##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551019_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	353988
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02659412183464	33.0	31.0	34.0	30.0	34.0
2	32.112108320056045	33.0	31.0	34.0	30.0	34.0
3	32.18507972021651	34.0	31.0	34.0	30.0	34.0
4	35.77965919748692	37.0	35.0	37.0	33.0	37.0
5	35.820352667322055	37.0	35.0	37.0	35.0	37.0
6	35.83725154525012	37.0	35.0	37.0	35.0	37.0
7	35.96752149790388	37.0	35.0	37.0	35.0	37.0
8	35.64876492988463	37.0	35.0	37.0	33.0	37.0
9	37.350537306349366	39.0	37.0	39.0	34.0	39.0
10	37.163276721244785	39.0	37.0	39.0	34.0	39.0
11	37.15684147485226	39.0	37.0	39.0	34.0	39.0
12	37.05224470886019	39.0	37.0	39.0	34.0	39.0
13	37.01096364848526	39.0	37.0	39.0	33.0	39.0
14	38.15387527260811	40.0	37.0	41.0	33.0	41.0
15	38.334669536820456	40.0	37.0	41.0	34.0	41.0
16	38.40825395211137	40.0	37.0	41.0	34.0	41.0
17	38.418313615150794	40.0	37.0	41.0	34.0	41.0
18	38.36662824728522	40.0	37.0	41.0	34.0	41.0
19	38.388419946438866	40.0	37.0	41.0	34.0	41.0
20	38.2760771551578	40.0	37.0	41.0	34.0	41.0
21	38.2084759935365	40.0	37.0	41.0	34.0	41.0
22	38.20145315660418	40.0	37.0	41.0	34.0	41.0
23	38.231233827135384	40.0	37.0	41.0	34.0	41.0
24	38.139645976699775	40.0	37.0	41.0	34.0	41.0
25	38.04626993005412	40.0	36.0	41.0	34.0	41.0
26	37.90512390250517	40.0	36.0	41.0	34.0	41.0
27	37.859910505440865	40.0	36.0	41.0	33.0	41.0
28	37.916790964665466	40.0	36.0	41.0	34.0	41.0
29	37.907174819485405	40.0	36.0	41.0	34.0	41.0
30	37.78405482671729	40.0	36.0	41.0	33.0	41.0
31	37.83790693469835	40.0	36.0	41.0	33.0	41.0
32	37.78168468987649	40.0	36.0	41.0	33.0	41.0
33	37.757342056792886	40.0	36.0	41.0	33.0	41.0
34	37.713504412578956	40.0	36.0	41.0	33.0	41.0
35	37.73249658180503	40.0	36.0	41.0	33.0	41.0
36	37.663268246381236	40.0	36.0	41.0	33.0	41.0
37	37.647601048623116	40.0	36.0	41.0	33.0	41.0
38	37.57353356610959	40.0	36.0	41.0	33.0	41.0
39	37.51981140603636	40.0	36.0	41.0	33.0	41.0
40	37.43861656327333	40.0	35.0	41.0	33.0	41.0
41	37.349119179181216	40.0	35.0	41.0	33.0	41.0
42	37.358000836186534	40.0	35.0	41.0	33.0	41.0
43	37.309075448885274	40.0	35.0	41.0	33.0	41.0
44	37.24560719572415	39.0	35.0	41.0	33.0	41.0
45	37.139145959750046	39.0	35.0	41.0	32.0	41.0
46	37.09763890301366	39.0	35.0	41.0	32.0	41.0
47	37.09306247669412	39.0	35.0	41.0	32.0	41.0
48	37.04406929048442	39.0	35.0	41.0	32.0	41.0
49	37.046637174141495	39.0	35.0	41.0	32.0	41.0
50	36.9245115653638	39.0	35.0	41.0	32.0	41.0
51	36.02779472750489	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	2.0
9	4.0
10	6.0
11	5.0
12	6.0
13	10.0
14	13.0
15	5.0
16	30.0
17	51.0
18	79.0
19	131.0
20	197.0
21	350.0
22	550.0
23	828.0
24	1112.0
25	1531.0
26	1997.0
27	2463.0
28	2875.0
29	3500.0
30	4515.0
31	5917.0
32	7848.0
33	11023.0
34	20661.0
35	28876.0
36	23835.0
37	35661.0
38	66433.0
39	133354.0
40	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.79495350124863	21.311456885544143	25.09378849000531	10.799801123201917
2	33.64690328485712	23.664926494683435	23.84120365662113	18.846966563838322
