##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551011_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	646339
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06185299045857	33.0	31.0	34.0	30.0	34.0
2	32.18481942138723	33.0	31.0	34.0	30.0	34.0
3	32.24053321863604	34.0	31.0	34.0	30.0	34.0
4	35.79910851735699	37.0	35.0	37.0	35.0	37.0
5	35.83750013537787	37.0	35.0	37.0	35.0	37.0
6	35.85833285628749	37.0	35.0	37.0	35.0	37.0
7	36.05587315634675	37.0	35.0	37.0	35.0	37.0
8	35.78015716210843	37.0	35.0	37.0	35.0	37.0
9	37.546742498905374	39.0	37.0	39.0	35.0	39.0
10	37.122978808334324	39.0	37.0	39.0	33.0	39.0
11	37.07103547828616	39.0	37.0	39.0	34.0	39.0
12	37.12866777341302	39.0	37.0	39.0	34.0	39.0
13	37.18560848099836	39.0	37.0	39.0	34.0	39.0
14	38.404889694107894	40.0	38.0	41.0	34.0	41.0
15	38.53032696464239	40.0	38.0	41.0	34.0	41.0
16	38.62125448100765	40.0	38.0	41.0	35.0	41.0
17	38.579816474017505	40.0	38.0	41.0	34.0	41.0
18	38.575851062677636	40.0	38.0	41.0	34.0	41.0
19	38.57081964727488	40.0	38.0	41.0	34.0	41.0
20	38.42675438121481	40.0	38.0	41.0	34.0	41.0
21	38.434949770940634	40.0	38.0	41.0	34.0	41.0
22	38.427281968131275	40.0	38.0	41.0	34.0	41.0
23	38.42973578880433	40.0	38.0	41.0	34.0	41.0
24	38.37243149492758	40.0	38.0	41.0	34.0	41.0
25	38.292165566366876	40.0	37.0	41.0	34.0	41.0
26	38.16344054745265	40.0	37.0	41.0	34.0	41.0
27	38.12265235425992	40.0	37.0	41.0	34.0	41.0
28	38.11718154095606	40.0	37.0	41.0	34.0	41.0
29	38.12627429259258	40.0	37.0	41.0	34.0	41.0
30	38.03681504597433	40.0	37.0	41.0	34.0	41.0
31	38.06383028101352	40.0	37.0	41.0	34.0	41.0
32	38.01629176020633	40.0	37.0	41.0	34.0	41.0
33	37.99950954530053	40.0	37.0	41.0	34.0	41.0
34	37.972867179606986	40.0	37.0	41.0	34.0	41.0
35	37.976465910304036	40.0	37.0	41.0	34.0	41.0
36	37.90915138959586	40.0	37.0	41.0	33.0	41.0
37	37.857104398775256	40.0	37.0	41.0	33.0	41.0
38	37.8096293121721	40.0	37.0	41.0	33.0	41.0
39	37.77947949914828	40.0	37.0	41.0	33.0	41.0
40	37.750672634639095	40.0	37.0	41.0	33.0	41.0
41	37.681688711341884	40.0	37.0	41.0	33.0	41.0
42	37.6627713939589	40.0	37.0	41.0	33.0	41.0
43	37.60283844855409	40.0	36.0	41.0	33.0	41.0
44	37.55077289162498	40.0	36.0	41.0	33.0	41.0
45	37.450837718287154	40.0	36.0	41.0	33.0	41.0
46	37.39524150639215	40.0	36.0	41.0	33.0	41.0
47	37.356359433671805	40.0	36.0	41.0	32.0	41.0
48	37.31713388794425	40.0	36.0	41.0	32.0	41.0
49	37.29529859717579	39.0	36.0	41.0	32.0	41.0
50	37.18262552623314	39.0	35.0	41.0	32.0	41.0
51	36.25964238580683	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	7.0
9	4.0
10	7.0
11	8.0
12	8.0
13	11.0
14	13.0
15	23.0
16	30.0
17	46.0
18	107.0
19	172.0
20	329.0
21	518.0
22	929.0
23	1341.0
24	1910.0
25	2533.0
26	3367.0
27	4285.0
28	4864.0
29	5958.0
30	7487.0
31	9921.0
32	13174.0
33	18039.0
34	32379.0
35	43202.0
36	42687.0
37	65299.0
38	128648.0
39	258801.0
40	231.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.39731626901672	22.629920212148733	26.35381742398339	9.618946094851154
2	31.641445123998395	24.752181130954497	25.61086364895202	17.99551009609508
