##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551023_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	822342
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07344632768361	33.0	31.0	34.0	30.0	34.0
2	32.20196463271972	33.0	31.0	34.0	30.0	34.0
3	32.25276952897943	34.0	31.0	34.0	30.0	34.0
4	35.82657823630558	37.0	35.0	37.0	35.0	37.0
5	35.821014857565345	37.0	35.0	37.0	35.0	37.0
6	35.82854092336279	37.0	35.0	37.0	35.0	37.0
7	35.97596377176406	37.0	35.0	37.0	35.0	37.0
8	35.568368148531874	37.0	35.0	37.0	33.0	37.0
9	37.37778564149709	39.0	37.0	39.0	34.0	39.0
10	37.12207086589278	39.0	37.0	39.0	33.0	39.0
11	37.08061731007294	39.0	37.0	39.0	34.0	39.0
12	36.9480216260388	39.0	37.0	39.0	33.0	39.0
13	36.89396260923071	39.0	37.0	39.0	33.0	39.0
14	37.982943836019565	40.0	37.0	41.0	33.0	41.0
15	38.1045355825192	40.0	37.0	41.0	33.0	41.0
16	38.2950743121475	40.0	37.0	41.0	34.0	41.0
17	38.19928934676813	40.0	37.0	41.0	33.0	41.0
18	38.185780368751686	40.0	37.0	41.0	34.0	41.0
19	38.16756045538231	40.0	37.0	41.0	34.0	41.0
20	37.86710760243305	40.0	36.0	41.0	33.0	41.0
21	37.94224057630524	40.0	36.0	41.0	33.0	41.0
22	37.92595051693821	40.0	36.0	41.0	33.0	41.0
23	37.85911700971129	40.0	36.0	41.0	33.0	41.0
24	37.82114983790199	40.0	36.0	41.0	33.0	41.0
25	37.83265721561102	40.0	36.0	41.0	33.0	41.0
26	37.61446940567306	40.0	35.0	41.0	33.0	41.0
27	37.52101072303251	40.0	35.0	41.0	33.0	41.0
28	37.50058734687028	40.0	35.0	41.0	33.0	41.0
29	37.465387879981805	40.0	35.0	41.0	33.0	41.0
30	37.33701914775118	40.0	35.0	41.0	32.0	41.0
31	37.35238988158211	40.0	35.0	41.0	32.0	41.0
32	37.18333856230133	40.0	35.0	41.0	32.0	41.0
33	37.104384793674654	40.0	35.0	41.0	32.0	41.0
34	36.966178305376594	40.0	35.0	41.0	31.0	41.0
35	36.8632734312488	40.0	35.0	41.0	31.0	41.0
36	36.75988457357158	40.0	35.0	41.0	31.0	41.0
37	36.62205627342395	39.0	35.0	41.0	31.0	41.0
38	36.56938962134976	39.0	35.0	41.0	30.0	41.0
39	36.493101410362115	39.0	35.0	41.0	30.0	41.0
40	36.38595012780571	39.0	35.0	41.0	30.0	41.0
41	36.356901386527746	39.0	35.0	41.0	30.0	41.0
42	36.266132582307606	39.0	35.0	41.0	30.0	41.0
43	36.17280036773021	39.0	35.0	41.0	30.0	41.0
44	36.161213947481706	39.0	35.0	41.0	30.0	41.0
45	36.08029505971968	39.0	35.0	41.0	30.0	41.0
46	36.027475186722796	39.0	35.0	41.0	29.0	41.0
47	35.89407934898132	38.0	35.0	41.0	29.0	41.0
48	35.866315960999195	38.0	35.0	41.0	29.0	41.0
49	35.8555503671222	38.0	35.0	40.0	29.0	41.0
50	35.69197973592495	38.0	35.0	40.0	29.0	41.0
51	34.729797334928776	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	19.0
10	18.0
11	22.0
12	17.0
13	27.0
14	28.0
15	44.0
16	80.0
17	142.0
18	243.0
19	474.0
20	1179.0
21	2080.0
22	3408.0
23	4521.0
24	5529.0
25	6769.0
26	7853.0
27	8654.0
28	9450.0
29	10309.0
30	12328.0
31	15610.0
32	20444.0
33	28780.0
34	53143.0
35	59868.0
36	58532.0
37	85786.0
38	154202.0
39	272523.0
40	250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.6482315143821	19.428899411680298	21.69012406030581	12.232745013631797
2	31.547823168462756	28.89248025760572	22.853022221897945	16.70667435203358
3	30.070092491931582	29.016759450447626	25.356846664769645	15.556301392851148
4	26.98159646473122	23.705587213105982	31.460997978943066	17.85181834321973
