##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551012_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335986
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0676129362533	33.0	31.0	34.0	30.0	34.0
2	32.20901763763966	33.0	31.0	34.0	30.0	34.0
3	32.238771853589135	34.0	31.0	34.0	30.0	34.0
4	35.79697368342728	37.0	35.0	37.0	35.0	37.0
5	35.84053204597811	37.0	35.0	37.0	35.0	37.0
6	35.84919907377093	37.0	35.0	37.0	35.0	37.0
7	36.0189888864417	37.0	35.0	37.0	35.0	37.0
8	35.75505229384558	37.0	35.0	37.0	35.0	37.0
9	37.479731298327906	39.0	37.0	39.0	35.0	39.0
10	37.07944378634824	39.0	37.0	39.0	33.0	39.0
11	37.072806605037115	39.0	37.0	39.0	34.0	39.0
12	37.04347502574512	39.0	37.0	39.0	34.0	39.0
13	37.03780812295751	39.0	37.0	39.0	33.0	39.0
14	38.22261344222676	40.0	37.0	41.0	34.0	41.0
15	38.36518485889293	40.0	38.0	41.0	34.0	41.0
16	38.45847148393088	40.0	38.0	41.0	34.0	41.0
17	38.437137261671616	40.0	38.0	41.0	34.0	41.0
18	38.41285946438244	40.0	37.0	41.0	34.0	41.0
19	38.35530349478847	40.0	37.0	41.0	34.0	41.0
20	38.21455953521873	40.0	37.0	41.0	34.0	41.0
21	38.19875530528058	40.0	37.0	41.0	34.0	41.0
22	38.187460191793704	40.0	37.0	41.0	34.0	41.0
23	38.20599370211854	40.0	37.0	41.0	34.0	41.0
24	38.113992844939965	40.0	37.0	41.0	34.0	41.0
25	38.03883495145631	40.0	36.0	41.0	34.0	41.0
26	37.8987219705583	40.0	36.0	41.0	34.0	41.0
27	37.84466614680374	40.0	36.0	41.0	33.0	41.0
28	37.82177233575209	40.0	36.0	41.0	33.0	41.0
29	37.80972421469942	40.0	36.0	41.0	33.0	41.0
30	37.70216020905633	40.0	36.0	41.0	33.0	41.0
31	37.690251974784665	40.0	36.0	41.0	33.0	41.0
32	37.605227598769	40.0	36.0	41.0	33.0	41.0
33	37.54886513128523	40.0	36.0	41.0	33.0	41.0
34	37.46564142553559	40.0	36.0	41.0	33.0	41.0
35	37.43627710678421	40.0	36.0	41.0	33.0	41.0
36	37.36607180061074	40.0	36.0	41.0	33.0	41.0
37	37.313129118475175	40.0	35.0	41.0	33.0	41.0
38	37.22625049853268	40.0	35.0	41.0	32.0	41.0
39	37.190921645544755	40.0	35.0	41.0	32.0	41.0
40	37.1141238027775	39.0	35.0	41.0	32.0	41.0
41	37.01248861559708	39.0	35.0	41.0	32.0	41.0
42	36.98191293684856	39.0	35.0	41.0	32.0	41.0
43	36.911796920109765	39.0	35.0	41.0	32.0	41.0
44	36.833740096313534	39.0	35.0	41.0	31.0	41.0
45	36.72445578089563	39.0	35.0	41.0	31.0	41.0
46	36.63356806533606	39.0	35.0	41.0	31.0	41.0
47	36.58290821641378	39.0	35.0	41.0	31.0	41.0
48	36.503643008934894	39.0	35.0	41.0	31.0	41.0
49	36.460673956652954	39.0	35.0	41.0	31.0	41.0
50	36.33092450280667	39.0	35.0	41.0	31.0	41.0
51	35.337400963135366	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	3.0
9	12.0
10	11.0
11	3.0
12	4.0
13	8.0
14	11.0
15	11.0
16	19.0
17	40.0
18	59.0
19	135.0
20	228.0
21	359.0
22	544.0
23	852.0
24	1279.0
25	1868.0
26	2529.0
27	2920.0
28	3241.0
29	3756.0
30	4567.0
31	5751.0
32	7421.0
33	10699.0
34	19656.0
35	26498.0
36	24109.0
37	36427.0
38	66469.0
39	116399.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.35449096093289	24.665611067127795	26.958861381128973	12.021036590810331
2	32.30491746679921	25.77518110873667	23.949807432452545	17.97009399201157
3	31.754299286279785	24.066776591881805	26.64218151946807	17.536742602370335
4	29.62831784657694	24.898954123088462	26.646348359753087	18.82637967058151
5	26.22906906835404	29.275326948146652	24.927526742185684	19.568077241313624
6	26.22103301923294	33.83146916835821	24.753709976010903	15.193787836397945
7	79.62504390063872	5.781788526902907	9.384914847642461	5.208252724815915
8	80.298583869566	5.480585500586334	8.809890888310823	5.41093974153685
9	73.95784348157363	8.008369396343895	10.97009994464055	7.063687177441917
10	40.08500354181424	24.303393593780694	19.680879560457875	15.93072330394719
11	30.408409874220947	25.95792681837934	23.78819355568387	19.845469751715843
12	28.281535540171316	21.650306858023846	28.83989213836291	21.22826546344193
13	24.621263981237313	24.899251754537392	30.190543653604614	20.28894061062068
