##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551014_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	559269
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2029488493015	33.0	31.0	34.0	30.0	34.0
2	32.324368059019896	34.0	31.0	34.0	30.0	34.0
3	32.37483035891494	34.0	31.0	34.0	30.0	34.0
4	35.904673779522916	37.0	35.0	37.0	35.0	37.0
5	35.925055742406606	37.0	35.0	37.0	35.0	37.0
6	35.94700939977006	37.0	35.0	37.0	35.0	37.0
7	36.080562305437994	37.0	35.0	37.0	35.0	37.0
8	35.77496875385548	37.0	35.0	37.0	35.0	37.0
9	37.497551267815666	39.0	37.0	39.0	35.0	39.0
10	37.24653073923282	39.0	37.0	39.0	34.0	39.0
11	37.19824985829717	39.0	37.0	39.0	34.0	39.0
12	37.1426504955576	39.0	37.0	39.0	34.0	39.0
13	37.127452084774944	39.0	37.0	39.0	34.0	39.0
14	38.34531504517504	40.0	38.0	41.0	34.0	41.0
15	38.47483947796141	40.0	38.0	41.0	34.0	41.0
16	38.574585753903754	40.0	38.0	41.0	35.0	41.0
17	38.53333905508798	40.0	38.0	41.0	34.0	41.0
18	38.51014628023366	40.0	38.0	41.0	34.0	41.0
19	38.52043113421269	40.0	38.0	41.0	34.0	41.0
20	38.3951711966871	40.0	37.0	41.0	34.0	41.0
21	38.367941008709586	40.0	37.0	41.0	34.0	41.0
22	38.33098026173452	40.0	37.0	41.0	34.0	41.0
23	38.36444895032623	40.0	37.0	41.0	34.0	41.0
24	38.27980274250852	40.0	37.0	41.0	34.0	41.0
25	38.19762761747924	40.0	37.0	41.0	34.0	41.0
26	38.06197554307498	40.0	37.0	41.0	34.0	41.0
27	38.020943410058486	40.0	37.0	41.0	34.0	41.0
28	38.013177916172715	40.0	37.0	41.0	34.0	41.0
29	38.00708603552137	40.0	37.0	41.0	34.0	41.0
30	37.90698751405853	40.0	36.0	41.0	34.0	41.0
31	37.93399061989847	40.0	36.0	41.0	34.0	41.0
32	37.86559061918326	40.0	36.0	41.0	34.0	41.0
33	37.85075697025939	40.0	36.0	41.0	33.0	41.0
34	37.76842092088065	40.0	36.0	41.0	33.0	41.0
35	37.729067765243556	40.0	36.0	41.0	33.0	41.0
36	37.678069050850304	40.0	36.0	41.0	33.0	41.0
37	37.62696305355741	40.0	36.0	41.0	33.0	41.0
38	37.556937716912614	40.0	36.0	41.0	33.0	41.0
39	37.50153682753737	40.0	35.0	41.0	33.0	41.0
40	37.401724393806916	40.0	35.0	41.0	33.0	41.0
41	37.31787923163987	40.0	35.0	41.0	33.0	41.0
42	37.292204645707166	40.0	35.0	41.0	33.0	41.0
43	37.234954914361424	39.0	35.0	41.0	33.0	41.0
44	37.159790726823765	39.0	35.0	41.0	32.0	41.0
45	37.05347516132666	39.0	35.0	41.0	32.0	41.0
46	36.99320541635599	39.0	35.0	41.0	32.0	41.0
47	36.95518793281945	39.0	35.0	41.0	32.0	41.0
48	36.877804777307524	39.0	35.0	41.0	32.0	41.0
49	36.816160023173104	39.0	35.0	41.0	32.0	41.0
50	36.71668016643154	39.0	35.0	41.0	32.0	41.0
51	35.78434349123588	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	13.0
10	9.0
11	20.0
12	13.0
13	13.0
14	20.0
15	24.0
16	38.0
17	61.0
18	100.0
19	186.0
20	312.0
21	491.0
22	777.0
23	1232.0
24	1666.0
25	2267.0
26	3176.0
27	3916.0
28	4580.0
29	5500.0
30	6813.0
31	8684.0
32	11472.0
33	16726.0
34	32905.0
35	42337.0
36	37958.0
37	58444.0
38	108290.0
39	211009.0
40	211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69668263393823	22.212387956421686	23.790698214991355	10.30023119464873
