FastQCFastQC Report
Sat 18 Jun 2016
SRR3551014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559269
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC10900.19489726768335094No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT7720.13803733087297884No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC7590.13571286804739757No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7060.12623621191233556No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5800.10370680298747113No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5800.10370680298747113No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGG207.030175E-445.0000042
CGCGCAA302.163495E-645.00000418
TTACGTG207.030175E-445.00000414
CACGTCT302.163495E-645.00000440
CTTACGT207.030175E-445.00000413
TCTATCG207.030175E-445.00000410
GGTACTC253.8882285E-545.08
CGTCTAT253.8882285E-545.042
CGTAAGG1150.043.043482
ATGGGCG700.041.7857175
AACGGGC650.041.5384644
TGCAACG403.4539698E-739.3750041
CGCAATC356.242535E-638.5714320
CAATCGG356.242535E-638.5714322
GATAGCG356.242535E-638.571431
ATAACGG659.094947E-1238.0769272
GCGTAAG659.094947E-1238.0769271
CGAATAT950.037.89473714
CCGATTG301.13917245E-437.50000411
ACGGGCC301.13917245E-437.5000045