14	20.393409249194907	27.965153309959344	29.52176578786021	22.119671652985538
15	20.124052787913783	26.816593548540713	33.0867952831368	19.97255838040871
16	22.707493764621145	25.52487306018703	30.72628026167757	21.041352913514253
17	21.99228539284375	26.288297726690995	27.4133446036442	24.30607227682106
18	23.312876131743586	26.393659259612008	28.673218526962437	21.620246081681973
19	24.30071491074033	28.866976600215484	26.500508949777668	20.33179953926652
20	24.754305238908763	27.129999464263392	28.1407558648277	19.974939432000145
21	22.67475430523891	28.18272189912675	28.50059228658337	20.641931509050973
22	21.722928931562624	25.64809248004381	28.0011667152798	24.62781187311376
23	21.690784735078246	26.301691141892817	28.415767323638484	23.591756799390453
24	22.01192906847309	25.507610436149125	29.4387266136089	23.041733881768884
25	21.307733060306084	27.89669807670558	26.805581184930325	23.989987678058014
26	20.741935675891256	28.492258606013344	27.82348074026894	22.942324977826456
27	22.55213014827999	27.304113861887103	27.804729958986385	22.339026030846522
28	19.08353324245653	27.05023423595031	30.019405570470198	23.846826951122964
29	20.46424553404011	25.17098926740995	28.743757180358703	25.621008018191233
30	23.039650461626376	25.973403653723665	26.886536939039125	24.10040894561083
31	22.630109587899497	26.95618269808861	25.96268892156221	24.451018792449688
32	22.266701588756675	26.849333007922947	25.752858750066963	25.13110665325341
33	23.764085408320586	26.877607995571246	25.40403469192169	23.954271904186484
34	20.511568934419884	26.226092753864744	27.050531867399236	26.21180644431613
35	21.948831201300052	25.354925502848335	26.245438798045157	26.45080449780646
36	22.610168280821224	27.23982546891835	26.95856374968005	23.19144250058038
37	21.229158357788716	27.189525754049278	28.646431696558782	22.93488419160322
38	21.374997767764135	27.50084824962945	26.595453381986157	24.528700600620265
39	22.859881066473008	24.536736649741357	27.727643413713665	24.875738870071967
40	22.461650187805443	25.439750465793214	27.301137547397808	24.797461799003532
41	19.419856779746773	27.05350818188853	27.793419963927068	25.733215074437627
42	21.362794878358027	26.036799152345637	28.722625347484716	23.877780621811624
43	22.646776949039545	24.21469942199973	29.272945896555214	23.865577732405516
44	21.462501413749383	25.06919931187609	27.19845469751716	26.26984457685737
45	22.734875857922653	24.2700588715006	25.625174858476246	27.369890412100506
46	23.021197311792754	26.08442018417434	25.88411421904484	25.01026828498806
47	20.850868786199424	25.04538879596174	29.071747037078925	25.031995380759913
48	20.618716256034478	24.31946569202288	29.682784401731027	25.379033650211618
49	21.718762091277615	23.02238783758847	30.34203806110969	24.916812010024227
50	20.03893019351997	23.053341508277132	29.56164840201675	27.346079896186147
51	20.38031346544201	23.58044680433113	27.095474216187583	28.943765514039278
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	240.0
1	452.0
2	664.0
3	1132.0
4	1600.0
5	1175.5
6	751.0
7	704.5
8	658.0
9	713.0
10	768.0
11	718.0
12	668.0
13	681.5
14	695.0
15	707.5
16	720.0
17	709.0
18	698.0
19	832.0
20	966.0
21	955.5
22	945.0
23	1127.5
24	1310.0
25	1631.0
26	2316.0
27	2680.0
28	3522.0
29	4364.0
30	4879.0
31	5394.0
32	6115.0
33	6836.0
34	7697.5
35	8559.0
36	9128.5
37	9698.0
38	10418.0
39	11138.0
40	12431.0
41	13724.0
42	15481.5
43	17239.0
44	18842.0
45	20445.0
46	22174.0
47	23903.0
48	27112.0
49	30321.0
50	32829.5
51	35338.0
52	34279.0
53	33220.0
54	28774.0
55	24328.0
56	21321.5
57	18315.0
58	16110.0
59	13905.0
60	13287.0
61	12669.0
62	11274.5
63	9880.0
64	8552.0
65	7224.0
66	5796.0
67	4368.0
68	3930.5
69	3493.0
70	2989.5
71	2486.0
72	2078.0
73	1670.0
74	1342.5
75	797.5
76	580.0
77	419.5
78	259.0
79	224.0
80	189.0
81	129.5
82	70.0
83	50.5
84	31.0
85	17.5
86	4.0