3	31.385387544307243	24.869147614487133	26.90430254092667	16.841162300278956
4	27.986706666315975	25.930045997533803	26.956751797431377	19.126495538718846
5	26.3674325702147	29.37761762790115	24.46502531953046	19.789924482353687
6	26.061555932722612	34.36664041625215	24.232175375460866	15.33962827556437
7	80.72451144059077	5.673802756757676	8.787493869316258	4.814191933335294
8	81.74549269036837	5.574474076297423	7.540160813443101	5.13987241989111
9	75.64111093404544	7.387299853482461	9.627146126104105	7.344443086367989
10	37.339847974514925	30.996891724002417	17.848528403825238	13.81473189765742
11	26.31977955840511	26.067589917984215	27.70078859545842	19.911841928152256
12	24.194888440895568	23.462146025537685	31.226337881514187	21.11662765205256
13	23.217228110944877	24.99431412927272	33.058039202338094	18.730418557444313
14	20.203020396417358	28.531003080426835	30.50024832170115	20.765728201454657
15	19.193642964450543	27.60409011370194	33.33297232566811	19.869294596179405
16	21.296254751763392	26.861445773812193	30.66270177105203	21.17959770337238
17	20.99734040495777	27.536169100116194	28.80918527274387	22.657305222182167
18	21.688773228909287	26.9316875509601	30.706486843591367	20.673052376539246
19	21.755456501928556	28.73786047260029	28.504082223105833	21.002600802365322
20	22.792837814212046	28.921974381864622	29.42140270044048	18.863785103482847
21	22.011668799190517	28.504855810959885	30.018767241339294	19.4647081485103
22	20.965159150229216	25.731233919042484	29.028420070582158	24.275186860146146
23	20.086672783167966	27.620180741066218	29.91448759861311	22.378658877152702
24	21.249375327807854	26.05861629887721	30.237692604035963	22.45431576927897
25	19.55413490443869	29.652396033660356	27.750452935688546	23.043016126212407
26	19.173529680245196	28.819241914846543	29.202786772885435	22.804441632022822
27	21.598882320268466	27.783098343129538	28.587320276201805	22.03069906040019
28	18.54970843473781	27.387176079425814	30.754139855400958	23.308975630435423
29	19.54454241504845	25.280696352842703	29.946514135770858	25.22824709633799
30	21.79955100960951	26.306628564886232	28.521410591036595	23.37240983446767
31	20.434632599920473	26.667429940015996	29.101601481575457	23.79633597848807
32	20.60528608052431	27.481553797620133	28.59289010875098	23.320270013104576
33	20.61379554691888	26.058461581306403	28.03621628897529	25.291526582799428
34	18.284367800798034	25.233507493745545	31.095137381467	25.386987323989423
35	19.678527831370225	25.62741842902873	29.240383142592353	25.453670597008692
36	20.739735649558515	26.490742474150565	27.90764598763188	24.861875888659046
37	20.163412698289907	26.84566458158954	31.40550082851259	21.585421891607965
38	18.513659240738995	27.607184465118152	29.690147120938082	24.189009173204774
39	19.810811354413087	24.685962010647664	29.991536948876675	25.511689686062578
40	19.890490903380424	25.22948483690447	30.935004695678277	23.94501956403683
41	18.352907684667024	26.605697629262664	28.856528849411838	26.18486583665847
