##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551015_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 625384 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11451524183541 33.0 31.0 34.0 30.0 34.0 2 32.22854758036662 33.0 31.0 34.0 30.0 34.0 3 32.2747511928671 34.0 31.0 34.0 30.0 34.0 4 35.84134387832116 37.0 35.0 37.0 35.0 37.0 5 35.881224975375126 37.0 35.0 37.0 35.0 37.0 6 35.90720581274865 37.0 35.0 37.0 35.0 37.0 7 36.08843846340808 37.0 35.0 37.0 35.0 37.0 8 35.83379491640336 37.0 35.0 37.0 35.0 37.0 9 37.54599094316452 39.0 37.0 39.0 35.0 39.0 10 37.18693474729126 39.0 37.0 39.0 34.0 39.0 11 37.12675252324971 39.0 37.0 39.0 34.0 39.0 12 37.09308520844793 39.0 37.0 39.0 34.0 39.0 13 37.09910391055735 39.0 37.0 39.0 33.0 39.0 14 38.27218796771264 40.0 37.0 41.0 34.0 41.0 15 38.41076522584524 40.0 38.0 41.0 34.0 41.0 16 38.49430589845599 40.0 38.0 41.0 34.0 41.0 17 38.438716052857124 40.0 38.0 41.0 34.0 41.0 18 38.40322106098014 40.0 38.0 41.0 34.0 41.0 19 38.40239756693487 40.0 37.0 41.0 34.0 41.0 20 38.28002475279188 40.0 37.0 41.0 34.0 41.0 21 38.28413262891279 40.0 37.0 41.0 34.0 41.0 22 38.298826001304796 40.0 37.0 41.0 34.0 41.0 23 38.30931555652207 40.0 37.0 41.0 34.0 41.0 24 38.240175636089184 40.0 37.0 41.0 34.0 41.0 25 38.16934235605644 40.0 37.0 41.0 34.0 41.0 26 38.0206385196935 40.0 37.0 41.0 34.0 41.0 27 37.979470213500825 40.0 36.0 41.0 34.0 41.0 28 37.96917413940875 40.0 36.0 41.0 34.0 41.0 29 37.96671804843104 40.0 36.0 41.0 34.0 41.0 30 37.86646124621033 40.0 36.0 41.0 34.0 41.0 31 37.87217133792998 40.0 36.0 41.0 34.0 41.0 32 37.805193289243086 40.0 36.0 41.0 33.0 41.0 33 37.77267406905197 40.0 36.0 41.0 33.0 41.0 34 37.67554014813299 40.0 36.0 41.0 33.0 41.0 35 37.68268615762476 40.0 36.0 41.0 33.0 41.0 36 37.618135417599426 40.0 36.0 41.0 33.0 41.0 37 37.5847335397132 40.0 36.0 41.0 33.0 41.0 38 37.535961905005564 40.0 36.0 41.0 33.0 41.0 39 37.476169841249536 40.0 36.0 41.0 33.0 41.0 40 37.389239251403936 40.0 35.0 41.0 33.0 41.0 41 37.30934913589091 40.0 35.0 41.0 33.0 41.0 42 37.31174766223632 40.0 35.0 41.0 33.0 41.0 43 37.25465474012766 40.0 35.0 41.0 33.0 41.0 44 37.18884557327978 39.0 35.0 41.0 33.0 41.0 45 37.08253329154567 39.0 35.0 41.0 32.0 41.0 46 37.005302342240924 39.0 35.0 41.0 32.0 41.0 47 36.952987284612334 39.0 35.0 41.0 32.0 41.0 48 36.912359446356156 39.0 35.0 41.0 32.0 41.0 49 36.89749018203216 39.0 35.0 41.0 32.0 41.0 50 36.76860936640528 39.0 35.0 41.0 32.0 41.0 51 35.773316874112545 38.0 34.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 5.0 8 11.0 9 19.0 10 10.0 11 24.0 12 12.0 13 11.0 14 18.0 15 21.0 16 35.0 17 63.0 18 108.0 19 190.0 20 354.0 21 559.0 22 951.0 23 1292.0 24 1932.0 25 2815.0 26 3770.0 27 4557.0 28 5103.0 29 6229.0 30 7604.0 31 9825.0 32 13108.0 33 18931.0 34 37750.0 35 47983.0 36 41952.0 37 64473.0 38 123203.0 39 232282.0 40 184.