FastQCFastQC Report
Sat 18 Jun 2016
SRR3551026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537111
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC21540.40103442305221826Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC18370.3420149652492688Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC18200.3388498839159876No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG17050.3174390396026147No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC16760.31203978321054676No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT12150.22621022470215654Illumina Single End Adapter 2 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG10820.2014481178006036No Hit
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC6920.12883742839003484Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6310.11748037184120229No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6120.11394292799812328No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC5560.10351677772378523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATC453.8380676E-1045.00000426
AGGTACG207.029927E-445.01
CGTATTA207.029927E-445.018
CACGGGT207.029927E-445.04
AACTTAG207.029927E-445.01
GCTATCG207.029927E-445.01
TAAACCG207.029927E-445.01
TAAGTCG253.8880215E-545.024
ATATGCG253.8880215E-545.01
CCGTATT207.029927E-445.017
CCCCGTA207.029927E-445.015
TACGCGG351.2103192E-745.02
CTACGCG207.029927E-445.01
CGGGATC850.045.06
ACTCGAG253.8880215E-545.01
CATCGTT253.8880215E-545.036
AGTTACG253.8880215E-545.01
GGCATAG551.8189894E-1245.01
ACCGTTA207.029927E-445.025
CAATGCG253.8880215E-545.01
Produced by FastQC (version 0.11.5)