FastQCFastQC Report
Sat 18 Jun 2016
SRR3551019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353988
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC19480.5503011401516436No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC15300.4322180412895353No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCC14850.41950574595749013No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT10940.30905002429460887No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC8050.22740883871769663No Hit
TGAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATG6040.1706272529012283No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC5270.14887510311083993No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC5210.14718013039990058No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4810.13588031232697154No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4180.11808309886210831No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4090.1155406397956993No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG3610.10198085810818447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG302.1612286E-645.0000042
GACGTAA302.1612286E-645.00000440
TCTACGG253.8853163E-545.0000042
GGTATGA302.1612286E-645.0000048
CGAATAT600.045.00000414
CGGGACG302.1612286E-645.0000046
CATAAGG253.8853163E-545.0000042
GCTACGA650.045.00000410
ATCGCTC253.8853163E-545.00000431
GCACTAG253.8853163E-545.0000041
TCACGCA207.026664E-445.01
GATACGT207.026664E-445.09
GTACCGT207.026664E-445.043
GTACCGG207.026664E-445.02
CTGTACG207.026664E-445.01
GTTTCGA207.026664E-445.034
GGCCGAT406.7921064E-945.08
TTCGTAC207.026664E-445.032
CGATTCG207.026664E-445.010
CGCTAAA207.026664E-445.026