##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551013_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	444234
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19301989492025	33.0	31.0	34.0	30.0	34.0
2	32.310361206031054	34.0	31.0	34.0	30.0	34.0
3	32.35239085707082	34.0	31.0	34.0	30.0	34.0
4	35.88916877141327	37.0	35.0	37.0	35.0	37.0
5	35.92340748344341	37.0	35.0	37.0	35.0	37.0
6	35.943174092933006	37.0	35.0	37.0	35.0	37.0
7	36.08624508704872	37.0	35.0	37.0	35.0	37.0
8	35.80889576214337	37.0	35.0	37.0	35.0	37.0
9	37.55576340397178	39.0	38.0	39.0	35.0	39.0
10	37.277099456592694	39.0	37.0	39.0	34.0	39.0
11	37.18833092469284	39.0	37.0	39.0	34.0	39.0
12	37.082006329997256	39.0	37.0	39.0	34.0	39.0
13	37.06305460635611	39.0	37.0	39.0	33.0	39.0
14	38.243574332446414	40.0	37.0	41.0	34.0	41.0
15	38.3859722578641	40.0	38.0	41.0	34.0	41.0
16	38.46596838603079	40.0	38.0	41.0	34.0	41.0
17	38.42455102491029	40.0	37.0	41.0	34.0	41.0
18	38.40038133056002	40.0	37.0	41.0	34.0	41.0
19	38.40595496967814	40.0	37.0	41.0	34.0	41.0
20	38.25964244069567	40.0	37.0	41.0	34.0	41.0
21	38.243941256184804	40.0	37.0	41.0	34.0	41.0
22	38.22549152023483	40.0	37.0	41.0	34.0	41.0
23	38.24731335287259	40.0	37.0	41.0	34.0	41.0
24	38.16883894524057	40.0	37.0	41.0	34.0	41.0
25	38.090670682568195	40.0	36.0	41.0	34.0	41.0
26	37.9512666747705	40.0	36.0	41.0	34.0	41.0
27	37.92651395435739	40.0	36.0	41.0	34.0	41.0
28	37.916397664294045	40.0	36.0	41.0	34.0	41.0
29	37.920789493825325	40.0	36.0	41.0	34.0	41.0
30	37.78740033405818	40.0	36.0	41.0	33.0	41.0
31	37.80281563320232	40.0	36.0	41.0	33.0	41.0
32	37.7645835303016	40.0	36.0	41.0	33.0	41.0
33	37.729021191534194	40.0	36.0	41.0	33.0	41.0
34	37.64535807704948	40.0	36.0	41.0	33.0	41.0
35	37.608415834897826	40.0	36.0	41.0	33.0	41.0
36	37.52516691653498	40.0	35.0	41.0	33.0	41.0
37	37.500171081006854	40.0	35.0	41.0	33.0	41.0
38	37.43199755084032	40.0	35.0	41.0	33.0	41.0
39	37.37033185213198	40.0	35.0	41.0	33.0	41.0
40	37.276383617642956	40.0	35.0	41.0	33.0	41.0
41	37.18138188432223	39.0	35.0	41.0	33.0	41.0
42	37.16079363578655	39.0	35.0	41.0	33.0	41.0
43	37.09635012178266	39.0	35.0	41.0	32.0	41.0
44	37.03982135541179	39.0	35.0	41.0	32.0	41.0
45	36.93801014780499	39.0	35.0	41.0	32.0	41.0
46	36.87686669638074	39.0	35.0	41.0	32.0	41.0
47	36.82407920150191	39.0	35.0	41.0	32.0	41.0
48	36.7534970308441	39.0	35.0	41.0	32.0	41.0
49	36.71025405529518	39.0	35.0	41.0	32.0	41.0
50	36.5938514386562	39.0	35.0	41.0	31.0	41.0
51	35.63530481683077	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	4.0
10	10.0
11	15.0
12	16.0
13	12.0
14	15.0
15	23.0
16	30.0
17	40.0
18	81.0
19	176.0
20	249.0
21	419.0
22	675.0
23	936.0
24	1338.0
25	1939.0
26	2666.0
27	3252.0
28	3810.0
29	4463.0
30	5656.0
31	7197.0
32	9539.0
33	13626.0
34	28602.0
35	36103.0
36	30312.0
37	45644.0
38	84043.0
39	163167.0
40	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.771453783366425	22.056168595830126	24.615180287866302	11.557197332937145
2	31.57142406929681	25.014519374924028	24.81304897869141	18.60100757708775
3	32.667242939531874	24.097210028948705	25.99125686012327	17.244290171396155
4	29.30685179432461	26.196779174939334	25.53361516678147	18.962753863954582
5	26.150182111229668	29.75031177262434	24.05061296523904	20.048893150906956
6	27.213810739385096	33.84905252637124	23.162117262523804	15.775019471719858
7	79.8403544078121	6.385598580928069	8.581963559745539	5.192083451514292
8	80.14537383451064	5.9398875367486506	8.201983639253188	5.7127549894875225
9	74.0089682464647	7.975976624931905	10.564927493168016	7.450127635435379
10	42.613577528959965	25.117393085626045	17.64678075068545	14.622248634728544
11	32.55468964554717	24.888009472485223	23.621559808569355	18.935741073398255
12	30.390064695633384	21.681366126861068	27.52625868348663	20.40231049401892
13	26.528586285606238	23.041009918196266	28.883426302354167	21.546977493843336
