FastQCFastQC Report
Sat 18 Jun 2016
SRR3551015_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551015_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625384
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC16750.2678354419044939TruSeq Adapter, Index 22 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC12580.20115640950200195TruSeq Adapter, Index 22 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12000.19188210763307023No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT11900.1902830900694613Illumina Single End Adapter 2 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC10080.161180970411779TruSeq Adapter, Index 22 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC8440.13495708236859275No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG7750.12392386117969119No Hit
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG6660.10649456973635399Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG207.030818E-445.040
CGGGTTA253.8887592E-545.06
GATCGGA207.030818E-445.031
TCGATCA453.8380676E-1045.017
CCCCGAA207.030818E-445.024
GCGATAT453.8380676E-1045.09
ATCGGAT207.030818E-445.032
TACAGCG207.030818E-445.01
GGTTCGT253.8887592E-545.09
TCGACGT207.030818E-445.026
CCGCATG207.030818E-445.027
ACGACCG253.8887592E-545.01
CCCGATA207.030818E-445.028
AGTTGCG650.041.538461
CACAACG650.041.5384612
CTCGATC556.002665E-1140.90909216
GTATGCG501.0804797E-940.51
ATGTGCG451.925946E-840.01
GCGTTAG451.925946E-840.01
GGCGATA1250.039.6000028