5	28.67322841348247	27.499507504177096	24.37234143458561	19.454922647754827
6	24.69009244329002	36.410763405006676	24.56982617937549	14.329317972327814
7	75.33751164357409	5.679632075219312	13.98992146819693	4.992934813009672
8	75.2970175425796	9.514289675098683	9.886276026276173	5.302416756045538
9	69.80842520508499	6.892752650357151	11.029231147138296	12.269590997419565
10	42.405106391258144	23.990991582577564	18.622787112904362	14.981114913259933
11	32.52296489781624	23.490956317444567	24.376475967419882	19.609602817319317
12	27.993097762244908	20.777729946907733	28.424305216077983	22.804867074769376
13	26.946331331733024	21.87544841440666	31.43351549598585	19.744704757874462
14	20.006882781129992	28.29054091849863	29.427902259643794	22.27467404072758
15	18.69818153517636	24.263019522291213	34.57515243049728	22.463646512035137
16	23.11301137482945	25.138446047994627	28.151790860736774	23.596751716439147
17	22.68070948583436	26.962747859163223	28.402538116744612	21.954004538257806
18	23.72017968193282	24.97099746820666	28.04794112425244	23.26088172560808
19	23.09708126302682	26.75431876275321	26.18460445897206	23.963995515247912
20	25.947841652256603	26.892825612701284	28.397673960469	18.76165877457311
21	23.686252191910423	29.358952844436985	28.32848133744841	18.626313626204183
22	22.26324327347989	22.999311721887	30.615607618241558	24.12183738639155
23	23.47697186815218	25.978850648513635	29.722183714318373	20.821993769015812
24	23.182812017384492	25.696729584528093	28.15908709515019	22.96137130293722
25	22.472280389424352	29.73957307300369	25.76738145443137	22.020765083140592
26	21.851614048656153	24.91165476164418	28.548462805012996	24.688268384686662
27	24.155156856879497	25.275372047153134	27.753659669577864	22.815811426389505
28	19.70190018264907	27.865048848289398	28.66544576344149	23.767605205620047
29	23.696345316182317	25.267711001019038	28.266098533213675	22.769845149584967
30	22.644228313767265	26.6740601842056	30.013425071320693	20.668286430706445
31	23.734407339038988	26.164418210428263	25.95854279606295	24.142631654469795
32	26.90340515260074	25.592393432416195	25.338241267015427	22.165960147967635
33	24.549761534738586	24.142996466190468	27.086297428563782	24.22094457050716
34	23.077867845738147	23.957915319903396	27.77129223607696	25.19292459828149
35	24.69422697612429	24.25535847615712	27.644459361190354	23.40595518652823
36	25.55907396192825	28.26318003944831	25.5056898468034	20.672056151820044
37	22.064177677900435	26.223517709176956	27.94944195967128	23.762862653251325
38	22.503897405215834	26.94280481843321	26.276172200860472	24.277125575490487
39	23.442071546874658	24.062981095456635	26.221815254480497	26.273132103188214
40	24.181423300767808	22.40989758518962	29.889267482385673	23.519411631656904
41	22.55570066955111	24.647652679785296	26.33831179728142	26.45833485338217
42	23.87254937726639	23.96618438557194	29.07208922808272	23.08917700907895
43	24.83905722923066	23.80311354643202	27.100403481763063	24.257425742574256
44	22.880626308762047	23.205430344066095	28.506631061042732	25.40731228612913
45	22.4339751587539	22.512774490418828	27.05893654951346	27.994313801313808