14	20.59094981473728	27.24937758028426	29.43628808240702	22.72338452257144
15	20.613460473534218	25.073497300972008	33.53457862297798	20.778463602515792
16	22.98270731191219	25.492645767771037	30.68923135104472	20.83541556927205
17	22.603852924359686	25.475987880261304	27.962065037795398	23.958094157583616
18	23.317891021398633	27.150555788165697	28.020142537491505	21.511410652944168
19	26.113939950566596	26.709121769157697	25.519883664915337	21.657054615360373
20	27.03935313370881	27.273238878609025	26.237523467361797	19.44988452032037
21	25.450775942408733	26.9283755858399	27.37881386836667	20.242034603384703
22	23.188904946492165	25.656298257224798	27.0850497710666	24.06974702521644
23	23.812225088579442	25.674531890850318	28.0996051630447	22.41363785752554
24	24.54179554018828	24.313987673163243	27.933251394535315	23.210965392113163
25	23.71948117433605	25.75602047569524	26.28772223647897	24.23677611348974
26	21.357437746773098	27.008738637744973	27.606396628803736	24.02742698667819
27	22.76075221617436	26.47095899908607	27.441618606410135	23.32667017832944
28	20.505859524484844	27.121066825141703	28.114011984674743	24.25906166569871
29	22.648649135365595	25.17479526555824	28.322685791722378	23.85386980735378
30	22.749271780187918	24.871126478387517	27.886204117649704	24.493397623774857
31	23.88043238473417	26.85904275674532	25.69028935200818	23.570235506512333
32	25.116942872450103	25.941733410770002	25.74386471994489	23.197458996835003
33	23.907220068702532	25.68533700707285	26.62920893042856	23.778233993796064
34	22.83908930878771	25.762098353570416	28.60632009256383	22.792492245078044
35	22.419265522224773	24.550799803707054	28.27676404777662	24.75317062629155
36	21.946541687489027	27.732906531242545	28.25650445485938	22.064047326409057
37	23.715879468928538	27.16541282297168	26.964617746502967	22.154089961596814
38	23.82438084432979	25.97977642413683	25.887032509893437	24.308810221639945
39	23.180125789561355	24.51095593763647	28.06809024072899	24.240828032073185
40	23.2688177852213	24.450852478648642	29.812666297491862	22.467663438638194
41	21.029682554689646	25.88117973860623	28.79540962645813	24.293728080245998
42	23.074550799803706	24.883732447313804	28.445593988753675	23.596122764128815
43	23.316540381870816	23.796692734009554	28.20698100550611	24.679785878613522
44	21.9938140709626	24.33829918466394	27.522206764903185	26.145679979470277
45	22.569411616400366	23.489197134843348	27.12376810419734	26.81762314455895
46	23.101788696948006	25.14237991689065	27.2491524736963	24.506678912465052
47	21.969952772637843	24.465034193690713	29.336115650760636	24.22889738291081
48	21.566111553820736	22.89986808753945	30.1176857241904	25.416334634449413
49	22.59777504648451	22.54419967854779	30.435086013227263	24.422939261740435
50	20.68504436850849	22.521463913162883	30.180040249057928	26.6134514692707
51	20.603555783663566	22.970776662749813	28.33078962888928	28.094877924697343
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	299.0
1	586.5
2	874.0
3	1214.0
4	1554.0
5	1131.5
6	709.0
7	736.5
8	764.0
9	743.5
10	723.0
11	715.5
12	708.0
13	731.0
14	754.0
15	747.5
16	741.0
17	762.5
18	784.0
19	904.0
20	1024.0
21	979.5
22	935.0
23	1188.0
24	1441.0
25	1755.0
26	2916.0
27	3763.0
28	4356.0
29	4949.0
30	5752.5
31	6556.0
32	7153.0
33	7750.0
34	9334.5
35	10919.0
36	11437.5
37	11956.0
38	13733.0
39	15510.0
40	16684.0
41	17858.0
42	19864.0
43	21870.0
44	24581.5
45	27293.0
46	31670.5
47	36048.0
48	39968.0
49	43888.0
50	43435.0
51	42982.0
52	38017.0
53	33052.0
54	29760.0
55	26468.0
56	25204.5
57	23941.0
58	23197.0
59	22453.0
60	22106.5
61	21760.0
62	19870.5
63	17981.0
64	15094.0
65	12207.0
66	9740.0
67	7273.0
68	6070.5
69	4868.0
70	4088.0
71	3308.0
72	2958.5
73	2609.0
74	2210.5
75	1363.0
76	914.0
77	669.0
78	424.0
79	375.5
80	327.0
81	202.0
82	77.0
83	48.5
84	20.0
85	16.5
86	13.0
87	8.0