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.75565092806979 21.415002622388805 25.12408376293605 10.70526268660535 2 32.58366059892801 24.688831182121703 25.441168945799703 17.286339273150574 3 32.33645248359408 24.21312345694805 26.2589065278293 17.191517531628566 4 28.745698642753894 25.292780115896797 26.81984188914331 19.141679352206005 5 26.059988742916353 29.556400547503614 24.56106967878935 19.82254103079068 6 25.959890243434437 34.50855794200044 24.612238240824837 14.919313573740295 7 80.87990738490272 5.368701469816944 8.828335870441201 4.923055274839139 8 81.17828406227214 5.087274378621775 8.32064779399537 5.413793765110716 9 74.81947091706856 7.490277973213258 10.577501183272997 7.112749926445192 10 39.94441814948895 27.42634925102017 18.076413851329743 14.552818748161132 11 29.63970936256764 24.687232164558097 25.817897483785963 19.855160989088304 12 28.208108937868577 21.746478963324932 29.223325189003873 20.822086909802618 13 25.33163624269249 23.060071891829658 30.986082151126347 20.622209714351502 14 20.62332902664603 26.867972317807936 30.32009133588323 22.188607319662797 15 20.158654522661276 26.61852557788495 33.49334169086513 19.729478208588645 16 23.787464981515356 25.863149680836095 30.507016489069116 19.842368848579433 17 23.075582362196666 25.949496629270975 28.340507592135395 22.634413416396963 18 23.290810126258428 26.416089954332055 29.441431184680134 20.85166873472938 19 23.794820462307957 28.763927442979032 26.98581991224592 20.455432182467092 20 24.904058946183465 27.6388586852238 28.19739552019239 19.259686848400342 21 23.684808053931665 28.60850293579625 28.602746472567254 19.103942537704835 22 22.011596075371294 25.595474140687962 28.15006460030957 24.242865183631178 23 21.740082893070497 27.169387128548223 28.907039514922033 22.18349046345925 24 23.01481969477953 24.601684724905017 29.80888542079746 22.57461015951799 25 21.752555230066648 27.547874585854448 26.586225423100046 24.113344760978855 26 20.450635129776266 27.78964604147212 29.35508423624525 22.404634592506365 27 22.62657823033528 27.138046384301482 28.332672406073705 21.902702979289522 28 19.234582272651682 28.092180164506924 29.554161922914563 23.119075639926827 29 21.51254269376895 26.194785923528585 29.220287055633023 23.072384327069447 30 24.116702697862433 26.087491845010426 27.976731096414365 21.81907436071278 31 22.72843564913717 28.155980965294923 26.194466120015864 22.921117265552045 32 23.771634707635627 27.133089429854294 26.759718828751616 22.33555703375846 33 25.20947130083277 25.94006242564568 25.866987322988756 22.983478950532792 34 21.400931267829044 25.89816816547913 29.62691722205877 23.073983344633056 35 22.767451677689227 24.610639223261227 28.4492407864608 24.172668312588748 36 23.104364678341625 28.34914228697888 26.613088918168675 21.933404116510815 37 22.666553669425504 27.885906898801377 27.762782546403493 21.68475688536963 38 23.053835723331584 27.710494672073477 24.831943254064704 24.403726350530235 39 23.660183183452087 24.445140905427706 26.831354815601294 25.063321095518916 40 23.851585585816075 23.60149923886764 29.94464201194786 22.602273163368427 41 21.202013482916097 25.06060276566078 28.30484950046691 25.432534250956213 42 22.443650621058424 25.51056630806033 27.4025238893224 