3	31.609828581759835	25.269783156491183	25.84663886911421	17.27374939263478
4	29.70806920008588	26.69892764726488	23.91917240132434	19.673830751324903
5	25.381368859961356	29.178390227917326	24.070307468049766	21.369933444071552
6	26.342135891612145	33.38785495553522	24.655073053323843	15.614936099528798
7	75.66979671627287	8.475146050148592	9.78140501937919	6.073652214199352
8	74.0234132230471	6.8101178571025	11.82299964970564	7.343469270144751
9	66.9954913725889	10.19413087449292	13.888323897985241	8.922053854932937
10	36.90125088986067	26.280551883114683	20.608325705956133	16.20987152106851
11	30.30356961252924	24.740386679774456	24.924856209815022	20.031187497881284
12	28.490513802727772	21.743674926833677	30.42193520684317	19.343876063595374
13	24.444896437167362	24.881069414782424	30.350463857531896	20.323570290518322
14	19.100082488671934	29.047312338271354	29.44789088895669	22.404714284100024
15	18.02716476264732	27.072386634575185	34.94186243601478	19.958586166762714
16	19.98175079381222	25.67685910256845	32.22425618947535	22.117133914143984
17	20.183452546414003	26.083652553193893	27.648959851746387	26.083935048645717
18	20.925568098353615	27.53539668011345	30.38012587997333	21.1589093415596
19	22.64850785902347	27.555171361741078	27.156005288314862	22.640315490920596
20	24.212967671220493	27.961964812366517	27.93569273534696	19.889374781066024
21	23.47706701922099	27.817609636484853	29.267376295241647	19.43794704905251
22	20.64222516017492	26.955716012972193	26.66841813846797	25.733640688384916
23	19.70773020554369	27.171542538165134	29.485462784049176	23.635264472241996
24	22.602461100376285	25.107065776241	29.722758963580688	22.567714159802026
25	20.369899544617333	27.049786998429326	27.65686972439744	24.923443732555906
26	19.258844932596585	27.865916358746624	27.982586980349616	24.892651728307175
27	20.659174887284316	27.25403121009752	28.993920697876764	23.092873204741405
28	17.48816344056861	26.546380103280338	30.64623659559081	25.319219860560242
29	19.06505305264585	25.02288213159768	30.203566222583817	25.708498593172653
30	20.533181915771156	26.22942020633468	29.655525046046762	23.58187283184741
31	19.78739392295784	27.320700136727798	27.928912844503202	24.962993095811157
32	20.082601670113114	26.807405900764998	28.344746149586992	24.7652462795349
33	20.293343277173236	27.20063956970293	29.008045470467923	23.497971682655912
34	17.606529034882538	24.774286133993243	30.531260946698758	27.087923884425464
35	18.686509147202727	23.554470773020554	29.05070228369323	28.70831779608348
36	18.768997819135112	26.600336734578573	30.616574573149375	24.014090873136944
37	18.752613082929365	25.784207374261275	32.210978903239656	23.252200639569704
38	17.649750839011492	27.32239510943874	30.381255861780627	24.646598189769144
39	20.307185554312575	25.044634281388067	30.05299614676204	24.595184017537317
40	20.959467552572402	23.751087607489517	29.810050058194065	25.479394781744013
41	17.15199385289897	24.914968869001207	28.590517192673197	29.342520085426628
42	18.710238765155882	23.571985491033594	31.508130219103474	26.209645524707053
