FastQCFastQC Report
Sat 18 Jun 2016
SRR3551013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444234
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC12510.2816083415497238Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC11040.24851767311822145Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT8270.1861631482506967Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6520.1467694953560511No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGA600.045.00000410
GCGTAAG302.1624837E-645.0000041
TGATCGC207.028608E-445.037
TCACGAC502.1827873E-1145.025
GTTTACG207.028608E-445.010
CACGATC207.028608E-445.08
GCGTTAG253.8869286E-545.01
CGGATAT207.028608E-445.010
ACGGATA207.028608E-445.09
CGATCAT207.028608E-445.010
CGATATT207.028608E-445.033
CTAGGGT502.1827873E-1145.04
TAGTCCG253.8869286E-545.01
TGCACCG207.028608E-445.01
AATCGTT253.8869286E-545.022
CTTACGA207.028608E-445.029
CGGGGTA1100.040.909096
CTAGCGG556.002665E-1140.909092
CGGTCTA501.0786607E-940.531
CTCACGT451.9237632E-840.045