTACGCAT	887	0.2814014872718967	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	828	0.2626836882312632	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCT	827	0.262366437400066	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTACGCATCGTA	681	0.21604781604527803	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT	598	0.1897159970559123	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTC	583	0.18495723458795463	No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	509	0.16148067307936348	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	478	0.15164589731225095	No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	365	0.11579655338696988	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1725083119717775E-4	0.0	0.0	0.2782289789599249	0.0
2	3.1725083119717775E-4	0.0	0.0	1.6341590314966625	0.0
3	3.1725083119717775E-4	0.0	0.0	2.1839547219613715	0.0
4	3.1725083119717775E-4	0.0	0.0	3.1011268749524126	0.0
5	3.1725083119717775E-4	0.0	0.0	6.209867769853557	0.0
6	3.1725083119717775E-4	0.0	0.0	7.243788228725159	0.0
7	3.1725083119717775E-4	0.0	0.0	8.488680490342885	0.0
8	3.1725083119717775E-4	0.0	0.0	10.221187279510673	0.0
9	3.1725083119717775E-4	0.0	0.0	10.777327986599325	0.0
10	3.1725083119717775E-4	0.0	0.0	13.130377401588792	0.0
11	3.1725083119717775E-4	0.0	0.0	14.977411740818761	0.0
12	3.1725083119717775E-4	0.0	0.0	17.935775741732442	0.0
13	3.1725083119717775E-4	0.0	0.0	18.646100352782923	0.0
14	3.1725083119717775E-4	0.0	0.0	18.944950635770667	0.0
15	3.1725083119717775E-4	0.0	0.0	19.662254765107484	0.0
16	3.1725083119717775E-4	0.0	0.0	20.682850689068804	0.0
17	3.1725083119717775E-4	0.0	0.0	21.81099464480597	0.0
18	3.1725083119717775E-4	0.0	0.0	22.966104921194894	0.0
19	3.1725083119717775E-4	0.0	0.0	23.997804624248115	0.0
20	3.1725083119717775E-4	0.0	0.0	24.764599883251694	0.0
21	3.1725083119717775E-4	0.0	0.0	25.64750894647344	0.0
22	3.1725083119717775E-4	0.0	0.0	26.601482195883353	0.0
23	3.1725083119717775E-4	0.0	0.0	27.495177787365805	0.0
24	3.1725083119717775E-4	0.0	0.0	28.149349001294382	0.0
25	3.1725083119717775E-4	0.0	0.0	28.772112382934445	0.0
26	3.1725083119717775E-4	0.0	0.0	29.31778381259359	0.0
27	3.1725083119717775E-4	0.0	0.0	29.838709677419356	0.0
28	3.1725083119717775E-4	0.0	0.0	30.444976015837163	0.0
29	3.1725083119717775E-4	0.0	0.0	30.992233699652292	0.0
30	3.1725083119717775E-4	0.0	0.0	31.659412197659957	0.0
31	3.1725083119717775E-4	0.0	0.0	32.238712215426006	0.0
32	3.1725083119717775E-4	0.0	0.0	32.808494708256134	0.0
33	3.1725083119717775E-4	0.0	0.0	33.326882566432324	0.0
34	3.1725083119717775E-4	0.0	0.0	33.85383619705084	0.0
35	3.1725083119717775E-4	0.0	0.0	34.43028095733611	0.0
36	3.1725083119717775E-4	0.0	0.0	35.00767747011497	0.0
37	3.1725083119717775E-4	0.0	0.0	35.55620415725489	0.0
38	6.345016623943555E-4	0.0	0.0	36.074274764599885	0.0
39	6.345016623943555E-4	0.0	0.0	36.61835994010304	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCACG	20	7.0254883E-4	45.0	1
GCGAAGT	20	7.0254883E-4	45.0	1
GGCGTAG	20	7.0254883E-4	45.0	1
CGGCACG	20	7.0254883E-4	45.0	15
GCACGTC	20	7.0254883E-4	45.0	17
TACGGGT	20	7.0254883E-4	45.0	4
AAGTTGT	20	7.0254883E-4	45.0	36
CAAGGTA	20	7.0254883E-4	45.0	39
CGGGCAC	35	1.2081728E-7	45.0	6
AGCGACA	20	7.0254883E-4	45.0	28
TCGTAGG	35	1.2081728E-7	45.0	2
TTAGTGT	20	7.0254883E-4	45.0	19
CAGTACG	20	7.0254883E-4	45.0	1
GACTGAT	20	7.0254883E-4	45.0	34
TATCGCG	20	7.0254883E-4	45.0	1
CAATAGT	20	7.0254883E-4	45.0	24
CGATACG	20	7.0254883E-4	45.0	10
AGTAACA	20	7.0254883E-4	45.0	20
TACCGGG	20	7.0254883E-4	45.0	3
ACAACGA	30	2.160472E-6	44.999996	13
>>END_MODULE