43	20.58516107890663	22.57760150061584	32.401098342316686	24.436139078160842
44	19.401505135767312	24.69264494841633	29.47811790230177	26.427732013514582
45	20.271026136479204	23.29542244369866	28.403222708114402	28.030328711707742
46	20.238821654971357	25.07796874470321	28.75973196831531	25.923477632010123
47	17.77546131507283	23.8895103788829	33.095472162898176	25.239556143146096
48	17.891849441223997	22.23860695842797	33.03417064985254	26.835372950495497
49	19.71309761912833	21.34281388069652	33.62910607139225	25.314982428782894
50	18.41757347706702	21.743674926833677	32.295162547882974	27.543589048216322
51	18.315592618958835	21.337163971660054	29.01878029763721	31.328463111743897
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	276.0
1	382.5
2	489.0
3	779.5
4	1070.0
5	773.0
6	476.0
7	456.0
8	436.0
9	454.5
10	473.0
11	492.0
12	511.0
13	530.0
14	549.0
15	626.5
16	704.0
17	685.0
18	666.0
19	717.5
20	769.0
21	848.0
22	927.0
23	1004.0
24	1081.0
25	1565.0
26	2435.0
27	2821.0
28	3551.5
29	4282.0
30	4841.0
31	5400.0
32	6220.5
33	7041.0
34	7745.0
35	8449.0
36	8930.0
37	9411.0
38	10680.0
39	11949.0
40	14642.5
41	17336.0
42	21642.5
43	25949.0
44	30247.0
45	34545.0
46	39738.5
47	44932.0
48	44117.0
49	43302.0
50	36290.5
51	29279.0
52	25241.5
53	21204.0
54	18531.0
55	15858.0
56	15060.5
57	14263.0
58	13110.5
59	11958.0
60	11892.5
61	11827.0
62	10448.0
63	9069.0
64	7685.0
65	6301.0
66	4845.5
67	3390.0
68	2763.5
69	2137.0
70	1660.5
71	1184.0
72	949.5
73	715.0
74	597.0
75	363.5
76	248.0
77	201.5
78	155.0
79	85.5
80	16.0
81	10.0
82	4.0
83	3.5
84	3.0
85	2.5
86	2.0
87	1.0
88	0.0
89	0.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	353988.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.938620816616435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.93529070086605	23.035720220956684
2	12.481106380418984	8.22220860872946
3	4.770144031649852	4.713658964974814
4	2.377189736015729	3.1320540529509846
5	1.4139173385642465	2.32862435405036
6	0.927772560978232	1.8335729154073883
7	0.6770663663785567	1.5611142616879303
8	0.5831685437951571	1.5367014028997645
9	0.47463132169672967	1.4070331019752265
>10	6.160729720184788	42.87548181061165
>50	0.12834853514416433	2.820307375230139
>100	0.06374356778971921	4.117819007717808
>500	0.0034455982589037407	0.697132315989985
>1k	0.0034455982589037407	1.7185716068178023
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1948	0.5503011401516436	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1530	0.4322180412895353	No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCC	1485	0.41950574595749013	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	1094	0.30905002429460887	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	805	0.22740883871769663	No Hit
TGAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATG	604	0.1706272529012283	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	527	0.14887510311083993	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	521	0.14718013039990058	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	481	0.13588031232697154	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	418	0.11808309886210831	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	409	0.1155406397956993	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG	361	0.10198085810818447	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.649909036464513E-4	0.0	0.0	0.3228923014339469	0.0