42	19.82968689805195	25.586727707905606	29.614799663953434	24.96878573008901
43	20.84819266669658	24.329183292358962	29.631354444030144	25.19126959691431
44	19.6259238572947	24.62593159317324	29.56884854542276	26.179296004109297
45	20.443142066315044	24.06322378813595	29.10995623039922	26.38367791514979
46	21.523225428142197	25.366874039784076	28.676747032130194	24.43315349994353
47	18.72392041947028	24.528768958704333	32.16377164305418	24.5835389787712
48	18.944238240304237	24.43222519451867	30.664248946760136	25.95928761841696
49	19.929170296083015	22.90129483135011	32.124782815209976	25.044752057356902
50	19.388122950959172	22.232141337595287	31.64686023897676	26.73287547246878
51	18.355383165799992	22.498410276959923	29.338319364915318	29.807887192324774
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	205.0
1	408.0
2	611.0
3	1149.5
4	1688.0
5	1235.5
6	783.0
7	750.0
8	717.0
9	751.0
10	785.0
11	764.0
12	743.0
13	803.0
14	863.0
15	906.5
16	950.0
17	960.0
18	970.0
19	1181.0
20	1392.0
21	1676.5
22	1961.0
23	2386.0
24	2811.0
25	3512.5
26	4955.5
27	5697.0
28	7175.5
29	8654.0
30	10621.0
31	12588.0
32	14378.5
33	16169.0
34	18430.5
35	20692.0
36	21851.5
37	23011.0
38	25207.5
39	27404.0
40	29649.0
41	31894.0
42	36030.0
43	40166.0
44	45303.5
45	50441.0
46	59157.5
47	67874.0
48	71182.5
49	74491.0
50	72165.0
51	69839.0
52	59886.5
53	49934.0
54	42406.0
55	34878.0
56	30709.5
57	26541.0
58	23327.5
59	20114.0
60	18248.0
61	16382.0
62	14054.0
63	11726.0
64	10130.0
65	8534.0
66	6393.5
67	4253.0
68	3459.0
69	2665.0
70	2073.0
71	1481.0
72	1180.5
73	880.0
74	781.5
75	521.5
76	360.0
77	269.0
78	178.0
79	117.5
80	57.0
81	47.5
82	38.0
83	26.0
84	14.0
85	9.0
86	4.0
87	2.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	646339.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.819137465245056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.96620312963955	24.70981982157424
2	12.046120293957397	8.388710368763617
3	4.3957130494221754	4.5916481077680675
4	2.207349410605724	3.0743201024683344
5	1.3565823167024125	2.3617513084090955
6	0.9568875186723518	1.999079883085792
7	0.7414888399317973	1.807260129257124
8	0.6183784835844007	1.7225124340380027
9	0.5083011970989866	1.5928748329984415
>10	6.064194376465562	43.055528021383445
>50	0.08982613846679127	2.1055465439886145
>100	0.04446393853057886	2.836482710030286
>500	0.0017965227689122772	0.40784799359129925
>1k	0.002694784153368416	1.3466177426436647
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	1928	0.2982954765223822	TruSeq Adapter, Index 23 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	1549	0.2396575171852542	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGC	1465	0.22666124123718356	No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	1314	0.2032988880448186	TruSeq Adapter, Index 23 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG	1259	0.19478942165024857	No Hit