24.64325918155885 43 22.186528596830108 24.914772363859644 27.476718304273856 25.421980735036392 44 21.651497320046566 25.06076266741714 26.788980850165657 26.49875916237064 45 22.821178667826487 23.376837271180587 27.50006396070254 26.30192010029038 46 23.928978035894747 25.115129264579846 27.291552070407942 23.66434062911747 47 21.389418341371062 24.749593849538844 30.1096606245123 23.751327184577793 48 21.606884730021875 23.460785693270054 29.768110472925436 25.164219103782635 49 22.586442889488698 22.10593811162422 30.193129341332686 25.1144896575544 50 20.808974965781026 22.039259079221726 30.189451600936383 26.96231435406086 51 20.823366123853503 21.99656531027337 28.3310733885101 28.84899517736303 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 264.0 1 491.5 2 719.0 3 1627.5 4 2536.0 5 1808.0 6 1080.0 7 1071.0 8 1062.0 9 1048.5 10 1035.0 11 1057.5 12 1080.0 13 1089.0 14 1098.0 15 1077.5 16 1057.0 17 1064.0 18 1071.0 19 1267.5 20 1464.0 21 1468.0 22 1472.0 23 2053.5 24 2635.0 25 3095.0 26 4234.0 27 4913.0 28 5814.5 29 6716.0 30 7805.5 31 8895.0 32 10339.5 33 11784.0 34 13745.0 35 15706.0 36 17661.0 37 19616.0 38 20944.0 39 22272.0 40 24361.0 41 26450.0 42 29584.5 43 32719.0 44 35281.5 45 37844.0 46 40824.5 47 43805.0 48 52430.0 49 61055.0 50 65348.0 51 69641.0 52 67583.5 53 65526.0 54 55890.5 55 46255.0 56 40261.5 57 34268.0 58 30657.5 59 27047.0 60 25059.5 61 23072.0 62 20723.5 63 18375.0 64 15070.0 65 11765.0 66 9254.0 67 6743.0 68 5553.5 69 4364.0 70 3504.5 71 2645.0 72 2145.0 73 1645.0 74 1325.0 75 719.0 76 433.0 77 382.5 78 332.0 79 274.5 80 217.0 81 163.5 82 110.0 83 63.5 84 17.0 85 15.5 86 14.0 87 8.5 88 3.0 89 2.0 90 1.0 91 1.5 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 625384.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.97002476931796 #Duplication Level Percentage of deduplicated Percentage of total 1 71.94873541813556 25.8799779511144 2 11.498834490732596 8.272267228998784 3 4.170785692823168 4.50069794035099 4 2.1297673074510577 3.064311312075927 5 1.3424752017250137 2.4144433129121934 6 0.9117744059422476 1.967792877946369 7 0.7508490907655928 1.8905642275000774 8 0.6020812320742774 1.7325501464682587 9 0.5323030712402174 1.7232259191565173 >10 5.979363757594541 42.364789705264826 >50 0.08823897410815161 2.1462079004674854 >100 0.04031222166685 2.480681858650222 >500 0.0022395678703805554 0.5424729374602388 >1k 0.0022395678703805554 1.020016681633731 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC 1675 0.2678354419044939 TruSeq Adapter, Index 22 (95% over 24bp) CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC 1258 0.20115640950200195 TruSeq Adapter, Index 22 (95% over 24bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1200 0.19188210763307023 No Hit CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT 1190 0.1902830900694613 Illumina Single End Adapter 2 (95% over 23bp) GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC 1008 0.161180970411779 TruSeq Adapter, Index 22 (95% over 21bp) GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC 844 