2	5.649909036464513E-4	0.0	0.0	1.7370645332610144	0.0
3	5.649909036464513E-4	0.0	0.0	2.4246584629987455	0.0
4	5.649909036464513E-4	0.0	0.0	3.302371831813508	0.0
5	5.649909036464513E-4	0.0	0.0	5.771947071652146	0.0
6	5.649909036464513E-4	0.0	0.0	7.037809191272021	0.0
7	5.649909036464513E-4	0.0	0.0	8.276834242968688	0.0
8	5.649909036464513E-4	0.0	0.0	10.215318033379663	0.0
9	5.649909036464513E-4	0.0	0.0	10.911951817575737	0.0
10	5.649909036464513E-4	0.0	0.0	12.386860571544798	0.0
11	5.649909036464513E-4	0.0	0.0	14.919997288043662	0.0
12	5.649909036464513E-4	0.0	0.0	16.618642439856718	0.0
13	5.649909036464513E-4	0.0	0.0	17.559634789879883	0.0
14	5.649909036464513E-4	0.0	0.0	17.84128275534764	0.0
15	5.649909036464513E-4	0.0	0.0	18.46249025390691	0.0
16	5.649909036464513E-4	0.0	0.0	19.68400058759054	0.0
17	5.649909036464513E-4	0.0	0.0	20.983479665977377	0.0
18	5.649909036464513E-4	0.0	0.0	22.382114647954168	0.0
19	5.649909036464513E-4	0.0	0.0	23.131575081641184	0.0
20	5.649909036464513E-4	0.0	0.0	24.068612495338826	0.0
21	5.649909036464513E-4	0.0	0.0	25.062431494852934	0.0
22	5.649909036464513E-4	0.0	0.0	26.061335412499858	0.0
23	5.649909036464513E-4	0.0	0.0	27.03989965761551	0.0
24	5.649909036464513E-4	0.0	0.0	27.768737923319435	0.0
25	5.649909036464513E-4	0.0	0.0	28.46198176209363	0.0
26	5.649909036464513E-4	0.0	0.0	29.069346983513565	0.0
27	8.47486355469677E-4	0.0	0.0	29.663717414149634	0.0
28	8.47486355469677E-4	0.0	0.0	30.272495112828683	0.0
29	8.47486355469677E-4	0.0	0.0	30.919974688407518	0.0
30	8.47486355469677E-4	0.0	0.0	31.6471179814005	0.0
31	8.47486355469677E-4	0.0	0.0	32.33329943387911	0.0
32	0.0011299818072929026	0.0	0.0	33.00705108647751	0.0
33	0.0011299818072929026	0.0	0.0	33.62571612597037	0.0
34	0.0011299818072929026	0.0	0.0	34.24409867001141	0.0
35	0.0011299818072929026	0.0	0.0	34.89016576833113	0.0
36	0.0011299818072929026	0.0	0.0	35.5478151801756	0.0
37	0.0011299818072929026	0.0	0.0	36.15772286066194	0.0
38	0.0011299818072929026	0.0	0.0	36.72751618698939	0.0
39	0.0011299818072929026	0.0	0.0	37.354091099133306	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	30	2.1612286E-6	45.000004	2
GACGTAA	30	2.1612286E-6	45.000004	40
TCTACGG	25	3.8853163E-5	45.000004	2
GGTATGA	30	2.1612286E-6	45.000004	8
CGAATAT	60	0.0	45.000004	14
CGGGACG	30	2.1612286E-6	45.000004	6
CATAAGG	25	3.8853163E-5	45.000004	2
GCTACGA	65	0.0	45.000004	10
ATCGCTC	25	3.8853163E-5	45.000004	31
GCACTAG	25	3.8853163E-5	45.000004	1
TCACGCA	20	7.026664E-4	45.0	1
GATACGT	20	7.026664E-4	45.0	9
GTACCGT	20	7.026664E-4	45.0	43
GTACCGG	20	7.026664E-4	45.0	2
CTGTACG	20	7.026664E-4	45.0	1
GTTTCGA	20	7.026664E-4	45.0	34
GGCCGAT	40	6.7921064E-9	45.0	8
TTCGTAC	20	7.026664E-4	45.0	32
CGATTCG	20	7.026664E-4	45.0	10
CGCTAAA	20	7.026664E-4	45.0	26
>>END_MODULE