2	31.893418015302117	24.56295628758254	25.200931930788222	18.342693766327116
3	33.45527822925998	24.18281721318364	24.678821819196127	17.683082738360252
4	29.75401819160368	25.931707282184423	25.00335259061382	19.310921935598074
5	26.94338502581048	29.33686651682822	23.19724497513719	20.522503482224117
6	27.475150598370373	34.99317859563108	22.476303889541526	15.05536691645702
7	80.34291190822306	6.6413479023511055	7.742785672011143	5.272954517414696
8	80.82246646962375	6.3751075064056835	7.548782428491477	5.253643595479099
9	74.12854994644795	8.809892913785673	9.841596798678275	7.219960341088099
10	41.08935056296701	27.149368193123525	16.933175269861195	14.828105974048267
11	31.003327557937236	26.606516720933932	23.39929443613002	18.99086128499881
12	30.8495554017834	22.745762772476212	26.532312715348073	19.872369110392317
13	26.34331600714504	23.854889149943943	27.666829379064456	22.134965463846555
14	20.27879249520356	28.000658001784473	28.148887208123462	23.571662294888508
15	20.218714071403923	27.212307494246957	32.202392766271686	20.366585668077438
16	23.133054040184597	26.083870194843627	30.00541778643193	20.777657978539843
17	22.356862261273196	26.53123988635165	27.778045985026882	23.333851867348272
18	23.630310280026247	27.299743057455355	27.894805540804157	21.17514112171424
19	25.441066821154042	28.58999873048569	25.33306870218088	20.635865746179388
20	27.554003529607396	26.755818756269345	26.125710525704086	19.56446718841917
21	25.70820124126315	26.79068569865306	26.721488228383837	20.779624831699948
22	23.7928438729842	25.671188640886587	27.189956890154825	23.346010595974388
23	24.05372012394751	25.88414519667638	28.15443015793831	21.907704521437804
24	24.03977334699402	25.1365621910029	27.720649633718303	23.103014828284778
25	23.026665164706074	27.22696949053139	26.052400544281912	23.69396480048063
26	22.569997621895723	28.600548215617174	25.759339423425935	23.070114739061168
27	23.308461581099614	27.70169632144818	26.831453200517103	22.158388896935104
28	20.47440498221786	29.004110723104624	27.287941938494715	23.233542356182806
29	22.44376140998339	28.50953655575403	26.30558818743753	22.74111384682505
30	23.510511042092446	27.251823362281836	26.38158024135076	22.856085354274956
31	23.50782896960139	28.309453947921305	24.315132789409034	23.867584293068273
32	23.988456359998498	29.57789543135772	24.48750064816752	21.946147560476266
33	24.813998272745316	28.58195251301252	24.357509534767704	22.246539679474456
34	22.641340750157795	27.83293906867715	26.219583062891022	23.306137118274034
35	23.64980000679458	27.489991399487547	25.421219484720233	23.43898910899764
36	23.701474603455583	29.946412191628713	24.35178778012012	22.00032542479558
37	24.308338205765025	28.860172832751324	25.012471637083404	21.819017324400246
38	22.63150648435726	28.557456250927547	25.021054269054783	23.78998299566041
39	24.442799439983265	26.36137529525148	26.00716292159945	23.18866234316581