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	1096	0.1695704576081592	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	778	0.12037027009046336	No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	771	0.1192872470947908	TruSeq Adapter, Index 23 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0943514162072843E-4	0.0	0.0	0.1746761374449012	0.0
2	3.0943514162072843E-4	0.0	0.0	0.9108223393606142	0.0
3	3.0943514162072843E-4	0.0	0.0	1.3446504079128754	0.0
4	3.0943514162072843E-4	0.0	0.0	2.039796453563842	0.0
5	3.0943514162072843E-4	0.0	0.0	3.944988620522667	0.0
6	3.0943514162072843E-4	0.0	0.0	4.903773406834494	0.0
7	3.0943514162072843E-4	0.0	0.0	5.839195839953956	0.0
8	3.0943514162072843E-4	0.0	0.0	7.354499728470663	0.0
9	3.0943514162072843E-4	0.0	0.0	7.87141113254809	0.0
10	3.0943514162072843E-4	0.0	0.0	9.280733485059697	0.0
11	3.0943514162072843E-4	0.0	0.0	11.11676689786629	0.0
12	3.0943514162072843E-4	0.0	0.0	12.761724110722083	0.0
13	3.0943514162072843E-4	0.0	0.0	13.332631947012326	0.0
14	3.0943514162072843E-4	0.0	0.0	13.545213889305767	0.0
15	3.0943514162072843E-4	0.0	0.0	14.025147793959517	0.0
16	3.0943514162072843E-4	0.0	0.0	14.960415509508168	0.0
17	3.0943514162072843E-4	0.0	0.0	16.145397384344747	0.0
18	4.6415271243109265E-4	0.0	0.0	17.404798410741112	0.0
19	4.6415271243109265E-4	0.0	0.0	18.237797811984112	0.0
20	4.6415271243109265E-4	0.0	0.0	19.006434703770005	0.0
21	4.6415271243109265E-4	0.0	0.0	19.946498664013777	0.0
22	4.6415271243109265E-4	0.0	0.0	20.97660825046918	0.0
23	4.6415271243109265E-4	0.0	0.0	22.028068861696415	0.0
24	4.6415271243109265E-4	0.0	0.0	22.821924717524396	0.0
25	4.6415271243109265E-4	0.0	0.0	23.550489758470402	0.0
26	4.6415271243109265E-4	0.0	0.0	24.189937478629634	0.0
27	4.6415271243109265E-4	0.0	0.0	24.81530589984513	0.0
28	4.6415271243109265E-4	0.0	0.0	25.48739902744535	0.0
29	6.188702832414569E-4	0.0	0.0	26.165216705165555	0.0
30	6.188702832414569E-4	0.0	0.0	26.91590635873744	0.0
31	6.188702832414569E-4	0.0	0.0	27.657003522919087	0.0
32	6.188702832414569E-4	0.0	0.0	28.365145844518125	0.0
33	6.188702832414569E-4	0.0	0.0	29.031669139569175	0.0
34	6.188702832414569E-4	0.0	0.0	29.669414966449494	0.0
35	6.188702832414569E-4	0.0	0.0	30.348470384736185	0.0
36	6.188702832414569E-4	0.0	0.0	31.01406537436237	0.0
37	6.188702832414569E-4	0.0	0.0	31.699928365764716	0.0
38	6.188702832414569E-4	0.0	0.0	32.35685917142552	0.0
39	6.188702832414569E-4	0.0	0.0	33.03808063570356	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	80	0.0	45.000004	1
GATCGCG	20	7.030992E-4	45.000004	1
GATCGAG	20	7.030992E-4	45.000004	1
TTTCGCG	20	7.030992E-4	45.000004	1
ACTAGCG	20	7.030992E-4	45.000004	1
CCGTAAG	20	7.030992E-4	45.000004	1
ACGCTAG	20	7.030992E-4	45.000004	1
CGTGTAC	20	7.030992E-4	45.000004	18
ATACGCG	20	7.030992E-4	45.000004	1
GCGCTTC	20	7.030992E-4	45.000004	19
CGTCATA	40	6.8066583E-9	45.000004	38
TGTAACG	40	6.8066583E-9	45.000004	1
TGCCGTA	20	7.030992E-4	45.000004	42
ATTTCGC	20	7.030992E-4	45.000004	20
CGGGTTA	25	3.8889037E-5	45.0	6
TGCGAGC	25	3.8889037E-5	45.0	1
CTAGCGG	125	0.0	41.399998	2
TATTGCG	50	1.0804797E-9	40.5	1
GTCATAC	45	1.9261279E-8	40.0	39
GCGATAC	45	1.9261279E-8	40.0	9
>>END_MODULE