FastQCFastQC Report
Sat 18 Jun 2016
SRR3551018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445013
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC17350.38987625080615623No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC11860.26650906827440995No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT11250.2528016035486604No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGC11060.2485320653553941No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCG10510.2361728758485707No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTC9350.21010622161599773No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5370.12067063209389389No Hit
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC4910.11033385541545979No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAGCG302.1624928E-645.0000041
TATCAAC207.0286234E-445.026
GCGAAAG253.8869395E-545.01
TAGCGCG207.0286234E-445.01
GGATCGA253.8869395E-545.08
TTCGTAC351.2096825E-745.033
ATCACCC406.7993824E-945.036
TCTAGCG351.2096825E-745.01
CGTACAT351.2096825E-745.035
TCGTAAG253.8869395E-545.01
CGCACGG800.045.02
ATTATCG253.8869395E-545.030
CCACGTT207.0286234E-445.043
AATCGAT207.0286234E-445.040
CACAACG2100.042.85714312
TAGGGTC850.042.3529435
ACAACGA2200.041.9318213
AAACGGG700.041.7857133
CGTAAGG603.6379788E-1241.2500042
AGCGACT2000.040.519