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	444234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.53877015966399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.92642028807992	23.07858488001562
2	10.714800759222207	6.972928784218493
3	3.876600359889626	3.784194243339577
4	1.939588118158539	2.524472479247036
5	1.240109221210014	2.017581446091628
6	0.9522271943956874	1.8590581090933769
7	0.8191618869137292	1.8658164253289748
8	0.7247432576908432	1.8865803429414782
9	0.6385427692320409	1.869965676464107
>10	8.039290723715116	48.92069572318777
>50	0.0873066722094487	1.9745055936673375
>100	0.038414935772157426	2.3742702505804183
>500	0.0013969067553511792	0.33612957792748527
>1k	0.0013969067553511792	0.5352164678967058
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	1251	0.2816083415497238	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	1104	0.24851767311822145	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	827	0.1861631482506967	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	652	0.1467694953560511	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.251065879694035E-4	0.0	0.0	0.19584273153338105	0.0
2	2.251065879694035E-4	0.0	0.0	0.8990757123497977	0.0
3	2.251065879694035E-4	0.0	0.0	1.2720773286150993	0.0
4	2.251065879694035E-4	0.0	0.0	1.798601637875534	0.0
5	2.251065879694035E-4	0.0	0.0	3.2644957387322897	0.0
6	2.251065879694035E-4	0.0	0.0	4.052819009801141	0.0
7	2.251065879694035E-4	0.0	0.0	4.890215517047322	0.0
8	2.251065879694035E-4	0.0	0.0	6.16274305883836	0.0
9	2.251065879694035E-4	0.0	0.0	6.629839228874872	0.0
10	2.251065879694035E-4	0.0	0.0	7.685364019863405	0.0
11	2.251065879694035E-4	0.0	0.0	9.229595213333514	0.0
12	2.251065879694035E-4	0.0	0.0	10.49176785207796	0.0
13	2.251065879694035E-4	0.0	0.0	10.99735724865724	0.0
14	2.251065879694035E-4	0.0	0.0	11.192524660426711	0.0
15	2.251065879694035E-4	0.0	0.0	11.577006712678454	0.0
16	2.251065879694035E-4	0.0	0.0	12.383788723960796	0.0
17	2.251065879694035E-4	0.0	0.0	13.266206548800858	0.0
18	2.251065879694035E-4	0.0	0.0	14.194771224174646	0.0
19	2.251065879694035E-4	0.0	0.0	14.782974738538698	0.0
20	2.251065879694035E-4	0.0	0.0	15.424303407663528	0.0
21	2.251065879694035E-4	0.0	0.0	16.15522449880018	0.0
22	2.251065879694035E-4	0.0	0.0	16.931842227294624	0.0
23	2.251065879694035E-4	0.0	0.0	17.64295393868997	0.0
24	2.251065879694035E-4	0.0	0.0	18.241512356100614	0.0
25	2.251065879694035E-4	0.0	0.0	18.768711985124956	0.0
26	2.251065879694035E-4	0.0	0.0	19.230405597050204	0.0
27	2.251065879694035E-4	0.0	0.0	19.713034121656605	0.0
28	2.251065879694035E-4	0.0	0.0	20.22830310151857	0.0
29	2.251065879694035E-4	0.0	0.0	20.742446548440686	0.0
30	2.251065879694035E-4	0.0	0.0	21.345732204198686	0.0
31	2.251065879694035E-4	0.0	0.0	21.8592003313569	0.0
32	2.251065879694035E-4	0.0	0.0	22.389776559200783	0.0
33	4.50213175938807E-4	0.0	0.0	22.896941701895848	0.0
34	4.50213175938807E-4	0.0	0.0	23.397128540363862	0.0
35	6.753197639082105E-4	0.0	0.0	23.93715924490246	0.0
36	6.753197639082105E-4	0.0	0.0	24.48596910637187	0.0
37	6.753197639082105E-4	0.0	0.0	25.003264045525555	0.0
38	6.753197639082105E-4	0.0	0.0	25.540143257832582	0.0
39	6.753197639082105E-4	0.0	0.0	26.098857809172642	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTACGA	60	0.0	45.000004	10
GCGTAAG	30	2.1624837E-6	45.000004	1
TGATCGC	20	7.028608E-4	45.0	37
TCACGAC	50	2.1827873E-11	45.0	25
GTTTACG	20	7.028608E-4	45.0	10
CACGATC	20	7.028608E-4	45.0	8
GCGTTAG	25	3.8869286E-5	45.0	1
CGGATAT	20	7.028608E-4	45.0	10
ACGGATA	20	7.028608E-4	45.0	9
CGATCAT	20	7.028608E-4	45.0	10
CGATATT	20	7.028608E-4	45.0	33
CTAGGGT	50	2.1827873E-11	45.0	4
TAGTCCG	25	3.8869286E-5	45.0	1
TGCACCG	20	7.028608E-4	45.0	1
AATCGTT	25	3.8869286E-5	45.0	22
CTTACGA	20	7.028608E-4	45.0	29
CGGGGTA	110	0.0	40.90909	6
CTAGCGG	55	6.002665E-11	40.90909	2
CGGTCTA	50	1.0786607E-9	40.5	31
CTCACGT	45	1.9237632E-8	40.0	45
>>END_MODULE