40	24.20266454961745	26.25766849226401	27.55972528425498	21.97994167386356
41	21.78254113852189	26.992913964478632	27.0574625090967	24.16708238790278
42	22.963368253917167	27.212307494246957	25.528502384362444	24.295821867473432
43	24.076070728039635	26.594000382642342	25.77114054238658	23.558788346931443
44	22.29803547130272	27.36250355374605	25.609500973592315	24.729960001358915
45	23.081200638690863	26.359229637258636	25.47235766688302	25.08721205716748
46	22.887197395171196	28.14101979548303	25.00460422444298	23.967178584902793
47	23.022910263218595	26.711832767416038	27.40112539761725	22.86413157174812
48	22.809417292930593	24.796654203969826	27.876567447864982	24.517361055234602
49	22.886124566174775	25.74110133048676	27.372337819546587	24.000436283791878
50	20.837021183008535	25.707664826764937	28.1345828215045	25.320731168722027
51	21.23325269235377	24.68293433034908	26.29485989747331	27.78895307982384
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	165.0
1	533.0
2	901.0
3	1261.0
4	1621.0
5	1234.5
6	848.0
7	822.5
8	797.0
9	796.0
10	795.0
11	772.5
12	750.0
13	802.0
14	854.0
15	843.5
16	833.0
17	893.0
18	953.0
19	974.0
20	995.0
21	1209.5
22	1424.0
23	1618.5
24	1813.0
25	2603.5
26	4286.0
27	5178.0
28	6031.0
29	6884.0
30	8491.0
31	10098.0
32	11334.0
33	12570.0
34	14098.0
35	15626.0
36	16483.0
37	17340.0
38	18662.0
39	19984.0
40	22161.5
41	24339.0
42	26392.0
43	28445.0
44	30731.0
45	33017.0
46	35184.5
47	37352.0
48	40616.5
49	43881.0
50	47316.5
51	50752.0
52	49851.0
53	48950.0
54	43513.0
55	38076.0
56	35187.0
57	32298.0
58	30644.5
59	28991.0
60	28053.0
61	27115.0
62	24374.5
63	21634.0
64	18663.0
65	15692.0
66	12879.0
67	10066.0
68	8190.5
69	6315.0
70	4955.5
71	3596.0
72	2893.0
73	2190.0
74	1760.5
75	1008.0
76	685.0
77	578.0
78	471.0
79	310.5
80	150.0
81	111.5
82	73.0
83	42.0
84	11.0
85	8.0
86	5.0
87	4.5
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	559269.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.281066783621657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.88482171712211	20.755831986441674
2	10.15511475241844	5.947051865218117
3	3.5560410143007295	3.1237402327511203
4	1.8511781680260977	2.168178862654182
5	1.1678200685437485	1.7097508709141567
6	0.9649771337648133	1.6953335939061187
7	0.7834703714776445	1.6058593789158044
8	0.7222801279497724	1.6919306130384064
9	0.6633998360336227	1.7482549412829749
>10	9.077933134849413	53.612252416407436
>50	0.11736820838425968	2.342721186260693
>100	0.05127137524154502	2.6872922069390923
>500	0.0037063644752924115	0.7146457232022854
>1k	6.177274125487352E-4	0.19715612206795521
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	1090	0.19489726768335094	No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	772	0.13803733087297884	No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	759	0.13571286804739757	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	706	0.12623621191233556	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	580	0.10370680298747113	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	580	0.10370680298747113	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.364144982110576E-4	0.0	0.0	0.14447430485151153	0.0
