FastQCFastQC Report
Sat 18 Jun 2016
SRR3551022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311197
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC20840.6696722654781376No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG17670.5678075302782482No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC16400.5269973682265574No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC14250.4579092986114905Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC8080.25964260580918197Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT6850.22011780319219015Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGATGGCT4520.14524561612097803No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCT3940.1266079043178437No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3880.1246798651657953No Hit
TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC3810.12243048615507218Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3270.10507813378663677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGA302.160381E-645.00000441
GTAAGTA302.160381E-645.00000410
GTCGCTA207.025349E-445.020
GGTACAT207.025349E-445.08
GTCGAAG207.025349E-445.01
ATAGCGG453.8198777E-1045.02
AGGCGTC351.2081182E-745.035
CGACAGG207.025349E-445.02
ACGGGTA253.884227E-545.05
CCCGTCT207.025349E-445.040
TCGGAGT207.025349E-445.027
ACCTACG207.025349E-445.035
TACGAAT800.045.012
CGCTAGG207.025349E-445.02
TGCGAAG207.025349E-445.01
TCGCTAT207.025349E-445.021
CAGGCGT351.2081182E-745.034
CGAATAT800.045.014
CTCGCGG207.025349E-445.02
TTAACGG207.025349E-445.02