0.13495708236859275 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG 775 0.12392386117969119 No Hit CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG 666 0.10649456973635399 Illumina Single End Adapter 2 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.5990175636089186E-4 0.0 0.0 0.2006767042329193 0.0 2 1.5990175636089186E-4 0.0 0.0 0.8741829020249958 0.0 3 1.5990175636089186E-4 0.0 0.0 1.2976027528686376 0.0 4 1.5990175636089186E-4 0.0 0.0 1.9156230412034845 0.0 5 1.5990175636089186E-4 0.0 0.0 3.6208153710360356 0.0 6 1.5990175636089186E-4 0.0 0.0 4.615884000869865 0.0 7 1.5990175636089186E-4 0.0 0.0 5.512293247029025 0.0 8 1.5990175636089186E-4 0.0 0.0 7.0718150768168035 0.0 9 1.5990175636089186E-4 0.0 0.0 7.6313113223235645 0.0 10 1.5990175636089186E-4 0.0 0.0 9.031890806288617 0.0 11 1.5990175636089186E-4 0.0 0.0 10.908337917183683 0.0 12 3.198035127217837E-4 0.0 0.0 12.489926189349264 0.0 13 3.198035127217837E-4 0.0 0.0 13.083161705448173 0.0 14 3.198035127217837E-4 0.0 0.0 13.333407954152968 0.0 15 3.198035127217837E-4 0.0 0.0 13.786409629923375 0.0 16 3.198035127217837E-4 0.0 0.0 14.739104294321569 0.0 17 3.198035127217837E-4 0.0 0.0 15.917740140457703 0.0 18 3.198035127217837E-4 0.0 0.0 17.139549460811278 0.0 19 3.198035127217837E-4 0.0 0.0 17.97583564657874 0.0 20 3.198035127217837E-4 0.0 0.0 18.747681424532768 0.0 21 3.198035127217837E-4 0.0 0.0 19.703414222301816 0.0 22 3.198035127217837E-4 0.0 0.0 20.69176697836849 0.0 23 3.198035127217837E-4 0.0 0.0 21.738483875506887 0.0 24 4.797052690826756E-4 0.0 0.0 22.50984994819183 0.0 25 4.797052690826756E-4 0.0 0.0 23.22013354994691 0.0 26 4.797052690826756E-4 0.0 0.0 23.858941066608676 0.0 27 4.797052690826756E-4 0.0 0.0 24.504144653524875 0.0 28 4.797052690826756E-4 0.0 0.0 25.169495861742547 0.0 29 4.797052690826756E-4 0.0 0.0 25.881698284573957 0.0 30 4.797052690826756E-4 0.0 0.0 26.647947501055352 0.0 31 4.797052690826756E-4 0.0 0.0 27.363667762526703 0.0 32 4.797052690826756E-4 0.0 0.0 28.053643841223952 0.0 33 4.797052690826756E-4 0.0 0.0 28.713558389725353 0.0 34 4.797052690826756E-4 0.0 0.0 29.347888657209012 0.0 35 4.797052690826756E-4 0.0 0.0 30.01292006191396 0.0 36 4.797052690826756E-4 0.0 0.0 30.66739795069909 0.0 37 4.797052690826756E-4 0.0 0.0 31.338505622145753 0.0 38 4.797052690826756E-4 0.0 0.0 32.04591099228634 0.0 39 4.797052690826756E-4 0.0 0.0 32.74628068514705 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTTCG 20 7.030818E-4 45.0 40 CGGGTTA 25 3.8887592E-5 45.0 6 GATCGGA 20 7.030818E-4 45.0 31 TCGATCA 45 3.8380676E-10 45.0 17 CCCCGAA 20 7.030818E-4 45.0 24 GCGATAT 45 3.8380676E-10 45.0 9 ATCGGAT 20 7.030818E-4 45.0 32 TACAGCG 20 7.030818E-4 45.0 1 GGTTCGT 25 3.8887592E-5 45.0 9 TCGACGT 20 7.030818E-4 45.0 26 CCGCATG 20 7.030818E-4 45.0 27 ACGACCG 25 3.8887592E-5 45.0 1 CCCGATA 20 7.030818E-4 45.0 28 AGTTGCG 65 0.0 41.53846 1 CACAACG 65 0.0 41.53846 12 CTCGATC 55 6.002665E-11 40.909092 16 GTATGCG 50 1.0804797E-9 40.5 1 ATGTGCG 45 1.925946E-8 40.0 1 GCGTTAG 45 1.925946E-8 40.0 1 GGCGATA 125 0.0 39.600002 8 >>END_MODULE