2	5.364144982110576E-4	0.0	0.0	0.6340419368854702	0.0
3	5.364144982110576E-4	0.0	0.0	0.8866931655428782	0.0
4	5.364144982110576E-4	0.0	0.0	1.2809578217280058	0.0
5	5.364144982110576E-4	0.0	0.0	2.4051038051456453	0.0
6	5.364144982110576E-4	0.0	0.0	3.016258723440777	0.0
7	5.364144982110576E-4	0.0	0.0	3.6218706919210613	0.0
8	5.364144982110576E-4	0.0	0.0	4.52715956006859	0.0
9	5.364144982110576E-4	0.0	0.0	4.851869136319016	0.0
10	5.364144982110576E-4	0.0	0.0	5.6568484933010765	0.0
11	7.152193309480769E-4	0.0	0.0	6.856450116133739	0.0
12	7.152193309480769E-4	0.0	0.0	7.78677166086445	0.0
13	7.152193309480769E-4	0.0	0.0	8.16047376128482	0.0
14	8.940241636850961E-4	0.0	0.0	8.318358428591608	0.0
15	8.940241636850961E-4	0.0	0.0	8.666670242763322	0.0
16	8.940241636850961E-4	0.0	0.0	9.300354569983318	0.0
17	8.940241636850961E-4	0.0	0.0	10.005203220632648	0.0
18	8.940241636850961E-4	0.0	0.0	10.775315635230989	0.0
19	8.940241636850961E-4	0.0	0.0	11.223042936404486	0.0
20	8.940241636850961E-4	0.0	0.0	11.70617359445991	0.0
21	8.940241636850961E-4	0.0	0.0	12.308745880783666	0.0
22	8.940241636850961E-4	0.0	0.0	12.99106512250813	0.0
23	8.940241636850961E-4	0.0	0.0	13.618133670916858	0.0
24	8.940241636850961E-4	0.0	0.0	14.107343693285342	0.0
25	8.940241636850961E-4	0.0	0.0	14.561865578102845	0.0
26	8.940241636850961E-4	0.0	0.0	14.941110628338063	0.0
27	8.940241636850961E-4	0.0	0.0	15.335196479690453	0.0
28	8.940241636850961E-4	0.0	0.0	15.747878033647494	0.0
29	8.940241636850961E-4	0.0	0.0	16.16789058574675	0.0
30	8.940241636850961E-4	0.0	0.0	16.67498109138894	0.0
31	8.940241636850961E-4	0.0	0.0	17.10858281077621	0.0
32	8.940241636850961E-4	0.0	0.0	17.565250353586556	0.0
33	0.0010728289964221152	0.0	0.0	17.990090636169715	0.0
34	0.0010728289964221152	0.0	0.0	18.435851084183103	0.0
35	0.0010728289964221152	0.0	0.0	18.882684361192915	0.0
36	0.0010728289964221152	0.0	0.0	19.358305216273386	0.0
37	0.0010728289964221152	0.0	0.0	19.83499890035028	0.0
38	0.0010728289964221152	0.0	0.0	20.32975187253361	0.0
39	0.0010728289964221152	0.0	0.0	20.811273287094405	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACCGG	20	7.030175E-4	45.000004	2
CGCGCAA	30	2.163495E-6	45.000004	18
TTACGTG	20	7.030175E-4	45.000004	14
CACGTCT	30	2.163495E-6	45.000004	40
CTTACGT	20	7.030175E-4	45.000004	13
TCTATCG	20	7.030175E-4	45.000004	10
GGTACTC	25	3.8882285E-5	45.0	8
CGTCTAT	25	3.8882285E-5	45.0	42
CGTAAGG	115	0.0	43.04348	2
ATGGGCG	70	0.0	41.785717	5
AACGGGC	65	0.0	41.538464	4
TGCAACG	40	3.4539698E-7	39.375004	1
CGCAATC	35	6.242535E-6	38.57143	20
CAATCGG	35	6.242535E-6	38.57143	22
GATAGCG	35	6.242535E-6	38.57143	1
ATAACGG	65	9.094947E-12	38.076927	2
GCGTAAG	65	9.094947E-12	38.076927	1
CGAATAT	95	0.0	37.894737	14
CCGATTG	30	1.13917245E-4	37.500004	11
ACGGGCC	30	1.13917245E-4	37.500004	5
>>END_MODULE