46	24.704441704303076	25.14221576910823	26.603651522116103	23.549691004472592
47	20.729818007592947	24.531885760425713	31.918958292292015	22.819337939689326
48	21.617526527892288	24.840881287834016	28.460786388145078	25.08080579612862
49	22.869073937607467	22.50134372317114	31.136801963173472	23.49278037604792
50	21.59490820121069	23.087839366103154	30.241554000646932	25.07569843203922
51	21.21903052501271	22.55120132499617	28.039428850770115	28.190339299221005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	334.0
1	726.0
2	1118.0
3	3865.5
4	6613.0
5	4358.5
6	2104.0
7	2048.5
8	1993.0
9	1931.0
10	1869.0
11	1867.5
12	1866.0
13	1772.5
14	1679.0
15	1696.0
16	1713.0
17	1751.0
18	1789.0
19	1786.5
20	1784.0
21	1945.0
22	2106.0
23	2284.5
24	2463.0
25	3375.5
26	5383.5
27	6479.0
28	7668.0
29	8857.0
30	10755.0
31	12653.0
32	14190.5
33	15728.0
34	18107.5
35	20487.0
36	21847.0
37	23207.0
38	25573.5
39	27940.0
40	29701.0
41	31462.0
42	33607.0
43	35752.0
44	38590.0
45	41428.0
46	45405.0
47	49382.0
48	72603.0
49	95824.0
50	85732.0
51	75640.0
52	75220.0
53	74800.0
54	66368.0
55	57936.0
56	53608.5
57	49281.0
58	46045.0
59	42809.0
60	39097.0
61	35385.0
62	31464.5
63	27544.0
64	23872.0
65	20200.0
66	16746.5
67	13293.0
68	11013.0
69	8733.0
70	7632.0
71	6531.0
72	5131.5
73	3732.0
74	3253.5
75	2123.5
76	1472.0
77	1052.5
78	633.0
79	530.0
80	427.0
81	289.0
82	151.0
83	92.0
84	33.0
85	22.5
86	12.0
87	14.0
88	16.0
89	16.0
90	16.0
91	9.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	822342.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.726668956109876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.70320566827277	22.03200663660879
2	9.599256109155247	5.899063293818572
3	3.69942894480971	3.4101338554145655
4	1.951641844495353	2.398698115068015
5	1.4078242681279876	2.1628875117573174
6	1.0761889282790489	1.9840620560076607
7	0.8918583322203966	1.9182685010919072
8	0.7834315003810732	1.9257792289598163
9	0.698548331265309	1.9317656992168915
>10	8.062903016464086	46.568038694893346
>50	0.07887093092187587	1.5890145729528724
>100	0.039235284696620465	2.305098290290268
>500	0.0024021602875481905	0.5126131164286233
>1k	0.0040036004792469845	2.1347193207069592
>5k	8.007200958493968E-4	1.8881926383640364
>10k+	4.003600479246984E-4	1.3396584684203763
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC	10890	1.3242665460355911	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGC	7907	0.9615220917817647	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCG	7442	0.9049762750777657	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3857	0.46902626887596643	No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	2553	0.31045477429098844	No Hit
GAACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT	2190	0.26631255608980203	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCT	1557	0.1893372830282291	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTACTCGGT	1552	0.18872926349377753	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTC	1276	0.15516658519204907	No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	1222	0.14859997421997176	No Hit
CGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG	1077	0.13096740772087526	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT	1068	0.12987297255886238	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTA	1001	0.12172551079721089	No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	999	0.12148230298343024	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	897	0.10907870448061754	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.864156275612823E-4	0.0	0.0	0.12792731004861724	0.0
2	4.864156275612823E-4	0.0	0.0	0.6847515996993951	0.0
3	4.864156275612823E-4	0.0	0.0	1.0042050631002672	0.0
4	4.864156275612823E-4	0.0	0.0	1.588268627894477	0.0
5	4.864156275612823E-4	0.0	0.0	3.80474303878435	0.0
6	4.864156275612823E-4	0.0	0.0	4.653295101065007	0.0
7	4.864156275612823E-4	0.0	0.0	5.4860386554499225	0.0
8	4.864156275612823E-4	0.0	0.0	6.591661376896717	0.0
9	4.864156275612823E-4	0.0	0.0	7.087318901381663	0.0
10	4.864156275612823E-4	0.0	0.0	8.873558689693583	0.0
11	4.864156275612823E-4	0.0	0.0	9.94233542735261	0.0
12	4.864156275612823E-4	0.0	0.0	11.792782078502619	0.0
13	4.864156275612823E-4	0.0	0.0	12.22958331205265	0.0
14	4.864156275612823E-4	0.0	0.0	12.460144319516697	0.0
15	6.080195344516029E-4	0.0	0.0	12.853654562213775	0.0
16	6.080195344516029E-4	0.0	0.0	13.363782951618669	0.0
17	6.080195344516029E-4	0.0	0.0	13.920120825641886	0.0
18	6.080195344516029E-4	0.0	0.0	14.514642326428664	0.0
19	7.296234413419234E-4	0.0	0.0	15.113663171770382	0.0
20	7.296234413419234E-4	0.0	0.0	15.57162348511933	0.0
21	7.296234413419234E-4	0.0	0.0	16.065578554907813	0.0
22	7.296234413419234E-4	0.0	0.0	16.6637481729013	0.0
23	7.296234413419234E-4	0.0	0.0	17.18749619987791	0.0
24	7.296234413419234E-4	0.0	0.0	17.60484080832549	0.0
25	7.296234413419234E-4	0.0	0.0	17.985947452519756	0.0
26	7.296234413419234E-4	0.0	0.0	18.347232659890896	0.0
27	7.296234413419234E-4	0.0	0.0	18.789870880971662	0.0
28	7.296234413419234E-4	0.0	0.0	19.166478180610987	0.0
29	7.296234413419234E-4	0.0	0.0	19.56156927409764	0.0
30	7.296234413419234E-4	0.0	0.0	20.061361331416855	0.0
31	7.296234413419234E-4	0.0	0.0	20.478705939864437	0.0
32	7.296234413419234E-4	0.0	0.0	20.888632709991708	0.0
33	8.51227348232244E-4	0.0	0.0	21.284818238640373	0.0
34	8.51227348232244E-4	0.0	0.0	21.69036726811959	0.0
35	8.51227348232244E-4	0.0	0.0	22.14832758146854	0.0
36	9.728312551225646E-4	0.0	0.0	22.5445131101172	0.0
37	0.0010944351620128853	0.0	0.0	22.952129406013558	0.0
38	0.0010944351620128853	0.0	0.0	23.432586442137215	0.0
39	0.0010944351620128853	0.0	0.0	24.097151793292817	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	20	7.0321147E-4	45.0	1
TATAGCG	35	1.211356E-7	45.0	1
CGTCGAC	20	7.0321147E-4	45.0	16
CGATCGA	25	3.8898343E-5	45.0	41
CGACGGT	90	0.0	42.5	28
TTGTGCG	80	0.0	42.1875	1
CGATGAA	1450	0.0	41.896553	19
TTTAGCG	65	0.0	41.53846	1
CCGATGA	1445	0.0	41.107265	18
TACGGCT	1000	0.0	40.95	7
ATCCGGC	55	6.002665E-11	40.909092	6
CGTTTTT	1855	0.0	40.754715	1
TCACGAC	95	0.0	40.263157	25
ACCCGTA	95	0.0	40.263157	39
CACCGAT	1535	0.0	39.429966	16
CCGTAAG	40	3.4566256E-7	39.375	42
CGATCAC	40	3.4566256E-7	39.375	34
TGAATGA	1525	0.0	39.2459	22
GATGAAT	1590	0.0	39.198112	20
ATGAATG	1555	0.0	39.067524	21
>>END_MODULE