87	3.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	335986.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.71254286136972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.7742931916191	29.694773233007993
2	10.337247814666812	8.210367938171125
3	3.570723531876948	4.254075339172943
4	1.805831164211084	2.8685659003651947
5	1.2422944084330874	2.4667334970669463
6	0.9137632028253179	2.1772716214405743
7	0.7068136622275613	1.9648557499349608
8	0.5955721884537202	1.89213488488065
9	0.5660613378547126	2.0231761627547606
>10	5.3823522981215834	39.3003585327598
>50	0.06499323147529498	1.749409301635619
>100	0.038542497735349346	2.903977423679233
>500	0.0015114704994254647	0.49430041513018774
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	883	0.26280856940467756	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	764	0.22739042698207662	No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	498	0.148220461566851	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	418	0.12440994565249744	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	413	0.12292178840785033	No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	402	0.1196478424696267	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	356	0.1059567958188734	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCC	346	0.10298048132957921	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12530283999928568	0.0
2	0.0	0.0	0.0	0.7107439000434542	0.0
3	0.0	0.0	0.0	1.1610602822736662	0.0
4	0.0	0.0	0.0	1.7973963200847654	0.0
5	0.0	0.0	0.0	3.434666920645503	0.0
6	0.0	0.0	0.0	4.484710672468496	0.0
7	0.0	0.0	0.0	5.394570011845731	0.0
8	0.0	0.0	0.0	6.782723089652545	0.0
9	0.0	0.0	0.0	7.2872083955879114	0.0
10	0.0	0.0	0.0	8.460173935818755	0.0
11	2.976314489294197E-4	0.0	0.0	10.33941890435911	0.0
12	2.976314489294197E-4	0.0	0.0	11.778764591381783	0.0
13	2.976314489294197E-4	0.0	0.0	12.332359086390504	0.0
14	2.976314489294197E-4	0.0	0.0	12.531176894275356	0.0
15	2.976314489294197E-4	0.0	0.0	12.928217247147202	0.0
16	2.976314489294197E-4	0.0	0.0	13.874685254742758	0.0
17	2.976314489294197E-4	0.0	0.0	14.994970028513093	0.0
18	2.976314489294197E-4	0.0	0.0	16.23668843344663	0.0
19	2.976314489294197E-4	0.0	0.0	16.975112058240523	0.0
20	5.952628978588394E-4	0.0	0.0	17.687344115528624	0.0
21	5.952628978588394E-4	0.0	0.0	18.564463995523624	0.0
22	5.952628978588394E-4	0.0	0.0	19.46450149708619	0.0
23	5.952628978588394E-4	0.0	0.0	20.466031322733684	0.0
24	5.952628978588394E-4	0.0	0.0	21.229455989237646	0.0
25	5.952628978588394E-4	0.0	0.0	21.898233854982053	0.0
26	5.952628978588394E-4	0.0	0.0	22.52147410904026	0.0
27	5.952628978588394E-4	0.0	0.0	23.145309625996322	0.0
28	5.952628978588394E-4	0.0	0.0	23.784919609745643	0.0
29	5.952628978588394E-4	0.0	0.0	24.502806664563405	0.0
30	5.952628978588394E-4	0.0	0.0	25.22932503140012	0.0
31	5.952628978588394E-4	0.0	0.0	25.917151309876008	0.0
32	5.952628978588394E-4	0.0	0.0	26.56658313144	0.0
33	5.952628978588394E-4	0.0	0.0	27.189228122600348	0.0
34	5.952628978588394E-4	0.0	0.0	27.786276809152763	0.0
35	5.952628978588394E-4	0.0	0.0	28.380646812664814	0.0
36	5.952628978588394E-4	0.0	0.0	29.004779961069808	0.0
37	8.92894346788259E-4	0.0	0.0	29.62831784657694	0.0
38	8.92894346788259E-4	0.0	0.0	30.246498366003344	0.0
39	8.92894346788259E-4	0.0	0.0	30.852773627472573	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCAACG	25	3.8848935E-5	45.0	1
GTCGAAC	20	7.026151E-4	45.0	20
GCGCGAC	55	1.8189894E-12	45.0	9
TTCACGG	20	7.026151E-4	45.0	2
CCAGCGC	20	7.026151E-4	45.0	42
TATTGCG	25	3.8848935E-5	45.0	1
TCGGAAA	25	3.8848935E-5	45.0	42
CGGATGG	20	7.026151E-4	45.0	2
TTGCGGT	20	7.026151E-4	45.0	12
CGAATAT	45	3.8380676E-10	45.0	14
TCATCGA	20	7.026151E-4	45.0	16
CCGGTAT	20	7.026151E-4	45.0	19
CGTCATA	20	7.026151E-4	45.0	38
TCCGGTA	20	7.026151E-4	45.0	18
GCCAATT	30	2.1608994E-6	44.999996	42
GGCGATA	140	0.0	41.785713	8
GCGATAC	60	3.6379788E-12	41.249996	9
CGTTTTT	580	0.0	41.12069	1
CCATCTA	55	6.002665E-11	40.909092	13
TAAGGGA	165	0.0	40.90909	4
>>END_MODULE